Experimental protocols for reactive oxygen and nitrogen species:
Saved in:
| Format: | Book |
|---|---|
| Language: | English Japanese |
| Published: |
Oxford u.a.
Oxford Univ. Press
2000
|
| Edition: | 1. publ. |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XIX, 337 S. Ill., graph. Darst. |
| ISBN: | 0198506686 |
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Record in the Search Index
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|---|---|
| adam_text | Experimental
protocols for
reactive oxygen and
nitrogen species
Edited by
NAOYUKI TANIGUCHI
Department of Biochemistry
Osaka University Medical School
and
JOHN M C GUTTERIDGE
Oxygen Chemistry Laboratory, Directorate of Anaesthesia and
Critical Care, Royal Brompton and Harefield NHS Trust
This publication was supported by a generous donation
from the Daido Life Foundation
OXPORD
UNIVERSITY PRESS
Contents
Con trib u ting auth ors w
PARTI: MEASUREMENT
1 Oxygen uptake, and spectrophotometric measurement of
superoxide, hydrogen peroxide and hydroxyl radicals
JMC Gutteridge and G J Quinlan 3
2 Measurements of nitric oxide and peroxynitrite
Kohji lchimori 7
3 Chemiluminescence
Minoru Nakano and Yasuhiro Kambayashi 13
4 ESR Spin trapping
Hiroaki Kosaka 17
5 Nitrosothiol detection by HPLC coupled with flow
reactors of Hg2+ and Griess reagent
Takaaki Akaike and Katsuhisa Inoue 20
6 NO-generating system
Junichi Fujii 25
7 Superoxide-generating system
Keiichiro Suzuki 28
8 ROS-generating systems in mitochondria, microsomes
and peroxisomes
Dongchon Kang and Koichiro Takeshige 30
9 Neutrophils and priming Isolation of neutrophils/assay
of O2-~ generation by cytochrome-c reduction
Toshihiko Utsumi and Kozo Utsumi 40
10 Bioassay for antioxidant activity: determination of
scavenging capacity of antioxidants against lipid peroxyl
radicals
Yoichi Miyamoto, Takaaki Akaike, and Hiroshi Maeda 45
11 Iron-binding and iron-oxidizing antioxidant activities
JMC Gutteridge and G J Quinlan 50
12 Glutathione (oxidized and reduced form): assay mixture
and measurement
Takahiko Kondo and Fumi Sawada 57
vii
Contents
13 Measurement of proteinaceous plasma antioxidants,
low molecular weight antioxidants, and redox related
compounds
Yuki Kitaoka and Junji Yodoi 61
14 Vitamin E
Hiroshi Tamai 65
15 Vitamin C
Tohru Ogihara 67
16 CoQ: Extraction of CoQ homologues from animal
tissues / HPLC
Tatsuya Matsura 69
17 Vitamin A and carotenoids
Hiroshi Tamai 72
18 Determination of flavonoids (catechins) by HPLC-ECD
Isao Tomita and Mitsuaki Sano 74
19 Catalase
Naohisa Kawamura 77
20 Glutathione peroxidase: coupled enzyme assay
KazuhikoTakahashi 79
21 Glutathione reductase
Kenji Aki 81
22 Glutathione transferase
Shigeki Tsuchida 83
23 •Y-Glutamyl transpeptidase
Yoshitaka Ikeda 86
24 -y- Glutamylcysteine synthetase: assay mixture and
measurement
Takahiko Kondo and Fumi Sawada 88
25 Measurement of Mn-SOD and Cu,Zn-SOD
Keiichiro Suzuki 91
26 EC-SOD activity assay/immunochemical assay
Tetsuo Adachi 96
27 Nitric oxide synthase (NOS) activity assay: RI method to
detect L-citrulline
JunichiFujii 101
viii
Contents
28 Nitric oxide synthase (NOS) activity assay:
determination of produced NADP+
Junichi Fujii 103
29 Myeloperoxidase
Masao Nakamura 106
30 Haem oxygenase
Tadashi Yoshida 108
31 Caeruloplasmin
Toshihiro Sugiyama, Yoshihiko Kawarada, and Fujiko Hirasawa 111
32 Caeruloplasmin ferroxidase activity
JMC Gutteridge and G J Quinlan 114
33 Ferritin
Junji Kato, Koshi Fujikawa, and Yoshiro Niitsu 116
34 Histochemical staining of ROS: NBT and O2-7H2O2 and
cerium methods
Kei-ichi Hirai 119
35 Immunostaining of Mn-SOD, Cu,Zn-SOD and NOS
Masahiro Nakamura, Haruyuki Tatsumi, and Hiroki Nogawa 126
36 Xanthine oxidase
Takaaki Akaike, Yoichi Miyamoto, and Tomohiro Sawa 136
37 Plasma free fatty acids
Yoshihiro Yamamoto, Akio Fujisawa, Yuichiro Nagata, and
Satoshi Yamashita 142
38 Phospholipids and their hydroperoxides
Yoshihiro Yamamoto, Yasuhiro Kambayashi, and Satoshi Yamashita 144
39 Cholesterol and its oxides
Teruo Miyazawa 147
40 LDL isolation and copper-catalysed oxidation
Masayuki Yokode and Toru Kita 149
41 Diene conjugation
Hiroyuki Shimasaki 152
42 Fluorescent products
Hiroyuki Shimasaki 154
43 Lipid peroxides
EtsuoNiki 156
ix
Contents
44 Flow-cytometric analysis of lipid peroxides using
DCFH-DA
Yoshiro Kayanoki 161
45 Lipid peroxidation models
Noriko Noguchi and Etsuo Niki 164
46 4-Hydroxy-2-nonenal in plasma
G J Quinlan and JMC Gutteridge 169
47 Total plasma iron
JMC Gutteridge and G J Quinlan 172
48 Total plasma non-haem iron
JMC Gutteridge and S Mumby 174
49 Plasma unsaturated iron-binding capacity and percent
saturation of transferrin
JMC Gutteridge and S Mumby 176
50 Plasma transferrin
JMC Gutteridge and S Mumby 178
51 Low molecular mass chelatable iron: Reactive iron
species (RIS)
JMC Gutteridge and S Mumby 179
52 Speciation of ferrous ions in biological fluids
JMC Gutteridge 182
53 Tissue low molecular mass iron
JMC Gutteridge 183
54 Total plasma copper
JMC Gutteridge and G J Quinlan 185
55 Low molecular mass chelatable copper: reactive copper
species (RCS)
JMC Gutteridge 186
PART 2: GENE ANALYSES
56 Confocal analysis for oxidized states in cells
KiyoshiNose 191
57 Detection of nitric oxide synthase (NOS) expression:
preparation of RNA
JunichiFujii 194
Contents
58 Mn-SOD cell stimulation by cytokines: preparation of RNA
Keiichiro Suzuki 197
59 The expression of thioredoxin
Yoshihisa Taniguchi 200
60 Peroxiredoxin
Shiro Bannai 203
61 Expression of bcl-2
Yutaka Eguchi and Yoshihide Tsujimoto 207
62 Glutathione peroxidase: preparation of haemolysate and
cell homogenates/fixation of tissue/RNA extraction
Tetsuya Moriuchi 214
63 Expression of catalase gene
KenzoSato 217
64 L-Gulono- Y-lactone oxidase
Morimitsu Nishikimi 220
65 8-OH-dG: extraction/enzyme treatment/measurement
of8-OH-dG
Shinya Asami and Hiroshi Kasai 224
66 Detection of DNA damage and analysis of its
site-specificity
Shinji Oikawa and Shosiike Kawanishi 229
67 Detection of DNA/protein cleavages by glycation
Hideaki Kaneto and Naoyuki Taniguchi 236
68 4-hydroxy-2-nonenal protein adducts
Koji Uchida 239
69 Assay for 8-oxodGTP hydrolase
Hisashi Maki 242
70 Mitochondrial DNA damage
Mika Hayakawa and Takayuki Ozawa 245
71 Hydroxyl radical damage to DNA bases detected by
GC-MS
G J Quinlan and JMC Gutteridge 253
72 Peroxynitrite damage to DNA
JMC Gutteridge 256
73 Carbonyl assay for oxidative damage to proteins
G J Quinlan and JMC Gutteridge 257
xi
Contents
74 ROS damage to protein
Koji Uchida 259
75 Damage to proteins by reactive oxygen and reactive
nitrogen species
G J Quinlan and JMC Gutteridge 263
PART 3: EXPERIMENTAL PATHOPHYSIOLOGICAL
MODELS
76 Isolated, perfused heart systems (Langendorf s model)
Hideo Kusuoka and Tusunehiko Kuzuya 269
77 Myocardial ischemia-reperfusion models in vivo
Shiro Hoshida 271
78 GI tract ischaemia-reperfusion model
Toshikazu Yoshikawa and Yuji Naito 275
79 Models of brain ischaemia
Masayasu Matsumoto 279
80 Isolated rat lung preparation
T W Evans and JMC Gutteridge 285
81 Cell culture model for oxidative stress
Keiichiro Suzuki 287
82 Atherosclerosis (oxidized LDL): preparation of oxidized
LDL/detection of cholesterol accumulation in macrophages
Masayuki Yokode and Toru Kita 289
83 Dicarbonyls and HB-EGF gene expression
Wenyi Che, Young Ho Koh, and Naoyuki Taniguchi 293
84 UV damage models
Yoshiki Miyachi 296
85 Model for ageing: longevity of Caenorhabditis elegans
Naoaki Ishii 300
86 Ovulation: superovulation and inhibition by SOD/in vitro
fertilization and SOD
Mutsuo Ishikawa 303
87 Scavenging NO in experimental animals
Takaaki Akaike and Yoichi Miyamoto 306
88 Experimental protocols for ROS and RNS: cyclic GMP
radioimmunoassay using brain slices
DaisukeOkada 311
xii
Contents
89 Oxidative stress models: E coli, cultured cells
Qiu-Mei Zhang, Akira Tachibana, and Shuji Yonei 316
90 Carcinogenesis model: progression models in vitro and in
vivo
Futoshi Okada and Masuo Hosokawa 320
91 Iron-overload model
Shinya Toyokuni 325
92 Copper-overload model
Keiichiro Suzuki 329
Index 333
xni
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| discipline | Biologie |
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| language | English Japanese |
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| spelling | Experimental protocols for reactive oxygen and nitrogen species ed. by Naoyuki Taniguchi ... 1. publ. Oxford u.a. Oxford Univ. Press 2000 XIX, 337 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Espèces réactives oxygénées Monoxyde d'azote Monoxyde d'azote ram Monoxyde da̓zote ram Oxygène actif ram Radicaux libres (chimie) - Effets physiologiques ram Radicaux libres - Effets physiologiques ram Radicaux libres - Physiologie Active oxygen Laboratory manuals Free radicals (Chemistry) Physiological effect Laboratory manuals Nitric oxide Laboratory manuals Radikal Chemie (DE-588)4076475-8 gnd rswk-swf Stickstoffmonoxid (DE-588)4183282-6 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Stickstoffmonoxid (DE-588)4183282-6 s Radikal Chemie (DE-588)4076475-8 s Labortechnik (DE-588)4123602-6 s DE-604 Taniguchi, Naoyuki Sonstige oth HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009426956&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Experimental protocols for reactive oxygen and nitrogen species Espèces réactives oxygénées Monoxyde d'azote Monoxyde d'azote ram Monoxyde da̓zote ram Oxygène actif ram Radicaux libres (chimie) - Effets physiologiques ram Radicaux libres - Effets physiologiques ram Radicaux libres - Physiologie Active oxygen Laboratory manuals Free radicals (Chemistry) Physiological effect Laboratory manuals Nitric oxide Laboratory manuals Radikal Chemie (DE-588)4076475-8 gnd Stickstoffmonoxid (DE-588)4183282-6 gnd Labortechnik (DE-588)4123602-6 gnd |
| subject_GND | (DE-588)4076475-8 (DE-588)4183282-6 (DE-588)4123602-6 |
| title | Experimental protocols for reactive oxygen and nitrogen species |
| title_auth | Experimental protocols for reactive oxygen and nitrogen species |
| title_exact_search | Experimental protocols for reactive oxygen and nitrogen species |
| title_full | Experimental protocols for reactive oxygen and nitrogen species ed. by Naoyuki Taniguchi ... |
| title_fullStr | Experimental protocols for reactive oxygen and nitrogen species ed. by Naoyuki Taniguchi ... |
| title_full_unstemmed | Experimental protocols for reactive oxygen and nitrogen species ed. by Naoyuki Taniguchi ... |
| title_short | Experimental protocols for reactive oxygen and nitrogen species |
| title_sort | experimental protocols for reactive oxygen and nitrogen species |
| topic | Espèces réactives oxygénées Monoxyde d'azote Monoxyde d'azote ram Monoxyde da̓zote ram Oxygène actif ram Radicaux libres (chimie) - Effets physiologiques ram Radicaux libres - Effets physiologiques ram Radicaux libres - Physiologie Active oxygen Laboratory manuals Free radicals (Chemistry) Physiological effect Laboratory manuals Nitric oxide Laboratory manuals Radikal Chemie (DE-588)4076475-8 gnd Stickstoffmonoxid (DE-588)4183282-6 gnd Labortechnik (DE-588)4123602-6 gnd |
| topic_facet | Espèces réactives oxygénées Monoxyde d'azote Monoxyde da̓zote Oxygène actif Radicaux libres (chimie) - Effets physiologiques Radicaux libres - Effets physiologiques Radicaux libres - Physiologie Active oxygen Laboratory manuals Free radicals (Chemistry) Physiological effect Laboratory manuals Nitric oxide Laboratory manuals Radikal Chemie Stickstoffmonoxid Labortechnik |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009426956&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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