Verfügbarkeit: Testing for genetic manipulation in plants

Testing for genetic manipulation in plants: with 16 tables

Gespeichert in:

Bibliographische Detailangaben
Format:	Buch
Sprache:	English
Veröffentlicht:	Berlin [u.a.] Springer 2002
Schriftenreihe:	Molecular methods of plant analysis 22
Schlagworte:	Transgene Pflanzen Genanalyse Aufsatzsammlung
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	XVII, 194 S. Ill., graph. Darst.
ISBN:	3540431535

Internformat

MARC


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245	1	0	\|a Testing for genetic manipulation in plants \|b with 16 tables \|c ed. by J. F. Jackson ...
264		1	\|a Berlin [u.a.] \|b Springer \|c 2002
300			\|a XVII, 194 S. \|b Ill., graph. Darst.
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490	1		\|a Molecular methods of plant analysis \|v 22
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Datensatz im Suchindex

_version_	1804129206749102080
adam_text	CONTENTS 1 SELECTABLE AND SCREENABLE MARKERS FOR RICE TRANSFORMATION R.M. TWYMAN, E. STOEGER, A. KOHLI, T. CAPELL, AND P. CHRISTOU 1.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1.2 DOMINANT SELECTABLE MARKERS FOR RICE . . . . . . . . . . . . . . . . . . . . . . . 3 1.2.1 AMINOGLYCOSIDE 3 -PHOSPHOTRANSFERASE (NEOMYCIN PHOSPHOTRANSFERASE) . . . . . . . . . . . . . . . . . . . . . . . 3 1.2.2 HYGROMYCIN PHOSPHOTRANSFERASE . . . . . . . . . . . . . . . . . . . . . 4 1.2.3 PHOSPHINOTHRICIN ACETYLTRANSFERASE . . . . . . . . . . . . . . . . . . . 4 1.2.4 OTHER DOMINANT SELECTABLE MARKERS . . . . . . . . . . . . . . . . . . . 5 1.3 NOVEL SELECTABLE MARKERS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 1.3.1 INNOCUOUS MARKERS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 1.3.2 COUNTERSELECTABLE MARKERS . . . . . . . . . . . . . . . . . . . . . . . . . . 7 1.4 SCREENABLE MARKER GENES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 1.4.1 B -GLUCURONIDASE ( GUSA ) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 1.4.2 FIREFLY LUCIFERASE ( LUC ) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 1.4.3 GREEN FLUORESCENT PROTEIN ( GFP ) . . . . . . . . . . . . . . . . . . . . . . 11 1.5 STRATEGIES FOR MARKER-GENE DELIVERY . . . . . . . . . . . . . . . . . . . . . . . . 13 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 2 USE OF GREEN FLUORESCENT PROTEIN TO DETECT TRANSFORMED SHOOTS J. MOLINIER AND G. HAHNE 2.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 2.2 GFP: SUITABLE AS A VISUALLY SELECTABLE MARKER IN PLANTA ? . . . . . . . . 20 2.2.1 IMPORTANT PROPERTIES OF THE PROTEIN . . . . . . . . . . . . . . . . . . . 20 2.2.2 PROPERTIES OF A USEFUL SELECTABLE MARKER IN PLANT TRANSFORMATION TECHNOLOGY . . . . . . . . . . . . . . . . . . . . . . . . . 21 2.3 GFP EXPRESSION AND DETECTION IN PRIMARY TRANSFORMED TISSUES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 2.3.1 TRANSIENT EXPRESSION AND GFP DETECTION . . . . . . . . . . . . . . . 22 2.3.2 DETECTION EQUIPMENT AND TROUBLESHOOTING . . . . . . . . . . . . . 22 2.3.3 STABLE EXPRESSION AND GFP DETECTION IN PRIMARY TRANSFORMED TISSUES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 2.4 GFP FOR SCREENING OF SEGREGATING POPULATIONS . . . . . . . . . . . . . . . . 28 2.5 CONCLUSION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 3 LUCIFERASE GENE EXPRESSED IN PLANTS, NOT IN AGROBACTERIUM S.L. MANKIN 3.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 3.2 PREVENTING BACTERIAL EXPRESSION . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 3.3 IMAGING LUCIFERASE ACTIVITY IN PLANTA . . . . . . . . . . . . . . . . . . . . . . . 32 3.4 MEASURING LUCIFERASE ACTIVITY IN PLANT EXTRACTS . . . . . . . . . . . . . . . 34 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 4 USE OF B -GLUCURONIDASE (GUS) TO SHOW DEHYDRATION AND HIGH-SALT GENE EXPRESSION K. NAKASHIMA AND K. YAMAGUCHI-SHINOZAKI 4.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 4.2 WHAT IS GUS? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 4.3 TRASGENIC PLANTS CARRYING PROMOTER- GUS CONSTRUCTS . . . . . . . . . . . 41 4.3.1 CONSTRUCTION OF PROMOTER- GUS FUSION GENES . . . . . . . . . . . 44 4.3.2 INTRODUCTION OF PROMOTER- GUS CONSTRUCTS INTO AGROBACTERIUM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 4.3.3 TRANSFORMATION OF PLANTS WITH AGROBACTERIUM . . . . . . . . . . . 46 4.3.3.1 TRANSFORMATION OF ARABIDOPSIS PLANTS . . . . . . . . . . . 46 4.4 FLUOROMETRIC ASSAY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 4.4.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 4.4.2 STRESS CONDITIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 4.4.2.1 PLANT PREPARATION . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 4.4.2.2 DEHYDRATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 4.4.2.3 HIGH SALINITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 4.4.2.4 ABA TREATMENT . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 4.4.2.5 OTHER TREATMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 4.4.3 PROTEIN ASSAY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 4.4.4 SAMPLE PREPARATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 4.4.5 FLUOROMETRIC ASSAY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 4.5 HISTOCHEMISTRY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 4.5.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 4.5.2 HISTOCHEMISTRY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 4.6 NORTHERN ANALYSIS OF GUS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 4.6.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 4.6.2 RNA EXTRACTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 4.6.3 RNA BLOTTING . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 4.6.4 NORTHERN HYBRIDIZATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 4.7 APPLICATION OF THE GUS SYSTEM . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 4.7.1 TRANSIENT ASSAY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 4.7.2 TRANSACTIVATION EXPERIMENT . . . . . . . . . . . . . . . . . . . . . . . . . 57 4.7.3 PROMOTER TAGGING (ENHANCER TRAP) . . . . . . . . . . . . . . . . . . . 57 4.8 CONCLUSION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59 X CONTENTS 5 METHODS FOR DETECTING GENETIC MANIPULATION IN GRAIN LEGUMES H.-J. JACOBSEN AND R. GREINER 5.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 5.2 DETECTION AT THE DNA LEVEL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 5.3 PCR ANALYSIS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 5.4 CONTROL PCR AND SPECIFIC PCR SYSTEMS . . . . . . . . . . . . . . . . . . . . . . 66 5.5 QUANTITATIVE APPROACH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69 5.6 COMPETITIVE PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69 5.7 REAL-TIME PCR SYSTEMS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70 5.8 CONCLUDING REMARKS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71 6 ELIMINATION OF SELECTABLE MARKER GENES FROM TRANSGENIC CROPS A.P. GLEAVE 6.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 6.2 CO-TRANSFORMATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74 6.3 TRANSPOSON-MEDIATED APPROACHES . . . . . . . . . . . . . . . . . . . . . . . . . . 78 6.3.1 TRANSPOSON-MEDIATED REPOSITIONING . . . . . . . . . . . . . . . . . . 78 6.3.2 TRANSPOSON-MEDIATED ELIMINATION . . . . . . . . . . . . . . . . . . . . 80 6.4 SITE-SPECIFIC RECOMBINATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 6.4.1 THE CRE/ LOX P SYSTEM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82 6.4.2 THE FLP/ FRT SYSTEM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86 6.4.3 THE R /RS SYSTEM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88 6.5 INTRACHROMOSOMAL HOMOLOGOUS RECOMBINATION . . . . . . . . . . . . . . . 89 6.6 CONCLUSIONS AND FUTURE PROSPECTS . . . . . . . . . . . . . . . . . . . . . . . . . . 90 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 7 GST-MAT VECTOR FOR THE EFFICIENT AND PRACTICAL REMOVAL OF MARKER GENES FROM TRANSGENIC PLANTS H. EBINUMA, K. SUGITA, E. MATSUNAGA, S. ENDO, AND K. YAMADA 7.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95 7.2 IPT -TYPE MAT VECTORS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96 7.2.1 TRANSPOSABLE ELEMENT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96 7.2.2 SITE-SPECIFIC RECOMBINATION SYSTEM . . . . . . . . . . . . . . . . . . . 97 7.2.3 ADVANTAGES OF THE IPT GENE . . . . . . . . . . . . . . . . . . . . . . . . . . 101 7.3 TWO-STEP TRANSFORMATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103 7.3.1 PROMOTER OF THE R GENE . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103 7.3.2 PROMOTER OF THE IPT GENE . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105 7.3.3 COMBINATION OF THE IPT AND IAA M/H GENES . . . . . . . . . . . . . 108 7.3.4 TRANSGENE STACKING . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 7.4 SINGLE-STEP TRANSFORMATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112 CONTENTS XI 7.5 CLONING VECTOR FOR DESIRED GENES . . . . . . . . . . . . . . . . . . . . . . . . . . 114 7.6 CONCLUDING REMARKS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116 8 SAFETY ASSESSMENT OF INSECT PROTECTED CROPS: TESTING THE FEEDING VALUE OF BT CORN AND COTTON VARIETIES IN POULTRY, SWINE AND CATTLE B. HAMMOND, E. STANISIEWSKI, R. FUCHS, J. ASTWOOD, AND G. HARTNELL 8.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119 8.1.1 FOOD SAFETY STANDARDS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119 8.1.2 TESTING FOR FOOD AND FEED SAFETY . . . . . . . . . . . . . . . . . . . . . 120 8.2 INSECT PROTECTION TRAITS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122 8.3 BENEFITS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123 8.4 SAFETY ASSESSMENT OF THE CRY INSECT-CONTROL PROTEINS . . . . . . . . . . . 124 8.5 MODE OF ACTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125 8.6 SUBSTANTIAL EQUIVALENCE BASED ON COMPOSITIONAL ANALYSIS . . . . . . . 126 8.7 CURRENT PRODUCTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127 8.8 GROWER ACCEPTANCE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127 8.9 FUTURE PRODUCTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127 8.10 FARM-ANIMAL STUDIES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128 8.10.1 BT CORN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128 8.10.1.1 POULTRY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128 8.10.1.2 LACTATING COWS . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131 8.10.1.3 BEEF AND SHEEP . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131 8.10.1.4 SWINE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132 8.11 COTTONSEED . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133 8.12 CONCLUSIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135 9 SAFETY ASSESSMENT OF GENETICALLY MODIFIED RICE AND POTATOES WITH SOYBEAN GLYCININ K. MOMMA, W. HASHIMOTO, S. UTSUMI, AND K. MURATA 9.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 9.2 SAFETY ASSESSMENT OF GENETICALLY MODIFIED CROPS . . . . . . . . . . . . . . 140 9.2.1 GENETICALLY MODIFIED RICE . . . . . . . . . . . . . . . . . . . . . . . . . . . 141 9.2.2 GENETICALLY MODIFIED POTATOES . . . . . . . . . . . . . . . . . . . . . . . . 144 9.3 CONCLUDING REMARKS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 10 CHROMOSOMAL AND GENETIC ABERRATIONS IN TRANSGENIC SOYBEAN R.J. SINGH 10.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153 10.2 TIMES IN CULTURE WITH 2,4-D PRIOR TO TRANSFORMATION . . . . . . . . . . . 154 10.3 GENETIC BACKGROUND OF THE EXPLANTS . . . . . . . . . . . . . . . . . . . . . . . . 159 XII CONTENTS 10.4 SEED FERTILITY IN TRANSGENIC SOYBEAN . . . . . . . . . . . . . . . . . . . . . . . . 160 10.5 CYTOLOGICAL BASIS OF GENE SILENCING . . . . . . . . . . . . . . . . . . . . . . . . . 163 10.6 CONCLUSIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 164 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165 11 TRANSGENIC BARLEY ( HORDEUM VULGARE L.) AND CHROMOSOMAL VARIATION M.-J. CHO, H.W. CHOI, P. BREGITZER, S. ZHANG, AND P.G. LEMAUX 11.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169 11.2 CHROMOSOMAL VARIATION IN NONTRANSGENIC BARLEY PLANTS . . . . . . . . 170 11.3 CHROMOSOMAL VARIATION IN TRANSGENIC BARLEY PLANTS . . . . . . . . . . . 172 11.4 FIDELITY AND QUALITY OF TRANSGENIC BARLEY PLANTS . . . . . . . . . . . . . . . 177 11.4.1 COMPARATIVE ANALYSIS OF GENOMIC STABILITY IN PLANTS DERIVED FROM TISSUES GENERATED USING DIFFERENT IN VITRO PROLIFERATION PROCESSES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177 11.4.2 SOMACLONAL VARIATION AND FIELD PERFORMANCE OF TRANSGENIC PLANTS DERIVED FROM EMBRYOGENIC CALLUS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179 11.4.3 STABILITY OF TRANSGENES AND TRANSGENE EXPRESSION . . . . . . . . 180 11.5 CONCLUSIONS AND FUTURE PERSPECTIVES . . . . . . . . . . . . . . . . . . . . . . . . 184 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185 SUBJECT INDEX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 189 CONTENTS XIII
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isbn	3540431535
language	English
oai_aleph_id	oai:aleph.bib-bvb.de:BVB01-009816607
oclc_num	248454719
open_access_boolean
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physical	XVII, 194 S. Ill., graph. Darst.
publishDate	2002
publishDateSearch	2002
publishDateSort	2002
publisher	Springer
record_format	marc
series	Molecular methods of plant analysis
series2	Molecular methods of plant analysis
spelling	Testing for genetic manipulation in plants with 16 tables ed. by J. F. Jackson ... Berlin [u.a.] Springer 2002 XVII, 194 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Molecular methods of plant analysis 22 Transgene Pflanzen (DE-588)4311513-5 gnd rswk-swf Genanalyse (DE-588)4200230-8 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Transgene Pflanzen (DE-588)4311513-5 s Genanalyse (DE-588)4200230-8 s DE-604 Jackson, John F. 1935- Sonstige (DE-588)131937375 oth Molecular methods of plant analysis 22 (DE-604)BV035419634 22 SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009816607&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Testing for genetic manipulation in plants with 16 tables Molecular methods of plant analysis Transgene Pflanzen (DE-588)4311513-5 gnd Genanalyse (DE-588)4200230-8 gnd
subject_GND	(DE-588)4311513-5 (DE-588)4200230-8 (DE-588)4143413-4
title	Testing for genetic manipulation in plants with 16 tables
title_auth	Testing for genetic manipulation in plants with 16 tables
title_exact_search	Testing for genetic manipulation in plants with 16 tables
title_full	Testing for genetic manipulation in plants with 16 tables ed. by J. F. Jackson ...
title_fullStr	Testing for genetic manipulation in plants with 16 tables ed. by J. F. Jackson ...
title_full_unstemmed	Testing for genetic manipulation in plants with 16 tables ed. by J. F. Jackson ...
title_short	Testing for genetic manipulation in plants
title_sort	testing for genetic manipulation in plants with 16 tables
title_sub	with 16 tables
topic	Transgene Pflanzen (DE-588)4311513-5 gnd Genanalyse (DE-588)4200230-8 gnd
topic_facet	Transgene Pflanzen Genanalyse Aufsatzsammlung
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009816607&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
volume_link	(DE-604)BV035419634
work_keys_str_mv	AT jacksonjohnf testingforgeneticmanipulationinplantswith16tables

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