Mitochondrial DNA: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, N.J.
Humana Press
2002
|
| Schriftenreihe: | Methods in molecular biology
197 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XIV, 414 S. Ill., graph. Darst. |
| ISBN: | 0896039722 |
Internformat
MARC
| LEADER | 00000nam a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV014387807 | ||
| 003 | DE-604 | ||
| 005 | 20070831 | ||
| 007 | t | ||
| 008 | 020603s2002 xxuad|| |||| 00||| eng d | ||
| 010 | |a 2002020564 | ||
| 020 | |a 0896039722 |9 0-89603-972-2 | ||
| 035 | |a (OCoLC)48958171 | ||
| 035 | |a (DE-599)BVBBV014387807 | ||
| 040 | |a DE-604 |b ger |e aacr | ||
| 041 | 0 | |a eng | |
| 044 | |a xxu |c US | ||
| 049 | |a DE-355 |a DE-12 |a DE-11 | ||
| 050 | 0 | |a QH603.M5 | |
| 082 | 0 | |a 572.8/6 |2 21 | |
| 084 | |a WC 4150 |0 (DE-625)148092: |2 rvk | ||
| 084 | |a WC 4460 |0 (DE-625)148102: |2 rvk | ||
| 084 | |a WD 5360 |0 (DE-625)148202: |2 rvk | ||
| 245 | 1 | 0 | |a Mitochondrial DNA |b methods and protocols |c edited by William C. Copeland |
| 264 | 1 | |a Totowa, N.J. |b Humana Press |c 2002 | |
| 300 | |a XIV, 414 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 197 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a ADN mitochondrial - Manuels de laboratoire | |
| 650 | 4 | |a Acido desoxirribonucleico - Manuales de laboratorio | |
| 650 | 7 | |a DNA |2 gtt | |
| 650 | 7 | |a Mitochondriën |2 gtt | |
| 650 | 4 | |a Mitocondria - Manuales de laboratorio | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a DNA, Mitochondrial |v Laboratory Manuals | |
| 650 | 4 | |a Mitochondrial DNA |v Laboratory manuals | |
| 650 | 0 | 7 | |a Mitochondriale DNS |0 (DE-588)4202301-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Mitochondriopathie |0 (DE-588)4280150-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Labortechnik |0 (DE-588)4123602-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Genanalyse |0 (DE-588)4200230-8 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Mitochondriale DNS |0 (DE-588)4202301-4 |D s |
| 689 | 0 | 1 | |a Labortechnik |0 (DE-588)4123602-6 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Mitochondriopathie |0 (DE-588)4280150-3 |D s |
| 689 | 1 | 1 | |a Mitochondriale DNS |0 (DE-588)4202301-4 |D s |
| 689 | 1 | 2 | |a Genanalyse |0 (DE-588)4200230-8 |D s |
| 689 | 1 | |5 DE-604 | |
| 700 | 1 | |a Copeland, William C. |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in molecular biology |v 197 |w (DE-604)BV035362695 |9 197 | |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009848631&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-009848631 | ||
Datensatz im Suchindex
| _version_ | 1804129256789245952 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
Part I. Methods for the Analysis of mtDNA
1 Animal Models for Mitochondrial Disease
Douglas C. Wallace 3
2 Identification of Mutations in mtDNA from Patients
Suffering Mitochondrial Diseases
Eric A. Schon, AH Naini, and Sara Shanske 55
3 Screening for Aging Dependent Point Mutations in mtDNA
Yuichi Michikawa and Giuseppe Attardi 75
4 Scanning Low Frequency Point Mutants in the Mitochondrial Genome
Using Constant Denaturant Capillary Electrophoresis
Weiming Zheng, Luisa A. Marcelino, and William G. Thilly 93
5 Identification of mtDNA Mutations in Human Cancer
Shuji Nomoto, Montserrant Sanchez Cespedes,
and David Sidransky 107
6 In Situ PCR of the Common Human mtDNA Deletion:
Is It Related to Apoptosis?
Steven J. Zullo 119
7 Reproducible Quantitative PCR of Mitochondrial
and Nuclear DNA Copy Number Using the LightCycler™
Alice Wong and Gino Cortopassi 129
8 Yeast Nuclear Genes for mtDNA Maintenance
Frangoise Foury 139
9 Measuring mtDNA Mutation Rates in Saccharomyces cerevisiae
Using the mtArg8 Assay
Micheline K. Strand and William C. Copeland 151
10 Measuring Oxidative mtDNA Damage and Repair
Using Quantitative PCR
Janine H. Santos, Bhaskar S. Mandavilli,
and Bennett Van Houten 159
viii Contents
11 Migration of mtDNA into the Nucleus
Mary K. Thorsness, Karen H. White, and Peter E. Thorsness 177
12 2D Gel Electrophoresis of mtDNA
Heather E. Lorimer 187
Part II. Methods for the Analysis of Proteins Involved
in mtDNA Transactions
13 Purification of Mitochondria for Enzymes Involved
in Nucleic Acid Transactions
Daniel F. Bogenhagen 199
14 Purification of Mitochondrial Uracil DNA Glycosylase
Using Ugi Sepharose Affinity Chromatography
Samuel E. Bennett, Mary Jane Shroyer, Jung Suk Sung,
and Dale W. Mosbaugh 211
15 Characterization of Specialized mtDNA Glycosylases
Simon G. Nyaga and Vilhelm A. Bohr 227
16 Purification, Separation, and Identification of the Human mtDNA
Polymerase With and Without Its Accessory Subunit
Matthew J. Longley and William C. Copeland 245
17 Assay of mtDNA Polymerase y from Human Tissues
Robert K. Naviaux 259
18 Purification Strategies for Drosophila mtDNA Replication Proteins
in Native and Recombinant Form: DNA Polymerase y
Carol L. Farr and Laurie S. Kaguni 273
19 Purification Strategies for Drosophila mtDNA Replication Proteins
in Native and Recombinant Form: Mitochondrial Single Stranded
DNA Binding Protein
Carol L. Farr and Laurie S. Kaguni 285
20 Expression, Purification, and In Vitro Assays of Mitochondrial
Single Stranded DNA Binding Protein
Kang Li and R. Sanders Williams 295
21 Recombinant Yeast mtDNA Helicases: Purification
and Functional Assays
Silja Kuusk, Tiina Sedman, and Juhan Sedman 303
22 Isolation of DNA Topoisomerases I and II Activities
from Mitochondria of Mammalian Heart
Robert L. Low 377
23 Endonuclease G Isolation and Assays
Robert L. Low and Mariana Gerschenson 331
Contents ix
24 Targeting DNA Repair Proteins to Mitochondria
Allison W. Dobson, Mark R. Kelley, Glenn L. Wilson,
and Susan P. LeDoux 351
25 In Organello Footprinting of mtDNA
Steven C. Ghivizzani, Cort S. Madsen,
Chandramohan V. Ammini, and William W. Hauswirth 363
26 Crosslinking of Proteins to mtDNA
Brett A. Kaufman, Scott M. Newman, Philip S. Perlman,
and Ronald A. Butow 377
27 Breeding and Genotyping of Tfam Conditional Knockout Mice
Mats Ekstrand and Nils Goran Larsson 391
Index 401
|
| any_adam_object | 1 |
| building | Verbundindex |
| bvnumber | BV014387807 |
| callnumber-first | Q - Science |
| callnumber-label | QH603 |
| callnumber-raw | QH603.M5 |
| callnumber-search | QH603.M5 |
| callnumber-sort | QH 3603 M5 |
| callnumber-subject | QH - Natural History and Biology |
| classification_rvk | WC 4150 WC 4460 WD 5360 |
| ctrlnum | (OCoLC)48958171 (DE-599)BVBBV014387807 |
| dewey-full | 572.8/6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.8/6 |
| dewey-search | 572.8/6 |
| dewey-sort | 3572.8 16 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02514nam a2200637zcb4500</leader><controlfield tag="001">BV014387807</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20070831 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">020603s2002 xxuad|| |||| 00||| eng d</controlfield><datafield tag="010" ind1=" " ind2=" "><subfield code="a">2002020564</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">0896039722</subfield><subfield code="9">0-89603-972-2</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)48958171</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV014387807</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">aacr</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">xxu</subfield><subfield code="c">US</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-11</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH603.M5</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572.8/6</subfield><subfield code="2">21</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4150</subfield><subfield code="0">(DE-625)148092:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4460</subfield><subfield code="0">(DE-625)148102:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WD 5360</subfield><subfield code="0">(DE-625)148202:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Mitochondrial DNA</subfield><subfield code="b">methods and protocols</subfield><subfield code="c">edited by William C. Copeland</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, N.J.</subfield><subfield code="b">Humana Press</subfield><subfield code="c">2002</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XIV, 414 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in molecular biology</subfield><subfield code="v">197</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Includes bibliographical references and index</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">ADN mitochondrial - Manuels de laboratoire</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Acido desoxirribonucleico - Manuales de laboratorio</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">DNA</subfield><subfield code="2">gtt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Mitochondriën</subfield><subfield code="2">gtt</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Mitocondria - Manuales de laboratorio</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Wetenschappelijke technieken</subfield><subfield code="2">gtt</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Wissenschaftliches Arbeiten</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">DNA, Mitochondrial</subfield><subfield code="v">Laboratory Manuals</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Mitochondrial DNA</subfield><subfield code="v">Laboratory manuals</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Mitochondriale DNS</subfield><subfield code="0">(DE-588)4202301-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Mitochondriopathie</subfield><subfield code="0">(DE-588)4280150-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Labortechnik</subfield><subfield code="0">(DE-588)4123602-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Genanalyse</subfield><subfield code="0">(DE-588)4200230-8</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Mitochondriale DNS</subfield><subfield code="0">(DE-588)4202301-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Labortechnik</subfield><subfield code="0">(DE-588)4123602-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="1" ind2="0"><subfield code="a">Mitochondriopathie</subfield><subfield code="0">(DE-588)4280150-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="1"><subfield code="a">Mitochondriale DNS</subfield><subfield code="0">(DE-588)4202301-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="2"><subfield code="a">Genanalyse</subfield><subfield code="0">(DE-588)4200230-8</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Copeland, William C.</subfield><subfield code="e">Sonstige</subfield><subfield code="4">oth</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in molecular biology</subfield><subfield code="v">197</subfield><subfield code="w">(DE-604)BV035362695</subfield><subfield code="9">197</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">HBZ Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009848631&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-009848631</subfield></datafield></record></collection> |
| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV014387807 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T19:02:15Z |
| institution | BVB |
| isbn | 0896039722 |
| language | English |
| lccn | 2002020564 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009848631 |
| oclc_num | 48958171 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-12 DE-11 |
| owner_facet | DE-355 DE-BY-UBR DE-12 DE-11 |
| physical | XIV, 414 S. Ill., graph. Darst. |
| publishDate | 2002 |
| publishDateSearch | 2002 |
| publishDateSort | 2002 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Mitochondrial DNA methods and protocols edited by William C. Copeland Totowa, N.J. Humana Press 2002 XIV, 414 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 197 Includes bibliographical references and index ADN mitochondrial - Manuels de laboratoire Acido desoxirribonucleico - Manuales de laboratorio DNA gtt Mitochondriën gtt Mitocondria - Manuales de laboratorio Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten DNA, Mitochondrial Laboratory Manuals Mitochondrial DNA Laboratory manuals Mitochondriale DNS (DE-588)4202301-4 gnd rswk-swf Mitochondriopathie (DE-588)4280150-3 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Genanalyse (DE-588)4200230-8 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Mitochondriale DNS (DE-588)4202301-4 s Labortechnik (DE-588)4123602-6 s DE-604 Mitochondriopathie (DE-588)4280150-3 s Genanalyse (DE-588)4200230-8 s Copeland, William C. Sonstige oth Methods in molecular biology 197 (DE-604)BV035362695 197 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009848631&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mitochondrial DNA methods and protocols Methods in molecular biology ADN mitochondrial - Manuels de laboratoire Acido desoxirribonucleico - Manuales de laboratorio DNA gtt Mitochondriën gtt Mitocondria - Manuales de laboratorio Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten DNA, Mitochondrial Laboratory Manuals Mitochondrial DNA Laboratory manuals Mitochondriale DNS (DE-588)4202301-4 gnd Mitochondriopathie (DE-588)4280150-3 gnd Labortechnik (DE-588)4123602-6 gnd Genanalyse (DE-588)4200230-8 gnd |
| subject_GND | (DE-588)4202301-4 (DE-588)4280150-3 (DE-588)4123602-6 (DE-588)4200230-8 (DE-588)4143413-4 |
| title | Mitochondrial DNA methods and protocols |
| title_auth | Mitochondrial DNA methods and protocols |
| title_exact_search | Mitochondrial DNA methods and protocols |
| title_full | Mitochondrial DNA methods and protocols edited by William C. Copeland |
| title_fullStr | Mitochondrial DNA methods and protocols edited by William C. Copeland |
| title_full_unstemmed | Mitochondrial DNA methods and protocols edited by William C. Copeland |
| title_short | Mitochondrial DNA |
| title_sort | mitochondrial dna methods and protocols |
| title_sub | methods and protocols |
| topic | ADN mitochondrial - Manuels de laboratoire Acido desoxirribonucleico - Manuales de laboratorio DNA gtt Mitochondriën gtt Mitocondria - Manuales de laboratorio Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten DNA, Mitochondrial Laboratory Manuals Mitochondrial DNA Laboratory manuals Mitochondriale DNS (DE-588)4202301-4 gnd Mitochondriopathie (DE-588)4280150-3 gnd Labortechnik (DE-588)4123602-6 gnd Genanalyse (DE-588)4200230-8 gnd |
| topic_facet | ADN mitochondrial - Manuels de laboratoire Acido desoxirribonucleico - Manuales de laboratorio DNA Mitochondriën Mitocondria - Manuales de laboratorio Wetenschappelijke technieken Wissenschaftliches Arbeiten DNA, Mitochondrial Laboratory Manuals Mitochondrial DNA Laboratory manuals Mitochondriale DNS Mitochondriopathie Labortechnik Genanalyse Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009848631&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT copelandwilliamc mitochondrialdnamethodsandprotocols |