Cystic fibrosis methods and protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, N.J.
Humana Press
2002
|
| Schriftenreihe: | Methods in molecular medicine
70 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XVI, 631 S. Ill., graph. Darst. |
| ISBN: | 0896038971 |
Internformat
MARC
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| 245 | 1 | 0 | |a Cystic fibrosis methods and protocols |c ed. by William R. Skach |
| 264 | 1 | |a Totowa, N.J. |b Humana Press |c 2002 | |
| 300 | |a XVI, 631 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular medicine |v 70 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 7 | |a Kystische fibrose |2 gtt | |
| 650 | 4 | |a Mucoviscidose - Manuels de laboratoire | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a Cystic Fibrosis Transmembrane Conductance Regulator |v Laboratory Manuals | |
| 650 | 4 | |a Cystic Fibrosis |x genetics |v Laboratory Manuals | |
| 650 | 4 | |a Cystic fibrosis |v Laboratory manuals | |
| 650 | 0 | 7 | |a Mukoviszidose |0 (DE-588)4040669-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Molekulare Medizin |0 (DE-588)4543844-4 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Mukoviszidose |0 (DE-588)4040669-6 |D s |
| 689 | 0 | 1 | |a Molekulare Medizin |0 (DE-588)4543844-4 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Skach, William R. |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in molecular medicine |v 70 |w (DE-604)BV011334960 |9 70 | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-009890253 | ||
Datensatz im Suchindex
| _version_ | 1804129323341316096 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
Part I Genetics of Cystic Fibrosis
1 CFTR Mutation Detection by Multiplex Heteroduplex (mHET)
Analysis on MDE Gel
Julian Zielenski, Isabel Aznarez, Tuncer Onay,
John Tzounzouris, Danuta Markiewicz, and Lap Chee Tsui 3
2 cDNA Microarrays for Pharmacogenomic Analysis of Cystic Fibrosis
Meera Srivastava, Ofer Eidelman, and Harvey B. Pollard 21
3 Natural Animal Models of Human Genetic Diseases
Jeffrey J. Wine, Michael Dean, and Damjan Glavac 31
Part II CFTR Structure and Function:
Structure, Gating, and Regulation
4 Electrophysiological Approach to Studying CFTR
Horst Fischer 49
5 Quantitative Analysis of ATP Dependent Gating of CFTR
Allan Powe, Zhen Zhou, Tzyh Chang Hwang, and Georg Nagel ...67
6 CFTR Regulation by Phosphorylation
Tang Zhu, Deborah A. R. Hinkson, David Dahan,
Alexandra Evagelidis, and John W. Hanrahan 99
7 Transepithelial Measurements of Bicarbonate Secretion
in Calu 3 Cells
Robert J. Bridges 111
8 Transepithelial Impedance Analysis of Chloride Secretion
Ashvani K. Singh, Sangeeta Singh, Daniel C. Devor,
Raymond A. Frizzell, Willy van Driessche,
and Robert J. Bridges 129
9 Studies of the Molecular Basis for Cystic Fibrosis
Using Purified Reconstituted CFTR Protein
liana Kogan, Mohabir Ramjeesingh, Canhui Li,
and Christine E. Bear 143
vii
10 Probing CFTR Channel Structure and Function
Using the Substituted Cysteine Accessibility Method
Myles H. Akabas 159
11 Methods for the Study of intermolecular and Intramolecular
Interactions Regulating CFTR Function
Anjaparavanda P. Naren 175
12 Fluorescent Indicator Methods
to Assay Functional CFTR Expression in Cells
Alan S. Verkman and Sujatha Jayaraman 187
Expression, Folding, and Degradation
13 Immunolocalization of CFTR in Intact Tissue and Cultured Cells
Christopher R. Marino 199
14 Analysis of CFTR Trafficking and Polarization
Using Green Fluorescent Protein and Confocal Microscopy
Bryan D. Moyer and Bruce A. Stanton 217
15 CFTR Folding and Maturation in Cells
Mohamed Benharouga, Manu Sharma, and Gergely L. Lukacs 229
16 Isolation of CFTR:
Chaperone Complexes by Co lmmunoprecipitation
Geoffrey C. Meacham and Douglas M. Cyr 245
17 CFTR Expression and ER Associated Degradation in Yeast
Yimao Zhang, Susan Michaelis, and Jeffrey L. Brodsky 257
18 Manipulating the Folding Pathway of AF508 CFTR
Using Chemical Chaperones
Marybeth Howard and William J. Welch 267
19 CFTR Degradation and Aggregation
Michael J. Corboy, Philip J. Thomas, and W. Christian Wigley.. 277
20 In Vitro Reconstitution of CFTR Biogenesis and Degradation
Jon Oberdorf and William R. Skach 295
21 In Vitro CFTR Folding Assays
Rhesa D. Stidham, W. Christian Wigley, and Philip J. Thomas... 311
22 Analysis of CFTR Endocytosis by Cell Surface Biotinylation
Kelly Weixel and Neil A. Bradbury 323
Regulatory Complexes
23 CFTR Regulation of ENaC
Scott H. Donaldson, Elaine G. Poligone, and M. Jackson Stutts.... 343
24 Yeast Two Hybrid Identification and Analysis of Protein Interactions
with CFTR
Viswanathan Raghuram, Kenneth R. Hallows,
and J. Kevin Foskett 365
25 Biochemical Assays for Studying Indirect Interactions
Between CFTR and the Cytoskeleton
Peter J. Mohler, Patricia L. Kultgen, M. Jackson Stutts,
and Sharon L. Milgram 383
26 CFTR Associated ATP Transport and Release
Marie E. Egan 395
Part III Pathophysiology of Cystic Fibrosis
27 Inflammatory Mediators in CF Patients
Jay B. Hilliard, Michael W. Konstan, and Pamela B. Davis 409
28 Bacterial Colonization and Infection in the CF Lung
Scott D. Sagel, Elaine B. Dowell, and Frank J. Accurso 433
29 Antimicrobial Peptides and Proteins in the CF Airway
Alexander M. Cole and Tomas Gam 447
30 Bacterial Epithelial Interactions
Ruth Bryan and Alice Prince 465
31 Thin Film Measurements
of Airway Surface Liquid Volume/Composition
and Mucus Transport Rates In Vitro
Robert Tarran and Richard C. Boucher 479
Animal Models of Cystic Fibrosis
32 Murine Models of CF Airway Infection and Inflammation
James F. Chmiel, Michael W. Konstan, and Melvin Berger 495
33 Analysis of Lipid Abnormalities in CF Mice
Steven D. Freedman, Paola G. Blanco, Julie C. Shea,
and Juan G. Alvarez 517
34 Bioelectric Measurement of CFTR Function in Mice
Barbara R. Grubb 525
35 Xenograft Model of the CF Airway
Mohammed Filali, Yulong Zhang, Teresa C. Ritchie,
and John F. Engelhardt 537
36 Development of Conditionally Immortalized Epithelial Cell Lines
from CF and Non CF Mice
Calvin U. Cotton 551
37 Technical Approaches to Analyze the In Vivo Ion Composition
of Airway Surface Liquid
Jean Marie Zahm, Sonia Baconnais, Gerard Balossier,
and Edith Puchelle 563
Part IV Novel Therapeutic Approaches for Cystic Fibrosis
38 Design of Gene Therapy Clinical Trials in CF Patients
Kimberly V. Curlee and Eric J. Sorscher 575
39 Formulation of Synthetic Vectors for Cystic Fibrosis Gene Therapy
John Marshall and Seng H. Cheng 585
40 Adeno Associated Viral Vectors for CF Gene Therapy
Terence R. Flotte, Isabel Virella Lowell, and Kye A. Chesnut 599
Index 609
|
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| id | DE-604.BV014534232 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T19:03:19Z |
| institution | BVB |
| isbn | 0896038971 |
| language | English |
| lccn | 2001039467 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009890253 |
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| physical | XVI, 631 S. Ill., graph. Darst. |
| publishDate | 2002 |
| publishDateSearch | 2002 |
| publishDateSort | 2002 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular medicine |
| series2 | Methods in molecular medicine |
| spelling | Cystic fibrosis methods and protocols ed. by William R. Skach Totowa, N.J. Humana Press 2002 XVI, 631 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular medicine 70 Includes bibliographical references and index Kystische fibrose gtt Mucoviscidose - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Cystic Fibrosis Transmembrane Conductance Regulator Laboratory Manuals Cystic Fibrosis genetics Laboratory Manuals Cystic fibrosis Laboratory manuals Mukoviszidose (DE-588)4040669-6 gnd rswk-swf Molekulare Medizin (DE-588)4543844-4 gnd rswk-swf Mukoviszidose (DE-588)4040669-6 s Molekulare Medizin (DE-588)4543844-4 s DE-604 Skach, William R. Sonstige oth Methods in molecular medicine 70 (DE-604)BV011334960 70 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009890253&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cystic fibrosis methods and protocols Methods in molecular medicine Kystische fibrose gtt Mucoviscidose - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Cystic Fibrosis Transmembrane Conductance Regulator Laboratory Manuals Cystic Fibrosis genetics Laboratory Manuals Cystic fibrosis Laboratory manuals Mukoviszidose (DE-588)4040669-6 gnd Molekulare Medizin (DE-588)4543844-4 gnd |
| subject_GND | (DE-588)4040669-6 (DE-588)4543844-4 |
| title | Cystic fibrosis methods and protocols |
| title_auth | Cystic fibrosis methods and protocols |
| title_exact_search | Cystic fibrosis methods and protocols |
| title_full | Cystic fibrosis methods and protocols ed. by William R. Skach |
| title_fullStr | Cystic fibrosis methods and protocols ed. by William R. Skach |
| title_full_unstemmed | Cystic fibrosis methods and protocols ed. by William R. Skach |
| title_short | Cystic fibrosis methods and protocols |
| title_sort | cystic fibrosis methods and protocols |
| topic | Kystische fibrose gtt Mucoviscidose - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Cystic Fibrosis Transmembrane Conductance Regulator Laboratory Manuals Cystic Fibrosis genetics Laboratory Manuals Cystic fibrosis Laboratory manuals Mukoviszidose (DE-588)4040669-6 gnd Molekulare Medizin (DE-588)4543844-4 gnd |
| topic_facet | Kystische fibrose Mucoviscidose - Manuels de laboratoire Wetenschappelijke technieken Wissenschaftliches Arbeiten Cystic Fibrosis Transmembrane Conductance Regulator Laboratory Manuals Cystic Fibrosis genetics Laboratory Manuals Cystic fibrosis Laboratory manuals Mukoviszidose Molekulare Medizin |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009890253&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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| work_keys_str_mv | AT skachwilliamr cysticfibrosismethodsandprotocols |