Cell growth, differentiation and senescence: a practical approach
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Oxford [u.a.]
Oxford Univ. Press
1999
|
| Schriftenreihe: | The practical approach series
215 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXIV, 308 S. Ill., graph. Darst. |
| ISBN: | 0199637687 0199637695 |
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Datensatz im Suchindex
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|---|---|
| adam_text | CELL GROWTH, DIFFERENTIATION AND SENESCENCE A PRACTICAL APPROACH EDITED
BY GEORGE P. STUDZINSKI DEPARTMENT OF PATHOLOGY AND LABORATORY MEDICINE,
UMD * NEW JERSEY MEDICAL SCHOOL, NEWARK, N.J., USA OXPORD UNIVERSITY
PRESS CONTENTS LIST OF CON TRIB U TORS ABBREVIATIONS 1. SELECTION OF
METHODS FOR MEASURING PROLIFERATION ROBERT WIEDER 1. INTRODUCTION
DEFINING PARAMETERS OF PROLIFERATION 2. CELLS IN TISSUE CULTURE COUNTING
CELL NUMBERS MEASURING DNA CONTENT MEASURING THE RATE OF MITOSIS
MEASURING DNA SYNTHESIS MEASURING ACTIVE METABOLISM AS A REFLECTION OF
VIABLE CELL NUMBER: THE MTT ASSAY MEASURING THE COLLECTIVE CELL VOLUME
3. DETERMINING PROLIFERATION IN VIVO MEASURING DNA SYNTHESIS 4. CELLS
AND TISSUE SAMPLES OBTAINED FROM HUMAN PATIENTS OR ANIMALS DETERMINING
THE MITOTIC INDEX MEASURING CELLULAR DNA CONTENT NUCLEAR ANTIGENS
ASSOCIATED WITH PROLIFERATION: PCNA, KI67, AND AGNOR REFERENCES 1 1 4 4
8 13 15 18 19 20 20 26 27 27 27 30 2. CELL GROWTH AND CYTOTOXICITY
ASSAYS 33 PHILIP SKEHAN 1. INTRODUCTION 33 2. GROWTH AND CYTOTOXICITY
ASSAYS 33 3. QUANTIFYING CELL GROWTH 34 EXPERIMENTAL DESIGN 34 GROWTH
RATE AND DOUBLING TIME 35 POTENCY 35 EFFICACY 36 CONTENTS 4. CELL
CULTURES 36 SEEDING DENSITY 36 DISSOCIATION AND RECOVERY 36 DRUG
SOLUBILIZATION 37 ASSAY DURATION 37 CONTROL WELLS ON EVERY PLATE 37 5.
DYE-BINDING ASSAYS 37 OPTIMIZING AND VALIDATING DYE-BINDING ASSAYS 38
PROTEIN AND BIOMASS STAINS 39 6. METABOLIC IMPAIRMENT ASSAYS 45 NEUTRAL
RED CELL VIABILITY ASSAY 45 MTT REDOX ASSAY 46 ALAMARBLUE (ALB)
OXIDATION-REDUCTION ASSAY 48 CELLULAR ATP ASSAY 51 7. MEMBRANE INTEGRITY
ASSAYS 52 FLUORESCEIN DIACETATE (FDA) 52 8. SURVIVORSHIP ASSAYS 53
ADHERENT VERSUS NON-ADHERENT CELLS 54 LONG-TERM RECOVERY (LTR) ASSAY 54
COLONY-FORMING EFFICIENCY ON TISSUE CULTURE PLASTIC 56 COLONY-FORMING
EFFICIENCY IN SOFT AGAR 57 REFERENCES 59 3. CELL GROWTH AND KINETICS IN
MULTICELL SPHEROIDS 6I RALPH E. DURAND 1. INTRODUCTION 61 2. GROWTH OF
SPHEROIDS 61 OPTIONS 61 EXAMPLE: THE V79 SPHEROID SYSTEM 64 3. SPECIAL
FEATURES OF SPHEROIDS 65 METABOLITE AND CATABOLITE GRADIENTS 65
VIABILITY IN SPHEROID CELL SUBPOPULATIONS 66 4. UNEXPECTED FEATURES OF
SPHEROIDS 66 GENETIC INSTABILITY 66 ANEUPLOIDY*A RESPONSE TO
ARCHITECTURE 68 5. CELL KINETICS IN SPHEROIDS 68 PROBLEMS 68
APPROACHES 68 EXAMPLE: KINETICS IN V79 SPHEROIDS 71 CONTENTS 6. GROWTH
COMPARTMENTS IN SPHEROIDS 72 PROLIFERATION VERSUS QUIESCENCE 72
UNUSUAL GROWTH REGULATION 74 REPOPULATION AFTER CYTOREDUCTION 74 7.
CLINICAL EXTENSIONS: TUMOUR RESISTANCE TO TREATMENT 76 CAN TUMOURS
OUTGROW TREATMENT? 76 IMPLICATIONS AND CLINICAL OPTIONS 77 8.
CONCLUSIONS 78 REFERENCES 78 4. ASSESSMENT OF DNA DAMAGE CELL CYCLE
CHECKPOINTS IN GL AND G2 PHASES OF MAMMALIAN CELLS 8I PATRICK M.
O CONNOR AND JOANY JACKMAN 1. INTRODUCTION 81 2. PROCEDURES FOR
ASSESSMENT OF DNA DAMAGE CHECKPOINTS 82 ASSESSMENT OF THE DNA DAMAGE
CHECKPOINT IN THE GL PHASE OF THE CELL CYCLE 82 ASSESSMENT OF THE DNA
DAMAGE CHECKPOINT IN THE G2 PHASE OF THE CELL CYCLE 88 3. SUMMARY 92
REFERENCES 92 5. ASSESSMENT OF THE ROLE OF GROWTH FACTORS AND THEIR
RECEPTORS IN CELL PROLIFERATION 95 SUBAL BISHAYEE 1. INTRODUCTION 95 2.
MOST GROWTH FACTORS ARE SYNTHESIZED AS TRANSMEMBRANE PROTEINS 95 3.
GROWTH FACTOR RECEPTORS 97 STRUCTURAL FEATURES 97 RECEPTOR ASSAYS 99 4.
CONSEQUENCES OF GROWTH FACTOR*RECEPTOR INTERACTION 105
DIMERIZATION/OLIGOMERIZATION OF THE RECEPTOR 105 INTERACTION BETWEEN
ACTIVATED (PHOSPHORYLATED) RECEPTOR AND SIGNALLING MOLECULES 107 5.
AUTOPHOSPHORYLATION RESULTS IN CONFORMATIONAL CHANGES IN THE RECEPTOR
118 XI CONTENTS 6. GROWTH FACTOR RECEPTORS IN ONCOGENESIS 121 REFERENCES
122 6. ANALYSIS OF GROWTH-SIGNALLING PROTEIN KINASE CASCADES 125
ALESSANDRO ALESSANDRINI 1. INTRODUCTION 125 2. GROWTH FACTORS RESPONSIVE
PATHWAYS 125 THE MEK/ERK PATHWAY 125 THE PHOSPHOINOSITIDE-3 KINASE
PATHWAYS 128 3. TECHNIQUES USED TO ANALYSE PROTEIN KINASES 130
ACTIVATION OF MEK/ERK PATHWAY IN RESPONSE TO GROWTH FACTORS 130 IS MY
PROTEIN AN ERK SUBSTRATE? 134 4. DETERMINING THE PHYSIOLOGICAL
SIGNIFICANCE OF A PROTEIN PHOSPHORYLATED BY A SPECIFIC KINASE: THE USE
OF SPECIFIC TECHNIQUES 136 EXPRESSION IN MAMMALIAN CELLS 137 IN VIVO 32
P-LABELLING OF CULTURE CELLS AND TWO-DIMENSIONAL TRYPTIC PHOSPHOPEPTIDE
ANALYSIS 137 5. CONCLUDING REMARKS 142 REFERENCES 143 7. CYTOMETRIC
ANALYSIS OF THE PRB PATHWAY 145 GLORIA JUAN, FRANK TRAGANOS, AND
ZBIGNIEW DARZYNKIEWICZ 1. INTRODUCTION 145 2. CYCLINS D, E, A, AND BL
AND CDK INHIBITORS (CKIS) 146 3. EXPRESSION OF CYCLINS D, E, A, AND BL
AND CKIS DETECTED BY FLOW CYTOMETRY 151 4. CRITICAL STEPS IN DETECTION
OF CYCLINS OR CKIS 153 5. DETECTION OF PRB PHOSPHORYLATION 154
REFERENCES 158 8. METHODS FOR ONCOGENE DETECTION I6I STEPHEN C. COSENZA,
STACEY J. BAKER, AND E. PREMKUMAR REDDY 1. INTRODUCTION 161 XII CONTENTS
2. DETECTION OF ONCOGENES USING NIH-3T3 CELL FOCUS FORMATION ASSAYS 162
3. TRANSFECTION USING CELLULAR GENOMIC DNA 163 4. ISOLATION OF HIGH
MOLECULAR WEIGHT GENOMIC DNA FROM TUMOUR SAMPLES AND TISSUE CULTURE
CELLS AND TRANSFECTION INTO NIH-3T3 CELLS USING CALCIUM PHOSPHATE
PRECIPITATION 164 5. TRANSFECTION OF NIH-3T3 CELLS BY CALCIUM PHOSPHATE
PRECIPITATION USING PLASMID DNA (3,12) 166 6. NIH-3T3
CO-TRANSFECTION/NUDE MOUSE TUMORIGENICITY ASSAY 167 7. SECONDARY NIH-3T3
FOCI AND TUMOUR INDUCTION ASSAYS 172 8. SOFT AGAR ASSAYS 172 9. SUMMARY
174 REFERENCES 175 9. ISOLATION AND VISUALIZATION OF THE PRINCIPAL
COMPONENTS OF NUCLEAR ARCHITECTURE 177 GARY S. STEIN, MARTIN MONTECINO,
SANDRA MCNEIL, SHIRWIN POCKWINSE, ANDRE J. VAN WIJNEN, JANET L. STEIN,
AND JANE B. LIAN 1. INTRODUCTION 177 2. IN VITRO EXPERIMENTAL APPROACHES
178 CHROMATIN ORGANIZATION 178 ISOLATION AND CHARACTERIZATION OF NUCLEAR
MATRIX COMPONENTS 186 3. IN VIVO EXPERIMENTAL APPROACHES 189 SUBCELLULAR
FRACTIONATION OF COMPONENTS OF NUCLEAR ARCHITECTURE FOR IN SITU
IMMUNOFLUORESCENCE ANALYSIS 189 IN VIVO EXPRESSION OF TRANSCRIPTION
FACTORS 196 EPITOPE TAGS 200 VISUALIZATION OF THE COMPONENTS OF
TRANSCRIPTION AND ASSESSMENT OF ACTIVITY 201 4. INSTRUMENTATION FOR IN
SITU ANALYSIS 203 DIRECT IMMUNOFLUORESCENCE 203 DIGITAL IMAGING 204
REFERENCES 206 10. ANTISENSE OLIGONUCLEOTIDES: TOOLS FOR ELUCIDATING
GENE FUNCTION 209 JOHN J. WOLF AND W. MICHAEL FLANAGAN 1. INTRODUCTION
209 XIII CONTENTS 2. CHEMICAL MODIFICATIONS OF ANTISENSE ONS 210 3.
ANTISENSE ONS AS BIOLOGICAL TOOLS FOR ELUCIDATING GENE FUNCTION 212
SELECTION OF ANTISENSE ONS 212 GUIDELINES FOR SELECTING ANTISENSE ONS
213 SEQUENCE-DEPENDENT NON-ANTISENSE EFFECTS 214 GUIDELINES FOR
CONDUCTING ANTISENSE EXPERIMENTS 215 ANTISENSE TARGETS 216 4. ON
DELIVERY 216 ON DELIVERY USING CATIONIC LIPIDS 217 COMMENTS ABOUT GS3815
CYTOFECTIN 220 ELECTROPORATION OF ONS INTO CELLS 220 5. CONCLUSIONS 222
REFERENCES 223 11. HUMAN LEUKAEMIA CELLS AS A MODEL DIFFERENTIATION
SYSTEM 225 DOROTHY C. MOORE AND GEORGE P. STUDZINSKI 1. INTRODUCTION 225
2. CELL TYPES 225 3. ASSESSMENT OF MONOCYTIC DIFFERENTIATION 226 4.
ASSESSMENT OF ERYTHROID DIFFERENTIATION 230 5. ASSESSMENT OF
MEGAKARYOCYTIC DIFFERENTIATION 236 6. SUMMARY . 239 REFERENCES 239 12.
INDUCTION OF DIFFERENTIATION OF HUMAN INTESTINAL CELLS IN VITRO 241
HEIDE S. CROSS AND ENIKO KALLAY 1. INTRODUCTION 241 2. DIFFERENTIATION
AGENTS 242 VITAMIN D COMPOUNDS 242 HIGH LEVELS OF CALCIUM (1.8-2.4 MM)
IN THE CULTURE MEDIUM 243 3. MODEL SYSTEMS: ESTABLISHMENT AND USE 243
CELL LINES (CACO-2) 243 PRIMARY CULTURES 244 FRESHLY ISOLATED CELLS 245
FROZEN HUMAN TISSUE 247 XIV CONTENTS 4. MARKERS OF DIFFERENTIATION AND
MARKERS OF CESSATION OF PROLIFERATION 247 MORPHOLOGY AND RELATED MARKERS
247 ENZYMATIC ACTIVITIES 249 EVALUATION OF PROLIFERATION 251 CELL
CYCLE-ASSOCIATED MARKERS 253 MARKERS OCCURRING DURING COLON CANCER
PROGRESSION 257 E-CADHERIN EXPRESSION 258 REFERENCES 260 13.
PROLIFERATION AND DIFFERENTIATION OF HUMAN PROSTATE CELLS 263 THERESE
THALHAMMER AND HEIDE S. CROSS 1. INTRODUCTION 263 COMPONENTS OF PROSTATE
TISSUE 264 2. IN VITRO MODELS 264 HUMAN PROSTATE CANCER CELL LINES:
(LNCAP, PC3, AND DU-145) 265 PRIMARY CULTURE FROM HUMAN PROSTATE 266 3.
VIABILITY, METABOLIC ACTIVITY, AND PROLIFERATION 266 4. MEASUREMENT OF
PROSTATE-SPECIFIC ANTIGEN (PSA) 268 5. SIGNAL TRANSDUCTION 269 PROTEIN
KINASE C (PKC) 269 PROTEIN TYROSINE KINASES 273 MEASUREMENT OF [CA 2+ ]J
IN SINGLE CELLS 273 GROWTH FACTORS IN THE PROSTATE 274 WESTERN BLOT
ANALYSIS OF AMPHIREGULIN AND BFGF 277 6. SUMMARY 278 REFERENCES 279 14.
SENESCENCE AND IMMORTALIZATION OF HUMAN CELLS 281 KAREN HUBBARD AND
HARVEY L. OZER 1. INTRODUCTION 281 2. CELLULAR REPLICATIVE SENESCENCE
282 3. SV40 TRANSFORMATION AND CRISIS 287 41 SV40-IMMORTALIZED CELL
LINES 290 5. TELOMERES AND TELOMERASE 293 6. CONDITIONAL SV40
TRANSFORMANTS 296 XV CONTENTS 7. OTHER APPROACHES TO IMMORTALIZATION OF
HUMAN CELLS 297 8. SUMMARY 298 REFERENCES 299 LIST OF SUPPLIERS 301
INDEX 305 XVI
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| spelling | Cell growth, differentiation and senescence a practical approach ed. by George P. Studzinski Oxford [u.a.] Oxford Univ. Press 1999 XXIV, 308 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier The practical approach series 215 Cell Death Laboratory Manuals Cell Differentiation Laboratory Manuals Cell Division Laboratory Manuals Cell death Laboratory manuals Cell differentiation Laboratory manuals Cells Growth Research Laboratory manuals Zelltod (DE-588)4207415-0 gnd rswk-swf Zellwachstum (DE-588)4190674-3 gnd rswk-swf Analyse (DE-588)4122795-5 gnd rswk-swf Zellwachstum (DE-588)4190674-3 s Analyse (DE-588)4122795-5 s DE-604 Zelltod (DE-588)4207415-0 s Studzinski, George P. Sonstige oth The practical approach series 215 (DE-604)BV007921321 215 GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009930865&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cell growth, differentiation and senescence a practical approach The practical approach series Cell Death Laboratory Manuals Cell Differentiation Laboratory Manuals Cell Division Laboratory Manuals Cell death Laboratory manuals Cell differentiation Laboratory manuals Cells Growth Research Laboratory manuals Zelltod (DE-588)4207415-0 gnd Zellwachstum (DE-588)4190674-3 gnd Analyse (DE-588)4122795-5 gnd |
| subject_GND | (DE-588)4207415-0 (DE-588)4190674-3 (DE-588)4122795-5 |
| title | Cell growth, differentiation and senescence a practical approach |
| title_auth | Cell growth, differentiation and senescence a practical approach |
| title_exact_search | Cell growth, differentiation and senescence a practical approach |
| title_full | Cell growth, differentiation and senescence a practical approach ed. by George P. Studzinski |
| title_fullStr | Cell growth, differentiation and senescence a practical approach ed. by George P. Studzinski |
| title_full_unstemmed | Cell growth, differentiation and senescence a practical approach ed. by George P. Studzinski |
| title_short | Cell growth, differentiation and senescence |
| title_sort | cell growth differentiation and senescence a practical approach |
| title_sub | a practical approach |
| topic | Cell Death Laboratory Manuals Cell Differentiation Laboratory Manuals Cell Division Laboratory Manuals Cell death Laboratory manuals Cell differentiation Laboratory manuals Cells Growth Research Laboratory manuals Zelltod (DE-588)4207415-0 gnd Zellwachstum (DE-588)4190674-3 gnd Analyse (DE-588)4122795-5 gnd |
| topic_facet | Cell Death Laboratory Manuals Cell Differentiation Laboratory Manuals Cell Division Laboratory Manuals Cell death Laboratory manuals Cell differentiation Laboratory manuals Cells Growth Research Laboratory manuals Zelltod Zellwachstum Analyse |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009930865&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV007921321 |
| work_keys_str_mv | AT studzinskigeorgep cellgrowthdifferentiationandsenescenceapracticalapproach |