Holdings: Fluorescence spectroscopy, imaging and probes

Fluorescence spectroscopy, imaging and probes: new tools in chemical, physical and life sciences

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Bibliographic Details
Format:	Book
Language:	English
Published:	Berlin [u.a.] Springer 2002
Series:	Springer series on fluorescence 2
Subjects:	Fluorescence spectroscopy > Congresses Fluoreszenzspektroskopie Konferenzschrift > 2001 > Amsterdam
Online Access:	Inhaltsverzeichnis
Physical Description:	XXV, 390 S. Ill., graph. Darst.
ISBN:	3540427686

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adam_text	CONTENTS PART 1: FLUORESCENCE SPECTROSCOPY: NEW APPROACHES AND PROBES 1 ADVANCED LUMINESCENT LABELS, PROBES AND BEADS AND THEIR APPLICATION TO LUMINESCENCE BIOASSAY AND IMAGING................................... 3 O. S. W OLFBEIS , M. B OEHMER , A. D UERKOP , J. E NDERLEIN , M. G RUBER , I. K LIMANT , C. K RAUSE , J. K UERNER , G. L IEBSCH , Z. L IN , B. O SWALD , AND M. W U 1.1 INTRODUCTION.............................................................................................. 4 1.2 A GENERAL LOGIC FOR DESIGNING FLUORESCENT CYANINE TYPE PROBES AND LABELS OF DEFINED COLOR.................................................................... 6 1.3 DIODE LASER-EXCITABLE PROBES FOR GENERAL PROTEIN DETECTION................... 8 1.4 DIODE LASER-COMPATIBLE AMINO-REACTIVE COVALENT LABELS................... 10 1.5 DIODE LASER-ASSISTED FLUORESCENT SINGLE MOLECULE DETECTION OF DYES AND LABELED PROTEINS................................................................. 11 1.6 NEW LABELS FOR FLOW CYTOMETRIC DETERMINATION OF HSA...................... 14 1.7 DIODE LASER-EXCITABLE DNA LABELS ....................................................... 15 1.8 NEW RESONANCE ENERGY TRANSFER GENE ASSAYS...................................... 16 1.9 REACTIVE RUTHENIUM LIGAND COMPLEXES AS MARKERS FOR BIOASSAYS....... 17 1.10 DIODE LASER-EXCITABLE FLUORESCENT POLYMER BEADS ............................... 18 1.11 POLYANILINE-COATED NANO-BEADS (~200 NM IN DIAMETER) WITH PH DEPENDENT FLUORESCENCE ......................................................................... 20 1.12 PHOSPHORESCENT POLY(ACRYLONITRILE) NANOSPHERES (10-100 NM IN YY ) AS MARKERS FOR OPTICAL ASSAYS................................................................ 22 1.13 COMPETITIVE BINDING OF STREPTAVIDIN TO BIOTINYLATED NANOBEADS AS STUDIED BY RESONANCE ENERGY TRANSFER............................................. 25 1.14 NANOBEADS AS REFERENCE DYES IN LUMINESCENT LIFETIME IMAGING USING DLR............................................................................................. 27 1.15 PHOSPHORESCENT NANOSPHERES FOR USE IN ADVANCED TIME-RESOLVED MULTIPLEXED BIOASSAYS ( YY , YY ).................................................................. 30 1.16 BEADS DYED WITH A EUROPIUM-BASED LABEL AND EXCITABLE WITH THE 405-NM LED DIODE LASER ............................................................... 34 1.17 A EUROPIUM(III)-BASED PROBE FOR USE IN OXIDASE-ASSOCIATED REACTIONS................................................................................................ 35 1.17.1 SIGNIFICANCE OF PROBES FOR HYDROGEN PEROXIDE (HP).................. 35 1.17.2 A NEW PROBE FOR HYDROGEN PEROXIDE......................................... 35 1.17.3 GLUCOSE ASSAY USING THE EUROPIUM PROBE ................................ 36 1.17.4 PEROXIDASE ASSAY USING THE EUROPIUM PROBE............................. 37 1.17.5 CATALASE....................................................................................... 38 1.17.6 A NEW ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)............ 38 REFERENCES....................................................................................................... 40 CONTENTS X 2 FLUORESCENCE SPECTRAL ENGINEERING - BIOPHYSICAL AND BIOMEDICAL APPLICATIONS................................................................................................... 43 J. R. L AKOWICZ , I. G RYCZYNSKI , Y. S HEN , J. M ALICKA , S. D A URIA , AND Z. G RYCZYNSKI 2.1 INTRODUCTION............................................................................................ 44 2.1.1 FLUORESCENCE SPECTRAL ENGINEERING............................................. 45 2.1.2 OVERVIEW OF METALLIC SURFACE EFFECTS ON FLUORESCENCE.............. 47 2.1.3 THEORY FOR FLUOROPHORE METAL INTERACTIONS................................. 50 2.1.4 SPATIAL DISTRIBUTION OF EMISSION NEAR METAL SURFACES ............... 54 2.1.5 RESONANCE ENERGY TRANSFER........................................................ 55 2.2 EXPERIMENTAL RESULTS ON FLUOROPHORE-METAL INTERACTIONS ..................... 56 2.2.1 SILVER ISLAND FILMS AND EXPERIMENTAL GEOMETRY........................ 56 2.2.2 EFFECTS OF SILVER ISLAND FILMS ON EMISSION SPECTRA OF RHODAMINE B AND ROSE BENGAL................................................... 56 2.2.3 EFFECT OF SILVER ISLAND FILMS ON PHOTOSTABILITY........................... 58 2.2.4 EFFECTS OF SILVER ISLAND FILMS ON THE LIFETIME OF RHODAMINE B AND ROSE BENGAL...................................................................... 59 2.2.5 EFFECT OF QUANTUM YIELD ON SILVER ISLAND ENHANCEMENTS........... 60 2.2.6 EFFECTS OF SILVER ISLANDS ON INTRINSIC PROTEIN FLUORESCENCE......... 61 2.2.7 EFFECTS OF SILVER ISLANDS ON NUCLEIC ACID BASES AND DNA......... 62 2.2.8 EFFECTS OF SILVER ISLANDS ON RESONANCE ENERGY TRANSFER............ 63 2.3 CONCLUSION ............................................................................................. 65 REFERENCES ...................................................................................................... 65 3 FLUORESCENCE NANOMETROLOGY IN SOL-GELS.................................................... 69 D.J.S. B IRCH , C.D. G EDDES , J. K AROLIN , R. L EISHMAN , AND O.J. R OLINSKI 3.1 INTRODUCTION............................................................................................ 70 3.2 SOL-GEL CHEMISTRY................................................................................... 72 3.3 ANISOTROPY THEORY................................................................................. 75 3.4 FRET DONOR-ACCEPTOR DISTRIBUTION THEORY........................................... 76 3.5 ACIDIC HYDROGELS ................................................................................... 78 3.6 ALKALINE HYDROGELS ................................................................................ 79 3.7 ALKOXIDE ALCOGELS.................................................................................. 80 3.8 WET PORE METROLOGY............................................................................... 81 3.9 CONCLUSIONS............................................................................................ 83 REFERENCES....................................................................................................... 84 4 INTEGRATED SUPRAMOLECULAR SYSTEMS: FROM SENSORS TO SWITCHES.............. 87 J.-P. M ALVAL , I. G OSSE , J.-P. M ORAND , R. L APOUYADE 4.1 INTRODUCTION............................................................................................ 88 4.2 OPTICAL DETECTION OF ION-GROUND STATE PROBE INTERACTION ..................... 90 4.3 CATION SENSING FROM FLUORESCENT PHOTOINDUCED INTRAMOLECULAR CHARGE TRANSFER (PICT) SENSORS............................................................ 91 4.4 OPTICAL AND ELECTROCHEMICAL RELEASE OF CATIONS.................................... 96 4.5 CONCLUSIONS............................................................................................ 97 REFERENCES....................................................................................................... 98 CONTENTS XI 5 RATIOMETRIC PROBES: DESIGN AND APPLICATIONS.......................................... 101 A. P. D EMCHENKO , A. S. K LYMCHENKO , V. G. P IVOVARENKO , AND S. E RCELEN 5.1 INTRODUCTION.......................................................................................... 102 5.2 3-HYDROXYFLAVONES AND OTHER 3-HYDROXYCHROMONE DERIVATIVES. NEW COMPOUNDS AND THEIR PROPERTIES................................................. 103 5.3 A NEW LEVEL OF SENSITIVITY TO SOLVENT POLARITY................................... 104 5.4 AMPLIFICATION BY ESIPT OF ELECTROCHROMIC EFFECTS............................. 106 5.5 MOLECULAR ORDER AND DYNAMICS IN PHOSPHOLIPID MEMBRANES.............. 107 5.6 AMPLIFICATION BY ESIPT OF SITE-SELECTIVE RED EDGE EFFECT................. 108 5.7 CONCLUSIONS.......................................................................................... 109 REFERENCES..................................................................................................... 110 6 BINDING OF ETHIDIUM TO YEAST TRNA PHE : A NEW PERSPECTIVE ON AN OLD BROMIDE................................................................................................. 111 M. T RAMIER , O. H OLUB , J. C. C RONEY , T. I SHI , S. E. S EIFRIED , AND D. M. J AMESON 6.1 OVERVIEW.............................................................................................. 112 6.2 EXPERIMENTAL........................................................................................ 112 6.2.1 SAMPLE PREPARATION................................................................... 112 6.2.2 MULTIFREQUENCY PHASE AND MODULATION FLUOROMETRY ................ 112 6.2.3 THREE COMPONENT ANALYSIS....................................................... 113 6.3 NEW ANALYSIS....................................................................................... 116 6.4 THE MODEL............................................................................................ 118 6.5 EFFECT OF IONIC STRENGTH........................................................................ 118 6.6 CONCLUSIONS.......................................................................................... 119 REFERENCES..................................................................................................... 120 7 EXPERIMENTAL ASPECTS OF DNA COMPUTING BY BLOCKING: USE OF FLUORESCENCE TECHNIQUES FOR DETECTION..................................................... 123 K. A. S CHMIDT , C. V . H ENKEL , G. R OZENBERG , AND H. P. S PAINK 7.1 INTRODUCTION.......................................................................................... 124 7.2 EXPERIMENTAL........................................................................................ 124 7.3 RESULTS AND DISCUSSION......................................................................... 125 7.4 CONCLUSION............................................................................................ 127 REFERENCES..................................................................................................... 128 PART 2 FLUORESCENCE SPECTROSCOPY OF SINGLE MOLECULES AND MOLECULAR ASSEMBLIES 8 MULTIPARAMETRIC DETECTION OF FLUORESCENCE EMITTED FROM INDIVIDUAL MULTICHROMOPHORIC SYSTEMS ...................................................................... 131 M. C OTLET , J. H OFKENS , M. M AUS , AND F. C. DE S CHRYVER 8.1 INTRODUCTION.......................................................................................... 132 8.2 MATERIALS AND METHODS......................................................................... 133 CONTENTS XII 8.2.1 SAMPLE PREPARATION................................................................... 133 8.2.2 EXPERIMENTAL SET-UP.................................................................. 133 8.3 DATA PROCESSING AND ANALYSIS.............................................................. 136 8.3.1 PULSED EXCITATION ...................................................................... 136 8.3.2 CW EXCITATION........................................................................... 139 8.4 RESULTS AND DISCUSSION......................................................................... 141 8.4.1 PULSED EXCITATION ...................................................................... 142 8.4.2 CW EXCITATION........................................................................... 145 8.5 CONCLUSIONS.......................................................................................... 149 REFERENCES..................................................................................................... 150 9 FLUORESCENCE INTENSITY DISTRIBUTION ANALYSIS (FIDA) AND RELATED FLUORESCENCE FLUCTUATION TECHNIQUES: THEORY AND PRACTICE........................ 153 P. K ASK , C. E GGELING , K. P ALO , UE. M ETS , M. C OLE , AND K. G ALL 9.1 INTRODUCTION.......................................................................................... 154 9.2 INSTRUMENTATION FOR FLUORESCENCE FLUCTUATION SPECTROSCOPY ............... 156 9.3 ASSUMPTIONS AND CONVENTIONS............................................................. 158 9.4 GENERATING FUNCTIONS FOR FIDA AND RELATED METHODS......................... 160 9.5 FIDA.................................................................................................... 162 9.6 2D-FIDA.............................................................................................. 165 9.7 FIMDA................................................................................................. 168 9.8 FILDA ................................................................................................. 172 9.9 CONCLUSIONS.......................................................................................... 178 REFERENCES..................................................................................................... 178 10 SINGLE MOLECULE REACTIONS OF THE ENZYME LDH AND OF RESTRICTION ENDONUCLEASES IN THE FLUORESCENCE MICROSCOPE ....................................... 183 B. N ASANSHARGAL , B. S CHAEFER , AND K. O. G REULICH 10.1 INTRODUCTION.......................................................................................... 184 10.2 FEMTODROPLETS AND THE POISSON STATISTICS ............................................. 185 10.3 FROM CONCENTRATIONS TO INTERMOLECULAR DISTANCES............................... 186 10.4 WHY FLUORESCENCE MICROSCOPY?.......................................................... 187 10.5 SINGLE MOLECULE ENZYME REACTIONS WITH SMALL SUBSTRATES................. 187 10.6 RESTRICTION ENDONUCLEASE REACTIONS .................................................... 191 10.7 CONCLUSIONS.......................................................................................... 194 REFERENCES..................................................................................................... 195 11 MONITORING YY -SUBUNIT MOVEMENT IN RECONSTITUTED SINGLE EF O F 1 ATP SYNTHASE BY FLUORESCENCE RESONANCE ENERGY TRANSFER........................... 197 M. B OERSCH , M. D IEZ , B. Z IMMERMANN , R. R EUTER , AND P. G RAEBER 11.1 INTRODUCTION.......................................................................................... 198 11.2 VISUALIZING INTERSUBUNIT ROTATION ........................................................ 198 11.3 FRET-LABELED F O F 1 ATP SYNTHASE EF O -B64-TMR-F 1 - YY 106-CY5.......... 200 11.3.1 SYNTHEZISING ATP WITH RECONSTITUTED EF O -B64-TMR- F 1 - YY 106-CY5.............................................................................. 201 11.3.2 SET-UP FOR SINGLE ENZYME FRET ANALYSIS................................ 201 CONTENTS XIII 11.3.3 DISCRIMINATION OF THREE YY -SUBUNIT POSITIONS WITH AMPPNP... 203 11.3.4 UNIDIRECTIONAL MOTION OF THE YY -SUBUNIT WITH ATP.................... 205 11.4 CONCLUSIONS.......................................................................................... 206 REFERENCES..................................................................................................... 207 PART 3 APPLICATION OF FLUORESCENCE IN BIOLOGICAL MEMBRANE AND ENZYME STUDIES 12 APPLICATION OF THE WAVELENGTH-SELECTIVE FLUORESCENCE APPROACH TO MONITOR MEMBRANE ORGANIZATION AND DYNAMICS.................................... 211 A. C HATTOPADHYAY 12.1 INTRODUCTION.......................................................................................... 212 12.2 RED EDGE EXCITATION SHIFT (REES)....................................................... 212 12.3 THE WAVELENGTH-SELECTIVE FLUORESCENCE APPROACH.............................. 214 12.4 THE WAVELENGTH-SELECTIVE FLUORESCENCE APPROACH: A NOVEL TOOL TO MONITOR ORGANIZATION AND DYNAMICS OF THE MEMBRANE INTERFACIAL REGION ................................................................................................. 214 12.5 WAVELENGTH-SELECTIVE FLUORESCENCE AS A MEMBRANE DIPSTICK.............. 217 12.6 APPLICATION OF THE WAVELENGTH-SELECTIVE FLUORESCENCE APPROACH TO MEMBRANE PEPTIDES AND PROTEINS ......................................................... 218 12.7 WAVELENGTH-SELECTIVE FLUORESCENCE IN MICELLES................................... 220 12.8 CONCLUSIONS.......................................................................................... 221 REFERENCES..................................................................................................... 221 13 FLUORESCENCE APPROACHES FOR THE CHARACTERIZATION OF THE PERIPHERAL MEMBRANE BINDING OF PROTEINS APPLIED FOR THE BLOOD COAGULATION PROTEIN PROTHROMBIN................................................................................... 225 R. H UTTERER AND M. H OF 13.1 INTRODUCTION.......................................................................................... 226 13.2 PROTHROMBIN BINDING TO NEGATIVELY CHARGED MEMBRANE SURFACE CHARACTERIZED BY PROTEIN FLUORESCENCE................................................ 227 13.2.1 INTRINSIC PROTEIN FLUORESCENCE: PICOSECOND TRYPTOPHAN FLUORESCENCE OF MEMBRANE-BOUND PROTHROMBIN FRAGMENT 1 (F1) ........................................................................ 227 13.2.2 OVERVIEW ON INVESTIGATIONS APPLYING FLUORESCENTLY LABELED PROTHROMBIN .............................................................................. 231 13.3 PROTHROMBIN-INDUCED CHANGES IN THE ORGANIZATION OF PHOSPHOLIPID BILAYERS ................................................................................................ 233 13.3.1 SOLVENT RELAXATION (SR)............................................................ 233 13.3.1.1 SOLVENT RELAXATION PROBED BY THE HEADGROUP LABELS PRODAN AND PATMAN ......................................... 233 13.3.1.2 INFLUENCE OF PROTHROMBIN AND ITS FRAGMENT 1 ON THE PHOSPOLIPID HEADGROUP ORGANIZATION................... 235 13.3.2 FLUORESCENCE ANISOTROPY: INFLUENCE OF PROTHROMBIN AND ITS FRAGMENT 1 ON PC/PS MEMBRANE ORDER ................................... 237 CONTENTS XIV 13.3.3 PYRENE FLUORESCENCE: INFLUENCE OF FRAGMENT 1 ON MEMBRANE ORDER MONITORED BY THE EXCIMER/MONOMER RATIO.................... 237 13.4 CONCLUSIONS.......................................................................................... 238 REFERENCES..................................................................................................... 239 14 ASSESSMENT OF MEMBRANE FLUIDITY IN INDIVIDUAL YEAST CELLS BY LAURDAN GENERALISED POLARISATION AND MULTI-PHOTON SCANNING FLUORESCENCE MICROSCOPY............................................................................ 241 R. P. L EARMONTH AND E. G RATTON 14.1 INTRODUCTION.......................................................................................... 242 14.1.1 YEAST MEMBRANE FLUIDITY.......................................................... 242 14.1.2 MULTI-PHOTON SCANNING FLUORESCENCE MICROSCOPY ................... 243 14.1.3 DETERMINATION OF MEMBRANE FLUIDITY USING LAURDAN GENERALIZED POLARIZATION .......................................................... 244 14.2 MATERIALS AND METHODS......................................................................... 244 14.3 RESULTS AND DISCUSSION......................................................................... 246 14.4 CONCLUSIONS.......................................................................................... 250 REFERENCES..................................................................................................... 251 15 FORMATION OF HIGHER ORDER SIGNAL TRANSDUCTION COMPLEXES AS SEEN BY FLUORESCENCE SPECTROSCOPY........................................................... 253 L. D OWAL AND S. S CARLATA 15.1 INTRODUCTION.......................................................................................... 254 15.2 EXPERIMENTAL........................................................................................ 256 15.2.1 MEMBRANE BINDING ................................................................... 257 15.2.2 INTERACTIONS OF PLC YY AND G PROTEIN SUBUNITS ON MEMBRANE SURFACE.................................................................... 257 15.2.3 PLC YY 2 -G YYYY ASSOCIATIONS ON MEMBRANE SURFACES..................... 258 15.2.4 EFFECTS OF LIPID RAFTS ON PROTEIN ASSOCIATION........................... 259 REFERENCES..................................................................................................... 260 16 MECHANISMS OF THE MODULATION OF MEMBRANE INTERFACIAL ENZYME CATALYSIS BY NON-LAMELLAR FORMING LIPIDS: COMPARISON WITH THE BEHAVIOR OF A FLUORESCENT PROBE IN MEMBRANES ...................................... 263 R. M. E PAND , R. C ORNELL , S. M.A. D AVIES , AND R. K RAAYENHOF 16.1 INTRODUCTION.......................................................................................... 264 16.1.1 SPECIFIC VS. NON-SPECIFIC MODULATION OF PROTEIN ACTIVITY........ 264 16.1.2 NON-LAMELLAR FORMING LIPIDS ................................................... 265 16.2 SPECIFIC PROTEIN EXAMPLES ................................................................... 266 16.2.1 PROTEIN KINASE C....................................................................... 266 16.2.2 PHOSPHOCHOLINE CYTIDYLYLTRANSFERASE........................................ 267 16.3 MOLECULAR MECHANISMS OF ENZYME ACTIVATION BY NON-LAMELLAR FORMING LIPIDS..................................................................................... 268 16.3.1 CURVATURE STRAIN........................................................................ 268 16.3.2 LATERAL PRESSURE PROFILE............................................................. 269 16.3.3 OTHER MECHANISMS.................................................................... 269 CONTENTS XV 16.4 CRITERIA FOR THE ROLE OF MEMBRANE CURVATURE STRAIN............................ 269 16.4.1 CUBIC PHASES............................................................................. 269 16.4.2 HOMOLOGOUS LIPIDS................................................................... 271 16.5 RELATIONSHIP OF THE PROPERTIES OF A FLUORESCENT INTERFACIAL MEMBRANE PROBE .................................................................................................... 271 16.6 COMPARISON OF THE MECHANISMS OF ACTIVATION OF PKC AND CT BY NON-LAMELLAR FORMING LIPIDS............................................................... 272 16.7 CONCLUSIONS.......................................................................................... 273 REFERENCES..................................................................................................... 274 17 EMISSION SPECTROSCOPY OF COMPLEX FORMATION BETWEEN ESCHERICHIA COLI PURINE NUCLEOSIDE PHOSPHORYLASE (PNP) AND IDENTIFIED TAUTOMERIC SPECIES OF FORMYCIN INHIBITORS RESOLVES AMBIGUITIES FOUND IN CRYSTALLOGRAPHIC STUDIES............................................................................. 277 B. K IERDASZUK 17.1 INTRODUCTION.......................................................................................... 278 17.2 FORMYCIN A AND ITS N -METHYL ANALOGS, SPECIFIC INHIBITORS OF E. COLI PNP........................................................................................... 280 17.3 AMBIGUITIES FOUND IN THE CRYSTALLOGRAPHIC STRUCTURE OF ENZYME- LIGAND COMPLEX .................................................................................. 281 17.4 SOLUTION STRUCTURE OF INHIBITORS BOUND BY THE ENZYME........................ 283 17.4.1 TAUTOMERIC EQUILIBRIA, AND ABSORPTION AND EMISSION SPECTRA OF THE TAUTOMERIC SPECIES IN SOLUTION ...................................... 283 17.4.2 SHIFTS BETWEEN ABSORPTION AND EMISSION SPECTRA OF THE ENZYME AND LIGANDS................................................................. 286 17.4.3 EFFECT OF BINDING OF THE IDENTIFIED TAUTOMERIC SPECIES ON THEIR FLUORESCENCE AND PHOSPHORESCENCE.................................. 287 17.5 CONCLUSIONS.......................................................................................... 291 REFERENCES..................................................................................................... 292 PART 4 MICROSCOPIC IMAGING TECHNIQUES AND THEIR APPLICATION FOR THE STUDY OF LIVING CELLS 18 FLUORESCENCE LIFETIME IMAGING IMPLEMENTED WITH RESONANT GALVANOMETER SCANNERS ............................................................................. 297 J. J. B IRMINGHAM 18.1 INTRODUCTION.......................................................................................... 298 18.2 THEORY.................................................................................................. 299 18.3 SIMULATIONS........................................................................................... 305 18.4 EXPERIMENTAL SET-UP............................................................................. 308 18.5 RESULTS.................................................................................................. 311 18.6 CONCLUSIONS.......................................................................................... 314 REFERENCES..................................................................................................... 315 CONTENTS XVI 19 SPECTRAL IMAGING OF SINGLE CDSE/ZNS QUANTUM DOTS EMPLOYING SPECTRALLY- AND TIME-RESOLVED CONFOCAL MICROSCOPY.............................. 317 W.G.J.H.M. VAN S ARK , P.L.T.M. F REDERIX , M.A.H. A SSELBERGS , D.J. VAN DEN H EUVEL , A. M EIJERINK , AND H. C. G ERRITSEN 19.1 INTRODUCTION.......................................................................................... 318 19.2 EXPERIMENTAL........................................................................................ 319 19.2.1 SPECTROGRAPH-CLSM SET-UP...................................................... 319 19.2.1.1 DESCRIPTION................................................................. 319 19.2.1.2 PERFORMANCE ............................................................... 322 19.2.2 QD SYNTHESIS AND CHARACTERIZATION .......................................... 324 19.3 RESULTS AND DISCUSSION......................................................................... 325 19.4 CONCLUSION ........................................................................................... 332 REFERENCES..................................................................................................... 332 20 IMAGING OF OXIDATIVE STRESS IN PLANT CELLS BY QUANTITATIVE FLUORESCENCE MICROSCOPY AND SPECTROSCOPY............................................. 337 J. W. B ORST , M. A. U SKOVA , N. V. V ISSER , AND A. J. W. G. V ISSER 20.1 INTRODUCTION.......................................................................................... 338 20.2 EXPERIMENTAL PROCEDURES...................................................................... 340 20.2.1 TOBACCO BY-2 CELLS.................................................................. 340 20.2.2 PREPARATION OF CELL SUSPENSION................................................. 340 20.2.3 PROTOPLAST ISOLATION................................................................... 341 20.2.4 LABELING CELLS WITH BP-C11..................................................... 341 20.2.5 CONFOCAL MICROSCOPY................................................................ 341 20.2.6 STEADY-STATE FLUORESCENCE......................................................... 342 20.3 RESULTS AND DISCUSSION......................................................................... 342 20.3.1 MEASUREMENTS AND IMAGING OF OXIDATIVE STRESS IN TOBACCO BY-2 CELLS.................................................................. 342 20.3.2 EFFECT OF INHIBITORS AND ROS SCAVENGERS ON THE HYDROGEN PEROXIDE PRODUCTION IN BY-2 CELLS........................................... 344 20.4 CONCLUSIONS.......................................................................................... 346 REFERENCES..................................................................................................... 347 21 THE BIOMEDICAL USE OF RESCALING PROCEDURES IN OPTICAL BIOPSY AND OPTICAL MOLECULAR IMAGING ........................................................................ 349 O. M INET , J. B EUTHAN , K. L ICHA , AND C. M AHNKE 21.1 INTRODUCTION.......................................................................................... 350 21.2 METHOD OF RESCALING............................................................................ 350 21.3 BIOMEDICAL EXAMPLES........................................................................... 352 21.3.1 OPTICAL BIOPSY IN THE UV RANGE USING ENDOGENOUS CHROMOPHORES........................................................................... 352 21.3.1.1 EXPERIMENT ................................................................ 353 21.3.1.2 RESULTS........................................................................ 354 21.3.2 OPTICAL MOL E CULAR IMAGING IN THE NIR RANGE USING EXOGENOUS CONTRAST AGENTS...................................................... 354 21.3.2.1 EXPERIMENT................................................................. 356 CONTENTS XVII 21.3.2.2 RESULTS........................................................................ 357 21.4 DISCUSSION AND CONCLUSIONS................................................................. 358 REFERENCES..................................................................................................... 359 22 LOOKING INTO A LIVING CELL ......................................................................... 361 M. VAN B ORREN , N. R. B RADY , J. R AVELSLOOT , AND H. V. W ESTERHOFF 22.1 INTRODUCTION.......................................................................................... 362 22.2 HOW TO CHOOSE YOUR FLUORESCENT INDICATOR.......................................... 362 22.2.1 PROCESS OF INTEREST..................................................................... 362 22.2.2 RATIOMETRIC DYES....................................................................... 363 22.2.3 BUFFERING POWER........................................................................ 364 22.2.4 PHOTO-TOXICITY............................................................................ 364 22.3 CONSIDERATIONS CONCERNING THE EXPERIMENTAL APPROACH...................... 364 22.3.1 SELECTIVE LOADING...................................................................... 364 22.3.2 ADDITIONAL LOADING TECHNIQUES................................................ 366 22.3.3 THE IMAGING SYSTEM................................................................. 366 22.4 EXAMPLES OF FLUORESCENCE MICROSCOPY IN LIVING CELLS....................... 366 22.4.1 DETECTION OF THE CA 2+ ION .......................................................... 366 22.4.2 DETERMINATION OF INTRACELLULAR PH ............................................ 367 22.4.3 MITOCHONDRIAL ENERGETICS.......................................................... 368 22.4.4 REACTIVE OXYGEN SPECIES (ROS)............................................... 370 22.4.5 AUTOFLUORESCENCE: MARKER OF REDOX STATE................................ 371 22.5 CONCLUSIONS.......................................................................................... 371 REFERENCES..................................................................................................... 371 23 EXPRESSION OF MULTICOLOR FLUORESCENT FUSION PROTEINS IN ZEBRAFISH CELL CULTURES: A VERSATILE TOOL IN CELL BIOLOGY........................................ 373 C. K. D. B REEK , F. VAN I REN , S. E. W IJTING , N. S TUURMAN , AND H. P. S PAINK 23.1 INTRODUCTION.......................................................................................... 374 23.2 ZEBRAFISH CELL LINES............................................................................. 374 23.3 MICROSCOPICAL ANALYSIS........................................................................ 376 23.4 ANALYSIS OF TRANSFECTED CELL LINES ...................................................... 377 23.5 CONCLUSIONS.......................................................................................... 379 REFERENCES..................................................................................................... 379 SUBJECT INDEX..................................................................................................... 381
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genre	(DE-588)1071861417 Konferenzschrift 2001 Amsterdam gnd-content
genre_facet	Konferenzschrift 2001 Amsterdam
id	DE-604.BV014393337
illustrated	Illustrated
indexdate	2024-07-09T19:02:21Z
institution	BVB
isbn	3540427686
language	English
oai_aleph_id	oai:aleph.bib-bvb.de:BVB01-009852732
oclc_num	443705303
open_access_boolean
owner	DE-19 DE-BY-UBM DE-M347 DE-526 DE-11
owner_facet	DE-19 DE-BY-UBM DE-M347 DE-526 DE-11
physical	XXV, 390 S. Ill., graph. Darst.
publishDate	2002
publishDateSearch	2002
publishDateSort	2002
publisher	Springer
record_format	marc
series	Springer series on fluorescence
series2	Springer series on fluorescence
spelling	Fluorescence spectroscopy, imaging and probes new tools in chemical, physical and life sciences Ruud Kraayenhof ... (eds.) Berlin [u.a.] Springer 2002 XXV, 390 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Springer series on fluorescence 2 Fluorescence spectroscopy Congresses Fluoreszenzspektroskopie (DE-588)4017701-4 gnd rswk-swf (DE-588)1071861417 Konferenzschrift 2001 Amsterdam gnd-content Fluoreszenzspektroskopie (DE-588)4017701-4 s DE-604 Kraayenhof, Ruud Sonstige oth Springer series on fluorescence 2 (DE-604)BV013838103 2 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009852732&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Fluorescence spectroscopy, imaging and probes new tools in chemical, physical and life sciences Springer series on fluorescence Fluorescence spectroscopy Congresses Fluoreszenzspektroskopie (DE-588)4017701-4 gnd
subject_GND	(DE-588)4017701-4 (DE-588)1071861417
title	Fluorescence spectroscopy, imaging and probes new tools in chemical, physical and life sciences
title_auth	Fluorescence spectroscopy, imaging and probes new tools in chemical, physical and life sciences
title_exact_search	Fluorescence spectroscopy, imaging and probes new tools in chemical, physical and life sciences
title_full	Fluorescence spectroscopy, imaging and probes new tools in chemical, physical and life sciences Ruud Kraayenhof ... (eds.)
title_fullStr	Fluorescence spectroscopy, imaging and probes new tools in chemical, physical and life sciences Ruud Kraayenhof ... (eds.)
title_full_unstemmed	Fluorescence spectroscopy, imaging and probes new tools in chemical, physical and life sciences Ruud Kraayenhof ... (eds.)
title_short	Fluorescence spectroscopy, imaging and probes
title_sort	fluorescence spectroscopy imaging and probes new tools in chemical physical and life sciences
title_sub	new tools in chemical, physical and life sciences
topic	Fluorescence spectroscopy Congresses Fluoreszenzspektroskopie (DE-588)4017701-4 gnd
topic_facet	Fluorescence spectroscopy Congresses Fluoreszenzspektroskopie Konferenzschrift 2001 Amsterdam
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009852732&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
volume_link	(DE-604)BV013838103
work_keys_str_mv	AT kraayenhofruud fluorescencespectroscopyimagingandprobesnewtoolsinchemicalphysicalandlifesciences

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