Plant cell biology:
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| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Oxford
Oxford Univ. Press
2001
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| Edition: | 2. ed. |
| Series: | The practical approach series
250 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XX, 338 S. Ill., graph. Darst. |
| ISBN: | 0199638667 |
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MARC
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Record in the Search Index
| _version_ | 1804128475333787648 |
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| adam_text | PLANT CELL BIOLOGY SECOND EDITION EDITED BY CHRIS HAWES RESEARCH SCHOOL
OF BIOLOGICAL AND MOLECULAR SCIENCES, OXFORD BROOKES UNIVERSITY, OXFORD
0X3 OBP, UK AND BEATRICE SATIAT-JEUNEMAITRE INSTITUT DES SCIENCES
VEGETALES, CNRS UPR40, 91198 GIF-SUR-YVETTE, FRANCE TECHNISCHE
UNIVERSITY DARMSTADT FACHBEREICHIO-BIOLOGIE * BIB I I O T H E K *
SCHNITTSPAHNSTRABE 10 D-64287 DARMSTADT INV.-NR. OXPORD UNIVERSITY PRESS
CONTENTS LIST OF PROTOCOLS PAGE XV ABBREVIATIONS XIX 1 INTRODUCTION TO
OPTICAL MICROSCOPY FOR PLANT CELL BIOLOGY I P.J.SHAW 1 INTRODUCTION 1 2
EXPLANATION OF TERMS 4 3 RECORDING IMAGES 7 IMAGE RESOLUTION 7 FILM
RECORDING 8 ELECTRONIC CAMERAS 9 4 MICROSCOPE IMAGING MODES 10 BRIGHT
FIELD IMAGING 10 PHASE CONTRAST 12 DIFFERENTIAL INTERFERENCE CONTRAST
(NOMARSKI) 14 DARK FIELD 16 EPIFLUORESCENCE AND REFLECTED LIGHT
MICROSCOPY 17 5 CONFOCAL AND 3D MICROSCOPY 19 THE PROBLEM OF
OUT-OF-FOCUS LIGHT 19 THE CONFOCAL PRINCIPLE: EXPLANATION BY RAY OPTICS
20 PRACTICAL CONFOCAL MICROSCOPES 21 IMAGING AND THE POINT SPREAD
FUNCTION 23 DECONVOLUTION 24 TWO PHOTON IMAGING 26 6 COMPARISON OF
CONVENTIONAL, WIDE-FIELD FLUORESCENCE IMAGING WITH CONFOCAL FLUORESCENCE
IMAGING 27 NOISE AND RESOLUTION 27 WHEN SHOULD CONFOCAL MICROSCOPY BE
USED? 29 OBJECTIVE LENSES FOR CONFOCAL IMAGING 30 7 SPECIMEN PREPARATION
FOR CONFOCAL IMAGING 30 REFERENCES 33 2 FLUORESCENT PROBES FOR LIVING
PLANT CELLS 35 MARK FLICKER, ANDREW PARSONS, MONIKA TLALKA, ELISON
BLANCAFLOR, SIMON GILROY, ANDREAS MEYER, AND CHRISTOPH PLIETH 1
INTRODUCTION 35 VN CONTENTS 2 SELECTING PROBES WITH HIGH BRIGHTNESS 35
SPECTRAL CONSIDERATIONS 37 3 FLUORESCENCE LIFETIME IMAGING MICROSCOPY
(FLIM) 38 4 FLUORESCENCE POLARIZATION ANISOTROPY 38 5 FLUORESCENCE
RESONANCE ENERGY TRANSFER (FRET) 38 6 PHOTOBLEACHING AND
FLUORESCENCE REDISTRIBUTION AFTER PHOTOBLEACHING (FRAP) 39 7
OPTIMIZATION OF FLUORESCENT SYSTEMS FOR LIVE CELL IMAGING 40 SELECTION
OF THE EXCITATION WAVELENGTH 41 THE DICHROIC MIRROR 41 SELECTION OF THE
EMISSION WAVELENGTH 41 CHOICE OF MEASUREMENT SYSTEM 42 8 SECURING THE
SPECIMEN FOR MICROSCOPY 43 9 PERFUSION SYSTEMS 45 10 LOADING STRATEGIES
FOR PLANT CELLS 45 EXTRACELLULAR AND PERMEANT INTRACELLULAR DYES 46
ESTER LOADING 47 LOW PH LOADING 48 CUTINASE PRE-TREATMENT AND LOW PH
LOADING 49 ELECTROPORATION 50 LOADING VIA DETERGENT PERMEABILIZATION 50
LOADING TISSUES WITH PHLOEM-MOBILE PROBES 50 11 INTRACELLULAR DYE
CONCENTRATIONS, VIABILITY, AND TOXICITY 53 12 SELECTION AND USE OF
FLUORESCENT PROBES 54 VITAL STAINS 55 MORTAL STAINS 56 CELL PERMEANT
NUCLEAR STAINS 57 CHLOROPLASTS 57 MITOCHONDRIA 58 * VACUOLES 60
ENDOPLASMIC RETICULUM 62 GOLGI 62 CYTOSKELETON 63 THE PLASMA MEMBRANE
AND ENDOCYTOSIS 64 THE CELL WALL 65 13 PHYSIOLOGICAL PROBES 66 CALCIUM
66 MEASUREMENT OF APOPLASTIC, CYTOPLASMIC, AND VACUOLAR PH 72 POTASSIUM
76 ALUMINIUM 76 MEASUREMENT OF CYTOPLASMIC GLUTATHIONE LEVELS 77
REACTIVE OXYGEN SPECIES 77 14 DATA ANALYSIS 78 ATTENUATION CORRECTION
FOR OPTICAL SECTIONS DEEP INTO TISSUES 80 ACKNOWLEDGEMENTS 81 REFERENCES
81 3 FLOW CYTOMETRY 85 TEODORO COBA DE LA PENA AND SPENCER BROWN 1
INTRODUCTION 85 VM CONTENTS 2 CYTOMETRY DEMONSTRATED THROUGH CELL CYCLE
ANALYSES 86 HOW TO UNDERSTAND MONOPARAMETRIC DNA HISTOGRAMS 87
DEVELOPING MULTIPARAMETRIC DNA HISTOGRAMS AND IMMUNOFLUORESCENCE 90
EXTRACTING INTACT PLANT NUCLEI 91 WHICH DNA FLUOROCHROME IS
APPROPRIATE? 94 RUNNING AND READING THE CYTOMETER 94 BRDU INCORPORATION
TO IDENTIFY DNA SYNTHESIS BY FLUORESCENCE QUENCHING 95 3 THE PARTICULAR
APPLICATION OF GENOME SIZE CALCULATION AND DNA PLOIDY ^ ! TERMINOLOGY
99 INTERNAL OR EXTERNAL STANDARDS 99 CALCULATING BASE COMPOSITION 100 4
SORTING OF PROTOPLASTS AND CELLULAR ORGANELLES 101 5 TESTS FOR CELL
VIABILITY DURING FUNCTIONAL ASSAYS 1 04 6 CONCLUSION 104
ACKNOWLEDGEMENTS 104 REFERENCES 105 4 TRANSIENT EXPRESSION, A TOOL TO
ADDRESS QUESTIONS IN PLANT CELL BIOLOGY 107 JANE L. HADLINGTON
ANDJIIRGEN DENECKE 1 INTRODUCTION 107 2 CURRENT METHODS OF TRANSIENT
EXPRESSION 108 NAKED DNA TRANSFER 108 BIOLOGICAL VECTORS 1 08 3
APPLICATION OF TRANSIENT EXPRESSION 109 PROMOTER ANALYSIS 109 CELL
BIOLOGY AND BIOCHEMISTRY * 110 4 PRACTICAL CONSIDERATIONS FOR CELL
BIOLOGISTS 111 NAKED DNA TRANSFER 112 MEASUREMENT OF PROTEIN SECRETION
AND CELL RETENTION 116 LARGE SCALE TRANSIENT EXPRESSION FOR CELL
FRACTIONATION 118 SPECIALIZED APPLICATIONS 122 5 CONCLUSIONS 124
REFERENCES 124 5 THE GREEN FLUORESCENT PROTEIN (GFP) AS REPORTER IN
PLANT CELLS 127 JEAN-MARC NEUHAUS AND PETRA BOEVINK 1 INTRODUCTION 127 2
THE GREEN FLUORESCENT PROTEIN 127 STRUCTURE 127 GFP VARIANTS 128 3 GFP
AS A REPORTER FOR GENE EXPRESSION 128 4 GFP AS A REPORTER FOR PROTEIN
LOCATION 129 CYTOPLASM AND NUCLEUS 129 CHLOROPLASTS AND MITOCHONDRIA 130
SECRETORY PATHWAY 130 VIRAL PROTEINS 131 IX CONTENTS 5 TRANSFORMATION
METHODS 131 PEG-MEDIATED TRANSIENT EXPRESSION IN PROTOPLASTS 131
AGROBACTERIUM-MEDIATED TRANSIENT EXPRESSION IN PLANTA 134 VIRUS-MEDIATED
TRANSIENT EXPRESSION 136 6 VISUALIZATION AND MICROSCOPY OF GFP 140 7
FUTURE PERSPECTIVES 141 REFERENCES 141 6 MICROINJECTION 143 MICHPEL
KNOBLAUCH 1 INTRODUCTION 143 2 EQUIPMENT 143 ENVIRONMENT AND
INJECTION-TABLE 143 MICROSCOPE 144 OBJECTIVES 144 GLASS CAPILLARIES 145
TIP PULLER 145 MICROMANIPULATOR 146 3 INJECTION TECHNIQUES 146
IONTOPHORESIS 146 PRESSURE INJECTION 146 THE GALINSTAN EXPANSION
FEMTOSYRINGE (GEF) 147 4 CELL TYPES 149 EPIDERMAL CELLS 149 GUARD CELLS
AND TRICHOMES 150 MESOPHYLL CELLS 150 GROUND PARENCHYMA CELLS 151 SIEVE
ELEMENTS AND COMPANION CELLS 151 ALGAE 152 BACTERIA AND ORGANELLES 1 53
PLANT TISSUE CULTURES 153 5 MATERIAL SUITABLE FOR INJECTION 154
FLUOROCHROMES 154 DEXTRAN CONJUGATES 154 PROTEINS AND ANTIBODIES 155
NUCLEIC ACIDS 155 6 TIPS TO MAKE LIFE EASIER 155 THE CHUCK 155 SYRINGE
TO DELETE AIR BUBBLES 156 FLEXIBLE FUSED SILICA CAPILLARIES 156 PETRI
DISHES 157 REFERENCES 157 7 MICROMANIPUIATION BY LASER MICROBEAM AND
OPTICAL TWEEZERS 159 KARL OTTO GREULICH 1 INTRODUCTION 159 2 WHAT ARE
LASER MICROTOOLS? 159 CONTENTS 3 PHYSICAL BACKGROUND 160 GENERATING
EXTREME HEAT 160 WHY CAN LIGHT BE USED TO MOVE MICROSCOPIC OBJECTS? 160
4 HOW TO BUILD LASER MICROTOOLS 161 THE CHOICE OF LASERS 161 BUILDING A
LASER MICROBEAM OR OPTICAL TWEEZERS 162 5 APPLICATIONS OF LASER
MICROBEAMS IN PLANT BIOLOGY 163 LASER-INDUCED MICROINJECTION 163
ABLATION TO STUDY CELL FATE DURING PLANT DEVELOPMENT 164 PROTOPLAST
FUSION 165- : PREPARATION OF CELL MEMBRANES FROM ROOT HAIRS 165 6
APPLICATIONS OF OPTICAL TWEEZERS TO PLANT BIOLOGY 166 CAPTURING
SUBCELLULAR ORGANELLES FOR INSPECTION 166 SIMULATING MICROGRAVITY 167 7
CONCLUSION 168 REFERENCES 168 8 ELECTROPHYSIOLOGICAL METHODS: MONITORING
EXO- AND ENDOCYTOSIS IN REAL TIME IZI GERHARD THIEL, JENS-UWE SUTTER,
AND ULRIKE HOTNANN 1 INTRODUCTION 171 2 THEORETICAL BACKGROUND 171 THE
MEMBRANE IS EQUIVALENT TO A CAPACITOR 171 A CELL AS AN EQUIVALENT
CIRCUIT 172 3 TECHNIQUES FOR THE MEASUREMENT OF MEMBRANE CAPACITANCE 172
SQUARE-WAVE STIMULATION: TIME-DOMAIN TECHNIQUE 1 73 SAW-TOOTH
STIMULATION 174 CAPACITANCE CANCELLATION 1 74 SINUSOIDAL EXCITATION 174
4 CAPACITANCE MEASUREMENTS AS AN ASSAY FOR EXO- AND ENDOCYTOSIS:
PRACTICAL CONSIDERATIONS 175 WHAT KIND OF CELLS CAN BE EXAMINED? 176
ESTIMATION OF THE SPECIFIC CAPACITANCE 1 76 RECORDING OF SINGLE FUSION
AND FISSION EVENTS 176 WHAT KIND OF INFORMATION CAN BE EXTRACTED FROM
THE MEASUREMENTS? 1 79 MACROSCOPIC MEASUREMENT OF MEMBRANE CAPACITANCE
181 ACKNOWLEDGEMENTS 186 REFERENCES 187 9 PLANT HISTOLOGY 189 JACKIE
SPENCE 1 INTRODUCTION 189 2 CONVENTIONAL CHEMICAL FIXATION METHODS 189 3
CONVENTIONAL EMBEDDING METHODS AND SECTIONING 191 EMBEDDING IN A MATRIX
1 92 FROZEN SECTIONS 194 XI CONTENTS 4 CONVENTIONAL STAINING METHODS 195
GENERAL TISSUE STAINS 195 CELL WALL STAINS 197 CARBOHYDRATE AND STARCH
STAINS 200 LIPID STAINS 201 .__ NUCLEIC ACID STAINS 202 MISCELLANEOUS
STAINING METHODS 203 . REFERENCES 206 10 IMMUNOCYTOCHEMISTRY FOR LIGHT
MICROSCOPY 207 BEATRICE SATIAT-JEUNEMAITRE AND CHRIS HAWES 1
INTRODUCTION 207 2 PRINCIPLES AND USE OF IMMUNOCYTOCHEMISTRY 208 DIRECT
AND INDIRECT IMMUNOSTAINING 208 THE ANTIBODY-ANTIGEN COMPLEX 208 WHOLE
MOLECULES OR FRAGMENTS 209 POLYCLONAL AND MONOCLONAL ANTIBODIES 211 WHEN
TO PERFORM IN SITU IMMUNOREACTION 214 ANTIBODIES TO EPITOPE TAGS 215 3
BASIC METHODS FOR IMMUNOSTAINING 215 PREPARING PLANT MATERIAL 216
ATTACHING MATERIAL TO SLIDES AND COVERSLIPS 220 ACCESSING EPITOPES IN
CELLS 220 COUNTERSTAINING AND MOUNTING 230 INTERPRETING THE
IMMUNOSTAINING PATTERN 230 4 MULTIPLE STAINING 231 MULTIPLE
IMMUNOSTAINING 231 COMBINING IMMUNOSTAINING WITH OTHER AFFINITY
TECHNIQUES 231 5 CONCLUSION 232 REFERENCES 232 11 ELECTRON MICROSCOPY
235 CHRIS HAWES AND BEATRICE SATIAT-JEUNEMAITRE 1 INTRODUCTION 235 2
TRANSMISSION ELECTRON MICROSCOPY 235 CONVENTIONAL METHODS 235 LOW
TEMPERATURE METHODS 250 ROTARY SHADOWING OF PROTEINS 25 7 3 SCANNING
ELECTRON MICROSCOPY 258 AMBIENT TEMPERATURE SEM 258 LOW TEMPERATURE SEM
262 IMMUNO-SEM 263 ACKNOWLEDGEMENTS 263 REFERENCES 264 12 IN SITU
HYBRIDIZATION 267 A. R. LEITCH, K. Y. URN, D. R. WEBB, AND G. I.
MCFADDEN 1 INTRODUCTION 267 XLL CONTENTS 2 APPLICATIONS OF IN SITU
HYBRIDIZATION 269 DNA:DNA IN SITU HYBRIDIZATION 269 RNA:RNA IN SITU
HYBRIDIZATION 269 3 BACKGROUND TO THE METHODS 269 4 CHROMOSOME
PREPARATION FOR DNA:DNA IN SITU HYBRIDIZATION 270 ANIMAL MATERIAL 270
PLANT MATERIAL 271 5 MATERIAL PREPARATION FOR RNA:RNA IN SITU
HYBRIDIZATION 273 SPECIMEN PREPARATION 273 CONTROLS 275 6 LABELLING THE
NUCLEIC ACIDS 276 DNA LABELLING 276 CHECKING PROBE INCORPORATION 282 7
MATERIAL PRE-TREATMENT 283 PRE-TREATMENT FOR DNA:DNA IN SITU
HYBRIDIZATION 283 PRE-TREATMENT FOR RNA: RNA IN SITU HYBRIDIZATION 284 8
IN SITU HYBRIDIZATION REACTION 285 DNA:DNA IN SITU HYBRIDIZATION 285
RNA:RNA IN SITU HYBRIDIZATION 286 9 POST-HYBRIDIZATION WASHES 287 WASHES
FOR DNA:DNA IN SITU HYBRIDIZATION 288 WASHES FOR RNA:RNA IN SITU
HYBRIDIZATION 288 10 PROBE DETECTION AND VISUALIZATION 289 11
VISUALIZATION 290 EPIFLUORESCENCE MICROSCOPY 290 IMAGE CAPTURE 291 IMAGE
MANIPULATION 292 REFERENCES 292 13 ORGANELLE ISOLATION 295 DAVID G.
ROBINSON AND GISELBERT HINZ 1 INTRODUCTION 295 2 GENERAL METHODOLOGY 295
HOMOGENIZING MEDIA 295 METHODS OF HOMOGENIZATION 298 METHODS OF
ORGANELLE SEPARATION 299 3 ISOLATION OF CHLOROPLASTS 300 4 ISOLATION OF
MITOCHONDRIA 300 5 ISOLATION OF NUCLEI 301 6 ISOLATION OF MICROBODIES
303 7 ISOLATION OF PLASMA MEMBRANE 304 8 ISOLATION OF TONOPLAST 306 9
ISOLATION OF ENDOPLASMIC RETICULUM 308 10 ISOLATION OF GOLGI APPARATUS
310 11 ISOLATION OF TRANSPORT VESICLES 312 XLLL CONTENTS 12 ASSAYS FOR
MARKER ENZYMES 315 13 ANTIBODIES FOR ORGANELLE RECOGNITION 319
REFERENCES 320 AL LIST OF SUPPLIERS 325 INDEX 333 XIV PROTOCOL LIST
MICROSCOPE IMAGING MODES ADJUSTMENT FOR BRIGHT FIELD IMAGING WITH KOHLER
ILLUMINATION 11 ADJUSTMENT FOR PHASE CONTRAST 14 ADJUSTMENT FOR DIC 15
ADJUSTMENT FOR DARK FIELD 16 SPECIMEN PREPARATION FOR CONFOCAL IMAGING
30 COLLECTION OF CONFOCAL IMAGES 32 FLUORESCENT PROBES FOR LIVING PLANT
CELLS GROWTH OF ARABIDOPSIS THALIANA SEEDLINGS IN PHYTAGEL FOR IN SITU
OBSERVATION OF ROOTS 44 LOADING STRATEGIES FOR PLANT CELLS 46 LOADING
DYES BY VACUUM INFILTRATION OF LEAF PIECES 47 LOADING DYES AS
ACETOXYMETHYL ESTER OR ACETATE ESTER DERIVATIVES 47 LOW PH LOADING OF
ROOT TISSUES 49 INCREASING THE PERMEABILITY OF THE CUTICLE BY CUTINASE
DIGESTION 50 LOADING ROOT TISSUES WITH PHLOEM-MOBILE PROBES 51 DIRECT
OBSERVATION OF PHLOEM TRANSPORT IN BEAN LEAVES 52 SELECTION AND USE OF
FLUORESCENT PROBES 54 LABELLING OF ACTIN FILAMENTS IN LIVING CELLS USING
FLUORESCENT PHALLOTOXINS 63 LABELLING OF MICROTUBULES IN EPIDERMAL CELLS
USING FLUORESCENT ANALOGUE CYTOCHEMISTRY 64 PHYSIOLOGICAL PROBES 66 IN
VITRO CALIBRATION OF CALCIUM RATIO DYES 68 IN SITU CALIBRATION OF
CALCIUM DYES 71 IN SITU CALIBRATION OF RATIO PH DYES USING NIGERICIN 75
MEASUREMENT OF H 2 O 2 IN SITU USING H 2 DCF 77 DATA ANALYSIS 78
PROCESSING RATIO IMAGES 79 QUANTITATIVE ANALYSIS OF RATIO DATA FROM
REGIONS OF INTEREST 80 MEASUREMENT OF SIGNAL ATTENUATION WITH DEPTH
THROUGH A PERMEABILIZED SPECIMEN INFILTRATED WITH A FLUOROCHROME SEA
80 FLOW CYTOMETRY GENERAL TOOLKIT FOR ISOLATION OF DIVERSE NUCLEI FOR
CYTOMETRIC ANALYSIS 91 BRDU INCORPORATION TO IDENTIFY DNA SYNTHESIS BY
FLUORESCENCE QUENCHING 97 XV PROTOCOL LIST SORTING OF PROTOPLASTS AND
CELLULAR ORGANELLES SORTING SUBCLASSES OF PLANT NUCLEI 1 02 PRACTICAL
CONSIDERATIONS FOR CELL BIOLOGISTS PREPARATION OF ELECTROPORATION
COMPETENT PROTOPLASTS FROM TOBACCO LEAVES 113 ELECTROPORATION AND
SUBSEQUENT INCUBATION 115 HARVESTING CELLS AND MEDIUM FROM
ELECTROPORATED PROTOPLASTS 116 EXTRACTION AND CONCENTRATION OF PROTEINS
FROM CELLS AND MEDIUM 117 ISOLATION OF VACUOLES FROM PROTOPLASTS AFTER
SHORT TRANSIENT EXPRESSION 119 QUANTIFYING THE RECOVERY OF VACUOLES 120
CELL FRACTIONATION BY SUCROSE DENSITY CENTRIFUGATION 121 ASSESSMENT OF
MEMBRANE ASSOCIATION 123 ASSESSMENT OF MEMBRANE ORIENTATION 123
TRANSFORMATION METHODS PREPARATION OF PROTOPLASTS FROM STERILE TOBACCO
PLANTS 132 PREPARATION OF PROTOPLASTS FROM ANARABIDOPSIS CELL SUSPENSION
134 PEG-MEDIATED TRANSIENT EXPRESSION IN PROTOPLASTS 135
AGROBORTERIUM-MEDIATED TRANSIENT EXPRESSION IN TOBACCO LEAVES 136 PVX
VECTOR EXPRESSION IN WHOLE PLANTS 137 BIOLISTIC INOCULATION OF PVX
VECTOR CONSTRUCTS 138 MICROINJECTION EPIFLUORESCENCE OBSERVATION OF
PRESSURE INJECTION OF ALEXA 488 INTO EPIDERMAL CELLS 149 IONTOPHORETIC
MICROINJECTION OF LUCIFER YELLOW-CH POTASSIUM SALT (LYCH) INTO A
MESOPHYLL CELL 150 GEF-MEDIATED INJECTION OF 40 KDA DEXTRAN-LYCH
CONJUGATE INTO THE NUCLEUS OF A MOUGEOTIA CELL 152 GEF-MEDIATED
INJECTION OF LYCH IN A SINGLE CHLOROPLAST OF A GUARD CELL OF A YOUNG
TISSUE CULTURE LEAF 153 APPLICATIONS OF LASER MICROBEAMS IN PLANT
BIOLOGY INJECTION OF DNA INTO PLANT CELLS 164 APPLICATIONS OF OPTICAL
TWEEZERS TO PLANT BIOLOGY BASIC OPERATION OF A LASER TWEEZERS SET-UP 166
CAPACITANCE MEASUREMENTS AS AN ASSAY FOR EXO- AND ENDOCYTOSIS: PRACTICAL
CONSIDERATIONS MEASUREMENT OF SINGLE FUSION AND FISSION EVENTS IN THE
CELL-ATTACHED CONFIGURATION USING A DUAL-PHASE LOG-IN AMPLIFIER 1 77
MEASUREMENT OF SINGLE FUSION AND FISSION EVENTS IN THE WHOLE-CELL
CONFIGURATION USING A DUAL-PHASE LOG-IN AMPLIFIER 1 79 MONITORING OF
MACROSCOPIC CHANGES IN SURFACE AREA USING WHOLE-CELL MEMBRANE
CAPACITANCE MEASUREMENTS 181 COMBINED FLUORESCENT AND ELECTRICAL
MEASUREMENTS OF EXO- AND ENDOCYTOSIS 1 86 PLANT HISTOLOGY STANDARD
PARAFORMALDEHYDE-GLUTARALDEHYDE FIXATION 1 90 STANDARD FAA FIXATION 191
CONVENTIONAL EMBEDDING METHODS AND SCREENING 191 SILANIZED SLIDES 191
EMBEDDING IN AND SECTIONING POLYETHYLENE GLYCOL (PEG) 1 92 XVI PROTOCOL
LIST EMBEDDING IN AND SECTIONING WAX ^193 EMBEDDING IN AND SECTIONING LR
WHITE ACRYLIC RESIN 1 94 CONVENTIONAL STAINING METHODS 195 TOLUIDINE
BLUE 195 ACRIDINE ORANGE 196 HAEMATOXYLIN WITH COUNTERSTAINS 196
RUTHENIUM RED FOR PECTIN 198 CALCOFLUOR FOR CELLULOSE 198 PHLOROGLUCINOL
FOR LIGNIN 198 ANILINE BLUE FOR CALLOSE 199 RESORCINOL BLUE FOR CALLOSE
199 PAS FOR TOTAL CARBOHYDRATE 200 IODINE STAINING FOR STARCH 201 SUDAN
STAINING FOR LIPIDS 201 NILE BLUE FOR ACIDIC LIPIDS 202 METHYL
GREEN-PYRONIN FOR RNA AND DNA 202 FLUORESCENT STAINS FOR DNA 203 STAINS
FOR CELL VIABILITY 204 METHYL BLUE FOR FUNGI 204 THIONIN-ORANGE G FOR
FUNGI 204 GRAM STAIN FOR BACTERIA 205 PRINCIPLES AND USE OF
IMMUNOCYTOCHEMISTRY FRACTIONATIONOFLGGS FROM SERUM 211 TISSUE PRINTING
FOR OBSERVING IMMUNOLOGICAL AND PROTEIN PROFILES 216 GENERIC PROTOCOL
FOR IMMUNOFLUORESCENCE 218 IMMUNOFLUORESCENCE ON ROOT SQUASHES 220
IMMUNOFLUORESCENCE ON SUSPENSION CULTURE CELLS OR PROTOPLASTS 222
IMMUNOSTAINING OF POLLEN TUBES 223 GOLD LABELLING AND SILVER ENHANCEMENT
OF MUNG BEAN HYPOCOTYL MICROTUBULES (IGSS METHOD) 224 IMMUNOSTAINING ON
DE-WAXED SECTIONS 225 METHACRYLATE EMBEDDING, SECTIONING, AND
IMMUNOSTAINING 226 IMMUNOFLUORESCENCE ON CRYOSECTIONS OF ROOTS 228
IMMUNOSTAINING BY FREEZE-SHATTERING OF PLANT CELL WALLS 229 TRANSMISSION
ELECTRON MICROSCOPY PREPARATION OF A TYPICAL DOUBLE ALDEHYDE FIXATIVE
(1% PARAFORMALDEHYDE/ 2% GLUTARALDEHYDE) 237 FIXATION AND DEHYDRATION
237 RESIN EMBEDDING 240 PROGRESSIVE LOWERING OF TEMPERATURE AND ACRYLIC
RESIN EMBEDDING 242 PREPARATION OF REYNOLD S LEAD CITRATE 244 ZINC
IODIDE/OSMIUM TETROXIDE IMPREGNATION (ZIO) 245 PHOSPHOTUNGSTIC ACID
STAINING OF PLASMA MEMBRANES 245 PATAG STAINING OF CARBOHYDRATES 246 ON
GRID NEGATIVE STAINING 247 STANDARD IMMUNOGOLD LABELLING AND SILVER
ENHANCEMENT 249 TYPICAL FREEZE-SUBSTITUTION SCHEDULE 253 CRYOSECTIONING
AND IMMUNOGOLD LABELLING 254 XVN PROTOCOL LIST FREEZE-FRACTURE AND
REPLICATION INCLUDING DEEP-ETCHING AND ROTARY REPLICATION 256 ROTARY
METAL SHADOWING OF ISOLATED PROTEINS 258 BASIC PREPARATION OF MATERIAL
FOR AMBIENT TEMPERATURE SEM 259 CRITICAL-POINT DRYING 260 FRACTURING AND
OSMIUM MACERATION 261 CRYO-SEM 263 CHROMOSOME PREPARATION FOR DNA:DNA
IN SITU HYBRIDIZATION PREPARING MAMMAL CHROMOSOME SPREADS 270 PREPARING
PLANT CHROMOSOME SPREADS 272 MATERIAL PREPARATION FOR RNA:RNA IN SITU
HYBRIDIZATION 273 COATING OF SLIDES FOR CRYOSECTIONS 274 PREPARATION OF
CRYOSECTIONS 274 ACRIDINE ORANGE STAINING TO CHECK RETENTION OF NUCLEIC
ACIDS IN CRYOSECTIONS 275 LABELLING THE NUCLEIC ACIDS LABELLING OF DNA
BY NICK TRANSLATION 277 LABELLING DNA USING PCR 278 LABELLING RNA BY IN
VITRO TRANSCRIPTION 280 PRECIPITATION OF PROBE (FOR DNA OR RNA PROBES)
281 CHECKING LABEL INCORPORATION: DOT BLOT 282 MATERIAL PRE-TREATMENT
283 PRE-TREATMENT FOR DNA:DNA IN SITU HYBRIDIZATION PRE-TREATMENT FOR
RNA:RNA IN SITU HYBRIDIZATION: CRYOSECTIONS 284 IN SITU HYBRIDIZATION
REACTION DNA:DNA IN SITU HYBRIDIZATION REACTION 285 RNA:RNA IN SITU
HYBRIDIZATION 287 DNA:DNA POST-HYBRIDIZATION WASHES 288 RNA:RNA
POST-HYBRIDIZATION WASHES 288 PROBE DETECTION AND VISUALIZATION PROBE
DETECTION 290 ORGANELLE ISOLATION DETERGENT-ASSISTED HOMOGENIZATION OF
PROTOPLASTS 298 ISOLATION OF CHLOROPLASTS 300 ISOLATION OF INTACT
CHLOROPLASTS FROM SPINACH LEAVES 300 ISOLATION OF MITOCHONDRIA FROM
SPINACH LEAVES 301 ISOLATION OF NUCLEI FROM TOMATO LEAVES 303 ISOLATION
OF PEROXISOMES FROM SPINACH LEAVES 304 ISOLATION OF PLASMA MEMBRANES BY
AQUEOUS PHASE-PARTITIONING 305 LARGE SCALE PREPARATION OF VACUOLES FROM
STORAGE TISSUE 307 PREPARATION OF TONOPLAST MEMBRANES FROM ISOLATED
VACUOLES 308 ISOLATION OF ENDOPLASMIC RETICULUM 310 THE ISOLATION OF
INTACT DICTYOSOMES 312 ISOLATION OF CLATHRIN-COATED VESICLES 313 THE
ISOLATION OF SECRETORY VESICLES FROM POLLEN TUBES 314 MARKER ENZYMES 316
XV111
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| any_adam_object | 1 |
| building | Verbundindex |
| bvnumber | BV013662482 |
| callnumber-first | Q - Science |
| callnumber-label | QK673 |
| callnumber-raw | QK673 |
| callnumber-search | QK673 |
| callnumber-sort | QK 3673 |
| callnumber-subject | QK - Botany |
| classification_rvk | WC 5100 WC 5102 WN 1300 |
| ctrlnum | (OCoLC)614120830 (DE-599)BVBBV013662482 |
| discipline | Biologie |
| edition | 2. ed. |
| format | Book |
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| id | DE-604.BV013662482 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:49:50Z |
| institution | BVB |
| isbn | 0199638667 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009336243 |
| oclc_num | 614120830 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-29T DE-20 DE-634 DE-11 |
| owner_facet | DE-355 DE-BY-UBR DE-29T DE-20 DE-634 DE-11 |
| physical | XX, 338 S. Ill., graph. Darst. |
| publishDate | 2001 |
| publishDateSearch | 2001 |
| publishDateSort | 2001 |
| publisher | Oxford Univ. Press |
| record_format | marc |
| series | The practical approach series |
| series2 | The practical approach series |
| spelling | Plant cell biology ed. by Chris Hawes ... 2. ed. Oxford Oxford Univ. Press 2001 XX, 338 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier The practical approach series 250 Cytologie (DE-588)4070177-3 gnd rswk-swf Cytochemie (DE-588)4070172-4 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Pflanzenzelle (DE-588)4115551-8 gnd rswk-swf Pflanzenzelle (DE-588)4115551-8 s Cytochemie (DE-588)4070172-4 s DE-604 Cytologie (DE-588)4070177-3 s Methode (DE-588)4038971-6 s Hawes, Chris Sonstige oth The practical approach series 250 (DE-604)BV007921321 250 HEBIS Datenaustausch Darmstadt application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009336243&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Plant cell biology The practical approach series Cytologie (DE-588)4070177-3 gnd Cytochemie (DE-588)4070172-4 gnd Methode (DE-588)4038971-6 gnd Pflanzenzelle (DE-588)4115551-8 gnd |
| subject_GND | (DE-588)4070177-3 (DE-588)4070172-4 (DE-588)4038971-6 (DE-588)4115551-8 |
| title | Plant cell biology |
| title_auth | Plant cell biology |
| title_exact_search | Plant cell biology |
| title_full | Plant cell biology ed. by Chris Hawes ... |
| title_fullStr | Plant cell biology ed. by Chris Hawes ... |
| title_full_unstemmed | Plant cell biology ed. by Chris Hawes ... |
| title_short | Plant cell biology |
| title_sort | plant cell biology |
| topic | Cytologie (DE-588)4070177-3 gnd Cytochemie (DE-588)4070172-4 gnd Methode (DE-588)4038971-6 gnd Pflanzenzelle (DE-588)4115551-8 gnd |
| topic_facet | Cytologie Cytochemie Methode Pflanzenzelle |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009336243&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV007921321 |
| work_keys_str_mv | AT haweschris plantcellbiology |