Assessment of tropical plants for estrogenic activity and principles:
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| Main Author: | |
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2000
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 177 Bl. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a Assessment of tropical plants for estrogenic activity and principles |c vorgelegt von Yusuf W. Akanni |
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| 502 | |a Kaiserslautern, Univ., Diss., 2000 | ||
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| _version_ | 1804128104086503424 |
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| adam_text | ASSESSMENT OF TROPICAL PLANTS FOR ESTROGENIC ACTIVITY AND PRINCIPLES VOM
FACHBEREICH CHEMIE DER UNIVERSITAT KAISERSLAUTERN ZUR VERLEIHUNG DES
AKADEMISCHEN GRADES DOKTOR DER NATURWISSENSCHAFTEN GENEHMIGTE
DISSERTATION (D386) VORGELEGT VON BIOCHEMIKER YUSUF W. AKANNI BETREUER:
PROF. DR. G. EISENBRAND KAISERSLAUTERN, 2000 CONTENTS INTRODUCTION 1 1.1
ENDOCRINE DISRUPTORS 1 1.1.1 ENDOCRINE DISRUPTORS AND WILDLIFE: FIELD /
ENVIRONMENTAL OBSERVATIONS 1 1.1.1.2 ENDOCRINE DISRUPTORS: CORRELATION
BETWEEN FIELD AND LABORATORY OBSERVATIONS 3 1.1.2 ENDOCRINE DISRUPTORS
AND HUMAN HEALTH: EPIDEMIOLOGICAL REPORTS 3 -- 1.1.2.1 SPERM QUALITY AND
QUANTITY: INFLUENCE OF ANTHROPOGENIC AND NATURAL AGENTS 4 1.1.2.2 OTHER
REPRODUCTIVE HEALTH PROBLEMS FOR WHICH XENOESTROGENS HAVE BEEN
IMPLICATED 6 1.1.2.3 BREAST CANCER INCIDENCE: EVIDENCE OF UPWARD TREND
AND XENOESTROGEN CONNECTION 7 THEORETICAL PART 10 2.1 HORMONES 10 2.1.1
CLASSIFICATION OF HORMONES 10 2.1.2 CORTICOSTEROIDS 11 2.1.3 SEX
HORMONES: ANDROGENS AND ESTROGENS 12 2.1.3.1 ANDROGENS 12 2.1.3.2
ESTROGENS 14 2.1.4 SOURCES OF CHOLESTEROL FOR STEROID HORMONE
BIOSYNTHESIS 15 2.1.5 BIOSYNTHESIS OF STEROID HORMONES 16 2.1.6
REGULATION OF HORMONAL SECRETION 17 2.1.7 FATE OF STEROID HORMONES 19
2.1.7.1 CORTICOSTEROID BINDING GLOBULIN (CBG) 20 2.1.7.2 ALBUMIN 20
2.1.7.3 SEX HORMONE BINDING GLOBULIN 21 2.1.7.4 CATABOLISM AND EXCRETION
OF ESTROGENS 22 2.2 ENVIRONMENTAL ESTROGENS 24 2.2.1 SYNTHETIC ESTROGENS
AND HEALTH IMPLICATIONS 24 2.2.1.1 POIYCHLORINATED BIPHENYLS (PCBS) 24
2.2.1.2 DICHLORODIPHENYLTRICHLOROETHANE (DDT) 25 2.2.1.3
ALKYLPHENOL(POLY)ETHOXYLATES 26 2.2.1.4 POLYCARBONATES 27 2.2.1.5
PHTHALATES 27 2.2.1.6 KEPONE 28 2.2.1.7 ORGANIC TIN COMPOUNDS 29 2.3
PHYTOESTROGENS 30 2.3.1 ISOFLAVONES 31 2.3.2 LIGNANS 32 2.3.3
PHYSIOLOGIC EFFECTS OF PHYTOESTROGENS AND IMPLICATIONS FOR HEALTH AND
DISEASE 32 2.3.3.1 ANTICARCINOGENIC ACTIVITY OF PHYTOESTROGENS IN
ER-NEGATIVE CELLS 34 2.3.3.2 PHYTOESTROGENS AND CARDIOVASCULAR
DISEASES....34 2.3.3.3 THE OTHER SIDE OF PHYTOESTROGENS: EFFECTS ON
MENSTRUAL CYCLE 36 2.4 ANTIESTROGENS 36 2.5.1 MECHANISM OF ACTION OF
PHYSIOLOGICAL ESTROGENS 38 2.5.2 LIGAND-INDEPENDENT STEROID RECEPTOR
ACTIVATION 41 2.5.3 HEAT SHOCK PROTEIN MR 90,000 (HSP 90) AND STEROID
HORMONE RECEPTORS 42 2.6 ASSESSMENT OF ESTROGENIC ACTIVITY OF CHEMICAL
COMPOUNDS AND NATURAL PRODUCTS : 43 2.6.1 IN VIVO METHODS 44 2.6.1.1
UTEROTROPHIC ASSAY 44 2.6.1.2 OTHER IN VIVO ASSAY PROCEDURES 44 2.6.2 IN
VITRO ASSAYS 45 2.6.2.1 THE E-SCREEN ASSAY 46 2.6.2.2 REPORTER GENE
ASSAY 47 2.6.2.3 RECEPTOR BINDING ASSAY (RBA) 51 2.6.2.4 INDUCTION OF
PROGESTERONE RECEPTOR AND PS2 ....51 3 OBJECTIVE OF THE PRESENT WORK 52
4 RESULTS AND DISCUSSION 54 4.1 VERIFICATION OF THREE IN VITRO METHODS
OF ESTROGENICITY MEASUREMENT 54 4.1.1 THE E-SCREEN ASSAY 54 4.1.1.1
E-SCREEN ASSAY OF B-ESTRADIOL AND DIETHYLSTILBESTROL 55 4.1.1.2
VALIDATION OF ESTROGENIC ACTIVITY OF SOME STILBENES USING THE E-SCREEN
ASSAY 57 4.1.2 TEST FOR SUITABILITY OF BAND-SHIFT (GEL MOBILITY SHIFT)
METHOD AS AN ESTROGENICITY ASSAY 62 4.1.2.1 BAND-SHIFT ASSAY OF
B-ESTRADIOL AND DIETHYLSTILBESTROL (IN VIVO AND IN VITRO) 64 4.1.2.2
BAND-SHIFT ASSAY WITH ANTIESTROGEN ICI 182780 67 4.1.3 VALIDATION OF
LUCIFERASE REPORTER GENE ASSAY 68 4.1.3.1 PRINCIPLE OF LUCIFERASE
REPORTER GENE ASSAY 68 4.1.3.2 ESTROGENIC ACTIVITY OF B-ESTRADIOL AND
DIETHYLSTILBESTROL IN REPORTER GENE ASSAY 72 4.1.3.3 REPORTER GENE ASSAY
OF STILBENES 73 4.2 INVESTIGATING EXTRACTS OF MEDICINAL PLANTS FOR
ESTROGENIC ACTIVITY IN VITRO 76 4.2.1 EXTRACTION OF PLANT SAMPLES 78
4.2.2 REPORTER GENE ASSAY OF PLANT EXTRACTS AND THEIR CHROMATOGRAPHIC
FRACTIONS 79 4.2.3 E-SCREEN ASSAY OF PLANT EXTRACTS AND THEIR
CHROMATOGRAPHIC FRACTIONS 82 4.2.4 EXTRACTION OF PHYTOESTROGENS (WANG ET
AI, 1994)-MODIFIED. .85 4.2.4.1 DETECTION OF ESTROGENICITY IN AN EXTRACT
OF GARCINIA KOLA AND ITS ENHANCEMENT OF ESTROGENIC ACTIVITY OF
B-ESTRADIOL 87 4.2.4.2 MODIFYING EFFECT OF EXTRACT OF G. KOLA ON
ESTROGENIC ACTIVITY OF XENOESTROGENS 93 4.2.4.3 REPORTER GENE ASSAY OF
BINARY MIXTURES OF XENOESTROGENS 95 4.2.4.4 ATTEMPTS AT CHARACTERIZING
ESTROGENIC CONSTITUENTS OF G. KOLA 98 4.2.4.5 INFRA RED SPECTROSCOPY OF
ESTROGENIC TIC FRACTION 3B OF G. KOLA AND DAIDZEIN 104 4.2.4.6 HPLC
ANALYSIS OF ESTROGENIC FRACTION 3B OF G. KOLA AND STANDARD
PHYTOESTROGENS 106 4.2.4.7 CONSUMPTION OF SEEDS OF G. KOLA: RISK
ASSESSMENT 110 4.2.5 DEVELOPMENT OF BIO- AND INSTRUMENTAL ANALYTICAL
PROCEDURE FOR DETECTION OF PHYTOESTROGENS 111 4.2.5.1 CHROMATOGRAPHIC
DETECTION OF PHYTOESTROGENS IN TROPICAL GRASSES 119 4.2.5.2 DETECTION OF
UNCONJUGATED PHYTOESTROGENS IN TROPICAL GRASSES 120 4.2.5.3 TOTAL
PHYTOESTROGENS DETECTED IN TROPICAL GRASSES 123 SUMMARY /
ZUSAMMENFASSUNG 126 5.1 SUMMARY 126 5.2 ZUSAMMENFASSUNG 129 MATERIALS
AND METHOD 132 6.1 EQUIPMENT 132 6.2.1 MATERIALS 133 6.2.2 CELL LINES
133 6.2.2.1 MAMMARY CARCINOMA (MCF-7) CELLS 133 6.2.2.2 MCF-7 LUCIFERASE
(MVLN-2B) CELLS 133 6.2.2.3 A 431 CELLS 134 6.2.2.4 CACO-2 CELLS 134
6.2.2.5 COS-1 CELL LINE 134 6.2.3.1 CHEMICALS AND SOLUTION 134 6.2.3.2
GROWTH AND MAINTAINANCE OF CELLS 135 6.2.3.3 CHANGE OF CULTURE MEDIA 136
6.2.3.4 PASSAGING OF CELLS 136 6.2.3.5 FREEZING OF CELLS 136 6.2.3.6
DEFREEZING OF CELLS 136 6.2.3.7 COUNTING OF CELLS 137 6.2.4 DETECTION
AND ELIMINATION OF MYCOPLASMA 137 6.2.4.1 MATERIALS FOR DETECTION OF
MYCOPLASMA 137 6.2.4.2 ELIMINATION OF MYCOPLASMA 138 6.3 ESTROGEN
SCREENING (E-SCREEN) ASSAY 138 6.3.1 MATERIALS AND EQUIPMENT 138 6.3.2
METHOD 138 6.4 LUCIFERASE REPORTER GENE ASSAY 140 6.4.1 REAGENTS 140
6.4.1.1 PROMEGA-SUPPLIED REAGENTS 140 6.4.1.2 BERTHOLD-SUPPLIED REAGENTS
140 6.4.2 MATERIALS/EQUIPMENT 140 6.4.3 METHOD 141 6.4.3.1 INCUBATION OF
MCF-7LUC (MVLN-2B) CELLS WITH TEST SUBSTANCES 141 6.4.3.2 LYSIS OF
MCF-7LUC CELLS 141 6.4.3.3 LUMINISCENCE MEASUREMENT OF CELL LYSATE 142
6.5 BAND-SHIFT (GEL MOBILITY SHIFT) ASSAY 143 6.5.1 INCUBATION OF CELLS
AND ISOLATION OF NUCLEAR EXTRACTS 143 6.5.1.1 MATERIALS 143 6.5.1.2
METHOD 143 6.5.2 ELECTROPHORESIS WITH NATIVE POLYACRYLAMIDE GEL 144
6.5.2.1 MATERIALS/APPARATUS 144 6.5.2.2 METHOD 145 6.5.3 BLOTTING AND
DETECTION 146 6.5.3.1 MATERIALS 146 6.5.3.2 METHOD 147 6.5.4
END-LABELLING OF OLIGONUCLEOTIDE WITH DIG-11-DDUTP 148 6.5.4.1
MATERIALS...!.. 148 6.5.4.2 METHOD 149 6.5.5 PROTEIN DETERMINATION
(BCA-PROTEIN ASSAY) 149 6.5.5.1 MATERIALS 149 6.5.5.2 METHOD 150 6.6
EXTRACTION OF PLANT SAMPLES 150 6.6.1 MATERIALS 150 6.6.2 GENERAL SCHEME
OF EXTRACTION OF PLANT SAMPLES 150 6.6.2.1 EXTRACTION OF PHYTOESTROGENS
(WANG ET AL, 1994)-MODIFIED 150 6.6.2.2 EXTRACTION OF PHYTOESTROGENS
(SETCHELL ET AL, 1987)-MODIFIED 151 6.6.3 PURIFICATION OF PLANT EXTRACTS
151 6.6.3.1 CHROMATOGRAPHY ON POLYACRYLAMIDE COLUMN....151 6.6.3.2
CHROMATOGRAPHY ON SILICA GEL 60 COLUMN 152 6.6.3.3 THIN LAYER
CHROMATOGRAPHY (TLC) (PREPARATIVE) 152 6.6.3.4 SOLID PHASE EXTRACTION
(SPE) 153 6.6.4 HPLC OF PLANT EXTRACTS/FRACTIONS 153 6.6.5 PREPARATION
OF SAMPLES FOR FTIR SPECTROSCOPY 154 REFERENCES 155
|
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| indexdate | 2024-07-09T18:43:56Z |
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| physical | 177 Bl. Ill., graph. Darst. |
| publishDate | 2000 |
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| spelling | Akanni, Yusuf W. 1967- Verfasser (DE-588)122359003 aut Assessment of tropical plants for estrogenic activity and principles vorgelegt von Yusuf W. Akanni 2000 177 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Kaiserslautern, Univ., Diss., 2000 Östrogenstoffwechsel (DE-588)4172468-9 gnd rswk-swf Tropische Pflanzen (DE-588)4332622-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Tropische Pflanzen (DE-588)4332622-5 s Östrogenstoffwechsel (DE-588)4172468-9 s DE-604 GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009090463&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Akanni, Yusuf W. 1967- Assessment of tropical plants for estrogenic activity and principles Östrogenstoffwechsel (DE-588)4172468-9 gnd Tropische Pflanzen (DE-588)4332622-5 gnd |
| subject_GND | (DE-588)4172468-9 (DE-588)4332622-5 (DE-588)4113937-9 |
| title | Assessment of tropical plants for estrogenic activity and principles |
| title_auth | Assessment of tropical plants for estrogenic activity and principles |
| title_exact_search | Assessment of tropical plants for estrogenic activity and principles |
| title_full | Assessment of tropical plants for estrogenic activity and principles vorgelegt von Yusuf W. Akanni |
| title_fullStr | Assessment of tropical plants for estrogenic activity and principles vorgelegt von Yusuf W. Akanni |
| title_full_unstemmed | Assessment of tropical plants for estrogenic activity and principles vorgelegt von Yusuf W. Akanni |
| title_short | Assessment of tropical plants for estrogenic activity and principles |
| title_sort | assessment of tropical plants for estrogenic activity and principles |
| topic | Östrogenstoffwechsel (DE-588)4172468-9 gnd Tropische Pflanzen (DE-588)4332622-5 gnd |
| topic_facet | Östrogenstoffwechsel Tropische Pflanzen Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009090463&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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