Steroid receptor methods: protocols and assays
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2001
|
| Series: | Methods in molecular biology
176 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XIV, 381 S. graph. Darst. |
| ISBN: | 0896037541 |
Staff View
MARC
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| 245 | 1 | 0 | |a Steroid receptor methods |b protocols and assays |c ed. by Benjamin A. Lieberman |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2001 | |
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Record in the Search Index
| _version_ | 1804128853222752256 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
Part I. Bioinformatics 1
1 Bioinformatics of Nuclear Receptors
Mark Danielsen 3
2 Phylogenetic Inference and Parsimony Analysis
Llewellyn D. Densmore III 23
Part II. Purification Protocols 37
3 Expression and Purification of Recombinant Human Progesterone
Receptor in Baculovirus and Bacterial Systems
Vida Senkus Melvin and Dean P. Edwards 39
4 High Yield Purification of Functionally Active Glucocorticoid Receptor
Terace M. Fletcher, Barbour S. Warren, Christopher T. Baumann,
and Gordon L. Hager 55
5 Production and Purification of Histidine Tagged Dihydrotestosterone
Bound Full Length Human Androgen Receptor
Mingmin Liao and Elizabeth M. Wilson 67
6 Large Scale Production of Nuclear Receptor Ligand Binding Domains
Li Zhi Mi and Fraydoon Rastinejad 81
7 Physical Structure of Nuclear Receptor DNA Complexes
Scott A. Chasse and Fraydoon Rastinejad 91
8 Isolation of Steroid Regulated Genes from the Uterus by mRNA
Differential Display
Sushma Kumar, Maarit Angervo, Milan K. Bagchi,
and Indrani C. Bagchi 105
9 Identification of Nuclear Hormone Receptor Homologs by Screening
Libraries with Highly Degenerate Oligonucleotide Probes
Bruce Blumberg 119
Part III. Steroid Hormone Binding Assays 131
10 Use of [ Tc] Technetium Labeled Steroids as Probes for
Steroid Hormone Receptors
Frank Wust 133
vii
Vjli Contents
11 Steroid Hormone Metabolites and Hormone Binding Assays
Rosemary Bland and Martin Hewison 145
12 Vitamin D3 Analog Screening
Sami Vaisanen, Sanna Ryhanen, and Pekka H. Maenpaa 163
Part IV. Protein Interaction Assays 177
13 Application of Green Fluorescent Protein to the Study
of Dynamic Protein Protein Interactions and Subcellular
Trafficking of Steroid Receptors
Steven K. Nordeen, Paul R. Housley, Ylhong Wan,
and Richard N. Day 179
14 Knockout Mice and Steroid Receptor Research
PerFlodby, Stephen Teglund, andJan Ake Gustafsson 201
15 Yeast Two Hybrid Screening for Proteins that Interact with Nuclear
Hormone Receptors
Bertrand Le Douarin, David M. Heery, Claudine Gaudon,
Elmar vom Baur, and Regine Losson 227
16 Isolation of a p300/CBP Cointegrator Associated Protein
Coactivator Complex
Rabindra N. Bhattacharjee, Caroline Underhill,
and Joseph Torchia 249
17 Nonradioactive Photoaffinity Labeling of Steroid Receptors
Using Western Blot Detection System
Simon J. Evans and Frank L. Moore 261
18 Analysis of Steroid Hormone Induced Histone Acetylation
by Chromatin Immunoprecipitation Assay
James R. Lambert and Steven K. Nordeen 273
19 Analyzing the Contributions of Chromatin Structure
in Nuclear Hormone Receptor Activated Transcription In Vivo
Christy J. Fryer and Trevor K. Archer 283
20 Cotransfection Assays and Steroid Receptor Biology
Shimin Zhang and Mark Danielsen 297
21 Estrogen Receptor mRNA In Situ Hybridization Using Microprobe
System
Hironobu Sasano, Sachiko Matsuzaki, and Takashi Suzuki 317
Part V. Cancer Research and Drug Discovery 327
22 Solid Tumor Cancer Markers and Applications to Steroid Hormone
Research
Marcia V. Fournier, /Catherine J. Martin, and Arthur B. Pardee 329
Contents ix
23 Assessing Modulation of Estrogenic Activity of Environmental
and Pharmaceutical Compounds Using MCF 7 Focus Assay
Kathleen F. Arcaro and John F. Gierthy 341
24 Combinatorial Chemistry in Steroid Receptor Drug Discovery
John A. Flygare, Daniel P. Sutherlin, and S. David Brown 353
25 Identification of Nuclear Receptor Interacting Proteins Using Yeast
Two Hybrid Technology: Applications to Drug Discovery
Sunil Nagpgl, Corine R. Ghosn,
and Roshantha A.S. Chandraratna 359
Index 377
|
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| dewey-full | 612.4/05 |
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| dewey-raw | 612.4/05 |
| dewey-search | 612.4/05 |
| dewey-sort | 3612.4 15 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Medizin |
| format | Book |
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| indexdate | 2024-07-09T18:55:50Z |
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| isbn | 0896037541 |
| language | English |
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| physical | XIV, 381 S. graph. Darst. |
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| series | Methods in molecular biology |
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| spelling | Steroid receptor methods protocols and assays ed. by Benjamin A. Lieberman Totowa, NJ Humana Press 2001 XIV, 381 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 176 Hormoonreceptoren gtt Steroïdhormonen gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Receptors, Steroid Laboratory Manuals Steroid hormones Receptors Laboratory manuals Steroids Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Steroidhormonrezeptor (DE-588)4057381-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Steroidhormonrezeptor (DE-588)4057381-3 s Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s DE-604 Lieberman, Benjamin A. Sonstige oth Methods in molecular biology 176 (DE-604)BV035362695 176 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009582954&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Steroid receptor methods protocols and assays Methods in molecular biology Hormoonreceptoren gtt Steroïdhormonen gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Receptors, Steroid Laboratory Manuals Steroid hormones Receptors Laboratory manuals Steroids Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd Steroidhormonrezeptor (DE-588)4057381-3 gnd |
| subject_GND | (DE-588)4039983-7 (DE-588)4038971-6 (DE-588)4057381-3 (DE-588)4143413-4 |
| title | Steroid receptor methods protocols and assays |
| title_auth | Steroid receptor methods protocols and assays |
| title_exact_search | Steroid receptor methods protocols and assays |
| title_full | Steroid receptor methods protocols and assays ed. by Benjamin A. Lieberman |
| title_fullStr | Steroid receptor methods protocols and assays ed. by Benjamin A. Lieberman |
| title_full_unstemmed | Steroid receptor methods protocols and assays ed. by Benjamin A. Lieberman |
| title_short | Steroid receptor methods |
| title_sort | steroid receptor methods protocols and assays |
| title_sub | protocols and assays |
| topic | Hormoonreceptoren gtt Steroïdhormonen gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Receptors, Steroid Laboratory Manuals Steroid hormones Receptors Laboratory manuals Steroids Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd Steroidhormonrezeptor (DE-588)4057381-3 gnd |
| topic_facet | Hormoonreceptoren Steroïdhormonen Wetenschappelijke technieken Wissenschaftliches Arbeiten Receptors, Steroid Laboratory Manuals Steroid hormones Receptors Laboratory manuals Steroids Laboratory Manuals Molekularbiologie Methode Steroidhormonrezeptor Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009582954&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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