DNA topoisomerase protocols: 2 Enzymology and drugs
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2001
|
| Series: | Methods in molecular biology
95 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XV, 333 S. Ill., graph. Darst. |
| ISBN: | 0896035123 |
Staff View
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Record in the Search Index
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| adam_text | Contents
Preface v
Contributors xi
Contents of Companion Volume xv
1 Assaying DNA Topoisomerase I Relaxation Activity
Lance Stewart and James J. Champoux 7
2 DNA Topoisomerase Il Catalyzed DNA Decatenation
Andrea Haldane and Daniel M. Sullivan 13
3 Plasmid DNA Supercoiling by DNA Gyrase
Penny J. Sayer, Martin L. Goble, Mark Oram,
and L. Mark Fisher 25
4 Analyzing Reverse Gyrase Activity
Michel Duguet, Christine Jaxel, Anne Cecile Declais,
Fabrice Confalonieri, Janine Marsault,
Claire Bouthier de la Tour, Marc Nadal,
and Christiane Portemer 35
5 Topoisomerase Il Catalyzed ATP Hydrolosis as Monitored
by Thin Layer Chromatography
Paul S. Kingma, John M. Fortune, and Neil Osheroff 51
6 Use of a Real Time, Coupled Assay to Measure the ATPase
Activity of DNA Topoisomerase II
Janet E. Lindsley 57
7 Analysis of Topoisomerase DNA Interactions
by Electrophoretic Mobility Shift Assay
Stewart Shuman 65
8 Filter Binding Assays for Topoisomerase DNA Complexes
Joaquim Roca 75
9 DNA Topoisomerase I Mediated Nicking of Circular Duplex DNA
James J. Champoux 81
10 Analysis of Cleavage Complexes Using Reactive Inhibitor Derivatives
Kenneth N. Kreuzer, Catherine H. Freudenreich,
and Yves Pommier 89
vii
viii Contents
11 Uncoupling of Topoisomerase Mediated
DNA Cleavage and Religation
Anni H. Anderson, Kent Christiansen,
and Ole Westergaard 101
12 Synthesis and Use of DNA Containing a 5 Bridging Phosphorothioate
as a Suicide Substrate for Type I DNA Topoisomerases
Alex B. Burgin, Jr. 119
13 Isolation of Covalent Enzyme DNA Complexes
Thomas C. Rowe, Dale Grabowski, and Ram Ganapathi 129
14 ICE Bioassay: Isolating In Vivo Complexes of Enzyme to DNA
Deepa Subramanian, Christine Sommer Furbee,
and Mark T. Muller 137
15 DNA Unwinding Test Using Eukaryotic DNA Topoisomerase I
Linus L. Shen 149
16 Drug DNA Interactions
David E. Graves 161
17 Quinolone Interactions with DNA and DNA Gyrase
Linus L. Shen 171
18 Bactericidal Assays for Fluoroquinolones
Thomas D. Gootz 185
19 Drug Toxicity in E. co//Cells Expressing
Human Topoisomerase I
Scott T. Taylor and Rolf Menzel 195
20 Drug Induced Cytotoxicity in Tissue Culture
Cynthia E. Herzog and Leonard A. Zwelling 205
21 Analysis of Drug Uptake Flow Cytometry
Grace Zoorob and Thomas Burke 215
22 Cell Cycle Analysis of Drug Treated Cells
Frank Traganos, Gloria Juan, and Zbigniew Darzynkiewicz 229
23 Assaying Drug Induced Apoptosis
Zbigniew Darzynkiewicz, Gloria Juan, and Frank Traganos 241
24 Single Strand Conformational Polymorphism Analysis
of DNA Topoisomerases I and II
Mary K. Danks 255
25 PCR Based Cloning of DNA Topoisomerase Genes
Wai Mun Huang 265
26 Topoisomerase Il Catalyzed Relaxation and Catenation
of Plasmid DNA
John M. Fortune and Neil Osheroff 275
Contents ix
27 Topoisomerase Il Mediated Cleavage of Plasmid DNA
D. Andrew Burden, Stacie J. Froelich Ammon,
and Neil Osheroff 283
28 Drug induced Stabilization of Covalent DNA Topoisomerase
I DNA Intermediates: DNA Cleavage Assays
Paola Fiorani, Christine L. Hann, Piero Benedetti,
and Mary Ann Bjornsti 291
29 Studying DNA Topoisomerase I Targeted Drugs
in the Yeast: Saccharomyces cerevisiae
Michael H. Woo, John R. Vance, and Mary Ann Bjornsti 303
30 Yeast Systems for Demonstrating the Targets
of Anti Topoisomerase II Agents
John L. Nitiss and Karin C. Nitiss 315
Index 329
|
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| indexdate | 2024-07-09T18:47:28Z |
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| isbn | 0896035123 |
| language | English |
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| physical | XV, 333 S. Ill., graph. Darst. |
| publishDate | 2001 |
| publishDateSearch | 2001 |
| publishDateSort | 2001 |
| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | DNA topoisomerase protocols 2 Enzymology and drugs ed. by Neil Osheroff ... Totowa, NJ Humana Press 2001 XV, 333 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 95 Methods in molecular biology ... Methode (DE-588)4038971-6 gnd rswk-swf DNS-Gyrase (DE-588)4285125-7 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf DNS-Gyrase (DE-588)4285125-7 s Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s DE-604 Bjornsti, Mary-Ann Sonstige oth Osheroff, Neil Sonstige oth (DE-604)BV012594847 2 Methods in molecular biology 95 (DE-604)BV035362695 95 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009237399&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | DNA topoisomerase protocols Methods in molecular biology Methode (DE-588)4038971-6 gnd DNS-Gyrase (DE-588)4285125-7 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4285125-7 (DE-588)4039983-7 |
| title | DNA topoisomerase protocols |
| title_auth | DNA topoisomerase protocols |
| title_exact_search | DNA topoisomerase protocols |
| title_full | DNA topoisomerase protocols 2 Enzymology and drugs ed. by Neil Osheroff ... |
| title_fullStr | DNA topoisomerase protocols 2 Enzymology and drugs ed. by Neil Osheroff ... |
| title_full_unstemmed | DNA topoisomerase protocols 2 Enzymology and drugs ed. by Neil Osheroff ... |
| title_short | DNA topoisomerase protocols |
| title_sort | dna topoisomerase protocols enzymology and drugs |
| topic | Methode (DE-588)4038971-6 gnd DNS-Gyrase (DE-588)4285125-7 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| topic_facet | Methode DNS-Gyrase Molekularbiologie |
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