Development and application of PCR-based diagnostic assays for toxigenic fungi in cereals and malts:
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| Main Author: | |
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| Format: | Book |
| Language: | English |
| Published: |
Stuttgart
Grauer
2000
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Zugl.: München, Techn. Univ., Diss., 2000 |
| Physical Description: | 126 S. Ill., graph. Darst. |
| ISBN: | 3861863235 |
Staff View
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Record in the Search Index
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| adam_text | Titel: Development and application of PCR-based diagnostic assays for toxigenic fungi in cereals and malts
Autor: Mulfinger, Sven
Jahr: 2000
Content
1. Introduction ............................................................................................................... 8
1.1 Fungi on cereals ................................................................................................. 8
1.2 Fusarium head blight in cereals ......................................................................... 8
1.2.1 Mycotoxicosis ......................................................................................... 13
1.3 Fusarium mycotoxins ........................................................................................ 14
1.3.1 Type B Trichothecenes .......................................................................... 21
Zearalenone
Deoxynivalenol
Nivalenol
Fusarenone X
1.3.2 Other Fusarium toxins ........................................................................... 23
Fumonisins and Fusarin C
T-2 toxin
f .4 Detection and identification of toxigenic Fusarium species ............................. 25
1.4.1 Identification of Fusarium species using morphological parameters..... 25
1.4.2 Identification of Fusarium species using selective media ................ 26
1.4.3 Identification of Fusarium species using secondary metabolites .......... 26
1.4.4 Identification of Fusarium species using ELISA .................................. 26
1.4.5 Identification of Fusarium species using PCR ...................................... 27
1.5 General methods for the DNA extraction for PCR identification ..................... 29
1.5.1 Quantitative identification using PCR .................................................. 31
1.6 Fusarium on malt ............................................................................................... 31
1.6.1 Introduction ............................................................................................ 31
1.6.2 Gushing .................................................................................................. 32
1.6.3 Identification of fungal species on malt................................................. 33
1.7 Other Quality relevant fungi infecting cereals and malts ............................ 34
1.7.1 Aflatoxins.............................................................................................. 34
1.7.2 Ochratoxin A ........................................................................................ 34
1.7.3 Identification and detection of aflatoxigenic and OTA producing
fungi .......................................................................................... 35
1.7.4 Mycotoxins as agent for biological weapons ........................................ 36
1.8 References ........................................................................................................ 37
2. Objectives of the studies .......................................................................................... 59
3. Use of Ultrasonication for the rapid preparation of Fusarium DNA from Cereals and its
detection by PCR. Mulfinger S., Niessen L., Vogel R.F., (1999) ......................... 62
3.1 Introduction ................................................................................................ 64
3.2 Materials and Methods ............................................................................... 65
3.3 Results ........................................................................................................ 68
5
3.4 Discussion .................................................................................................. 71
3.5 References ................................................................................................... 75
Figures ............................................................................................................... 78
4. Detection and identification of toxigenic fungal species on malt - a new method for quality
control in the brewery. Mulfinger S., Niessen L., Vogel R., (2000) Advances in Food
Science 22 (1/2): 38 - 46. 78 ..................................................................................... 84
4.1 Introduction ................................................................................................. 86
4.2 Materials and Methods ................................................................................ 87
4.3 Results ......................................................................................................... 89
4.4 Discussion ................................................................................................... 92
4.5 References ................................................................................................... 95
Figures ............................................................................................................... 98
5. Detection of aflatoxigenic fungi in wheat by PCR Geisen R., Mulfinger S., Niessen L.,
(1999) Journal of Food Mycology 1 (4): 211-218 ............................................ 105
5.1 Introduction ................................................................................................. 107
5.2 Materials and Methods ................................................................................ 107
5.3 Results ......................................................................................................... 109
5.4Discussion ................................................................................................... Ill
5.5 References ................................................................................................... 112
Figures ............................................................................................................... 113
6. Conclusions ............................................................................................................... 116
6.1 Introduction ................................................................................................. 117
6.2 Molecular detection and identification of toxigenic fungi using PCR ....... 117
6.3 Identification of Fusarium spp. on cereals ................................................. 118
6.4 Identification of Fusarium spp. on malt ..................................................... 118
6.4 Identification of aflatoxigenic fungi on cereals .......................................... 119
7. Deutsche Zusammenfassung .................................................................................... 120
7.1 Einleitung .................................................................................................... 121
7.2 Detektion und Identifizierung von toxigenen Fusarien mit Hilfe
derPCR ................................................................................................. 122
7.3 Identifizierung von Fusarien aufGetreide .................................................. 122
7.4 Identifizierung von Fusarien auf Malz ....................................................... 123
7.5 Identifizierung von aflatoxigenen Pilzen aufGetreide ............................... 124
7.6 Schlufifolgerung ......................................................................................... 124
Acknowledgements ...................................................................................................... 125
|
| any_adam_object | 1 |
| author | Mulfinger, Sven |
| author_facet | Mulfinger, Sven |
| author_role | aut |
| author_sort | Mulfinger, Sven |
| author_variant | s m sm |
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| classification_tum | LEB 310d BRA 430d LEB 022d BRA 290d |
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| discipline | Brauwesen Lebensmitteltechnologie |
| format | Book |
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| spelling | Mulfinger, Sven Verfasser aut Development and application of PCR-based diagnostic assays for toxigenic fungi in cereals and malts Sven Mulfinger Stuttgart Grauer 2000 126 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zugl.: München, Techn. Univ., Diss., 2000 Malz (DE-588)4168742-5 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf Getreide (DE-588)4121356-7 gnd rswk-swf Fusarium (DE-588)4155723-2 gnd rswk-swf Nachweis (DE-588)4115334-0 gnd rswk-swf Mykotoxin (DE-588)4040984-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Getreide (DE-588)4121356-7 s Mykotoxin (DE-588)4040984-3 s Nachweis (DE-588)4115334-0 s Polymerase-Kettenreaktion (DE-588)4256726-9 s DE-604 Malz (DE-588)4168742-5 s Fusarium (DE-588)4155723-2 s HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009121656&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mulfinger, Sven Development and application of PCR-based diagnostic assays for toxigenic fungi in cereals and malts Malz (DE-588)4168742-5 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Getreide (DE-588)4121356-7 gnd Fusarium (DE-588)4155723-2 gnd Nachweis (DE-588)4115334-0 gnd Mykotoxin (DE-588)4040984-3 gnd |
| subject_GND | (DE-588)4168742-5 (DE-588)4256726-9 (DE-588)4121356-7 (DE-588)4155723-2 (DE-588)4115334-0 (DE-588)4040984-3 (DE-588)4113937-9 |
| title | Development and application of PCR-based diagnostic assays for toxigenic fungi in cereals and malts |
| title_auth | Development and application of PCR-based diagnostic assays for toxigenic fungi in cereals and malts |
| title_exact_search | Development and application of PCR-based diagnostic assays for toxigenic fungi in cereals and malts |
| title_full | Development and application of PCR-based diagnostic assays for toxigenic fungi in cereals and malts Sven Mulfinger |
| title_fullStr | Development and application of PCR-based diagnostic assays for toxigenic fungi in cereals and malts Sven Mulfinger |
| title_full_unstemmed | Development and application of PCR-based diagnostic assays for toxigenic fungi in cereals and malts Sven Mulfinger |
| title_short | Development and application of PCR-based diagnostic assays for toxigenic fungi in cereals and malts |
| title_sort | development and application of pcr based diagnostic assays for toxigenic fungi in cereals and malts |
| topic | Malz (DE-588)4168742-5 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Getreide (DE-588)4121356-7 gnd Fusarium (DE-588)4155723-2 gnd Nachweis (DE-588)4115334-0 gnd Mykotoxin (DE-588)4040984-3 gnd |
| topic_facet | Malz Polymerase-Kettenreaktion Getreide Fusarium Nachweis Mykotoxin Hochschulschrift |
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