The role of protein kinase C d in B cell immunity:
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| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
2001
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Köln, Univ., Diss., 2001 |
| Physical Description: | 110 S. Ill., graph. Darst. : 21 cm |
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MARC
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| 003 | DE-604 | ||
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| 008 | 011023s2001 gw ad|| m||| 00||| eng d | ||
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| 035 | |a (OCoLC)635034911 | ||
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| 044 | |a gw |c DE | ||
| 049 | |a DE-355 | ||
| 100 | 1 | |a Mecklenbräuker, Ingrid |d 1971- |e Verfasser |0 (DE-588)12321968X |4 aut | |
| 245 | 1 | 0 | |a The role of protein kinase C d in B cell immunity |c vorgelegt von Ingrid Mecklenbräuker |
| 246 | 1 | 3 | |a The role of protein kinase C delta in B cell immunity |
| 264 | 1 | |c 2001 | |
| 300 | |a 110 S. |b Ill., graph. Darst. : 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Köln, Univ., Diss., 2001 | ||
| 650 | 0 | 7 | |a Zelluläre Immunität |0 (DE-588)4197656-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Proteinkinase C |0 (DE-588)4306407-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a B-Lymphozyt |0 (DE-588)4137121-5 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a B-Lymphozyt |0 (DE-588)4137121-5 |D s |
| 689 | 0 | 1 | |a Zelluläre Immunität |0 (DE-588)4197656-3 |D s |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009572499 | |
Record in the Search Index
| _version_ | 1807322672756948992 |
|---|---|
| adam_text |
TABLE
OF
CONTENTS
A.
INTRODUCTION
.
1
A.1.
HISTORY
OF
THE
PKCS
.
1
A.2.
THE
PKC
GENE
FAMILY
.
2
A.3.
STRUCTURAL
AND
FUNCTIONAL
DOMAINS
IN
PKC
PROTEINS
.
4
A.4.
THE
EXPRESSION
OF
PKCS
IN
HEMATOPOIETIC
CELLS
.
7
A.4.
1.
PKC
ISOFORMS
IN
T
CELLS
.
8
A.4.2.
PKC
ISOFORMS
IN
B
CELLS
.
10
A.5.
PKC
BINDING
PROTEINS
.
12
A.6.
ACTIVATION
AND
TRANSLOCATION
OF
PKC
.
13
A.7.
STIMULATION
OF
PKC
ACTIVITY
.
13
A.8.
B
CELL
ANTIGEN
RECEPTOR-MEDIATED
SIGNAL
TRANSDUCTION
.
15
A.8.1.
PKCS
IN
B
LYMPHOCYTES
.
17
A.9.
BIOLOGICAL
FUNCTIONS
OF
PKC8
.
17
A.
10.
GENERATION
OF
PKCS
A
MICE
.
19
A.11.
THE
AIMS
OF
THIS
STUDY
.
21
B.
MATERIALS
AND
METHODS
.
22
B.
1.
MOLECULAR
BIOLOGY
.
22
B.
1.1.
DNA
MANIPULATION
.
22
B.1
.2.
MIDI
AND
MINI
PREPARATION
OF
PLASMID
DNA
.
22
B.1
.3.
DNA
MANIPULATION
AND
AGAROSE
GEL
ELECTROPHORESIS
.
22
B.1
.4.
RECOVERING
OF
DNA
FROM
AGAROSE
GELS
.
22
B.1
.5.
ISOLATION
OF
GENOMIC
DNA
FROM
MOUSE
TAIL
TIPS
.
22
B.1
.6.
SOUTHERN
BLOT
ANALYSIS
.
23
B.
1.7.
DNA
AMPLIFICATION
BY
POLYMERASE
CHAIN
REACTION
(PCR)
.
23
B.2.
CELLULAR
METHODS
.
24
B.2.
1.
PREPARATION
OF
MURINE
LYMPHOCYTE
SUSPENSIONS
.
24
B.2.2.
MAGNETIC
CELL
SORTING
(MACS)
.
25
B.2.3.
CELL
STAINING
AND
FLOW
CYTOMETRY
.
25
B.2.4.
CELL
SURVIVAL
ASSAY
.
26
B.2.5.
ANNEXIN
V
STAINING
PROCEDURE
.
26
B.2.6.
PREPARATION
OF
MEDIUM
FOR
IN
VITRO
CELL
CULTURE
.
26
B.2.7.
SPLENIC
B
CELL
IN
VITRO
CULTURE
.
27
B.2.8.
ANALYSIS
OF
B
CELL
PROLIFERATION
AND
EXPRESSION
OF
B
CELL
ACTIVATION
MARKERS
.
27
B.2.9.
ANALYSIS
OF
CELL
DIVISION
(CFSE
STAINING)
.
27
B.2.10.
CALCIUM
FLUX
ANALYSIS
.
28
B.2.1
1.
FLUORESCENCE-ACTIVATED
CELL
SORTER
(FACS)-P-GAL
ANALYSIS
.
28
B.2.1
2.
ELISA
AND
ELISPOT
.
29
B.2.12.1.
SERUM
ANTI-LYSOZYME
K
ELISA
.
29
B.2.1
2.2.
SERUM
LYSOZYME
(HEL)
TITERS
.
29
B.2.1
3.
IN
VIVO
EXPERIMENTS
.
30
B.3.
MISCELLANEOUS
.
30
B.4.
MICE
.
31
C.
RESULTS
.
32
C.1
.
EXPRESSION
OF
THE
PKCD-LACZ
FUSION
ALLELE
IN
LYMPHOCYTES
.
32
C.2.
LYMPHOCYTE
SUBPOPULATIONS
IN
PKC8
+
MICE
.
35
C.2.
1.
PERIPHERAL
LYMPHOCYTE
NUMBERS
IN
PKC8
+
MICE
.
39
C.3.
SERUM
IMMUNOGLOBULIN
LEVELS
IN
NON-IMMUNIZED
PKC8
'
/_
MICE
.
42
C.3.1
.
ANTIBODY
SECRETING
CELLS
IN
THE
BONE
MARROW
AND
SPLEEN
OF
PKC5
_/
'
MICE
.
42
C.4.
HUMORAL
IMMUNE
RESPONSES
IN
PKC8'
/_
MICE
.
45
C.4.1
.
CROSS
REACTIVE
ANTIBODIES
IN
THE
SERUM
OF
PKC8
/_
MICE
.
47
C.5.
B
CELL
ACTIVATION
IN
VITRO.
UPREGULATION
OF
ACTIVATION
MARKERS
.
47
C.5.1
.
B
CELL
PROLIFERATION
IN
VITRO
.
47
C.5.2.
CA
2+
MOBILIZATION
IN
B
CELLS
.
50
C.6.
CELL
SURVIVAL
IN
VITRO
.
50
C.6.1
.
SPONTANEOUS
AND
INDUCED
APOPTOSIS
IN
VITRO
.
53
C.6.2.
CD95/FAS
MEDIATED
APOPTOSIS
IN
VITRO
.
53
C.7.
MAINTENANCE
OF
SELF-TOLERANCE
.
54
C.7.1.
BREEDING
OF
IGHEL
AND
SHEL
TRANSGENES
ONTO
THE
PKC8
A
.
BACKGROUND
.
55
C.7.2.
LYMPHOCYTE
SUBPOPULATIONS
IN
IGHEL
PKC8
/_
MICE
.
55
C.7.3.
CLONAL
DELETION
OF
PKC8
'
_
IGHEL
B
LYMPHOCYTES
BY
MEMBRANE-BOUND
HEL
.
58
C.7.4.
DEFECTIVE
FUNCTIONAL
INACTIVATION
OF
PKC8
/
IGHEL
B
LYMPHOCYTES
IN
THE
PRESENCE
OF
SELF-ANTIGEN
.
58
C.7.5.
AUTOANTIBODIES
IN
IGHEL;
SHEL
TRANSGENIC
PKC8'
/_
MICE
.
63
C.7.6.
LEVELS
OF
SELF-ANTIGEN
IN
THE
SERUM
OF
PKC8'
/_
IGHEL;
SHEL
MICE
.
65
C.7.7.
RESPONSE
TO
ANTIGEN
EX
VIVO
AND
EXPRESSION
OF
ACTIVATION
MARKERS
.
67
C.7.8.
CA
2+
MOBILIZATION
IN
PKC8
/_
B
CELLS
FROM
IGHEL;
SHEL
MICE
.
70
C.7.9.
POLYCLONAL
ACTIVATION
OF
PKC8
-/
'
B
CELLS
FROM
IGHEL;
SHEL
MICE
IN
VITRO
.
70
D.
DISCUSSION
.
73
D.1
.
ESSENTIAL
ROLE
FOR
PKC8
IN
SELF
ANTIGEN
INDUCED
B
CELL
ANERGY
.
73
D.2.
THE
PKCD-LACZ
FUSION
ALLELE
IS
HIGHLY
EXPRESSED
IN
B
LINEAGE
CELLS
OF
PKC8
+/
'
MICE
.
74
D.3.
PKC8
IS
NOT
REQUIRED
FOR
NORMAL
LYMPHOCYTE
DEVELOPMENT
.
76
D.4.
A
ROLE
FOR
PKC8
IN
THE
REGULATION
OF
THE
PERIPHERAL
LYMPHOCYTE
POOL?
77
D.5.
DOES
DEFICIENCY
IN
PKC8
AFFECT
SURVIVAL
OF
B
CELLS
IN
VIVO?
.
78
D.6.
PKC8-DEFICIENT
MICE
HAVE
INCREASED
LEVELS
OF
SERUM
IMMUNOGLOBULIN
IGM,
LGG1
AND
IGA
ISOTYPES
.
78
D.7.
PKC8-DEFICIENT
MICE
RESPOND
EFFICIENTLY
TO
T
CELL-DEPENDENT
AND
-INDEPENDENT
ANTIGENS
.
79
D.8.
THE
SERUM
OF
PKC8
_/
'
MICE
CONTAINS
HIGH
LEVELS
OF
POLYREACTIVE
ANTIBODIES
.
80
D.9.
PKC8
IS
NOT
REQUIRED
FOR
B
CELL
ACTIVATION
IN
VITRO
.
80
D.10.
NORMAL
SUSCEPTIBILITY
OF
PKC3
'
A
CELLS
TO
VARIOUS
APOPTOTIC
STIMULI
.
81
D.1
1.
MAINTENANCE
OF
SELF-TOLERANCE
.
83
D.12.
CLONAL
DELETION
OF
PKCS
'
A
IGHEL
B
LYMPHOCYTES
BY
MEMBRANE
BOUND
HEL
.
83
D.13.
IN
CONTRAST
TO
WILD-TYPE
MICE,
PKCS^
HEL-BINDING
B
CELLS
DEVELOP
NORMALLY
AND
ACCUMULATE
IN
THE
PRESENCE
OF
SHEL
SELF-ANTIGEN
.
84
D.14.
SPONTANEOUS
PRODUCTION
OF
ANTI-HEL
ANTIBODIES
IN
PKC8
_/
'
IGHEL;
SHEL
MICE
.
85
D.15.
PKC8-DEFICIENT
IGHEL;
SHEL
B
CELLS
ARE
HYPERRESPONSIVE
IN
RESPONSE
TO
BCR
CROSS-LINKING
.
87
D.16.
PKC8
IS
INVOLVED
IN
POLYCLONAL
ACTIVATION
OF
B
CELLS
IN
VITRO
.
87
D.17.
WHAT
IS
THE
ROLE
OF
PKC8
IN
T
CELL
TOLERANCE?
.
88
D.18.
ALTERATION
IN
BCR
SIGNALING
AFFECTS
ALSO
TOLERANCE
IN
SHEL;
IGHEL
DOUBLE
TRANSGENIC
MICE
.
89
E.
ABSTRACT
.
91
F.
KURZE
ZUSAMMENFASSUNG
.
91
G.
SUMMARY
.
92
H.
ZUSAMMENFASSUNG
.
93
I.
REFERENCES
.94
J.
ACKNOWLEDGEMENTS
.
110
K.
LEBENSLAUF
.
111
L.
ERKLARUNG
.
112
M.
PUBLICATIONS
.
113 |
| any_adam_object | 1 |
| author | Mecklenbräuker, Ingrid 1971- |
| author_GND | (DE-588)12321968X |
| author_facet | Mecklenbräuker, Ingrid 1971- |
| author_role | aut |
| author_sort | Mecklenbräuker, Ingrid 1971- |
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| building | Verbundindex |
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| ctrlnum | (OCoLC)635034911 (DE-599)BVBBV013984318 |
| format | Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV013984318 |
| illustrated | Illustrated |
| indexdate | 2024-08-14T01:00:12Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009572499 |
| oclc_num | 635034911 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 110 S. Ill., graph. Darst. : 21 cm |
| publishDate | 2001 |
| publishDateSearch | 2001 |
| publishDateSort | 2001 |
| record_format | marc |
| spelling | Mecklenbräuker, Ingrid 1971- Verfasser (DE-588)12321968X aut The role of protein kinase C d in B cell immunity vorgelegt von Ingrid Mecklenbräuker The role of protein kinase C delta in B cell immunity 2001 110 S. Ill., graph. Darst. : 21 cm txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 2001 Zelluläre Immunität (DE-588)4197656-3 gnd rswk-swf Proteinkinase C (DE-588)4306407-3 gnd rswk-swf B-Lymphozyt (DE-588)4137121-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content B-Lymphozyt (DE-588)4137121-5 s Zelluläre Immunität (DE-588)4197656-3 s Proteinkinase C (DE-588)4306407-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009572499&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mecklenbräuker, Ingrid 1971- The role of protein kinase C d in B cell immunity Zelluläre Immunität (DE-588)4197656-3 gnd Proteinkinase C (DE-588)4306407-3 gnd B-Lymphozyt (DE-588)4137121-5 gnd |
| subject_GND | (DE-588)4197656-3 (DE-588)4306407-3 (DE-588)4137121-5 (DE-588)4113937-9 |
| title | The role of protein kinase C d in B cell immunity |
| title_alt | The role of protein kinase C delta in B cell immunity |
| title_auth | The role of protein kinase C d in B cell immunity |
| title_exact_search | The role of protein kinase C d in B cell immunity |
| title_full | The role of protein kinase C d in B cell immunity vorgelegt von Ingrid Mecklenbräuker |
| title_fullStr | The role of protein kinase C d in B cell immunity vorgelegt von Ingrid Mecklenbräuker |
| title_full_unstemmed | The role of protein kinase C d in B cell immunity vorgelegt von Ingrid Mecklenbräuker |
| title_short | The role of protein kinase C d in B cell immunity |
| title_sort | the role of protein kinase c d in b cell immunity |
| topic | Zelluläre Immunität (DE-588)4197656-3 gnd Proteinkinase C (DE-588)4306407-3 gnd B-Lymphozyt (DE-588)4137121-5 gnd |
| topic_facet | Zelluläre Immunität Proteinkinase C B-Lymphozyt Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009572499&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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