Fluorescence kinetics of the green fluorescent protein (GFP):
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2000
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 51 S. Ill., graph. Darst. |
Internformat
MARC
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| 100 | 1 | |a Kummer, Andreas |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Fluorescence kinetics of the green fluorescent protein (GFP) |c Andreas Kummer |
| 264 | 1 | |c 2000 | |
| 300 | |a 51 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a München, Techn. Univ., Diss. | ||
| 650 | 7 | |a Hochschulschrift |2 gtt | |
| 650 | 0 | 7 | |a Absorptionsspektroskopie |0 (DE-588)4141129-8 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Grün fluoreszierendes Protein |0 (DE-588)4508128-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Fluoreszenzspektroskopie |0 (DE-588)4017701-4 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Grün fluoreszierendes Protein |0 (DE-588)4508128-1 |D s |
| 689 | 0 | 1 | |a Absorptionsspektroskopie |0 (DE-588)4141129-8 |D s |
| 689 | 0 | 2 | |a Fluoreszenzspektroskopie |0 (DE-588)4017701-4 |D s |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009309279 | |
Datensatz im Suchindex
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| adam_text |
TABLE OF CONTENTS
3
1. INTRODUCTION.5
1.1 DISCOVERY AND MAJOR MILESTONES.5
1.2 PRIMARY, SECONDARY AND TERTIARY STRUCTURE.5
2. EXPERIMENTAL METHODS.9
2.1 STEADY-STATE ABSORPTION AND FLUORESCENCE MEASUREMENTS.9
2.2 PICOSECOND TIME-RESOLVED FLUORESCENCE MEASUREMENTS.9
2.2.1 TIME-CORRELATED SINGLE-PHOTON COUNTING.
V.10
2.2.2 THE STREAK CAMERA METHOD.10
2.2.3 THE NUMERICAL ANALYSIS OF THE MEASUREMENTS.11
2.3 TIME-RESOLVED ABSORPTION MEASUREMENTS.12
2.3.1 ABSORPTION MEASUREMENT WITH 100 PS TIME RESOLUTION.12
2.3.2 FEMTOSECOND TIME-RESOLVED ABSORPTION MEASUREMENTS.12
2.4 THE SAMPLES.12
2.4.1 WILD-TYPE GFP, RSGFP8, BFP11, RS8/T203Y AND RS8/ORG18.12
2.4.2 WILD-TYPE GFP, PH 7.4 AND SINGLE-SITE MUTANTS.13
3. EXCITED STATE DEPROTONATION.15
3.1 MOTIVATION.15
3.2 RESULTS.15
3.2.1 STEADY-STATE ABSORPTION AND FLUORESCENCE SPECTRA.15
3.2.1.1 WILD-TYPE GFP IN AQUEOUS BUFFER - ROOM TEMPERATURE.15
3.2.1.2 EFFECT OF CRYOPROTECTORS ON ABSORPTION SPECTRA.16
3.2.1.3 WILD-TYPE GFP IN 50% GLYCEROL - LOW-TEMPERATURE SPECTRA.16
3.2.2 PICOSECOND TIME-RESOLVED FLUORESCENCE STUDIES.17
3.2.2.1 WILD-TYPE GFP.17
3
.
2.22 MUTANTS.19
3.2.2.3 BLUE-SHIFTED MUTANT BFP11.19
3.2.2.4 RED-SHIFTED MUTANT RSGFP8.19
3.2.3 TIME-RESOLVED ABSORPTION MEASUREMENTS.20
3.2.3.1 PICOSECOND TRANSIENT SPECTROSCOPY.20
3.2.3.2 FEMTOSECOND TRANSIENT SPECTROSCOPY.20
3.2.3.3 WILD-TYPE GFP.20
3.2.3.4 RED-SHIFTED MUTANT RSGFP8. 20
3.3 DISCUSSION.I.21
3.3.1 MECHANISM OF GFP EMISSION.21
3.3.2 GROUND STATE EQUILIBRIUM BETWEEN PROTONATED AND DEPROTONATED
SPECIES - EFFECT OF CRYOPROTECTORS.22
3.3.3 ABSORPTION, FLUORESCENCE AND FLUORESCENCE EXCITATION SPECTRA AT
HIGH AND LOW TEMPERATURE.22
3.3.4 ACCUMULATIVE SUPPORT FOR EXCITED STATE PROTON TRANSFER
MECHANISM.23
3.3.4.1 THE PICOSECOND DECAY OF RH* DETECTED AT 450 NM IN FLUORESCENCE
AND THE CONCOMITANT RISE CHARACTERISTICS OF THE FLUORESCENCE FROM
R~NEQ* AT 510 NM.23
3.3.4.2 STRONG DEUTERATION EFFECT ON THE CORRELATED KINETICS OF
FLUORESCENCE DECAY FROM RH(D)* AND FORMATION OF R"^.24
3.3.4.3 FAST REPROTONATION.24
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/961142227
4
3.3.4.4 MUTANTS.24
3.4 REMARKS.25
3.4.1 EXCITED STATE PROTON TRANSFER AT LOW TEMPERATURE.25
3.4.2 DISPERSIVE KINETICS.25
4. FAST INTERNAL CONVERSION IN MUTANTS OF GFP.27
4.1 MOTIVATION.27
4.2 RESULTS.27
4.2.1 COMPARISON OF STEADY-STATE ABSORPTION, FLUORESCENCE EMISSION AND
EXCITATION SPECTRA OF MUTANTS RS8/T203Y AND RS8/ORG18 WITH THEIR
PARENT MUTANT RSGFP8 AND WILD-TYPE GFP.27
4.2.2 PICOSECOND TIME-RESOLVED FLUORESCENCE SPECTROSCOPY.28
4.2.3 FEMTOSECOND TRANSIENT ABSORPTION MEASUREMENTS.29
4.3 DISCUSSION.30
4.3.1 FAST INTERNAL CONVERSION IN THE MUTANT RS8/T203Y UPON EXCITATION
OF
RH AND IN THE MUTANT RS8/ORG18 UPON EXCITATION OF R~.30
4.3.2 COMPARISON WITH WILD-TYPE GFP.'.31
4.3.3 EFFECTS OF GROUND STATE EQUILIBRIUM BETWEEN R" AND RH.31
5. EFFECTS OF THREONINE 203 REPLACEMENTS.33
5.1 MOTIVATION.33
5.2 RESULTS.35
5.2.1 COMPARISON OF STEADY-STATE ABSORPTION, FLUORESCENCE EMISSION AND
EXCITATION SPECTRA OF THE T203 MUTANTS WITH WILD-TYPE GFP.35
5.2.2 PICOSECOND TIME-RESOLVED FLUORESCENCE MEASUREMENTS.37
5.2.3 FEMTOSECOND TRANSIENT ABSORPTION MEASUREMENTS.37
5.3 DISCUSSION.38
6. THE SINGLE-SITE BLUE EMISSION VARIANTS Y66F AND Y66H.41
6.1 MOTIVATION.!.41
6.2 RESULTS AND DISCUSSION.41
6.2.1 THE MUTANTS Y66F AND Y66H. STEADY-STATE ABSORPTION AND
FLUORESCENCE.41
6.2.2 THE MUTANT Y66F. TIME-RESOLVED FLUORESCENCE.41
6.2.3 THE MUTANT Y66H.42
7. SUMMARY.45
A. REFERENCES.47
B. PUBLICATIONS
51 |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV013626871 |
| illustrated | Illustrated |
| indexdate | 2024-10-09T18:09:31Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009309279 |
| oclc_num | 48729006 |
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| owner | DE-91 DE-BY-TUM DE-12 DE-29T DE-188 |
| owner_facet | DE-91 DE-BY-TUM DE-12 DE-29T DE-188 |
| physical | 51 S. Ill., graph. Darst. |
| publishDate | 2000 |
| publishDateSearch | 2000 |
| publishDateSort | 2000 |
| record_format | marc |
| spelling | Kummer, Andreas Verfasser aut Fluorescence kinetics of the green fluorescent protein (GFP) Andreas Kummer 2000 51 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Techn. Univ., Diss. Hochschulschrift gtt Absorptionsspektroskopie (DE-588)4141129-8 gnd rswk-swf Grün fluoreszierendes Protein (DE-588)4508128-1 gnd rswk-swf Fluoreszenzspektroskopie (DE-588)4017701-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Grün fluoreszierendes Protein (DE-588)4508128-1 s Absorptionsspektroskopie (DE-588)4141129-8 s Fluoreszenzspektroskopie (DE-588)4017701-4 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009309279&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kummer, Andreas Fluorescence kinetics of the green fluorescent protein (GFP) Hochschulschrift gtt Absorptionsspektroskopie (DE-588)4141129-8 gnd Grün fluoreszierendes Protein (DE-588)4508128-1 gnd Fluoreszenzspektroskopie (DE-588)4017701-4 gnd |
| subject_GND | (DE-588)4141129-8 (DE-588)4508128-1 (DE-588)4017701-4 (DE-588)4113937-9 |
| title | Fluorescence kinetics of the green fluorescent protein (GFP) |
| title_auth | Fluorescence kinetics of the green fluorescent protein (GFP) |
| title_exact_search | Fluorescence kinetics of the green fluorescent protein (GFP) |
| title_full | Fluorescence kinetics of the green fluorescent protein (GFP) Andreas Kummer |
| title_fullStr | Fluorescence kinetics of the green fluorescent protein (GFP) Andreas Kummer |
| title_full_unstemmed | Fluorescence kinetics of the green fluorescent protein (GFP) Andreas Kummer |
| title_short | Fluorescence kinetics of the green fluorescent protein (GFP) |
| title_sort | fluorescence kinetics of the green fluorescent protein gfp |
| topic | Hochschulschrift gtt Absorptionsspektroskopie (DE-588)4141129-8 gnd Grün fluoreszierendes Protein (DE-588)4508128-1 gnd Fluoreszenzspektroskopie (DE-588)4017701-4 gnd |
| topic_facet | Hochschulschrift Absorptionsspektroskopie Grün fluoreszierendes Protein Fluoreszenzspektroskopie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009309279&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT kummerandreas fluorescencekineticsofthegreenfluorescentproteingfp |