Investigations on the in vitro developmental competence of cumulus oocyte complexes recovered from calves ex vivo and post mortem:
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| Format: | Book |
| Language: | English |
| Published: |
2000
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Leipzig, Univ., Diss., 2000 |
| Physical Description: | 97, XXI S. Ill., graph. Darst. : 21 cm |
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MARC
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| 001 | BV013705230 | ||
| 003 | DE-604 | ||
| 005 | 20030102 | ||
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| 008 | 010424s2000 gw ad|| m||| 00||| eng d | ||
| 016 | 7 | |a 960293566 |2 DE-101 | |
| 035 | |a (OCoLC)614710877 | ||
| 035 | |a (DE-599)BVBBV013705230 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c DE | ||
| 049 | |a DE-355 | ||
| 084 | |a 610 |2 sdnb | ||
| 100 | 1 | |a Amer, Hussein Ahmed Hussein |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Investigations on the in vitro developmental competence of cumulus oocyte complexes recovered from calves ex vivo and post mortem |c submitted by Hussein Ahmed Hussein Amer |
| 264 | 1 | |c 2000 | |
| 300 | |a 97, XXI S. |b Ill., graph. Darst. : 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Leipzig, Univ., Diss., 2000 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009366438&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009366438 | |
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| _version_ | 1807504492574277632 |
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CONTENTS
1
INTRODUCTION
1
2
REVIEW
OF
LITERATURE
3
2.1
OOGENESIS
AND
FOLLICULOGENESIS
3
2.1.1
OOGENESIS
3
2.1.1.1
GROWTH
OF
PRIMORDIAL
GERM
CELLS
TO
OOGONIA
3
2.1.1.2
GROWTH
PHASES
AND
GENERATION
OF
OOCYTE
3
2.1.1.3
OOCYTE
MATURATION
4
2.1.2
FOLLICULOGENESIS
6
22
FOLLICULAR
DYNAMICS
IN
BOVINE
7
2.2.1
FOLLICULAR
DYNAMICS
IN
ADULT
BOVINE
7
2.2.2
FOLLICULAR
DYNAMICS
IN
PREPUBERTAL
BOVINE
8
2.3
IN
VITRO
PRODUCTION
OF
BOVINE
EMBRYOS
9
2.3
1
METHODS
FOR
THE
RECOVERY
OF
PREPUBERTAL
CUMULUS
OOCYTE
COMPLEXES
9
2.3.1.1
POST
MORTEM
RECOVERY
9
2.3.1.2
EX
VIVO
RECOVERY
OF
THE
CUMULUS
OOCYTE
COMPLEXES
11
2
3.2
IN
VITRO
MATURATION
(IVM)
14
2.3.2.1
MATURATION
MEDIA
14
2.3.2.2
PROTEINS
AND
NUTRITIVE
SUBSTANCES
ADDITIVES
15
2
3
2.3
GONADOTROPINS,
STEROIDS
AND
GROWTH
FACTORS
ADDITIVES
16
2.3.2.4
SOMATIC
CELLS
SUPPORT
17
23
3
IN
VITRO
CAPACITATION
AND
FERTILIZATION
19
2.3.3.1
IN
VITRO
CAPACITATION
19
23.3.1.1
SPERM
PRESERVATION
AND
BULL
VARIABILITY
20
2.3.3.1
2
MEDIA
AND
MEDIA
ADDITIVES
21
2
3.3.1.3
METHODS
OF
SPERM
SEPARATION
22
2.3.3.2
IN
VITRO
FERTILIZATION
(IVF)
23
2.3.3.2.1
MEDIA
AND
MEDIA
ADDITIVES
23
23.3.2.2
SPERM
CONCENTRATION
AND
PERIOD
OF
CO-INCUBATION
24
2.3.4
IN
VITRO
CULTURE
(IVC)
OF
BOVINE
EMBRYOS
25
2.3.4.1
EARLY
EMBRYONIC
DEVELOPMENT
UP
TO
THE
BLASTOCYST
STAGE
25
2.3
4.2
EFFECT
OF
MEDIA
AND
MEDIA
ADDITIVES
26
2.3.43
EFFECT
OF
SOMATIC
CELLS
SUPPORT
AND
CONDITIONED
MEDIA
28
2.4
DEVELOPMENTAL
COMPETENCE
OF
THE
CUMULUS
OOCYTE
COMPLEXES
AND
EMBRYOS
30
2
4.1
DEVELOPMENTAL
COMPETENCE
RELATIVE
TO
THE
SIZE
OF
FOLLICLES
30
2.4.1.1
THE
ABILITY
OF
CUMULUS
OOCYTE
COMPLEXES
TO
MATURE
IN
VITRO
30
2.4.1.2
THE
ABILITY
OF
CUMULUS
OOCYTE
COMPLEXES
TO
FERTILIZE
IN
VITRO
31
2.4.1.3
EMBRYONIC
DEVELOPMENT
IN
VITRO
32
2.4
2
DEVELOPMENTAL
COMPETENCE
RELATIVE
TO
THE
AGE
OF
ANIMAL
34
2.4.2.1
THE
ABILITY
OF
CUMULUS
OOCYTE
COMPLEXES
TO
MATURE
IN
VITRO
34
2
4.2
2
THE
ABILITY
OF
CUMULUS
OOCYTE
COMPLEXES
TO
FERTILIZE
IN
VITRO
34
2.4.2.3
EMBRYONIC
DEVELOPMENT
IN
VITRO
35
3
OWN
EXPERIMENTS
38
3.1
MATERIAL
AND
METHODS
38
3.1.1
MATERIAL
38
3.1.1
1
ANIMALS
FOR
LAPAROSCOPICAL
OVUM
PICK-UP
(L-OPU)
38
3.1.1.2
SLAUGHTERHOUSE
MATERIAL
39
3
1
2
METHODS
39
3.1.2.1
COLLECTION
OF
THE
CUMULUS
OOCYTE
COMPLEXES
39
3.1
2.1.1
COLLECTION
OF
THE
PREPUBERTAL
CUMULUS
OOCYTE
COMPLEXES
BY
LAPAROSCOPY
39
3.1.2.1.2
COLLECTION
OF
PREPUBERTAL
AND
ADULT
CUMULUS
OOCYTE
COMPLEXES
FROM
SLAUGHTERED
ANIMALS
43
3
1
2.2
COLLECTION
OF
GRANULOSA
CELLS
44
3
1.2.3
COLLECTION
OF
THE
BOVINE
OVIDUCTAL
EPITHELIAL
CELLS
45
3.1.2
4
IN
VITRO
PRODUCTION
OF
BOVINE
EMBRYOS
45
3.1.2.4.1
ASSESSMENT
THE
QUALITY
OF
THE
RECOVERED
CUMULUS
OOCYTE
COMPLEXES
45
3
1.2.4
2
IN
VITRO
MATURATION
46
3.1.2.4.3
IN
VITRO
CAPACITATION
AND
FERTILIZATION
47
3.1.2.4.4
IN
VITRO
CULTURE
OF
THE
EARLY
PRODUCED
BOVINE
EMBRYOS
48
3
1.2.5
STAINING
OF
OOCYTES,
ZYGOTES
AND
EMBRYOS
49
3.1.2.6
PARAMETERS
TO
CHARACTERIZE
THE
IN
VITRO
PRODUCTION
OF
EMBRYOS
49
3.1.2.7
STATISTICAL
ANALYSIS
50
3.2
RESULTS
51
3
2.1
RESULTS
OF
LAPAROSCOPICAL
OVUM
PICK-UP
51
3
2.1.1
FOLLICULAR
PUNCTION
AND
OOCYTES
ASPIRATION
51
3
2.2
QUALITY
OF
THE
FOLLICLES
DURING
LAPAROSCOPY
52
322
1
COMPARISON
OF
THE
NUMBER
OF
FOLLICLES
AND
FOLLICULAR
DIAMETER
52
3.2.2.2
COMPARISON
OF
THE
NUMBER
OF
FOLLICLES
AND
THE
AGE
53
3
2.2
3
COMPARISON
OF
THE
NUMBER
OF
FOLLICLES
AND
THE
FREQUENCY
OF
PUNCTION
54
3.2.3
FOLLICULAR
PUNCTION
AND
RECOVERY
RATE
57
3.2.3
1
NUMBER
OF
THE
PUNCTURED
FOLLICLES
AND
RECOVERY
RATE
57
3
2.3.2
COMPARISON
THE
QUALITY
OF
OOCYTES
AND
FOLLICULAR
DIAMETER
57
3
2.4
IN
VITRO
PRODUCTION
OF
BOVINE
EMBRYOS
GENERATED
FROM
PREPUBERTAL
COCS
RETRIEVED
BY
LAPAROSCOPY
62
3.2.5
COMPARISON
OF
THE
IN
VITRO
PRODUCTION
OF
BOVINE
EMBRYOS
GENERATED
FROM
THE
COCS
RECOVERED
POST
MORTEM
TO
THE
FOLLICULAR
DIAMETER,
AGE
OF
THE
DONOR
AND
THE
SOURCE
OF
GRANULOSA
CELLS
62
3.2.6
EMBRYONIC
CELL
NUMBER
44
TO
48
HPF
AND
THE
RATE
OF
BLASTOCYSTS
PRODUCED
AT
DAY
9
OF
CULTURE
69
3.2.7
EVALUATION
THE
RATE
OF
POLYSPERMY
74
3.2.8
NUMBER
OF
THE
BLASTOMERS
OF
DAY
9
BLASTOCYSTS
75
4
DISCUSSION
76
4.1
RECOVERY
OF
PREPUBERTAL COCS
BY
LAPAROSCOPIC
OVUM
PICK-UP
76
4.2
DEVELOPMENTAL
COMPETENCE
OF
PREPUBERTAL
COCS
RELATIVE
TO
THE
SIZE
OF
FOLLICLE
AND
THE
AGE
OF
DONOR
81
4.3
DEVELOPMENTAL
COMPETENCE
OF
PREPUBERTAL
COCS
FROM
FOLLICLES
OF
DIFFERENT
SIZES
RELATIVE
TO
SOURCE
OF
GRANULOSA
CELLS
88
5
SUMMARY
92
5.1
ZUSAMMENFASSUNG
95
6
REFERENCES
I
7
APPENDIX
XIX |
| any_adam_object | 1 |
| author | Amer, Hussein Ahmed Hussein |
| author_facet | Amer, Hussein Ahmed Hussein |
| author_role | aut |
| author_sort | Amer, Hussein Ahmed Hussein |
| author_variant | h a h a hah haha |
| building | Verbundindex |
| bvnumber | BV013705230 |
| ctrlnum | (OCoLC)614710877 (DE-599)BVBBV013705230 |
| discipline | Medizin |
| format | Book |
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| indexdate | 2024-08-16T01:10:10Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009366438 |
| oclc_num | 614710877 |
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| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 97, XXI S. Ill., graph. Darst. : 21 cm |
| publishDate | 2000 |
| publishDateSearch | 2000 |
| publishDateSort | 2000 |
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| spelling | Amer, Hussein Ahmed Hussein Verfasser aut Investigations on the in vitro developmental competence of cumulus oocyte complexes recovered from calves ex vivo and post mortem submitted by Hussein Ahmed Hussein Amer 2000 97, XXI S. Ill., graph. Darst. : 21 cm txt rdacontent n rdamedia nc rdacarrier Leipzig, Univ., Diss., 2000 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009366438&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Amer, Hussein Ahmed Hussein Investigations on the in vitro developmental competence of cumulus oocyte complexes recovered from calves ex vivo and post mortem |
| subject_GND | (DE-588)4113937-9 |
| title | Investigations on the in vitro developmental competence of cumulus oocyte complexes recovered from calves ex vivo and post mortem |
| title_auth | Investigations on the in vitro developmental competence of cumulus oocyte complexes recovered from calves ex vivo and post mortem |
| title_exact_search | Investigations on the in vitro developmental competence of cumulus oocyte complexes recovered from calves ex vivo and post mortem |
| title_full | Investigations on the in vitro developmental competence of cumulus oocyte complexes recovered from calves ex vivo and post mortem submitted by Hussein Ahmed Hussein Amer |
| title_fullStr | Investigations on the in vitro developmental competence of cumulus oocyte complexes recovered from calves ex vivo and post mortem submitted by Hussein Ahmed Hussein Amer |
| title_full_unstemmed | Investigations on the in vitro developmental competence of cumulus oocyte complexes recovered from calves ex vivo and post mortem submitted by Hussein Ahmed Hussein Amer |
| title_short | Investigations on the in vitro developmental competence of cumulus oocyte complexes recovered from calves ex vivo and post mortem |
| title_sort | investigations on the in vitro developmental competence of cumulus oocyte complexes recovered from calves ex vivo and post mortem |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009366438&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT amerhusseinahmedhussein investigationsontheinvitrodevelopmentalcompetenceofcumulusoocytecomplexesrecoveredfromcalvesexvivoandpostmortem |