Studies on the cyclodextrin metabolism of Klebsiella oxytoca M5a1:
Saved in:
| Main Author: | |
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2000
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 76 S. Ill., graph. Darst. : 21 cm |
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MARC
| LEADER | 00000nam a2200000 c 4500 | ||
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| 001 | BV013705388 | ||
| 003 | DE-604 | ||
| 005 | 20010625 | ||
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| 008 | 010424s2000 gw ad|| m||| 00||| eng d | ||
| 016 | 7 | |a 960680454 |2 DE-101 | |
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| 035 | |a (DE-599)BVBBV013705388 | ||
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| 041 | 0 | |a eng | |
| 044 | |a gw |c DE | ||
| 049 | |a DE-29T |a DE-355 |a DE-188 | ||
| 100 | 1 | |a Raras, Tri Yudani M. |d 1965- |e Verfasser |0 (DE-588)122651944 |4 aut | |
| 245 | 1 | 0 | |a Studies on the cyclodextrin metabolism of Klebsiella oxytoca M5a1 |c by Tri Yudani M. Raras submitted |
| 264 | 1 | |c 2000 | |
| 300 | |a 76 S. |b Ill., graph. Darst. : 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a München, Univ., Diss., 2000 | ||
| 650 | 7 | |a Hochschulschrift |2 gtt | |
| 650 | 0 | 7 | |a Klebsiella oxytoca |0 (DE-588)4164081-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Stoffwechsel |0 (DE-588)4057699-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Cyclodextrine |0 (DE-588)4148527-0 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Klebsiella oxytoca |0 (DE-588)4164081-0 |D s |
| 689 | 0 | 1 | |a Cyclodextrine |0 (DE-588)4148527-0 |D s |
| 689 | 0 | 2 | |a Stoffwechsel |0 (DE-588)4057699-1 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009366485&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009366485 | |
Record in the Search Index
| _version_ | 1807229310404132864 |
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| adam_text |
CONTENTS
PAGE
A.
INTRODUCTION
1-11
1.
CYCLODEXTRINS,
CYCLODEXTRIN
DERIVATIVES
AND
THEIR
PRACTICAL
IMPORTANCE
1
2.
SYNTHESIS
AND
DEGRADATION
OF
CDS
3
3.
DEGRADATION
OF
STARCH
AND
METABOLISM
OF
CDS
BY
K.
OXYTOCA
6
4.
REGULATION
OF
MALTOSE
TRANSPORT
IN
E.
COLI
9
B.
MATERIALS
AND
METHODS
12
-
24
1.
STRAINS,
PLASMIDS
AND
OLIGONUCLEOTIDES
12
2.
ENZYMES
AND
CHEMICALS
15
3.
MEDIA
AND
GROWTH
CONDITIONS
15
4.
RECOMBINANT
DNA
TECHNIQUES
16
5.
BIOCHEMICAL
PROCEDURES
16
5.1.
STANDARD
METHODS
16
5.2.
ENZYME
ASSAYS
17
5.2.1.
DETERMINATION
OF
0-GALACTOSIDASE
ACTIVITY
17
5.2.2.
ASSAY
FOR
ACETYLTRANSFERASE
ACTIVITY
OF
CYMB
17
5.2.2.1.
IN
VITRO
ASSAY
17
'
5.2.2.2.
IN
VIVO
ASSAY
17
5.2.3.
ASSAY
OF
DEGRADATION
OF
CD
DERIVATIVES
BY
CYMH
18
5.2.4.
PREPARATION
OF
METHYL-A-CD
HYDROLYSIS
PRODUCT
FOR
MASS
SPECTROMETRY
ANALYSIS
18
5.3.
OVERPRODUCTION
AND
PROTEIN
PURIFICATION
19
5.3.1.
CYCLODEXTRINASE
(CYMH)
19
5.3.2.
CYMB
19
5.3.2.1.
CONSTRUCTION
OF
PLASMID
PDRB.029
19
5.3.2.2.
PURIFICATION
OF
CYMB-TRX
FUSION
19
5.3.3.
PURIFICATION
OF
CYMB
USING
A
"BATCH"METHOD
20
6.
PHYSIOLOGICAL
PROCEDURES
20
6.1.
TRANSPORT
ASSAYS
20
6.2.
ISOLATION
OF
THE
BACTERIA
DEGRADING
MODIFIED
CDS
21
6.3.
MORPHOLOGICAL
STUDIES
OF
STRAIN
REGB/PCYMJ
21
7.
MOLECULAR
BIOLOGICAL
TECHNIQUES
21
7.1.
CELLULAR
LOCALISATION
OF
CYMJ
PROTEIN
21
7.2.
PRODUCTION
OF
CYMB
ANTISERA
22
7.3.
IMMUNOBLOTTING
23
8.
CONSTRUCTION
OF
A
K.
OXYTOCA
CYMB
CHROMOSOMAL
DELETION
MUTANT
23
9.
DATA
ANALYSIS
24
C.
RESULTS
25
-
61
1.
INVESTIGATION
OF
THE
UPTAKE
OF
CD
DERIVATIVES
BY
K.
OXYTOCA
25
1.1.
GROWTH
OF
K.
OXYTOCA
ON
CD
DERIVATIVES
25
1.2.
CLEAVAGE
OF
CD
DERIVATIVES
BY
CYMH
26
1.2.1.
PURIFICATION
OF
CYMH
26
1.2.2.
ASSAY
OF
CYMH
FOR
THE
CAPACITY
TO
CLEAVE
CD
DERIVATIVES
27
1.3.
MODIFIED
CDS
CAN
NOT
INDUCE
THE
CYM
OPERON
31
1.4.
COMPETITION
BETWEEN
A-CD
AND
CD
DERIVATIVES
TO
BIND
TO
THE
PERIPLASMIC
BINDING
PROTEIN
33
1.5.
THE
DEGRADATION
OF
CD
DERIVATIVES
BY
NATURAL
ISOLATES
34
2.
CHARACTERISATION
OF
CYMB
37
2.1.
IN
VIVO
TEST
FOR
ACETYLTRANSFERASE
ACTIVITY
OF
CYMB
38
2.2.
OVERPRODUCTION
AND
PURIFICATION
OF
CYMB
39
2.2.1.
CONSTRUCTION
OF
PLASMID
PDRB.029
41
2.2.2.
PURIFICATION
OF
THE
CYMB
FUSION
PROTEIN
43
2.2.3.
IN
VITRO
ASSAY
FOR
ACETYLTRANSFERASE
ACTIVITY
OF
CYMB
44
2.3.
CHARACTERISATION
OF
CYMB
DELETION
MUTANT
45
2.3.1.
CONSTRUCTION
OF
A
CYMB
DELETION
MUTANT
46
2.3.2.
GROWTH
OF
THE
K.
OXYTOCA
\CYMB
MUTANT
48
3.
PHYSIOLOGICAL
STUDY
ON
CYMJ
49
3.1.
SUBCELLULAR
LOCALISATION
OF
CYMJ
49
3.2.
CYMJ
IS
A
PUTATIVE
REPRESSOR
AND
THE
REPRESSION
EFFECT
OF
CYMJ
CAN
BE
RELASED
BY
ADDITION
OF
THE
INDUCER
51
3.3.
INHIBITION
OF
CELL
SEPTATION
UPON
OVEREXPRESSION
OF
CYMJ
53
3.4.
OVEREXPRESSION
OF
CYMJ
IN
K.
OXYTOCA
CYM
DELETION
MUTANT
55
3.5.
OVEREXPRESSION
OF
CYMJ
FROM
A
PLASMID
CARRYING
A
KANAMYCIN
RESISTANCE
GENE
58
3.5.1.
IN
A
HOMOLOGOUS
SYSTEM
58
3.5.2.
IN
A
HETEROLOGOUS
SYSTEM
59
3.6.
CAMP/CAP
ENHANCE
CYMJ
REPRESSION
ACTIVITY
59
3.7.
THE
ANTIBIOTIC
SUSCEPTIBILITY
OF
STRAINS
OVEREXPRESSING
CYMJ
60
D.
DISCUSSION
62
-
69
1.
CYMB
62
2.
THE
REGULATION
OF
THE
CYM
OPERON
63
3.
PHYSIOLOGICAL
EFFECT
OF
THE
OVEREXPRESSION
OF
CYMJ
67
4.
DEGRADATION
OF
CD
DERIVATIVES
IN
K.
OXYTOCA
68
5.
SOIL
SAMPLE
SCREENING
FOR
ISOLATION
OF
BACTERIA
ABLE
TO
DEGRADE
MODIFIED
CDS
69
E.
SUMMARY
70
F.
REFERENCES
71-76 |
| any_adam_object | 1 |
| author | Raras, Tri Yudani M. 1965- |
| author_GND | (DE-588)122651944 |
| author_facet | Raras, Tri Yudani M. 1965- |
| author_role | aut |
| author_sort | Raras, Tri Yudani M. 1965- |
| author_variant | t y m r tym tymr |
| building | Verbundindex |
| bvnumber | BV013705388 |
| ctrlnum | (OCoLC)47303141 (DE-599)BVBBV013705388 |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV013705388 |
| illustrated | Illustrated |
| indexdate | 2024-08-13T00:16:16Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009366485 |
| oclc_num | 47303141 |
| open_access_boolean | |
| owner | DE-29T DE-355 DE-BY-UBR DE-188 |
| owner_facet | DE-29T DE-355 DE-BY-UBR DE-188 |
| physical | 76 S. Ill., graph. Darst. : 21 cm |
| publishDate | 2000 |
| publishDateSearch | 2000 |
| publishDateSort | 2000 |
| record_format | marc |
| spelling | Raras, Tri Yudani M. 1965- Verfasser (DE-588)122651944 aut Studies on the cyclodextrin metabolism of Klebsiella oxytoca M5a1 by Tri Yudani M. Raras submitted 2000 76 S. Ill., graph. Darst. : 21 cm txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2000 Hochschulschrift gtt Klebsiella oxytoca (DE-588)4164081-0 gnd rswk-swf Stoffwechsel (DE-588)4057699-1 gnd rswk-swf Cyclodextrine (DE-588)4148527-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Klebsiella oxytoca (DE-588)4164081-0 s Cyclodextrine (DE-588)4148527-0 s Stoffwechsel (DE-588)4057699-1 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009366485&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Raras, Tri Yudani M. 1965- Studies on the cyclodextrin metabolism of Klebsiella oxytoca M5a1 Hochschulschrift gtt Klebsiella oxytoca (DE-588)4164081-0 gnd Stoffwechsel (DE-588)4057699-1 gnd Cyclodextrine (DE-588)4148527-0 gnd |
| subject_GND | (DE-588)4164081-0 (DE-588)4057699-1 (DE-588)4148527-0 (DE-588)4113937-9 |
| title | Studies on the cyclodextrin metabolism of Klebsiella oxytoca M5a1 |
| title_auth | Studies on the cyclodextrin metabolism of Klebsiella oxytoca M5a1 |
| title_exact_search | Studies on the cyclodextrin metabolism of Klebsiella oxytoca M5a1 |
| title_full | Studies on the cyclodextrin metabolism of Klebsiella oxytoca M5a1 by Tri Yudani M. Raras submitted |
| title_fullStr | Studies on the cyclodextrin metabolism of Klebsiella oxytoca M5a1 by Tri Yudani M. Raras submitted |
| title_full_unstemmed | Studies on the cyclodextrin metabolism of Klebsiella oxytoca M5a1 by Tri Yudani M. Raras submitted |
| title_short | Studies on the cyclodextrin metabolism of Klebsiella oxytoca M5a1 |
| title_sort | studies on the cyclodextrin metabolism of klebsiella oxytoca m5a1 |
| topic | Hochschulschrift gtt Klebsiella oxytoca (DE-588)4164081-0 gnd Stoffwechsel (DE-588)4057699-1 gnd Cyclodextrine (DE-588)4148527-0 gnd |
| topic_facet | Hochschulschrift Klebsiella oxytoca Stoffwechsel Cyclodextrine |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009366485&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT rarastriyudanim studiesonthecyclodextrinmetabolismofklebsiellaoxytocam5a1 |