Fundamentals of light microscopy and electronic imaging:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
New York
Wiley-Liss
2001
|
| Subjects: | |
| Online Access: | Publisher description Table of Contents Inhaltsverzeichnis |
| Physical Description: | XII, 368 S. zahlr. Ill. u. graph. Darst. |
| ISBN: | 047125391X |
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Record in the Search Index
| _version_ | 1804129244419194880 |
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| adam_text | FUNDAMENTALS OF LIGHT MICROSCOPY AND ELECTRONIC IMAGING DOUGLAS B.
MURPHY DEPARTMENT OF CELL BIOLOGY = IFIV.-NF. JOHNS HOPKINS UNIVERSITY
SCHOOL OF MEDICINE .......... UR.IVISRSITATS- UNCLLAND;;S- L IBLIOTHSK
DARMSTADT BIBLIOTHEK BIOLOGIC WILEY-LISS A JOHN WILEY & SONS, INC.,
PUBLICATION CONTENTS PREFACE XI 1. FUNDAMENTALS OF LIGHT MICROSCOPY 1
OVERVIEW 1 OPTICAL COMPONENTS OF THE LIGHT MICROSCOPE 1 NOTE: INVERTED
MICROSCOPE DESIGNS 3 J APERTURE AND IMAGE PLANES IN A FOCUSED, ADJUSTED
MICROSCOPE 4 NOTE: USING AN EYEPIECE TELESCOPE TO VIEW THE OBJECTIVE
BACK APERTURE 5 KOEHLER ILLUMINATION 6 ^ ADJUSTING THE MICROSCOPE FOR
KOEHLER ILLUMINATION 7 NOTE: SUMMARY OF STEPS FOR KOEHLER ILLUMINATION 7
NOTE: FOCUSING OIL IMMERSION OBJECTIVES 11 PRECAUTIONS FOR HANDLING
OPTICAL EQUIPMENT 11 EXERCISE: CALIBRATION OF MAGNIFICATION 12 2.
LIGHT AND COLOR 15 OVERVIEW 15 LIGHT AS A PROBE OF MATTER 15 LIGHT AS
PARTICLES AND WAVES 18 THE QUALITY OF LIGHT 20 PROPERTIES OF LIGHT
PERCEIVED BY THE EYE 21 PHYSICAL BASIS FOR VISUAL PERCEPTION AND COLOR
22 POSITIVE AND NEGATIVE COLORS 24 ; EXERCISE: COMPLEMENTARY COLORS 26
3. ILLUMINATORS, FILTERS, AND ISOLATION OF SPECIFIC WAVELENGTHS 29
OVERVIEW 29 ILLUMINATORS AND THEIR SPECTRA 29 VI CONTENTS DEMONSTRATION:
SPECTRA OF COMMON LIGHT SOURCES 33 ILLUMINATOR ALIGNMENT AND BULB
REPLACEMENT 34 DEMONSTRATION: ALIGNING A 100 WMERCURY ARC LAMP IN AN
EPI-ILLUMINATOR 35 FIRST ON*LAST OFF : ESSENTIAL RULE FOR ARC LAMP
POWER SUPPLIES 36 FILTERS FOR ADJUSTING THE INTENSITY AND WAVELENGTH OF
ILLUMINATION 37 EFFECTS OF LIGHT ON LIVING CELLS 41 4. LENSES AND
GEOMETRICAL OPTICS 43 OVERVIEW 43 IMAGE FORMATION BY A SIMPLE LENS 43
NOTE: REAL AND VIRTUAL IMAGES 45 RULES OF RAY TRACING FOR A SIMPLE LENS
46 OBJECT-IMAGE MATH 46 THE PRINCIPAL ABERRATIONS OF LENSES 50 DESIGNS
AND SPECIFICATIONS OF OBJECTIVE LENSES 53 CONDENSERS 56 OCULARS 56
MICROSCOPE SLIDES AND COVERSLIPS 57 THE CARE AND CLEANING OF OPTICS 58
EXERCISE: CONSTRUCTING AND TESTING AN OPTICAL BENCH MICROSCOPE 59 5.
DIFFRACTION AND INTERFERENCE IN IMAGE FORMATION 61 OVERVIEW 61 DEFINING
DIFFRACTION AND INTERFERENCE 61 THE DIFFRACTION IMAGE OF A POINT SOURCE
OF LIGHT 64 DEMONSTRATION: VIEWING THE AIRY DISK WITH A PINHOLE
APERTURE 66 CONSTANCY OF OPTICAL PATH LENGTH BETWEEN THE OBJECT AND THE
IMAGE 68 EFFECT OF APERTURE ANGLE ON DIFFRACTION SPOT SIZE 69
DIFFRACTION BY. A GRATING AND CALCULATION OF ITS LINE SPACING, D 71
DEMONSTRATION: THE DIFFRACTION GRATING 75 ABBE S THEORY FOR IMAGE
FORMATION IN THE MICROSCOPE 77 DIFFRACTION PATTERN FORMATION IN THE BACK
APERTURE OF THE OBJECTIVE LENS 80 DEMONSTRATION: OBSERVING THE
DIFFRACTION IMAGE IN THE BACK FOCAL , PLANE OF A LENS 81 PRESERVATION OF
COHERENCE: AN ESSENTIAL REQUIREMENT FOR IMAGE FORMATION 82 EXERCISE:
DIFFRACTION BY MICROSCOPE SPECIMENS 84 6. DIFFRACTION AND SPATIAL
RESOLUTION 85 OVERVIEW 85 NUMERICAL APERTURE 85 SPATIAL RESOLUTION 87
DEPTH OF FIELD AND DEPTH OF FOCUS 90 OPTIMIZING THE MICROSCOPE IMAGE: A
COMPROMISE BETWEEN SPATIAL RESOLUTION AND CONTRAST 91 EXERCISE:
RESOLUTION OF STRIAE IN DIATOMS 93 CONTENTS VII 7. PHASE CONTRAST
MICROSCOPY AND DARK-FIELD MICROSCOPY 97 OVERVIEW 97 PHASE CONTRAST
MICROSCOPY 97 . ** THE BEHAVIOR OF WAVES FROM PHASE OBJECTS IN
BRIGHT-FIELD MICROSCOPY 99 THE ROLE OF DIFFERENCES IN OPTICAL PATH
LENGTHS 103 THE OPTICAL DESIGN OF THE PHASE CONTRAST MICROSCOPE 103
ALIGNMENT 106 INTERPRETATING THE PHASE CONTRAST IMAGE 106 EXERCISE:
DETERMINATION OF THE INTRACELLULAR CONCENTRATION OF HEMOGLOBIN IN
ERYTHROCYTES BY PHASE IMMERSION REFRACTOMETRY 110 DARK-FIELD MICROSCOPY
112 THEORY AND OPTICS 112 IMAGE INTERPRETATION 115 EXERCISE: DARK-FIELD
MICROSCOPY 116 8. PROPERTIES OF POLARIZED LIGHT 117 OVERVIEW 117 THE
GENERATION OF POLARIZED LIGHT 117 O DEMONSTRATION: PRODUCING POLARIZED
LIGHT WITH A POLAROID FILTER 119 POLARIZATION BY REFLECTION AND
SCATTERING^ 121 VECTORIAL ANALYSIS OF POLARIZED LIGHT USING A DICHROIC
FILTER 121 DOUBLE REFRACTION IN CRYSTALS 124 DEMONSTRATION: DOUBLE
REFRACTION BY A CALCITE CRYSTAL 126 KINDS OF BIREFRINGENCE 127
PROPAGATION OF O AND E WAVEFRONTS IN A BIREFRINGENT CRYSTAL 128
BIREFRINGENCE IN BIOLOGICAL SPECIMENS 130 GENERATION OF ELLIPTICALLY
POLARIZED LIGHT BY BIREFRINGENT SPECIMENS 131 9. POLARIZATION MICROSCOPY
135 OVERVIEW 135 OPTICS OF THE POLARIZING MICROSCOPE 136 ADJUSTING THE
POLARIZING MICROSCOPE 138 APPEARANCE OF BIREFINGENT OBJECTS IN POLARIZED
LIGHT 139 PRINCIPLES OF ACTION OF RETARDATION PLATES AND THREE POPULAR
COMPENSATORS 139 DEMONSTRATION: MAKING A A PLATE FROM A PIECE OF
CELLOPHANE 143 EXERCISE: DETERMINATION OF MOLECULAR ORGANIZATION IN
BIOLOGICAL STRUCTURES USING A FULL WAVE PLATE COMPENSATOR 148 10.
DIFFERENTIAL INTERFERENCE CONTRAST (DIC) MICROSCOPY AND MODULATION
CONTRAST MICROSCOPY 153 OVERVIEW 153 THE DIC OPTICAL SYSTEM 153 DIC
EQUIPMENT AND OPTICS 155 THE DIC PRISM 157 DEMONSTRATION: THE ACTION OF
A WOLLASTON PRISM IN POLARIZED LIGHT 158 VIII CONTENTS FORMATION OF THE
DIC IMAGE 159 INTERFERENCE BETWEEN O AND E WAVEFRONTS AND THE
APPLICATION OF BIAS RETARDATION 160 ALIGNMENT OF DIC COMPONENTS 161
IMAGE INTERPRETATION 166 THE USE OF COMPENSATORS IN DIC MICROSCOPY 167
COMPARISON OF DIC AND PHASE CONTRAST OPTICS 168 MODULATION CONTRAST
MICROSCOPY 168 CONTRAST METHODS USING OBLIQUE ILLUMINATION 169 ALIGNMENT
OF THE MODULATION CONTRAST MICROSCOPE 172 EXERCISE: DIC MICROSCOPY 173
11. FLUORESCENCE MICROSCOPY 177 OVERVIEW 177 APPLICATIONS OF
FLUORESCENCE MICROSCOPY 178 PHYSICAL BASIS OF FLUORESCENCE 179
PROPERTIES OF FLUORESCENT DYES 182 DEMONSTRATION: FLUORESCENCE OF
CHLOROPHYLL AND FLUORESCEIN 183 AUTOFLUORESCENCE OF ENDOGENOUS MOLECULES
185 DEMONSTRATION: FLUORESCENCE OF BIOLOGICAL MATERIALS UNDER
ULTRAVIOLET LIGHT 189 ARRANGEMENT OF FILTERS AND THE EPI-ILLUMINATOR IN
THE FLUORESCENCE MICROSCOPE 189 OBJECTIVE LENSES AND SPATIAL RESOLUTION
IN FLUORESCENCE MICROSCOPY 194 CAUSES OF HIGH-FLUORESCENCE BACKGROUND
196 THE PROBLEM OF BLEED-THROUGH WITH MULTIPLY STAINED SPECIMENS 197
EXAMINING FLUORESCENT MOLECULES IN LIVING CELLS 198 EXERCISE:
FLUORESCENCE MICROSCOPY OF LIVING TISSUE CULTURE CELLS 199 12. CONFOCAL
LASER SCANNING MICROSCOPY 205 OVERVIEW * 205 THE OPTICAL PRINCIPLE OF
CONFOCAL IMAGING 208 DEMONSTRATION: ISOLATION OF FOCAL PLANE SIGNALS
WITH A CONFOCAL PINHOLE 211 ADVANTAGES OF CLSM OVER WIDE-FIELD
FLUORESCENCE SYSTEMS 213 CRITERIA DEFINING IMAGE QUALITY AND THE
PERFORMANCE OF AN ELECTRONIC IMAGING SYSTEM 215 ELECTRONIC ADJUSTMENTS
AND CONSIDERATIONS FOR CONFOCAL FLUORESCENCE IMAGING 217 PHOTOBLEACHING
223 GENERAL PROCEDURE FOR ACQUIRING A CONFOCAL IMAGE 224 TWO-PHOTON AND
MULTI-PHOTON LASER SCANNING MICROSCOPY 226 CONFOCAL IMAGING WITH A
SPINNING NIPKOW DISK 229 EXERCISE: EFFECT OF CONFOCAL VARIABLES ON IMAGE
QUALITY 230 13. VIDEO MICROSCOPY 233 OVERVIEW 233 APPLICATIONS AND
SPECIMENS SUITABLE FOR VIDEO 233 CONTENTS IX CONFIGURATION OF A VIDEO
CAMERA SYSTEM 234 TYPES OF VIDEO CAMERAS 236 ELECTRONIC CAMERA CONTROLS
238 DEMONSTRATION: PROCEDURE FOR ADJUSTING THE LIGHT INTENSITY OF THE
VIDEO CAMERA AND TV MONITOR 241 VIDEO ENHANCEMENT OF IMAGE CONTRAST 242
CRITERIA USED TO DEFINE VIDEO IMAGING PERFORMANCE 245 ALIASING 249
DIGITAL IMAGE PROCESSORS 249 IMAGE INTENSIFIERS 250 VCRS 251 SYSTEMS
ANALYSIS OF A VIDEO IMAGING SYSTEM 252 DAISY CHAINING A NUMBER OF
SIGNAL-HANDLING DEVICES 254 EXERCISE: CONTRAST ADJUSTMENT AND TIME-LAPSE
RECORDING WITH A VIDEO CAMERA 255 14. DIGITAL CCD MICROSCOPY 259
OVERVIEW 259 THE CHARGE-COUPLED DEVICE (CCD IMAGER) 260 CCD
ARCHITECTURES 267 NOTE: INTERLINE CCDSFOR BIOMEDICAL IMAGING 268
ANALOGUE AND DIGITAL CCD CAMERAS 269 CAMERA ACQUISITION PARAMETERS
AFFECTING CCD READOUT AND IMAGE QUALITY 269 IMAGING PERFORMANCE OF A CCD
DETECTOR 271 BENEFITS OF DIGITAL CCD CAMERAS 276 REQUIREMENTS AND
DEMANDS OF DIGITAL CCD IMAGING 276 COLOR CAMERAS^; 277 POINTS TO
CONSIDER WHEN CHOOSING A CAMERA 278 EXERCISE: EVALUATING THE PERFORMANCE
OF A CCD CAMERA 279 15. DIGITAL IMAGE PROCESSING 283 OVERVIEW 283
PRELIMINARIES: IMAGE DISPLAY AND DATA TYPES 284 HISTOGRAM ADJUSTMENT 285
ADJUSTING GAMMA (Y) TO CREATE EXPONENTIAL LUTS 287 FLAT-FIELD CORRECTION
289 IMAGE PROCESSING WITH FILTERS 292 SIGNAL-TO-NOISE RATIO 299
EXERCISE: FLAT-FIELD CORRECTION AND DETERMINATION OF S/N RATIO 305 16.
IMAGE PROCESSING FOR SCIENTIFIC PUBLICATION 307 OVERVIEW 307 IMAGE
PROCESSING: ONE VARIABLE OUT OF MANY AFFECTING THE APPEARANCE OF THE
MICROSCOPE IMAGE 307 THE NEED FOR IMAGE PROCESSING 309 CONTENTS VARYING
PROCESSING STANDARDS 309 RECORD KEEPING DURING IMAGE ACQUISITION AND
PROCESSING 310 NOTE: GUIDELINES FOR IMAGE ACQUISITION AND PROCESSING 310
USE OF COLOR IN PRINTS AND IMAGE DISPLAYS 312 COLOCALIZATION OF TWO
SIGNALS USING PSEUDOCOLOR 313 A CHECKLIST FOR EVALUATING IMAGE QUALITY
315 APPENDIX I 317 APPENDIX II 321 APPENDIX III 329 GLOSSARY 331
REFERENCES 357 INDEX 361
|
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| discipline | Allgemeine Naturwissenschaft Physik Biologie |
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| indexdate | 2024-07-09T19:02:03Z |
| institution | BVB |
| isbn | 047125391X |
| language | English |
| lccn | 2001024021 |
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| physical | XII, 368 S. zahlr. Ill. u. graph. Darst. |
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| publisher | Wiley-Liss |
| record_format | marc |
| spelling | Murphy, Douglas B. Verfasser aut Fundamentals of light microscopy and electronic imaging Douglas B. Murphy New York Wiley-Liss 2001 XII, 368 S. zahlr. Ill. u. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Buiging (natuurkunde) gtt Computergraphics gtt Digitale technieken gtt Fluorescentie gtt Interferentie (golven) gtt Kleuren gtt Licht gtt Microscopia larpcal Microscopie Microscopie gtt Microscopie ram Traitement d'images ram Microscopy Mikroskopie (DE-588)4039238-7 gnd rswk-swf Bildverarbeitung (DE-588)4006684-8 gnd rswk-swf Bildgebendes Verfahren (DE-588)4006617-4 gnd rswk-swf Bildgebendes Verfahren (DE-588)4006617-4 s DE-188 Mikroskopie (DE-588)4039238-7 s Bildverarbeitung (DE-588)4006684-8 s 1\p DE-604 http://www.loc.gov/catdir/description/wiley034/2001024021.html Publisher description http://www.loc.gov/catdir/toc/onix05/2001024021.html Table of Contents HEBIS Datenaustausch Darmstadt application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009840949&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Murphy, Douglas B. Fundamentals of light microscopy and electronic imaging Buiging (natuurkunde) gtt Computergraphics gtt Digitale technieken gtt Fluorescentie gtt Interferentie (golven) gtt Kleuren gtt Licht gtt Microscopia larpcal Microscopie Microscopie gtt Microscopie ram Traitement d'images ram Microscopy Mikroskopie (DE-588)4039238-7 gnd Bildverarbeitung (DE-588)4006684-8 gnd Bildgebendes Verfahren (DE-588)4006617-4 gnd |
| subject_GND | (DE-588)4039238-7 (DE-588)4006684-8 (DE-588)4006617-4 |
| title | Fundamentals of light microscopy and electronic imaging |
| title_auth | Fundamentals of light microscopy and electronic imaging |
| title_exact_search | Fundamentals of light microscopy and electronic imaging |
| title_full | Fundamentals of light microscopy and electronic imaging Douglas B. Murphy |
| title_fullStr | Fundamentals of light microscopy and electronic imaging Douglas B. Murphy |
| title_full_unstemmed | Fundamentals of light microscopy and electronic imaging Douglas B. Murphy |
| title_short | Fundamentals of light microscopy and electronic imaging |
| title_sort | fundamentals of light microscopy and electronic imaging |
| topic | Buiging (natuurkunde) gtt Computergraphics gtt Digitale technieken gtt Fluorescentie gtt Interferentie (golven) gtt Kleuren gtt Licht gtt Microscopia larpcal Microscopie Microscopie gtt Microscopie ram Traitement d'images ram Microscopy Mikroskopie (DE-588)4039238-7 gnd Bildverarbeitung (DE-588)4006684-8 gnd Bildgebendes Verfahren (DE-588)4006617-4 gnd |
| topic_facet | Buiging (natuurkunde) Computergraphics Digitale technieken Fluorescentie Interferentie (golven) Kleuren Licht Microscopia Microscopie Traitement d'images Microscopy Mikroskopie Bildverarbeitung Bildgebendes Verfahren |
| url | http://www.loc.gov/catdir/description/wiley034/2001024021.html http://www.loc.gov/catdir/toc/onix05/2001024021.html http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009840949&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT murphydouglasb fundamentalsoflightmicroscopyandelectronicimaging |