Macrophages: a practical approach
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Oxford [u.a.]
Oxford Univ. Press
2000
|
| Edition: | 1. publ. |
| Series: | The practical approach series
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XVI, 211 S. Ill., graph. Darst. |
| ISBN: | 0199636893 0199636885 |
Staff View
MARC
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| 245 | 1 | 0 | |a Macrophages |b a practical approach |c ed. by Donna M. Paulnock |
| 250 | |a 1. publ. | ||
| 264 | 1 | |a Oxford [u.a.] |b Oxford Univ. Press |c 2000 | |
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Record in the Search Index
| _version_ | 1804129030008471552 |
|---|---|
| adam_text | Macrophages
^ Prated©®! ^pproasllt
Edited by
Donna M Paulnock
Department of Medical Microbiology and
Immunology, University of Wisconsin Medical
School, USA
OXFORD
UNIVERSITY PRESS
Contents
Preface page v
List of protocols xi
Abbreviations xv
1 Isolation of macrophages from tissues, fluids, and immune response
sites 1
Mary Ellen Handel-Fernandez and Diana M Lopez
1 Introduction l
2 The heterogeneity of macrophages l
3 Isolation of free mononuclear phagocytes 2
Murine peritoneal cells 2
Blood monocytes 6
Alveolar macrophages 7
4 Macrophages in haematopoietic tissues 10
5 Fixed tissue macrophages 14
Mechanical and enzymatic digestion of tissue 15
6 Macrophages in immune response sites 25
Macrophages in infection 25
^ Tumour-associated macrophages 26
References 28
2 Purification of macrophages 31
Sandra Gessani, Laura Fantuzzi, Patrizia Puddu, and Filippo Belardelli
1 Introduction 31
2 Purification of macrophages by adherence-based methods 32
Adhesion properties of macrophages 32
Macrophage adhesion molecules 33
Effect of adherence on macrophage gene expression 33
Effect of adherence on macrophage functional activities 35
3 Technical approaches for macrophage purification by adherence-based
methods 35
Adherence to uncoated plastic or glass surfaces 36
CONTENTS
Adherence to gelatin-coated surfaces 36
Adherence to microexudate-coated surfaces 38
Adherence to collagen matrices 39
4 Methods for detachment of adherent macrophages 40
Mechanical detachment 40
Recovery of adherent cells by EDTA treatment 41
Recovery of adherent macrophages by lignocaine treatment 42
5 General considerations on the adherence-based methods 43
Effects of different surfaces on macrophage morphology and functions 43
Effects of different detachment procedures on macrophage physiology 44
6 Physical methods of macrophage purification 45
Purification of macrophages by isopycnic gradient centrifugation 46
Isolation of whole mononuclear cells from peripheral blood by the
Ficoll-Hypaque gradient 46
Purification of monocytes by Percoll gradient 48
Importance of the control of experimental conditions in the preparation
of a gradient 49
Counterflow centrifugal elutriation 50
7 Additional issues to be considered in monocytes/macrophages
purification 54
Macrophage heterogeneity 54
Comparison of the efficacy of different purification techniques with
respect to the source of macrophages 55
Non-adherent versus adherent culture of macrophages 56
Problems caused by LPS contamination during the course of macrophage
purification and culture 56
8 Conclusions 56
Acknowledgements 57
References 57
3 Characterization of macrophage antigens and receptors by
immunochemistry and fluorescent analysis: expression,
endocytosis, and phagocytosis 6i
Leanne Peiser, Peter J Gough, Elizabeth Darley, and Siamon Gordon
1 Introduction 61
2 Immunochemical labelling of monocytes and macrophages 62
Introduction 62
Practical considerations for immunofluorescent staining of macrophage
populations in vitro 64
Preparation of in vitro macrophage cultures for immunochemical staining 65
3 Detection techniques for fluorescent analysis of macrophages 70
Flow cytometry 70
Fluorescent microscopy 71
4 Immunohistochemical staining of macrophages in mouse tissues 71
Preparation of tissue 72
Immunochemical staining of tissue 76
5 Fluorescent analysis of macrophage endocytic function 78
Introduction to the endocytic pathway 78
Practical considerations for testing macrophage endocytic function 81
Practical considerations for testing macrophage phagocytic function 86
CONTENTS
6 Conclusion 89
Acknowledgements 90
References 90
4 Analysis of antigen processing and presentation 93
P M Kaye
1 Introduction 93
2 Preliminary considerations in study design 93
A definition of antigen processing and presentation 93
T cell choice restricts functional interpretation 94
In vivo Veritas 95
The dendritic cell issue 95
Pathogens are not simple antigens 96
3 Analysis of class I and II antigen processing 96
Pathways of processing 96
Modified protocols for use with pathogens 105
Cell biology of antigen processing 110
4 Analysing antigen presenting function of macrophages 110
Correlative studies of cell phenotype 110
Functional assays of co-stimulation 111
References 112
5 Macrophage secretory products l i s
Paola ARavena, Giancarlo Bianchi, Walter Luini, Andrea Doni, Pietro Transidico,
Sflvano Sozzani, and Alberto Mantovani
1 Introduction 115
2 Cytokines and chemokines: chemotaxis 116
3 Leukocyte transmigration 118
4 Reverse transmigration 121
5 Soluble cytokine receptors 122
6 Cross-linking of soluble receptors 124
References 125 _
6 Analysis of macrophage lytic functions 127
Maria Carla Bosco, Tiziana Musso, Luca Carta, and Luigi Varesio
1 Introduction 127
Biological perspective 127
Technical notes 129
2 Macrophage-mediated anti-tumour activity 130
Morphological tumour cell counting assay 130
Macrophage-mediated cytolysis: release of radioisotopes 133
Macrophage-mediated cytostasis of tumour cells: incorporation of radioactive
labels 140
3 Target cell sensitivity 141
ix
CONTENTS
4 Microbicidal activity 142
Anti-Lefchmama activity of monocytes/macrophages 144
Anti-fungal activity 147
References 153
7 Analysis of macrophage activity In vivo 157
Nico van Rooijen and Esther van Kesteren-Hendrikx
1 Introduction 157
2 Previous methods for blocking of phagocytosis 157
Silica, carrageenan, and dextran sulfate 157
Gadolinium chloride 158
Anti-macrophage antibodies and receptor antagonists 159
Competition 159
3 The liposome-mediated macrophage suicide technique 160
Principles 160
Liposomes 161
Liposome-encapsulated clodronate 162
Injection of liposomes and access to tissue macrophages 165
Selectivity of the approach with respect to macrophages 166
Duration of macrophage depletion 167
4 Practical applications of the technique 168
Improved efficacy of carrier-mediated gene transfer 168
Improved survival of human cells in immunodeficient (SCID) mice 169
Suppression of inflammatory reactions 170
Improved graft survival and functioning 170
References 171
8 Analysis of gene expression in mononuclear phagocytes 173
Joyce E S Doan, Thomas A Hamilton, and Donna M Paulnock
1 Introduction 173
2 Detection and quantification of specific RNA levels 174
Basic principles 174
Preparation of total cellular RNA 174
Nuclear run-on analysis 177
Quantification-of specific mRNAs 181
3 Gene transfer 185 ~
Basic principles 185
Experimental strategies 185
Transient transfection 186
Stable transfection 192
4 Measurement of protein-nucleic acid interactions 193
Basic principles 193
Sources and characteristics of binding factors 194
Measurements of DNA and RNA binding proteins 195
References 201
A1 List of suppliers 203
Index 209
xt
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| discipline | Biologie Medizin |
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| physical | XVI, 211 S. Ill., graph. Darst. |
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| series2 | The practical approach series |
| spelling | Macrophages a practical approach ed. by Donna M. Paulnock 1. publ. Oxford [u.a.] Oxford Univ. Press 2000 XVI, 211 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier The practical approach series Macrophages - Recherche - Méthodologie Immunologic Techniques Laboratory Manuals Macrophages Macrophages Laboratory Manuals Macrophages Laboratory manuals Biochemie (DE-588)4006777-4 gnd rswk-swf Makrophage (DE-588)4193041-1 gnd rswk-swf Makrophage (DE-588)4193041-1 s Biochemie (DE-588)4006777-4 s DE-604 Paulnock, Donna M. Sonstige oth HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009701281&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Macrophages a practical approach Macrophages - Recherche - Méthodologie Immunologic Techniques Laboratory Manuals Macrophages Macrophages Laboratory Manuals Macrophages Laboratory manuals Biochemie (DE-588)4006777-4 gnd Makrophage (DE-588)4193041-1 gnd |
| subject_GND | (DE-588)4006777-4 (DE-588)4193041-1 |
| title | Macrophages a practical approach |
| title_auth | Macrophages a practical approach |
| title_exact_search | Macrophages a practical approach |
| title_full | Macrophages a practical approach ed. by Donna M. Paulnock |
| title_fullStr | Macrophages a practical approach ed. by Donna M. Paulnock |
| title_full_unstemmed | Macrophages a practical approach ed. by Donna M. Paulnock |
| title_short | Macrophages |
| title_sort | macrophages a practical approach |
| title_sub | a practical approach |
| topic | Macrophages - Recherche - Méthodologie Immunologic Techniques Laboratory Manuals Macrophages Macrophages Laboratory Manuals Macrophages Laboratory manuals Biochemie (DE-588)4006777-4 gnd Makrophage (DE-588)4193041-1 gnd |
| topic_facet | Macrophages - Recherche - Méthodologie Immunologic Techniques Laboratory Manuals Macrophages Macrophages Laboratory Manuals Macrophages Laboratory manuals Biochemie Makrophage |
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