The interaction of conotoxins with voltage gated ion channels: influence of membrane potential and ionic milieu on the binding of Kappa-conotoxin PVIIA to Shaker K+-channnels
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Göttingen
Cuvillier
2001
|
| Edition: | 1. Aufl. |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Zugl.: München, Techn. Univ., Diss., 2001 |
| Physical Description: | 90, V S. Ill., graph. Darst. |
| ISBN: | 3898732983 |
Staff View
MARC
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| 001 | BV014096411 | ||
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| 008 | 020115s2001 gw ad|| m||| 00||| eng d | ||
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| 020 | |a 3898732983 |9 3-89873-298-3 | ||
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| 100 | 1 | |a Boccaccio, Anna Elisabetta |d 1971- |e Verfasser |0 (DE-588)123467217 |4 aut | |
| 245 | 1 | 0 | |a The interaction of conotoxins with voltage gated ion channels |b influence of membrane potential and ionic milieu on the binding of Kappa-conotoxin PVIIA to Shaker K+-channnels |c Anna Elisabetta Boccaccio |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a Göttingen |b Cuvillier |c 2001 | |
| 300 | |a 90, V S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Zugl.: München, Techn. Univ., Diss., 2001 | ||
| 650 | 0 | 7 | |a Genprodukt |0 (DE-588)4330422-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Conotoxine |0 (DE-588)4717814-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Wechselwirkung |0 (DE-588)4064937-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Gen shaker |0 (DE-588)4335800-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Kaliumkanal |0 (DE-588)4258505-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Spannungskontrollierter Ionenkanal |0 (DE-588)4717815-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Bindeproteine |0 (DE-588)4276933-4 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
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| 689 | 0 | 1 | |a Genprodukt |0 (DE-588)4330422-9 |D s |
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| 689 | 1 | 2 | |a Bindeproteine |0 (DE-588)4276933-4 |D s |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009657391 | |
Record in the Search Index
| _version_ | 1816444669490364416 |
|---|---|
| adam_text |
CONTENTS
1
INTRODUCTION
1
1.1
I
ON
CHANNELS
:
FOCUS
ON
THE
VOLTAGE
-
GATED
S
HAKEK
K
+
CHANNELS
.
1
1.2
CONOTOXINS
AND
K-PVIIA,
A
K
+
CHANNEL
BLOCKER
.
5
2
MATERIALS
AND
METHODS
_
YY
_
1
2.1
OOCYTES
SOLUTIONS
.
9
2.2
P
REPARATION
OF
OOCYTES
.
10
2.3
E
LECTROPHYSIOLOGICAL
METHODS
.
12
23.1
TWO-ELECTRODE
VOLTAGE
CLAMP
(TEVC)
.
13
232
PATCH
CLAMP
.
14
2.4
I
NSTR
UMENTATION
.
15
2.4.1
MECHANICAL
SET-UP
AND
ELECTRICAL
RECORDINGS
.
15
2.42
MICROPIPETTES
AND
ELECTRODES
.
.
,16
2.5
S
TIMULATION
PROTOCOLS
AND
DATA
ANALYSIS
.
17
2
5.1
IV-PROTOCOL
.
18
252
VARIABLE
PREPULSE
DURATION
TAIL
PROTOCOL
.
19
253
DOUBLE
PULSE
PROTOCOL
.
20
25.4
DATA
ANALYSIS
.
20
255
LEAKAGE
.
20
2.6
K
INETIC
ANALYSIS
.
21
3
RESULTS
_
23
3.1
K
-PVIIA
BLOCK
IN
EXTRACELLULAR
NFR
.23
3.1.1
SHAKER-WT
.
23
3.1.2
SHAKER-M-46
.
25
3.1.3
CLOSED-CHANNEL
BLOCK:
SHAKER-M-46
.
29
3.1.4
CLOSED-CHANNEL
BLOCK
IN
NFR:
SHAKER-WT
.
31
32
E
FFECT
OF
[KJO
ON
THE
BINDING
OF
K
-PVIIA
.
31
32.1
SHAKER-WT
.
31
322
SHAKER-M-46:
OPEN
CHANNEL
BLOCK
.
32
323
SHAKER-M-46:
CLOSED
CHANNEL
BLOCK
.
34
3.2.4
CLOSED
CHANNEL
BLOCK
AS
A
FUNCTION
OF[K]Q.
.
.36
3.3
B
INDING
OF
K
-PVIIA
IN
ABSENCE
OF
EXTERNAL
K
+
.
38
3.4
E
FFECT
OF
[R
B
]
Q
ON
THE
BINDING
OF
K
-PVHA
.
39
3.4.1115
INTKCL,
115
EXTRBCL
.
40
3.5
E
FFECT
OF
INTRACELLULAR
CATIONS
.
46
35.1
OPEN
CHANNEL
BLOCK
.
46
352
CLOSED
CHANNEL
BLOCK
.
54
35.3
INTERMEDIATE
[K]J
.
55
3.6
E
FFECTS
OF
TOXIN
BINDING
ON
"
TAIL
CURRENTS
"
.57
3.7
E
FFECT
OF
SYMMETRICAL
[R
B
)
O
.
58
4
DISCUSSION
_61
4.1
K
-PVIIA
IS
A
PORE
BLOCKER
.
61
42
K-PVIIA-BLOCK
IS
STATE-DEPENDENT
.
61
43
K-PVIIA
BLOCK
OF
OPEN
CHANNELS
IS
VOLTAGE
DEPENDENT
.
62
4.4
A
NALOGY
BETWEEN
K
-PVIIA
AND
CTX
.
63
4.5
A
MODEL
FOR
THE
STATE-DEPENDENCY
OF
K-PVIIA-BLOCK
.
66
4.5.1
DEPENDENCE
OF
THE
OPEN
CHANNEL
BLOCK
ON
THE
SOLUTION
COMPOSITION
.
67
45.2
CLOSED
CHANNEL
BLOCK
VS
[KJO
.
72
453
CLOSED
CHANNEL
BLOCK
DOES
NOT
DEPEND
ON
THE
INTRACELLULAR
SOLUTION
.
76
4.6
E
FFECTS
OFTOXIN
BINDINGON
"
TAIL
CURRENTS
"
.
76
4.7
K
-PVIIA
DESTABILIZATION
BY
R
B
+
.
78
4.7.1
CLOSED
CHANNEL
BLOCK
VS
[RB]Q
.
4.7.2
SIMULATION
OF
CLOSED
CHANNEL
BLOCKIN
[K)O
OR
[RB]O
.
87
4.7.3
OPEN
CHANNEL
BLOCK
VS
[RB]O
.
83
4.8
K
+
CHANNELS
HAVE
3
-
4
K
+
-BIND1NG
SITES
.
85
4.9
S
UMMARY
.89
ABBREVIATIONS
91
REFERENCES
_
I |
| any_adam_object | 1 |
| author | Boccaccio, Anna Elisabetta 1971- |
| author_GND | (DE-588)123467217 |
| author_facet | Boccaccio, Anna Elisabetta 1971- |
| author_role | aut |
| author_sort | Boccaccio, Anna Elisabetta 1971- |
| author_variant | a e b ae aeb |
| building | Verbundindex |
| bvnumber | BV014096411 |
| classification_tum | PHY 823d CHE 820d PHY 821d |
| ctrlnum | (OCoLC)237482601 (DE-599)BVBBV014096411 |
| discipline | Physik Biologie Chemie |
| edition | 1. Aufl. |
| format | Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV014096411 |
| illustrated | Illustrated |
| indexdate | 2024-11-22T17:30:26Z |
| institution | BVB |
| isbn | 3898732983 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009657391 |
| oclc_num | 237482601 |
| open_access_boolean | |
| owner | DE-91 DE-BY-TUM DE-12 |
| owner_facet | DE-91 DE-BY-TUM DE-12 |
| physical | 90, V S. Ill., graph. Darst. |
| publishDate | 2001 |
| publishDateSearch | 2001 |
| publishDateSort | 2001 |
| publisher | Cuvillier |
| record_format | marc |
| spelling | Boccaccio, Anna Elisabetta 1971- Verfasser (DE-588)123467217 aut The interaction of conotoxins with voltage gated ion channels influence of membrane potential and ionic milieu on the binding of Kappa-conotoxin PVIIA to Shaker K+-channnels Anna Elisabetta Boccaccio 1. Aufl. Göttingen Cuvillier 2001 90, V S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zugl.: München, Techn. Univ., Diss., 2001 Genprodukt (DE-588)4330422-9 gnd rswk-swf Conotoxine (DE-588)4717814-0 gnd rswk-swf Wechselwirkung (DE-588)4064937-4 gnd rswk-swf Gen shaker (DE-588)4335800-7 gnd rswk-swf Kaliumkanal (DE-588)4258505-3 gnd rswk-swf Spannungskontrollierter Ionenkanal (DE-588)4717815-2 gnd rswk-swf Bindeproteine (DE-588)4276933-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Gen shaker (DE-588)4335800-7 s Genprodukt (DE-588)4330422-9 s Bindeproteine (DE-588)4276933-4 s Wechselwirkung (DE-588)4064937-4 s DE-604 Spannungskontrollierter Ionenkanal (DE-588)4717815-2 s Kaliumkanal (DE-588)4258505-3 s Conotoxine (DE-588)4717814-0 s 1\p DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009657391&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Boccaccio, Anna Elisabetta 1971- The interaction of conotoxins with voltage gated ion channels influence of membrane potential and ionic milieu on the binding of Kappa-conotoxin PVIIA to Shaker K+-channnels Genprodukt (DE-588)4330422-9 gnd Conotoxine (DE-588)4717814-0 gnd Wechselwirkung (DE-588)4064937-4 gnd Gen shaker (DE-588)4335800-7 gnd Kaliumkanal (DE-588)4258505-3 gnd Spannungskontrollierter Ionenkanal (DE-588)4717815-2 gnd Bindeproteine (DE-588)4276933-4 gnd |
| subject_GND | (DE-588)4330422-9 (DE-588)4717814-0 (DE-588)4064937-4 (DE-588)4335800-7 (DE-588)4258505-3 (DE-588)4717815-2 (DE-588)4276933-4 (DE-588)4113937-9 |
| title | The interaction of conotoxins with voltage gated ion channels influence of membrane potential and ionic milieu on the binding of Kappa-conotoxin PVIIA to Shaker K+-channnels |
| title_auth | The interaction of conotoxins with voltage gated ion channels influence of membrane potential and ionic milieu on the binding of Kappa-conotoxin PVIIA to Shaker K+-channnels |
| title_exact_search | The interaction of conotoxins with voltage gated ion channels influence of membrane potential and ionic milieu on the binding of Kappa-conotoxin PVIIA to Shaker K+-channnels |
| title_full | The interaction of conotoxins with voltage gated ion channels influence of membrane potential and ionic milieu on the binding of Kappa-conotoxin PVIIA to Shaker K+-channnels Anna Elisabetta Boccaccio |
| title_fullStr | The interaction of conotoxins with voltage gated ion channels influence of membrane potential and ionic milieu on the binding of Kappa-conotoxin PVIIA to Shaker K+-channnels Anna Elisabetta Boccaccio |
| title_full_unstemmed | The interaction of conotoxins with voltage gated ion channels influence of membrane potential and ionic milieu on the binding of Kappa-conotoxin PVIIA to Shaker K+-channnels Anna Elisabetta Boccaccio |
| title_short | The interaction of conotoxins with voltage gated ion channels |
| title_sort | the interaction of conotoxins with voltage gated ion channels influence of membrane potential and ionic milieu on the binding of kappa conotoxin pviia to shaker k channnels |
| title_sub | influence of membrane potential and ionic milieu on the binding of Kappa-conotoxin PVIIA to Shaker K+-channnels |
| topic | Genprodukt (DE-588)4330422-9 gnd Conotoxine (DE-588)4717814-0 gnd Wechselwirkung (DE-588)4064937-4 gnd Gen shaker (DE-588)4335800-7 gnd Kaliumkanal (DE-588)4258505-3 gnd Spannungskontrollierter Ionenkanal (DE-588)4717815-2 gnd Bindeproteine (DE-588)4276933-4 gnd |
| topic_facet | Genprodukt Conotoxine Wechselwirkung Gen shaker Kaliumkanal Spannungskontrollierter Ionenkanal Bindeproteine Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009657391&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT boccaccioannaelisabetta theinteractionofconotoxinswithvoltagegatedionchannelsinfluenceofmembranepotentialandionicmilieuonthebindingofkappaconotoxinpviiatoshakerkchannnels |