Crystal structure of the macrophage infectivity potentiator protein, a major virulence factor from Legionella pneumophila:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
2000
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Jena, Univ., Diss., 2000 |
| Physical Description: | VII, 139 S. Ill., graph. Darst. : 31 cm |
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MARC
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| 245 | 1 | 0 | |a Crystal structure of the macrophage infectivity potentiator protein, a major virulence factor from Legionella pneumophila |c von Alan Riboldi-Tunnicliffe |
| 264 | 1 | |c 2000 | |
| 300 | |a VII, 139 S. |b Ill., graph. Darst. : 31 cm | ||
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| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Jena, Univ., Diss., 2000 | ||
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Record in the Search Index
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| adam_text |
INDEX
CONTENTS. PAGE.
1. INTRODUCTION
1
1.1 LEGIONELLAE
1
1.2 DISEASES CAUSED BY
LEGIONELLA 2
1.3 CELL INVASION
3
1.4 THE PHAGOSOME
4
1.5 VIRULENCE GENES
5
1.6 THE MACROPHAGE INFECTIVITY POTENTIATOR (MIP) PROTEIN 6
1.7 PEPTIDYL-PROLYL
CIS/TRANS ISOMERASES 8
1.7.1 CYCLOPHILINS 10
1.7.2 PARVULINS H
1.7.3 FKBPS
12
1.8 AIM OF THE WORK
16
2. MATERIALS AND METHODS
17
2.1 PROTEIN
17
2.2 ENZYME PURIFICATION
17
2.2.1 MUTANT PROTEIN PREPARATION 20
2.3 ENZYME CONCENTRATING 20
2.4 PROTEIN CONCENTRATION 21
2.5 CRYSTAL DENSITY MEASUREMENTS 21
2.6 MEASUREMENT METHOD FOR FLUORESCENCE SPECTRA OF ZINC 23
2.7 CRYSTALLISATION 24
2.7.1 WILD TYPE MIP 24
2.7.2 WILD TYPE MIP IN COMPLEX WITH FK506
25
2.7.3 MIP MUTANTS Y13 IF AND Y185F 25
2.8 PREPARATION OF CRYSTALS FOR DATA COLLECTION AT 100 K 25
2.9 DIFFRACTION DATA COLLECTION 26
2.10 STRUCTURE ANALYSIS 27
3. RESULTS
28
3.1 PREPARATION OF THE MIP PROTEIN FROM
LEGIONELLA PNEUMOPHILA 28
3.2 CRYSTALLISATION OF THE MIP PROTEIN 28
3.3 CHARACTERISATION OF MIP CRYSTALS
31
3.4 DATA COLLECTION
32
3.5 NON-FIOMORPHISM
33
3.6 DIMER OR MONOMER IN THE SYMMETRIC UNIT?
34
3.7 CRYSTAL DENSITY MEASUREMENTS 38
3.8 CALCULATION OF THE MOLECULAR WEIGHT OF AN ASYMMETRIC UNIT 38
I
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/960995838
INDEX
3.9 INITIAL ATTEMPTS TO SOLVE THE STRUCTURE 41
3.9.1 MOLECULAR REPLACEMENT AND EPMR 42
3.10 MAD DATA COLLECTION 44
3.11 LOCATION OF THE ZINC IONS 46
3.12 THE 2.4
A
STRUCTURE OF MIP SOLVED BY MAD 50
3.12.1 QUALITY OF THE STRUCTURE 53
3.13 CRYSTAL STRUCTURE OF MIP AT 1.8 A RESOLUTION 60
3.13.1 QUALITY OF THE STRUCTURE 64
3.14 CRYSTAL STRUCTURE OF MIP IN COMPLEX WITH FK506 AT 3.15
A
RESOLUTION 474
3.15 STRUCTURE OF THE MUTANT Y13 IF AT 2.8
A
RESOLUTION 78
3.15.1 QUALITY OF THE STRUCTURE 82
3.16 STRUCTURE OF THE MUTANT Y185F AT 2.9
A
RESOLUTION 84
3.16.1 QUALITY OF THE STRUCTURE 87
4. DISCUSSION
89
4.1 VIRULENCE FACTORS 89
4.2 SEQUENCE COMPARISON 91
4.2.1 CONSERVED PPIASE ACTIVE SITE RESIDUES 93
4.3 DIFFERENCES IN UNIT CELL DIMENSIONS 95
4.4 CRYSTAL PACKING 97
4.4.1 CRYSTAL CONTACTS 98
4.5 INTERACTIONS BETWEEN MIP AND FK506 99
4.6 Y131F MUTANT 103
4.7 Y185F MUTANT 105
5. SUMMARY
108
6. REFERENCES
109
7. APPENDIX
H |
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| id | DE-604.BV013705974 |
| illustrated | Illustrated |
| indexdate | 2024-10-09T18:09:50Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009366790 |
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| physical | VII, 139 S. Ill., graph. Darst. : 31 cm |
| publishDate | 2000 |
| publishDateSearch | 2000 |
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| spelling | Riboldi-Tunnicliffe, Alan 1970- Verfasser (DE-588)122749405 aut Crystal structure of the macrophage infectivity potentiator protein, a major virulence factor from Legionella pneumophila von Alan Riboldi-Tunnicliffe 2000 VII, 139 S. Ill., graph. Darst. : 31 cm txt rdacontent n rdamedia nc rdacarrier Jena, Univ., Diss., 2000 Hochschulschrift gtt Legionella pneumophila (DE-588)4481578-5 gnd rswk-swf Struktur-Aktivitäts-Beziehung (DE-588)4183784-8 gnd rswk-swf Röntgenstrukturanalyse (DE-588)4137203-7 gnd rswk-swf Membranproteine (DE-588)4130026-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Legionella pneumophila (DE-588)4481578-5 s Membranproteine (DE-588)4130026-9 s Struktur-Aktivitäts-Beziehung (DE-588)4183784-8 s Röntgenstrukturanalyse (DE-588)4137203-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009366790&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Riboldi-Tunnicliffe, Alan 1970- Crystal structure of the macrophage infectivity potentiator protein, a major virulence factor from Legionella pneumophila Hochschulschrift gtt Legionella pneumophila (DE-588)4481578-5 gnd Struktur-Aktivitäts-Beziehung (DE-588)4183784-8 gnd Röntgenstrukturanalyse (DE-588)4137203-7 gnd Membranproteine (DE-588)4130026-9 gnd |
| subject_GND | (DE-588)4481578-5 (DE-588)4183784-8 (DE-588)4137203-7 (DE-588)4130026-9 (DE-588)4113937-9 |
| title | Crystal structure of the macrophage infectivity potentiator protein, a major virulence factor from Legionella pneumophila |
| title_auth | Crystal structure of the macrophage infectivity potentiator protein, a major virulence factor from Legionella pneumophila |
| title_exact_search | Crystal structure of the macrophage infectivity potentiator protein, a major virulence factor from Legionella pneumophila |
| title_full | Crystal structure of the macrophage infectivity potentiator protein, a major virulence factor from Legionella pneumophila von Alan Riboldi-Tunnicliffe |
| title_fullStr | Crystal structure of the macrophage infectivity potentiator protein, a major virulence factor from Legionella pneumophila von Alan Riboldi-Tunnicliffe |
| title_full_unstemmed | Crystal structure of the macrophage infectivity potentiator protein, a major virulence factor from Legionella pneumophila von Alan Riboldi-Tunnicliffe |
| title_short | Crystal structure of the macrophage infectivity potentiator protein, a major virulence factor from Legionella pneumophila |
| title_sort | crystal structure of the macrophage infectivity potentiator protein a major virulence factor from legionella pneumophila |
| topic | Hochschulschrift gtt Legionella pneumophila (DE-588)4481578-5 gnd Struktur-Aktivitäts-Beziehung (DE-588)4183784-8 gnd Röntgenstrukturanalyse (DE-588)4137203-7 gnd Membranproteine (DE-588)4130026-9 gnd |
| topic_facet | Hochschulschrift Legionella pneumophila Struktur-Aktivitäts-Beziehung Röntgenstrukturanalyse Membranproteine |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009366790&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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