Studies on the diversity of purple sulfur bacteria in the Baltic Sea, Lake Nasser (Egypt) and the Red Sea based on classical and molecular genetic approaches:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2001
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Kiel, Univ., Diss., 2001 |
| Beschreibung: | III, 136 Bl. Ill., graph. Darst., Kt. : 30 cm |
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Datensatz im Suchindex
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| adam_text |
CONTENTS
_I
CONTENTS
SUBJECT PAGE
1. INTRODUCTION AND AIM OF WORK.1
2. MATERIALS AND METHODS_._.13
2.1 COLLECTION OF SAMPLES.13
2.1.1 COLLECTION OF SAMPLES OF THE BALTIC SEA.13
2.1.2 COLLECTION OF SAMPLES OF LAKE NASSER (EGYPT) AND THE RED SEA
SHORE.18
2.2 ISOLATION OF CHROMATIACEAE. 21
2.2.1 MEDIA. 21
2.2.2 ISOLATION OF PURE CULTURES.25
2.2.3 TOTAL COUNTS.27
2.3 MOLECULAR GENETIC APPROACHES.28
2.3.1 DNA EXTRACTION FROM SEDIMENTS.28
2.3.2 EXTRACTION OF CHROMOSOMAL DNA FROM ENRICHMENT CULTURES AND
ISOLATES .29
2.3.3 AGAROSE GEL ELECTROPHORESIS.29
2.3.4 POLYMERASE CHAIN REACTION (PCR).30
2.3.4.1 DNA-AMPLIFICATION USING "READY -TO- GO" PCR BEADS.31
2.3.4.2 PCR PRIMERS TO AMPLIFY 16S RDNA FROM CHROMATIACEAE.32
2.3.5 DENATURING GRADIENT GEL ELECTROPHORESIS (DGGE).34
2.3.5.1 ASSEMBLING THE PARALLEL GRADIENT GEL SANDWICH.36
2.3.5.2 STAINING OF DGGE-GELS.38
2.3.5.3 EXTRACTION OF DNA FROM DGGE GELS.39
2.3.5.4 PCR OF THE DGGE BANDS.39
2.3.6 PURIFICATION OF PCR AMPLICONS FOR SEQUENCING.41
2.3.7 DNA SEQUENCING OF THE AMPLIFIED 16S RDNA .42
2.3.8 SEQUENCING REACTION WITH PCR PRODUCTS.42
2.3.9 IDENTIFICATION AND CONSTRUCTION OF PHYLOGENETIC TREES.43
3. EXPERIMENTAL RESULTS. 45
3.1 TOTAL COUNTS OF PURPLE SULFUR BACTERIA IN SEDIMENT SAMPLES OF LAKE
NASSER.45
3.2 SPECIFIC AMPLIFICATION OF 16S RDNA FROM CHROMATIACEAE.47
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/961909358
CONTENTS
II
3.2.1 AMPLIFICATION FROM REFERENCE STRAINS.47
3.2.2 AMPLIFICATION FROM SEDIMENT SAMPLES.50
3.2.3 AMPLIFICATION FROM ENRICHMENT CULTURES.53
3.3 DENATURING GRADIENT GEL ELECTROPHORESIS (DGGE).56
3.3.1 DGGE PROFILES FROM THE BALTIC SEA SEDIMENTS.56
3.3.2 DGGE PROFILES FROM THE RED SEA & LAKE NASSER SEDIMENT SAMPLES.59
*
3.3.3 COMPARISON OF DGGE BAND PATTERNS FROM SEDIMENT SAMPLES AND
ENRICHMENT
CULTURES.63
3.3.3.1 BALTIC SEA LOCATIONS.63
3.3.3.2 LAKE NASSER LOCATIONS.69
3.3.3.3 RED SEA LOCATIONS.73
3.3.4 COMPARISON OF DGGE BAND PATTERNS OF ENRICHMENT CULTURES FROM THE
RED SEA
AND LAKE NASSER.74
3.4 IDENTIFICATION OF PURE CULTURE ISOLATES BY COMPARATIVE SEQUENCE
ANALYSIS.75
3.5 PHYLOGENETIC POSITION OF SEQUENCES FROM DGGE BANDS AND ISOLATES.76
4. DISCUSSION.79
4.1 TOTAL COUNTS OF PURPLE SULFUR BACTERIA IN LAKE NASSER.81
4.2 AMPLIFICATION OF 16S RDNA FROM MIXED BACTERIAL CULTURES AND
ENVIRONMENTAL SAMPLES.82
4.3 ANALYSIS OF BACTERIAL COMMUPITIES BY DGGE.83
4.4 PHYLOGENETIC POSITION AND IDENTIFICATION OF DGGE BANDS AND
ISOLATES.86
4.5 PROSPECTIVES FOR FUTURE WORK.88
5. SUMMARY. 90
6. ZUSAMMENFASSUNG. 92
7. REFERENCES. 94
8. APPENDIX
114 |
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| language | English |
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| physical | III, 136 Bl. Ill., graph. Darst., Kt. : 30 cm |
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| spelling | Younis, Magdi Verfasser aut Studies on the diversity of purple sulfur bacteria in the Baltic Sea, Lake Nasser (Egypt) and the Red Sea based on classical and molecular genetic approaches presented by Magdi Younis 2001 III, 136 Bl. Ill., graph. Darst., Kt. : 30 cm txt rdacontent n rdamedia nc rdacarrier Kiel, Univ., Diss., 2001 Schwefelpurpurbakterien (DE-588)4180423-5 gnd rswk-swf Artenreichtum (DE-588)4131912-6 gnd rswk-swf Nassersee (DE-588)4041268-4 gnd rswk-swf Ostsee (DE-588)4044107-6 gnd rswk-swf Rotes Meer (DE-588)4050664-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ostsee (DE-588)4044107-6 g Schwefelpurpurbakterien (DE-588)4180423-5 s Artenreichtum (DE-588)4131912-6 s DE-604 Nassersee (DE-588)4041268-4 g Rotes Meer (DE-588)4050664-2 g DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009476815&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Younis, Magdi Studies on the diversity of purple sulfur bacteria in the Baltic Sea, Lake Nasser (Egypt) and the Red Sea based on classical and molecular genetic approaches Schwefelpurpurbakterien (DE-588)4180423-5 gnd Artenreichtum (DE-588)4131912-6 gnd |
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| title | Studies on the diversity of purple sulfur bacteria in the Baltic Sea, Lake Nasser (Egypt) and the Red Sea based on classical and molecular genetic approaches |
| title_auth | Studies on the diversity of purple sulfur bacteria in the Baltic Sea, Lake Nasser (Egypt) and the Red Sea based on classical and molecular genetic approaches |
| title_exact_search | Studies on the diversity of purple sulfur bacteria in the Baltic Sea, Lake Nasser (Egypt) and the Red Sea based on classical and molecular genetic approaches |
| title_full | Studies on the diversity of purple sulfur bacteria in the Baltic Sea, Lake Nasser (Egypt) and the Red Sea based on classical and molecular genetic approaches presented by Magdi Younis |
| title_fullStr | Studies on the diversity of purple sulfur bacteria in the Baltic Sea, Lake Nasser (Egypt) and the Red Sea based on classical and molecular genetic approaches presented by Magdi Younis |
| title_full_unstemmed | Studies on the diversity of purple sulfur bacteria in the Baltic Sea, Lake Nasser (Egypt) and the Red Sea based on classical and molecular genetic approaches presented by Magdi Younis |
| title_short | Studies on the diversity of purple sulfur bacteria in the Baltic Sea, Lake Nasser (Egypt) and the Red Sea based on classical and molecular genetic approaches |
| title_sort | studies on the diversity of purple sulfur bacteria in the baltic sea lake nasser egypt and the red sea based on classical and molecular genetic approaches |
| topic | Schwefelpurpurbakterien (DE-588)4180423-5 gnd Artenreichtum (DE-588)4131912-6 gnd |
| topic_facet | Schwefelpurpurbakterien Artenreichtum Nassersee Ostsee Rotes Meer Hochschulschrift |
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