Biochemical methods: a concise guide for students and researchers
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| Format: | Book |
|---|---|
| Language: | English German |
| Published: |
Weinheim
Wiley-VCH
2002
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Überarb. engl. Ausg. von: Pingoud, Alfred: Arbeitsmethoden der Biochemie |
| Physical Description: | XIII, 360 S. graph. Darst. 1 CD-ROM (12 cm) |
| ISBN: | 3527302999 |
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| 245 | 1 | 0 | |a Biochemical methods |b a concise guide for students and researchers |c A. Pingoud ... |
| 264 | 1 | |a Weinheim |b Wiley-VCH |c 2002 | |
| 300 | |a XIII, 360 S. |b graph. Darst. |e 1 CD-ROM (12 cm) | ||
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| 337 | |b n |2 rdamedia | ||
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| 500 | |a Überarb. engl. Ausg. von: Pingoud, Alfred: Arbeitsmethoden der Biochemie | ||
| 650 | 7 | |a Biochemistry |2 cabt | |
| 650 | 7 | |a Biochemical Techniques |2 cabt | |
| 650 | 7 | |a Biochemie |2 gtt | |
| 650 | 4 | |a Biochimie - Méthodologie | |
| 650 | 4 | |a Biyokimya - Yöntembilim | |
| 650 | 4 | |a Biochemistry |x Methodology | |
| 650 | 4 | |a Biochemistry |x methods | |
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Record in the Search Index
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| adam_text |
IX
CONTENTS
PREFACE
V
1
1.1
1.1.1
1.1.2
1.1.3
1.2
1.2.1
1.2.2
1.3
1.3.1
1.3.2
1.4
1.4.1
1.4.2
1.4.3
1.5
1.5.1
1.5.2
1.6
BIOCHEMICAL
LITERATURE
1
ACCESSING
THE
BIOCHEMICAL
LITERATURE
1
TEXTBOOKS
OF
BIOCHEMISTRY
2
CURRENT
REVIEWS
OF
THE
BIOCHEMICAL
LITERATURE
3
THE
PRIMARY
BIOCHEMICAL
LITERATURE:
SCIENTIFIC
JOURNALS
4
ACCESS
TO
THE
METHODOLOGICALLY-BASED
BIOCHEMICAL
LITERATURE
6
MONOGRAPHS
AND
SERIES
6
METHODS-BASED
BIOCHEMICAL
JOURNALS
6
REFERENCE
WORKS
AND
HANDBOOKS
7
REFERENCE
WORKS
7
HANDBOOKS
AND
COLLECTED
TABLES
8
LITERATURE
SEARCHES
9
RETROSPECTIVE
LITERATURE
SEARCHES
9
CURRENT
LITERATURE
SEARCHES
9
THE
INTERNET
AS
AN
INFORMATION
RESOURCE
10
DOCUMENTATION
OF
PRACTICAL
WORK
10
THE
LABORATORY
BOOK
10
THE
LAYOUT
OF
THE
LABORATORY
BOOK
11
LITERATURE
11
2
2.1
2.1.1
2.1.2
2.1.3
2.1.4
2.1.5
2.1.6
2.1.7
2.2
2.2.1
GENERAL
LABORATORY
PROCEDURES
13
THE
BIOCHEMISTRY
LABORATORY
13
APPARATUS
NEEDED
IN
EVERY
BIOCHEMISTRY
LABORATORY
13
APPARATUS
THAT
CAN
BE
SHARED
BETWEEN
SEVERAL
LABORATORIES
14
MISCELLANEOUS
SMALL
ITEMS
15
CONTAINERS:
GLASS,
CERAMIC,
METAL
AND
PLASTIC
IN
VARIOUS
SIZES
15
DISPOSABLES
15
SAFETY
EQUIPMENT
16
STANDARD
REAGENTS
16
ROUTINE
BIOCHEMICAL
PROCEDURES
17
SAFETY
REQUIREMENTS
17
X
I
CONTENTS
2.2.2
2.2.3
2.2.4
2.2.5
CLEANING
OF
GLASS
AND
PLASTIC
CONTAINERS
19
WEIGHING
OUT
SOLIDS
20
PIPETTING
AND
MEASURING
LIQUID
VOLUMES
20
PREPARATION
AND
STORAGE
OF
SOLUTIONS:
WATER
QUALITY
AND
THE
PURITY
OF
CHEMICAL
REAGENTS
23
2.2.6
2.2.7
2.2.8
2.2.9
2.2.10
THERMOSTATTING
24
SHAKING
AND
STIRRING
25
USE
OF
PUMPS
26
BUFFERS
27
SUPPLEMENTARY
REAGENTS
(PRESERVATIVES,
CHELATING
AGENTS,
SH
REAGENTS
AND
DETERGENTS)
31
2.2.11
2.2.12
2.3
2.3.1
2.3.2
2.3.3
2.4
PH
DETERMINATION
32
CONDUCTIVITY
MEASUREMENTS
33
WORKING
WITH
RADIOACTIVITY
33
RADIOACTIVE
ISOTOPES
AND
THEIR
DECAY
34
MEASUREMENT
OF
RADIOACTIVITY
36
ALTERNATIVES
TO
RADIOACTIVITY
43
LITERATURE
46
3
3.1
3.1.1
3.1.2
3.1.3
3.2
3.3
3.3.1
3.3.2
3.4
3.4.1
3.4.2
3.4.3
3.5
SAMPLE
PREPARATION
49
CELL
AND
TISSUE
DISRUPTION
49
GENERAL
ASPECTS
OF
PROTEIN
AND
NUCLEIC
ACID
ISOLATION
50
MECHANICAL
HOMOGENISATION
52
NON-MECHANICAL
HOMOGENISATION
PROCEDURES
54
SOLUBILISATION
55
PRECIPITATION
PROCEDURES
FOR
PROTEINS
AND
NUCLEIC
ACIDS
56
PRECIPITATION
OF
PROTEINS
56
PRECIPITATION
OF
NUCLEIC
ACIDS
60
DIALYSIS,
ULTRAFILTRATION
AND
LYOPHILISATION
61
DIALYSIS
61
ULTRAFILTRATION
63
LYOPHILISATION
66
LITERATURE
67
4
4.1
4.1.1
4.1.2
4.1.3
4.1.4
4.2
4.2.1
4.2.2
4.2.3
4.2.4
SEPARATION
METHODS
69
CHROMATOGRAPHY
69
GENERAL
PRINCIPLES
AND
DEFINITIONS
70
COLUMN
CHROMATOGRAPHY
70
PAPER
AND
THIN
LAYER
CHROMATOGRAPHY
102
GAS-LIQUID
CHROMATOGRAPHY
104
ELECTROPHORESIS
106
GENERAL
PRINCIPLES
AND
DEFINITIONS
106
CELLULOSE
ACETATE
ELECTROPHORESIS
109
GEL
ELECTROPHORESIS
110
ISOELECTRIC
FOCUSING
124
CONTENTS
I
XI
4.2.5
4.2.6
4.2.7
4.2.8
4.3
4.3.1
4.3.2
4.3.3
4.3.4
4.4
2D
ELECTROPHORESIS
127
BLOTTING
METHODS
129
EVALUATION
AND
DOCUMENTATION
OF
ELECTROPHORESIS
RESULTS
131
CAPILLARY
ELECTROPHORESIS
131
CENTRIFUGATION
137
BASIC
PRINCIPLES
138
CENTRIFUGES
AND
ROTORS
140
ANALYTICAL
ULTRACENTRIFUGATION
145
PREPARATIVE
CENTRIFUGATION
149
LITERATURE
151
5
5.1
5.1.1
5.1.2
5.1.3
5.1.4
5.1.5
5.1.6
5.1.7
5.1.8
5.1.9
5.1.10
5.1.11
5.2
5.2.1
5.2.2
5.2.3
5.2.4
5.2.5
ANALYTICAL
METHODS
155
PROTEIN
ANALYSIS
155
DETERMINATION
OF
PROTEIN
MOLAR
MASSES
155
QUANTITATION
OF
PROTEINS
157
AMINO
ACID
ANALYSIS
162
END
GROUP
DETERMINATION
163
EDMAN
DEGRADATION
164
PEPTIDE
MAPPING
166
CO
AND
POST-TRANSLATIONAL
MODIFICATION
171
CHEMICAL
MODIFICATION
OF
PROTEINS
173
STRUCTURAL
ANALYSIS
OF
PROTEINS
182
PROTEIN
STABILITY
184
PEPTIDE
SYNTHESIS
AND
IN
VITRO
PROTEIN
SYNTHESIS
187
NUCLEIC
ACID
ANALYSIS
190
DETERMINATION
OF
NUCLEIC
ACID
CONCENTRATION
190
DETERMINATION
OF
NUCLEIC
ACID
SIZE
191
BASE
COMPOSITION
191
RESTRICTION
MAPPING
191
DETECTION
OF
SPECIFIC
DNA
AND
RNA
SEQUENCES
BY
SOUTHERN
AND
N
ORTHEM
BLOTTING
193
5.2.6
DETECTION
OF
SPECIFIC
DNA
AND
RNA
SEQUENCES
BY
THE
POLYMERASE
CHAIN
REACTION
(PCR)
194
5.2.7
5.2.8
5.2.9
5.2.10
5.3
5.3.1
5.3.2
NUCLEIC
ACID
SEQUENCING
199
DETERMINATION
OF
THE
STABILITY
OF
DOUBLE
STRANDED
NUCLEIC
ACIDS
202
OLIGONUCLEOTIDE
SYNTHESIS
204
LABELLING
AND
CHEMICAL
MODIFICATION
OF
NUCLEIC
ACIDS
207
ENZYMATIC
ANALYSIS
208
DIRECT
DETERMINATION
OF
METABOLITE
CONCENTRATIONS
208
DETERMINATION
OF
METABOLITE
CONCENTRATIONS
BY
COUPLED
MEASUREMENTS
209
5.3.3
5.4
DETERMINATION
OF
ENZYME
ACTIVITY
210
LITERATURE
212
XIII
CONTENTS
6
6.1
6.1.1
6.1.2
6.1.3
6.2
6.2.1
6.2.2
6.2.3
6.2.4
6.2.5
6.2.6
6.2.7
6.3
IMMUNOLOGICAL
METHODS
219
ANTIBODIES
219
ANTIBODY
STRUCTURE
220
ANTIBODY
PRODUCTION
221
ANTIBODY
PURIFICATION
224
ANTIBODY-ANTIGEN
INTERACTIONS
225
ANTIBODY-ANTIGEN
INTERACTIONS
IN
SOLUTION
226
ANTIBODY-ANTIGEN
INTERACTIONS
IN
GELS
226
RADIOIMMUNOASSAY
230
ENZYME-LINKED
IMMUNOSORBENT
ASSAY
230
WESTERN
BLOTTING
AND
DOT
BLOTTING
234
IMMUNOFLUORESCENCE
AND
IMMUNOGOLD
ELECTRON
MICROSCOPY
235
FLUORESCENCE
ACTIVATED
CELL
SORTING
235
LITERATURE
236
7
7.1
7.1.1
7.1.2
7.1.3
7.1.4
7.1.5
7.1.6
7.1.7
7.2
7.2.1
7.2.2
7.3
7.3.1
7.3.2
7.3.3
BIOPHYSICAL
METHODS
239
SPECTROSCOPY
239
ABSORPTION
OF
LIGHT
240
SPECTROPHOTOMETERS
243
FLUORESCENCE
248
VIBRATIONAL
SPECTROSCOPY
255
ANISOTROPIC
SPECTROSCOPY
256
NUCLEAR
MAGNETIC
RESONANCE
SPECTROSCOPY
259
MASS
SPECTROMETRY
264
SCATTERING
TECHNIQUES
267
LIGHT-SCATTERING
IN
SOLUTION
267
SCATTERING
WITH
OTHER
RADIATION
273
INTERACTIONS
275
EQUILIBRIUM
DIALYSIS
275
BINDING
STUDIES
USING
FILTRATION
METHODS
276
BINDING
STUDIES
USING
CHROMATOGRAPHY,
ELECTROPHORESIS
AND
CENTRIFUGATION
277
7.3.4
7.3.5
7.3.6
7.3.7
7.4
7.4.1
7.4.2
7.5
BIOMOLECULAR
INTERACTION
ANALYSIS
279
BINDING
STUDIES
USING
PROTECTION
AND
INTERFERENCE
280
CALORIMETRY
281
KINETICS
283
DETERMINATION
OF
STRUCTURE
286
X-RAY
STRUCTURAL
ANALYSIS
286
STRUCTURAL
DATABASES
291
LITERATURE
292
8
8.1
8.1.1
8.1.2
MATHEMATICAL
METHODS
295
STATISTICS
295
OBSERVATIONS
AND
VARIABLES
296
ERRORS
AND
MEAN
VALUES
297
CONTENTS
IXIII
8.1.3
8.2
8.2.1
8.2.2
8.3
8.3.1
8.3.3
8.4
DISTRIBUTIONS
299
QUANTITATIVE
EVALUATION
OF
EXPERIMENTAL
RESULTS
305
ANALYSIS
OF
BINDING
306
ENZYME
KINETICS
307
SEQUENCE
ANALYSIS
313
DATABASES
314
DATABASE
SEARCHING
315
LITERATURE
320
9
9.1
9.1.1
9.1.2
9.1.3
9.1.4
9.1.5
9.1.6
9.1.7
9.1.8
9.1.9
9.1.10
9.1.11
9.2
9.2.1
9.2.2
9.2.3
9.2.4
9.2.5
9.2.6
9.2.7
9.2.8
9.2.9
9.2.10
9.2.11
9.2.12
9.3
QUANTITATIVE
ANALYSIS
OF
BIOCHEMICAL
DATA
321
INTRODUCTION
321
GENERAL
PRINCIPLES
OF
QUANTITATIVE DATA
ANALYSIS
321
EXPERIMENTAL
SYSTEMS
322
MEASUREMENT
AND
SIGNALS
323
MODELS
323
SELECTION
OF
APPROPRIATE
MODELS
324
PARAMETERS
325
ESSENTIAL
STEPS
IN
THE
ANALYSIS
325
FITTING
DATA
BY
THE
METHOD
OF
LEAST
SQUARES
326
GLOBAL
FITTING
OF
MULTIPLE
DATA
SETS
329
INTRODUCTION
TO
ERROR
ESTIMATION
329
INTRODUCTION
TO
NUMERICAL
INTEGRATION
331
APPLICATIONS
332
LINEAR
REGRESSION
332
MICHAELIS-MENTEN
KINETICS
332
DISSOCIATION
KINETICS
334
BINDING
DATA
335
INDEPENDENT
IDENTICAL
BINDING
SITES
336
ANALYSIS
OF
SIMPLE
BINDING
DATA
337
INDEPENDENT
NON-IDENTICAL
BINDING
SITES
337
COOPERATIVE
BINDING
338
ASSOCIATION
KINETICS
339
PRE-STEADY
STATE
KINETICS
340
PH
DEPENDENCE
OF
ENZYME
CATALYSED
REACTIONS
341
ANALYSIS
OF
COMPETITION
EXPERIMENTS
343
GUIDE
TO
THE
COMPACT
DISC
344
APPENDIX
I:
SI-UNITS
345
APPENDIX
II:
CONVERSIONS
INTO
SI-UNITS
346
INDEX
347 |
| any_adam_object | 1 |
| author_GND | (DE-588)124107524 |
| building | Verbundindex |
| bvnumber | BV014686198 |
| callnumber-first | Q - Science |
| callnumber-label | QH345 |
| callnumber-raw | QH345 |
| callnumber-search | QH345 |
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| classification_rvk | WC 4150 |
| classification_tum | CHE 810f |
| ctrlnum | (OCoLC)46601098 (DE-599)BVBBV014686198 |
| dewey-full | 572 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572 |
| dewey-search | 572 |
| dewey-sort | 3572 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Chemie |
| format | Book |
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| genre | (DE-588)4123623-3 Lehrbuch gnd-content |
| genre_facet | Lehrbuch |
| id | DE-604.BV014686198 |
| illustrated | Illustrated |
| indexdate | 2025-04-14T10:01:46Z |
| institution | BVB |
| isbn | 3527302999 |
| language | English German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009960073 |
| oclc_num | 46601098 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-M347 DE-19 DE-BY-UBM DE-29 DE-29T DE-210 DE-20 DE-1046 DE-91 DE-BY-TUM DE-703 DE-526 DE-11 DE-188 |
| owner_facet | DE-355 DE-BY-UBR DE-M347 DE-19 DE-BY-UBM DE-29 DE-29T DE-210 DE-20 DE-1046 DE-91 DE-BY-TUM DE-703 DE-526 DE-11 DE-188 |
| physical | XIII, 360 S. graph. Darst. 1 CD-ROM (12 cm) |
| publishDate | 2002 |
| publishDateSearch | 2002 |
| publishDateSort | 2002 |
| publisher | Wiley-VCH |
| record_format | marc |
| spelling | Biochemical methods a concise guide for students and researchers A. Pingoud ... Weinheim Wiley-VCH 2002 XIII, 360 S. graph. Darst. 1 CD-ROM (12 cm) txt rdacontent n rdamedia nc rdacarrier Überarb. engl. Ausg. von: Pingoud, Alfred: Arbeitsmethoden der Biochemie Biochemistry cabt Biochemical Techniques cabt Biochemie gtt Biochimie - Méthodologie Biyokimya - Yöntembilim Biochemistry Methodology Biochemistry methods Methode (DE-588)4038971-6 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Biochemie (DE-588)4006777-4 gnd rswk-swf (DE-588)4123623-3 Lehrbuch gnd-content Biochemie (DE-588)4006777-4 s Methode (DE-588)4038971-6 s DE-604 Labortechnik (DE-588)4123602-6 s b DE-604 Pingoud, Alfred 1945-2015 Sonstige (DE-588)124107524 oth DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009960073&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Biochemical methods a concise guide for students and researchers Biochemistry cabt Biochemical Techniques cabt Biochemie gtt Biochimie - Méthodologie Biyokimya - Yöntembilim Biochemistry Methodology Biochemistry methods Methode (DE-588)4038971-6 gnd Labortechnik (DE-588)4123602-6 gnd Biochemie (DE-588)4006777-4 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4123602-6 (DE-588)4006777-4 (DE-588)4123623-3 |
| title | Biochemical methods a concise guide for students and researchers |
| title_auth | Biochemical methods a concise guide for students and researchers |
| title_exact_search | Biochemical methods a concise guide for students and researchers |
| title_full | Biochemical methods a concise guide for students and researchers A. Pingoud ... |
| title_fullStr | Biochemical methods a concise guide for students and researchers A. Pingoud ... |
| title_full_unstemmed | Biochemical methods a concise guide for students and researchers A. Pingoud ... |
| title_short | Biochemical methods |
| title_sort | biochemical methods a concise guide for students and researchers |
| title_sub | a concise guide for students and researchers |
| topic | Biochemistry cabt Biochemical Techniques cabt Biochemie gtt Biochimie - Méthodologie Biyokimya - Yöntembilim Biochemistry Methodology Biochemistry methods Methode (DE-588)4038971-6 gnd Labortechnik (DE-588)4123602-6 gnd Biochemie (DE-588)4006777-4 gnd |
| topic_facet | Biochemistry Biochemical Techniques Biochemie Biochimie - Méthodologie Biyokimya - Yöntembilim Biochemistry Methodology Biochemistry methods Methode Labortechnik Lehrbuch |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009960073&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT pingoudalfred biochemicalmethodsaconciseguideforstudentsandresearchers |