GTPase protocols: the Ras superfamily
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, N.J.
Humana Press
2002
|
| Schriftenreihe: | Methods in molecular biology
189 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XI, 267 S. Ill., graph. Darst. |
| ISBN: | 089603934X |
Internformat
MARC
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| 264 | 1 | |a Totowa, N.J. |b Humana Press |c 2002 | |
| 300 | |a XI, 267 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 189 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 7 | |a Biofísica |2 larpcal | |
| 650 | 4 | |a GTPase - Manuels de laboratoire | |
| 650 | 7 | |a GTPasen |2 gtt | |
| 650 | 4 | |a Protéines ras - Manuels de laboratoire | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a GTP-Binding Proteins |v Laboratory Manuals | |
| 650 | 4 | |a Gene Expression Regulation |v Laboratory Manuals | |
| 650 | 4 | |a Guanosine triphosphatase |v Laboratory manuals | |
| 650 | 4 | |a Ras proteins |v Laboratory manuals | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-009856455 | ||
Datensatz im Suchindex
| _version_ | 1804129269449752576 |
|---|---|
| adam_text | Contents
Preface v
Contributors ix
Part I. General Protocols
1 The GTPase Cycle: How Dominant Inhibitory Mutants Block
the Biological Functions of Small GTPases
Edward J. Manser 3
2 Preparation of GTPases for Structural and Biophysical Analysis
Susan J. M. Smith and Katrin Rittinger 13
3 Using cDNA Representational Difference Analysis
(cDNA RDA) in Combination with Microarrays to Identify
Rac Regulated Genes
Arndt A. P. Schmitz, Robert Lucito, and Linda Van Aelst 25
4 Fluorescence Methods in the Study of Small GTP Binding Proteins
Mohammad Reza Ahmadian, Alfred Wittinghofer,
and Christian Herrmann 45
Part II. Specific Methods:
Ras Family GTPases
5 Ras and Rac as Activators of Reactive Oxygen Species (ROS)
Herbert Archer and Dafna Bar Sagi 67
6 Interfering with Ras Signaling Using Membrane Permeable
Peptides or Drugs
Hiroshi Maruta, Hong He, and Thao Nheu 75
7 A Ras Based Module to Generate 32P Labeled Fusion Proteins
for Blot Overlays
Zhoushen Zhao and Louis Lim 87
Rho Family GTPases
8 Determination of the Activity of Rho Like GTPases in Cells
Angeliki Malliri, Jean P. ten Klooster, Christina Olivio,
and John G. Collard 99
9 Introduction of Dominant Inhibitory Proteins Directed Against ROK
and MRCK Kinases
Ivan Tan, Louis Lim, and Thomas Leung 111
vii
viii Contents
10 Effects of Rho Family GTPases on Cell Cell Adhesion
Masaki Fukata, Masato Nakagawa, Shinya Kuroda,
and Kozo Kaibuchi 121
11 Cell Motility and Invasion Assays
Jackie Banyard and Marc Symons 129
Rab Family GTPases
12 Isolation of Regulatory Proteins for the Rab3 Subfamily GTPases
Hiroyuki Nakanishi, Takuya Sasaki, and Yoshimi Takai 143
13 Analysis and Preparation of Stable Complexes between
Rab GTPases, Rab Escort Protein, and Rab Geranylgeranyl
Transferase
Jose B. Pereira Leal, Anita Q. Gomes,
and Miguel C. Seabra 157
Arf Family GTPases
14 Preparation of Myristoylated Arf1 and Arf6 Proteins
Paul A. Randazzo and Henry M. Fales 169
15 ARF Directed Guanine Nucleotide Exchange (GEP) Proteins
Gustavo Pacheco Rodriguez, Joel Moss,
and Martha Vaughan 181
16 Arf6 and its Role in Cytoskeletal Modulation
Julie G. Donaldson 191
17 ARF GTPase Activating Protein 1
Irit Huber, Edna Cukierman, Miriam Rotman,
and Dan Cassel 199
Ran GTPases
18 The Use of Permeabilized Cell Systems to Study Nuclear Transport
Amy M. Brownawell, James M. Holaska, Ian G. Macara,
and Bryce M. Paschal 209
19 Analysis of Nuclear Protein Import and Export In Vitro Using
Fluorescent Cargoes
Ralph H. Kehlenbach and Larry Gerace 231
20 Characterization of the Effects of RanGTP on the Microtubule
Cytoskeleton
Sofia B. Lizarraga, Yixian Zheng, and Andrew R. Wilde 247
Index 261
|
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| dewey-search | 612/.01575 |
| dewey-sort | 3612 41575 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Medizin |
| format | Book |
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| id | DE-604.BV014411320 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T19:02:27Z |
| institution | BVB |
| isbn | 089603934X |
| language | English |
| lccn | 2002022973 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009856455 |
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| physical | XI, 267 S. Ill., graph. Darst. |
| publishDate | 2002 |
| publishDateSearch | 2002 |
| publishDateSort | 2002 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | GTPase protocols the Ras superfamily edited by Ed Manser ... Totowa, N.J. Humana Press 2002 XI, 267 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 189 Includes bibliographical references and index Biofísica larpcal GTPase - Manuels de laboratoire GTPasen gtt Protéines ras - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten GTP-Binding Proteins Laboratory Manuals Gene Expression Regulation Laboratory Manuals Guanosine triphosphatase Laboratory manuals Ras proteins Laboratory manuals Guanosintriphosphatasen (DE-588)4324625-4 gnd rswk-swf Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf Ras-Proteine (DE-588)4323989-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Ras-Proteine (DE-588)4323989-4 s Biochemische Analyse (DE-588)4255721-5 s DE-604 Guanosintriphosphatasen (DE-588)4324625-4 s Manser, Ed Sonstige oth Methods in molecular biology 189 (DE-604)BV035362695 189 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009856455&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | GTPase protocols the Ras superfamily Methods in molecular biology Biofísica larpcal GTPase - Manuels de laboratoire GTPasen gtt Protéines ras - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten GTP-Binding Proteins Laboratory Manuals Gene Expression Regulation Laboratory Manuals Guanosine triphosphatase Laboratory manuals Ras proteins Laboratory manuals Guanosintriphosphatasen (DE-588)4324625-4 gnd Biochemische Analyse (DE-588)4255721-5 gnd Ras-Proteine (DE-588)4323989-4 gnd |
| subject_GND | (DE-588)4324625-4 (DE-588)4255721-5 (DE-588)4323989-4 (DE-588)4143413-4 |
| title | GTPase protocols the Ras superfamily |
| title_auth | GTPase protocols the Ras superfamily |
| title_exact_search | GTPase protocols the Ras superfamily |
| title_full | GTPase protocols the Ras superfamily edited by Ed Manser ... |
| title_fullStr | GTPase protocols the Ras superfamily edited by Ed Manser ... |
| title_full_unstemmed | GTPase protocols the Ras superfamily edited by Ed Manser ... |
| title_short | GTPase protocols |
| title_sort | gtpase protocols the ras superfamily |
| title_sub | the Ras superfamily |
| topic | Biofísica larpcal GTPase - Manuels de laboratoire GTPasen gtt Protéines ras - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten GTP-Binding Proteins Laboratory Manuals Gene Expression Regulation Laboratory Manuals Guanosine triphosphatase Laboratory manuals Ras proteins Laboratory manuals Guanosintriphosphatasen (DE-588)4324625-4 gnd Biochemische Analyse (DE-588)4255721-5 gnd Ras-Proteine (DE-588)4323989-4 gnd |
| topic_facet | Biofísica GTPase - Manuels de laboratoire GTPasen Protéines ras - Manuels de laboratoire Wetenschappelijke technieken Wissenschaftliches Arbeiten GTP-Binding Proteins Laboratory Manuals Gene Expression Regulation Laboratory Manuals Guanosine triphosphatase Laboratory manuals Ras proteins Laboratory manuals Guanosintriphosphatasen Biochemische Analyse Ras-Proteine Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009856455&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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| work_keys_str_mv | AT mansered gtpaseprotocolstherassuperfamily |