Characterization of protein kinase C in signal transduction of thrombin induced human platelets:
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| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
2000
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Bochum, Univ., Diss., 2001 |
| Physical Description: | 67 Bl. graph. Darst. : 30 cm |
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CONTENT
1.
INTRODUCTION.7
1.1. DIABETES MELLITUS.7
1.1.2. COMPLICATIONS OF TYPE 2 DIBETES MELLITUS.11
1.1.3. THROMBOXANE BIOSYNTHESIS.12
1.1.4. PROTEIN KINASE C: STRUCTURE, FUNCTION, AND REGULATION.13
1.1.5. CELLULAR FUNCTIONS ON PKC ISOENZYMES AND THEIR ROLE IN DISEASE.17
1.1.6. SIGNAL TRANSDUCTION IN THROMBIN- STIMULATED PLATELETS.18
1.1.7. THE PURPOSE OF THIS STUDY.19
2. SUBJECTS, MATERIALS AND METHODS
.20
2.1. SUBJECTS. 20
2.2. PREPARATION AND INCUBATION OF HUMAN PLATELETS.
MEASUREMENT OF THROMBOXANE B2 LEVELS.21
2.2. 1. METHOD.21
2.2.2. MATERIALS.22
2.3. PREPARATION OF CELL HOMOGENATES.22
2.3.1. METHOD.22
2.3.2. MATERIALS.23
2.4. QUANTITATION OF THE AMOUNT OF PROTEIN: STOSHEK- MODIFIED ON
BRADFORD ASSAY.23
2.4.1. METHOD.23
2.4.2. MATERIALS.'.24
2.5. SDS- POLYACRYLAMIDE GEL ELECTROFORESIS OF PROTEINS.24
2.5.1. METHOD.24
2. 5.2. MATERIALS.25
J
2.6. TRANSFER OF PROTEINS FROM SDS- POLYACRYLAMIDE GELS TO SOLID
SUPPORTS. 26
2.6.1. METHOD.26
2.6.2. MATERIALS.27
2.7. IMMUNOBLOTTING.28
2. 7. 1. METHOD.28
2. 7.2. MATERIALS.29
2.8. QUANTITATION OF PROTEIN KINASE SIGNALS BY DENSITOMETRIC TRACING OF
THE MEMBRANE. 30
2. 8.1. MATERIALS.30
2.8.2. METHOD.30
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/962684651
2. 8. 3. DATA ANALYSIS.31
3. 1. RESULTS
.32
3. 3.1. THROMBIN- INDUCED TXB2 RELEASE FROM PLATELETS OF T2DM PATIENTS
AND CONTROL.32
3. 3. 2. EFFECTS OF PKC INHIBITORS ON THROMBIN INDUCED- TXB2 LEVELS.34
3. 3. 3. EFFECTS OF THROMBIN ON TRANSLOCATION ON PKC ISOFORMS.36
3. 3. 4. EFFECTS OF PKCA/ P OR PKC PSEUDOSUBSTRATE INHIBITOR PEPTIDE.41
4. DISCUSSION
.43
5. SUMMARY
.50
6. REFERENCES
.51
CURRICULUM VITAE.68
*
8 |
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| indexdate | 2024-10-09T18:08:41Z |
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| physical | 67 Bl. graph. Darst. : 30 cm |
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| spelling | Bumbure, Alda 1967- Verfasser (DE-588)123198577 aut Characterization of protein kinase C in signal transduction of thrombin induced human platelets presented by Alda Bumbure 2000 67 Bl. graph. Darst. : 30 cm txt rdacontent n rdamedia nc rdacarrier Bochum, Univ., Diss., 2001 Hochschulschrift (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009572378&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bumbure, Alda 1967- Characterization of protein kinase C in signal transduction of thrombin induced human platelets Hochschulschrift |
| subject_GND | (DE-588)4113937-9 |
| title | Characterization of protein kinase C in signal transduction of thrombin induced human platelets |
| title_auth | Characterization of protein kinase C in signal transduction of thrombin induced human platelets |
| title_exact_search | Characterization of protein kinase C in signal transduction of thrombin induced human platelets |
| title_full | Characterization of protein kinase C in signal transduction of thrombin induced human platelets presented by Alda Bumbure |
| title_fullStr | Characterization of protein kinase C in signal transduction of thrombin induced human platelets presented by Alda Bumbure |
| title_full_unstemmed | Characterization of protein kinase C in signal transduction of thrombin induced human platelets presented by Alda Bumbure |
| title_short | Characterization of protein kinase C in signal transduction of thrombin induced human platelets |
| title_sort | characterization of protein kinase c in signal transduction of thrombin induced human platelets |
| topic | Hochschulschrift |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009572378&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT bumburealda characterizationofproteinkinasecinsignaltransductionofthrombininducedhumanplatelets |