Cytometric analysis of cell phenotype and function:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Cambridge [u.a.]
Cambridge Univ. Press
2001
|
| Ausgabe: | 1. publ. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 413 S. graph. Darst. |
| ISBN: | 0521660297 |
Internformat
MARC
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| 245 | 1 | 0 | |a Cytometric analysis of cell phenotype and function |c ed. by Desmond A. McCarthy ... |
| 250 | |a 1. publ. | ||
| 264 | 1 | |a Cambridge [u.a.] |b Cambridge Univ. Press |c 2001 | |
| 300 | |a XVI, 413 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 4 | |a Cytologie - Guides, manuels, etc | |
| 650 | 7 | |a Cytometrie |2 gtt | |
| 650 | 4 | |a Cytométrie de flux - Guides, manuels, etc | |
| 650 | 4 | |a Cell Physiological Phenomena | |
| 650 | 4 | |a Cytology |v Handbooks, manuals, etc | |
| 650 | 4 | |a Flow Cytometry |x methods | |
| 650 | 4 | |a Flow cytometry |v Handbooks, manuals, etc | |
| 650 | 4 | |a Phenotype | |
| 650 | 0 | 7 | |a Cytometrie |0 (DE-588)4148710-2 |2 gnd |9 rswk-swf |
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| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a MacCarthy, Desmond A. |e Sonstige |4 oth | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-009335093 | ||
Datensatz im Suchindex
| _version_ | 1804128473673891840 |
|---|---|
| adam_text | Contents
List of contributors xi
List of abbreviations xiii
1 Principles of flow cytometry 1
Marion G. Macey
1.1 History and development of flow cytometry 1
1.2 Principles of flow cytometry 1
1.3 Fluorescence analysis 4
1.4 Light scatter and fluorescence detection 5
1.5 Acquisition 7
1.6 Amplification 8
1.7 Histograms 8
1.8 Coefficients of variation 9
1.9 Spectral overlap and compensation 10
1.10 Safety aspects of lasers 10
1.11 Cell sorting 11
1.12 Commercial flow cytometers 13
1.13 References 13
2 Introduction to the general principles of
sample preparation 15
Desmond A. McCarthy
2.1 Introduction 15
2.2 Factors affecting the choice of preparation
procedure 15
2.3 Blood and bone marrow 16
2.4 Preparation of cell suspensions from
solid tissues and cell cultures 26
2.5 Live samples 29
2.6 Fixed samples 30
V
Contents
2.7 Permeabilisation techniques for
immunolabelling intracellular components 32
2.8 Immunolabelling 33
2.9 Determining absolute cell counts 41
2.10 Safety 41
2.11 Further reading 41
2.12 Internet resources 42
2.13 References 42
3 Fluorescence and fluorochromes 45
Desmond A. McCarthy
3.1 Introduction 45
3.2 The interaction of light and matter 45
3.3 Light absorption and fluorescence 46
3.4 Energy transfer by resonance between
fluorochromes 50
3.5 Fluorochrome options for cytometric
applications 52
3.6 The characteristics of fluorochromes used for
conjugating to antibodies and other proteins 57
3.7 Coupling fluorochromes to antibodies or other
proteins 60
3.8 Linking antibodies to fluorochromes through
protein A or G, avidin or streptavidin biotin
links 61
3.9 Fluorescent dyes for labelling nucleic acids 62
3.10 Probes for cell viability and death: necrosis and
apoptosis 64
3.11 Fluorescent dyes for the detection of
intracellular ions 67
3.12 Probes for phagocytosis and oxidative
metabolism 69
3.13 Fluorochrome labelled substrate analogues for
measuring enzyme activity 70
3.14 Fluorescent ligands 71
3.15 Fluorescent dyes for measuring total protein 71
3.16 Autofluorescence 71
3.17 Further reading 72
3.18 Internet sites 72
3.19 References 72
4 Quality control in flow cytometry 74
David Barnett and John T. Reilly
4.1 Introduction 74
4.2 Internal quality control 74
4.3 Instrument quality control 74
4.4 Quality control issues and pitfalls 77
4.5 External quality assessment 81
4.6 Reagent selection 82
4.7 Definition of positive values 83
4.8 Absolute count enumeration 84
4.9 Conclusion 84
4.10 References 85
5 Data analysis in flow cytometry 89
MarkW. Lowdell
5.1 Introduction 89
5.2 Experiment design 89
5.3 Analysis of flow cytometric cell phenotyping
data 89
5.4 Cluster analysis 90
5.5 Measurement of fluorescent intensity 92
5.6 Standardisation 95
5.7 Logarithmic and linear amplification 96
5.8 Fluorescence calibration and quantification 96
5.9 Conclusions 99
5.10 References 99
6 Laser scanning cytometry: application
to the immunophenotyping of
hematological malignancies 100
Richard J.Clatch
6.1 General considerations for the
immunophenotyping of leukaemias and
lymphomas 100
6.2 The laser scanning cytometer 102
6.3 Immunophenotyping by laser scanning
cytometry 104
6.4 Extensions to the basic method 110
6.5 Conclusions 115
6.6 Internet sites 116
6.7 References 116
7 Leukocyte immunobiology 118
Marion G. Macey
7.1 Introduction to human leukocytes and
leukocyte antigens 118
7.2 Monoclonal antibodies and clusters of
differentiation 118
7.3 Surface antigen changes during hematopoiesis 118
7.4 Progenitor cell differentiation 119
7.5 Myeloid maturation 120
7.6 B lymphocyte maturation 121
7.7 T lymphocyte maturation 122
7.8 Natural killer cell maturation 124
7.9 Dendritic cell maturation 125
7.10 Peripheral blood lymphocyte subpopulations:
normal ranges 126
7.11 The identification of leukocyte subclasses by
flow cytometry 128
7.12 Analysis of surface and intracellular molecules 131
7.13 Characteristic changes in lymphocyte subsets
in disease 131
7.14 The identification and quantification of
lymphocyte functional subsets 131
7.15 Measurement of leukocyte activation 131
7.16 Human hemopoietic cell lines 133
7.17 References 133
8 Immunophenotypic analysis of
leukocytes in disease 138
Jamie D. Cavenagh, Marion G. Macey
and Timothy M. Milne
8.1 Introduction 138
8.2 Classification of leukaemias 139
8.3 Acute myeloid leukaemia 140
8.4 The classification of B cell acute lymphoblastic
leukaemia 142
8.5 T lineage acute lymphoblastic leukaemia 143
8.6 Biphenotypic and undifferentiated acute
leukaemia 144
8.7 B cell lymphomas and chronic leukaemias 145
8.8 Chronic T cell leukaemias 151
8.9 Myeloproliferative disorders 152
8.10 Flow cytometric analysis of leukaemic
samples 152
8.11 Therapeutic monitoring 152
Contents
( 8.12 Diagnosis of other hematological disorders 157
8.13 Analysis of genetic variation in polymorphic
markers 159
8.14 Transplantation crossmatching 159
8.15 References 160
9 Analysis and isolation of minor cell
populations 165
Terry Hoy
9.1 Introduction 165
9.2 Application of statistical equations to
conventional situations 166
9.3 Application of statistical equations to small
subpopulations (rare events) 166
9.4 Practical examples illustrating different
features of rare event analysis 167
9.5 The statistics of sorting using a flow
cytometer 174
9.6 Conclusions 179
9.7 References 180
10 Cell cycle, DNA and ONA ploidy analysis 182
Paul D. Allen and Adrian C. Newland
10.1 Introduction 182
10.2 Cell cycling 182
10.3 Cell cycle regulation 183
10.4 Flow cytometric analysis of the cell cycle 186
10.5 DNA content to determine cell cycle
distribution 187
10.6 S phase analysis using bromodeoxyuridine
uptake 188
10.7 Numerical chromosomal aberrations 189
10.8 Conclusions 19]
10.9 References 192
11 Cell viability, necrosis and apoptosis 193
Paul D. Allen and Adrian C. Newland
11.1 Introduction 193
11.2 Apoptosis 193
11.3 Genetic control of apoptosis 193
11.4 Bcl 2 expression and apoptosis 194
11.5 Necrosis 194
Contents
11.6 Mitochondrial membrane potential,
cytochrome c and cell death 195
11.7 Flow cytometry 196
11.8 Protocols 203
11.9 References 203
12 Phagocyte biology and function 204
Marion G. Macey
12.1 Introduction to phagocyte biology 204
12.2 Maturational states of neutrophils and
monocytes 205
12.3 Expression of functional antigens and
receptors on the cell surface 206
12.4 Receptor signalling 207
12.5 Priming and activation 209
12.6 Prolonged responses to cytokines and/or
hormones 210
12.7 Chemoattractant binding and rapid responses
to chemotaxins/activators 211
12.8 Shape changes 211
12.9 Membrane potential and changes in ion
permeability 213
12.10 Phagocytosis, endocytosis and oxidative burst 214
12.11 Nitric oxide release 218
12.12 Multiparameter techniques to assess function
andphenotype 218
12.13 Clinical applications of phagocyte function
assays 218
12.14 Neutrophil antibodies 220
12.15 Clinical applications of flow cytometry to the
study of autoimmune neutropenia 224
12.16 Neutrophil IgG Fc receptor expression and
function 224
12.17 Conclusions 225
12.18 References 225
13 Intracellular measures of signalling
pathways 231
James W. Jacobberger and David W. Hedley
13.1 Introduction 231
13.2 Regulatory pathways 232
13.3 Detection of intracellular protein epitopes 233
13.4 Determination of DNA and protein content 237
13.5 Assessing the physiological status of cells 237
13.6 Monitoring physiological changes during cell
death 238
13.7 Immunodetection of target molecules and
pathways 242
13.8 Protocols 244
13.9 Authors comments 244
13.10 References 244
14 Cell cell interactions 247
Marion G. Macey and Mary R. Cahill
14.1 Adhesion molecules in cell cell interactions 247
14.2 Analysis of cell cell interactions 251
14.3 Platelet platelet interactions 253
14.4 Platelet activation in clinical disorders 254
14.5 Flow cytometric analysis of platelet activation 255
14.6 Methods for the analysis of platelet adhesion
and activation molecules 256
14.7 Platelet leukocyte interactions 258
14.8 Leukocyte leukocyte interactions 258
14.9 Leukocyte endothelial cell interactions 260
14.10 Dysregulated cell cell interactions and
adhesion molecule deficiency states 261
14.11 Defects in human integrins 261
14.12 Defects in other human adhesion molecules 262
14.13 Pharmacologically induced deficiency states 263
14.14 Analysis of platelet directed antibodies 263
14.15 Fluorescence resonance energy transfer 265
14.16 Clinical relevance of platelet directed
antibodies 266
14.17 References 266
15 Nucleic acids 271
Charles L. Goolsby
15.1 Introduction 271
15.2 Flowkaryotyping 273
15.3 Fluorescence in situ hybridisation analyses
with cells in suspension 277
15.4 Approaches using in situ polymerase
chain reaction or reverse transcriptase polymerase
chain reaction with cells in suspension 283
15.5 Conclusions 288
15.6 References 288
16 Microbial infections 292
David Lloyd, David J. Mason
and Marc T.E. Suller
16.1 Introduction 292
16.2 Viruses 292
16.3 Bacteria 296
16.4 Yeasts 302
16.5 Protozoa 304
16.6 The immune response to infection 306
16.7 Conclusions 308
16.8 Internet sites 309
16.9 References 309
17 Leucocyte cell surface antigens 315
Marion G. Macey
17.1 Introduction 315
17.2 Modules and domains 315
17.3 Clusters of differentiation 317
17.4 References 366
Contents
18 Recent and future developments:
conclusions 378
Desmond A. McCarthy
18.1 Introduction 378
18.2 Developments in fluorescent labels 378
18.3 Gaining additional information from light
scatter and fluorescence signals 381
18.4 Portable flow cytometers 384
18.5 Microscale analysis and sorting using
miniature fluidic circuits 384
18.6 New static cytometers 385
18.7 New applications of existing technologies 386
18.8 Current and future clinical needs 389
18.9 Conclusions 393
18.10 Internet sites 393
18.11 References 394
Appendix 396
Useful Internet sites 396
A. 1 Sites of general interest for cytometry 396
A.2 Suppliers of instruments, reagents,
antibodies etc. 397
Index 403
Colour plates between pages 112 and 113
|
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| indexdate | 2024-07-09T18:49:48Z |
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| isbn | 0521660297 |
| language | English |
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| physical | XVI, 413 S. graph. Darst. |
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| spelling | Cytometric analysis of cell phenotype and function ed. by Desmond A. McCarthy ... 1. publ. Cambridge [u.a.] Cambridge Univ. Press 2001 XVI, 413 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Cytologie - Guides, manuels, etc Cytometrie gtt Cytométrie de flux - Guides, manuels, etc Cell Physiological Phenomena Cytology Handbooks, manuals, etc Flow Cytometry methods Flow cytometry Handbooks, manuals, etc Phenotype Cytometrie (DE-588)4148710-2 gnd rswk-swf Cytometrie (DE-588)4148710-2 s DE-604 MacCarthy, Desmond A. Sonstige oth HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009335093&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cytometric analysis of cell phenotype and function Cytologie - Guides, manuels, etc Cytometrie gtt Cytométrie de flux - Guides, manuels, etc Cell Physiological Phenomena Cytology Handbooks, manuals, etc Flow Cytometry methods Flow cytometry Handbooks, manuals, etc Phenotype Cytometrie (DE-588)4148710-2 gnd |
| subject_GND | (DE-588)4148710-2 |
| title | Cytometric analysis of cell phenotype and function |
| title_auth | Cytometric analysis of cell phenotype and function |
| title_exact_search | Cytometric analysis of cell phenotype and function |
| title_full | Cytometric analysis of cell phenotype and function ed. by Desmond A. McCarthy ... |
| title_fullStr | Cytometric analysis of cell phenotype and function ed. by Desmond A. McCarthy ... |
| title_full_unstemmed | Cytometric analysis of cell phenotype and function ed. by Desmond A. McCarthy ... |
| title_short | Cytometric analysis of cell phenotype and function |
| title_sort | cytometric analysis of cell phenotype and function |
| topic | Cytologie - Guides, manuels, etc Cytometrie gtt Cytométrie de flux - Guides, manuels, etc Cell Physiological Phenomena Cytology Handbooks, manuals, etc Flow Cytometry methods Flow cytometry Handbooks, manuals, etc Phenotype Cytometrie (DE-588)4148710-2 gnd |
| topic_facet | Cytologie - Guides, manuels, etc Cytometrie Cytométrie de flux - Guides, manuels, etc Cell Physiological Phenomena Cytology Handbooks, manuals, etc Flow Cytometry methods Flow cytometry Handbooks, manuals, etc Phenotype |
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