Two-hybrid systems: methods and protocols
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2001
|
| Series: | Methods in molecular biology
177 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XII, 336 S. Ill., graph. Darst. |
| ISBN: | 0896038327 |
Staff View
MARC
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| 245 | 1 | 0 | |a Two-hybrid systems |b methods and protocols |c ed. by Paul N. MacDonald |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2001 | |
| 300 | |a XII, 336 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 650 | 4 | |a Protéines - Fixation - Recherche - Méthodologie | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a Plasmids | |
| 650 | 4 | |a Protein binding |x Research |x Methodology | |
| 650 | 4 | |a Two-Hybrid System Techniques | |
| 650 | 4 | |a Yeast fungi | |
| 650 | 4 | |a Yeasts |x genetics | |
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Record in the Search Index
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|---|---|
| adam_text | Contents
Preface v
Contributors xi
Part I General Methods
1 The Two Hybrid System: A Personal View
Stanley Fields and Paul L. Bartel 3
2 Growth and Maintenance of Yeast
Lawrence W. Bergman 9
3 Media Formulations for Various Two Hybrid Systems
Michael Saghbini, Denise Hoekstra, and Jim Gautsch 15
4 Yeast Two Hybrid Vectors and Strains
Philip James.... 41
5 High Efficiency Transformation of Plasmid DNA into Yeast
Robin A. Woods and R. Daniel Gietz 85
6 Qualitative and Quantitative Assessment of Interactions
Monica M. Montano 99
7 Strategies for Rescuing Plasmid DNA
from Yeast Two Hybrid Colonies
Alyson Byrd and Rene St Arnaud 707
Part II False Positives
8 Two Hybrid System and False Positives:
Approaches to Detection and Elimination
llya G. Serebriiskii and Erica A. Golemis 123
9 Confirming Yeast Two Hybrid Protein Interactions
Using In Vitro Glutathione S Transferase Pulldowns
Dennis M. Kraichely and Paul N. MacDonald 135
10 Two Hybrid Interactions Confirmed
by Coimmunoprecipitation of Epitope Tagged Clones
Louie Naumovski — — 151
ix
x Contents
Part ill Applications
11 Smad Interactors in Bone Morphogenetic Protein Signaling
Xiangli Yang and Xu Cao 163
12 Protein Interactions Important in Eukaryotic
Translation Initiation
Katsura Asano and Alan G. Hinnebusch 179
13 Steroid Receptor and Ligand Dependent Interaction
with Coactivator Proteins
Sergio A. Oriate 199
14 Interaction of Cellular Apoptosis Regulating Proteins
with Adenovirus Anti Apoptosis Protein E1B 19K
Thirugnana Subramanian and G. Chinnadurai 211
Part IV Alternative Strategies
15 Mammalian Two Hybrid Assays: Analyzing Protein Protein
Interactions in the Transforming Growth Factor fi
Signaling Pathway
Xin Hua Feng and Rik Derynck 221
16 One Hybrid Systems for Detecting Protein DNA Interactions
Mary Kate Alexander, Brenda D. Bourns,
and Virginia A. Zakian 241
17 The Split Hybrid System: Uncoding Multiprotein Networks
and Defining Mutations That Affect Protein Interactions
Phyllis S. Goldman, Anthony J. DeMaggio,
Richard H. Goodman, and Merl F. Hoekstra 261
18 Three Hybrid Screens: Inducible Third Party Systems
Bjorn Sandrock, Franck Tirode, and Jean Marc Egly 271
19 Three Hybrid Screens for RNA Binding Proteins:
Proteins Binding 3 End of Histone mRNA
Zbigniew Dominski and William F. Marzluff. 291
20 Membrane Recruitment Systems for Analysis
of Protein Protein Interactions
Ami Aronheim ,„. , 319
Index 329
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| id | DE-604.BV014047386 |
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| indexdate | 2024-07-09T18:56:42Z |
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| isbn | 0896038327 |
| language | English |
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| physical | XII, 336 S. Ill., graph. Darst. |
| publishDate | 2001 |
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| series | Methods in molecular biology |
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| spelling | Two-hybrid systems methods and protocols ed. by Paul N. MacDonald Totowa, NJ Humana Press 2001 XII, 336 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 177 Eiwitten gtt Gist gtt Levures (Botanique) Plasmiden gtt Plasmides Protéines - Fixation - Recherche - Méthodologie Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Plasmids Protein binding Research Methodology Two-Hybrid System Techniques Yeast fungi Yeasts genetics Rekombinantes Protein (DE-588)4277353-2 gnd rswk-swf Wechselwirkung (DE-588)4064937-4 gnd rswk-swf Yeast-Two-Hybrid-System (DE-588)4554290-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Rekombinantes Protein (DE-588)4277353-2 s Wechselwirkung (DE-588)4064937-4 s DE-604 Yeast-Two-Hybrid-System (DE-588)4554290-9 s Macdonald, Paul N. Sonstige oth Methods in molecular biology 177 (DE-604)BV035362695 177 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009619896&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Two-hybrid systems methods and protocols Methods in molecular biology Eiwitten gtt Gist gtt Levures (Botanique) Plasmiden gtt Plasmides Protéines - Fixation - Recherche - Méthodologie Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Plasmids Protein binding Research Methodology Two-Hybrid System Techniques Yeast fungi Yeasts genetics Rekombinantes Protein (DE-588)4277353-2 gnd Wechselwirkung (DE-588)4064937-4 gnd Yeast-Two-Hybrid-System (DE-588)4554290-9 gnd |
| subject_GND | (DE-588)4277353-2 (DE-588)4064937-4 (DE-588)4554290-9 (DE-588)4143413-4 |
| title | Two-hybrid systems methods and protocols |
| title_auth | Two-hybrid systems methods and protocols |
| title_exact_search | Two-hybrid systems methods and protocols |
| title_full | Two-hybrid systems methods and protocols ed. by Paul N. MacDonald |
| title_fullStr | Two-hybrid systems methods and protocols ed. by Paul N. MacDonald |
| title_full_unstemmed | Two-hybrid systems methods and protocols ed. by Paul N. MacDonald |
| title_short | Two-hybrid systems |
| title_sort | two hybrid systems methods and protocols |
| title_sub | methods and protocols |
| topic | Eiwitten gtt Gist gtt Levures (Botanique) Plasmiden gtt Plasmides Protéines - Fixation - Recherche - Méthodologie Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Plasmids Protein binding Research Methodology Two-Hybrid System Techniques Yeast fungi Yeasts genetics Rekombinantes Protein (DE-588)4277353-2 gnd Wechselwirkung (DE-588)4064937-4 gnd Yeast-Two-Hybrid-System (DE-588)4554290-9 gnd |
| topic_facet | Eiwitten Gist Levures (Botanique) Plasmiden Plasmides Protéines - Fixation - Recherche - Méthodologie Wetenschappelijke technieken Wissenschaftliches Arbeiten Plasmids Protein binding Research Methodology Two-Hybrid System Techniques Yeast fungi Yeasts genetics Rekombinantes Protein Wechselwirkung Yeast-Two-Hybrid-System Aufsatzsammlung |
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