On the activation and regulation of the silent Escherichia coli bgl promoter:
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| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
2000
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Köln, Univ., Diss., 1999 |
| Physical Description: | 77 S. Ill., graph. Darst. : 21 cm |
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MARC
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| 100 | 1 | |a Caramel, Angela |d 1967- |e Verfasser |0 (DE-588)121993728 |4 aut | |
| 245 | 1 | 0 | |a On the activation and regulation of the silent Escherichia coli bgl promoter |c vorgelegt von Angela Caramel |
| 264 | 1 | |c 2000 | |
| 300 | |a 77 S. |b Ill., graph. Darst. : 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Köln, Univ., Diss., 1999 | ||
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009018205 | |
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| _version_ | 1807593638062981120 |
|---|---|
| adam_text |
CONTENTS
I
ABBREVIATIONS
IT*
ZUSAMMENFASSUNG
I.
SUMMARY
1
II.
INTRODUCTION
3
1.
THE
CRYPTIC
BGL
OPERON
OF
ESCHERICHIA
COLI,
A
COMPLEX
SYSTEM
3
2.
STRUCTURE
AND
FI-GLUCOSIDE-SPECIFIC
REGULATION
OF
THE
BGL
OPERON
3
3.
ACTIVATION
OF
THE
CRYPTIC
E.
COLI
BGL
OPERON
BY
SPONTANEOUS
MUTATIONS
6
4.
SILENCING
OF
THE
BGL
PROMOTER
BY
FLANKING
SEQUENCE
ELEMENTS
7
5.
H-NS,
A
HISTONE-LIKE
PROTEIN,
INVOLVED
IN
SILENCING
OF
THE
BGL
PROMOTER
8
6.
SILENCING
OF
THE
BGL
PROMOTER
BY
A
PUTATIVE
NUCLEOPROTEIN
COMPLEX
9
7.
HISTONE-LIKE
PROTEIN
FIS
9
8.
OTHER
SYSTEMS
INVOLVED
IN
^-GLUCOSIDE
UTILIZATION
IN
E.
COLI
10
9.
THESIS
GOAL
12
III.
RESULTS
14
1.
STUDIES
ON
THE
MECHANISM
OF
BGL
PROMOTER
ACTIVATION.
EFFECTS
OF
14
MODIFICATION
OF
THE
LOCAL
CHROMATIN
STRUCTURE
BY
PROVIDING
SPECIFIC
BINDING
OF
LAC
AND
1
CIGS?
REPRESSORS
WITHIN
THE
UPSTREAM
SILENCER
SEQUENCE.
1.1.
BINDING
OF
LAC
REPRESSOR
WITHIN
THE
BGL
UPSTREAM
SILENCER
REGION
LEADS
16
TO
ACTIVATION
OF
THE
BGL
PROMOTER
1.2.
ACTIVATION
OF
THE
BGL
PROMOTER
BY
LAC
REPRESSOR
IS
PHASE-INDEPENDENT
19
1.3.
BINDING
OF
X
CLGG?
REPRESSOR
TO
O
R
I
O
R
2
WITHIN
THE
BGL
UPSTREAM
20
SILENCER
REGION
LEADS
TO
ACTIVATION
OF
THE
BGL
PROMOTER
2.
STUDIES
ON
THE
ROLE
OF
HISTONE-LIKE
PROTEIN
FIS
IN
BGL
PROMOTER
REGULATION
22
2.1.
FIS
BINDS
SPECIFICALLY
TO
THE
BGL
PROMOTER
REGION
IN
VITRO
22
2.2
FIS
AND
CAP
COMPETE
FOR
BINDING
TO
OVERLAPPING
BINDING
SITES
AT
THE
BGL
26
PROMOTER
2.3
FIS-MEDIATED
REPRESSION
OF
THE
BGL
PROMOTER
IS
ANTAGONIZED
BY
CAP
28
2.4
FIS
AND
RNA
POLYMERASE
BIND
SIMULTANEOUSLY
TO
THE
BGL
PROMOTER
REGION
30
2.5
FIS
INHIBITS
OPEN
COMPLEX
FORMATION
BY
RNA
POLYMERASE
32
IV.
DISCUSSION
35
1.
ACTIVATION
OF
THE
BGL
PROMOTER
BY
SPECIFIC
BINDING
OF
LAC
AND
1
CIGS?
35
REPRESSORS
WITHIN
THE
UPSTREAM
SILENCER
REGION
IS
LIKELY
TO
BE
MEDIATED
VIA
THE
ALTERATION
OF
A
REPRESSING
NUCLEOPROTEIN
COMPLEX
2.
INVOLVEMENT
OF
HISTONE-LIKE
PROTEIN
FIS
IN
THE
REGULATION
OF
THE
BGL
37
PROMOTER
3.
HOW
IS
THE
BGL
PROMOTER
KEPT
SILENT?
42
4.
OUTLOOK
45
V.
MATERIALS
47
1.
CHEMICALS,
ENZYMES
AND
OTHER
MATERIALS
47
2.
MEDIA
AND
AGAR
PLATES
47
3.
SYNTHETIC
OLIGONUCLEOTIDES
48
4.
BACTERIAL
STRAINS
50
5.
PLASMIDS
50
6.
PROTEINS
55
VI.
METHODS
56
1.
RECOMBINANT
DNA
TECHNIQUES
56
1.1.1.
ENZYME
CATALYZED
REACTIONS
56
1.1.2.
PCR
56
1.3.
ISOLATION
OF
PLASMID
DNA
FROM
E.
COLL
56
1.3.1.
LARGE
SCALE
PREPARATIONS
OF
PLASMID
DNA
56
1.3.2.
SMALL
SCALE
PREPARATIONS
OF
PLASMID
DNA
56
1.3.3.
ELUTION
OF
DNA
FRAGMENTS
FROM
AGAROSE
GELS
57
1.4.
PLASMIDS
AND
DNA
FRAGMENTS
57
1.4.1
CONSTRUCTION
OF
PLASMIDS
57
1.4.2.
PCR
FRAGMENTS
USED
IN
THE
IN
VITRO
TRANSCRIPTION
ASSAYS
58
1.4.3
PCR
FRAGMENTS
USED
IN
DNASE
I
FOOTPRINTING
AND
GEL
SHIFT
ASSAYS
58
2.
TRANSFORMATION
OF
E.
COLI
58
2.1.
PREPARATION
OF
COMPETENT
CELLS
59
2.2.
TRANSFORMATION
OF
COMPETENT
CELLS
59
3.
CONSTRUCTION
OF
STRAINS
59
3.1.
CHROMOSOMAL
DELETION
OF
THE
BGL
OPERON
59
3.2.
INTEGRATION
OF
DNA
INTO
THE
E.
COLI
CHROMOSOME
60
4.
DETERMINATION
OF
^-GALACTOSIDASE
ENZYME
ACTIVITY
61
5.
DNA
SEQUENCING
61
6.
RADIOACTIVE
LABELLING
OF
DNA
FRAGMENTS
62
7.
DNASE
I
FOOTPRINTING
62
8.
IN
VITRO
TRANSCRIPTION
ASSAY
63
9.
GEL
SHIFT
ASSAY
63
VII.
REFERENCE
LIST
65
VIII.
TABLES
AND
FIGURES
75
IX.
APPENDIX
76 |
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| author_facet | Caramel, Angela 1967- |
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| indexdate | 2024-08-17T00:47:05Z |
| institution | BVB |
| language | English |
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| oclc_num | 49877059 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 77 S. Ill., graph. Darst. : 21 cm |
| publishDate | 2000 |
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| spelling | Caramel, Angela 1967- Verfasser (DE-588)121993728 aut On the activation and regulation of the silent Escherichia coli bgl promoter vorgelegt von Angela Caramel 2000 77 S. Ill., graph. Darst. : 21 cm txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 1999 Promotor Genetik (DE-588)4263977-3 gnd rswk-swf Escherichia coli (DE-588)4070959-0 gnd rswk-swf Aktivierung Chemie (DE-588)4141761-6 gnd rswk-swf Glucoside (DE-588)4392927-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Escherichia coli (DE-588)4070959-0 s Glucoside (DE-588)4392927-8 s Promotor Genetik (DE-588)4263977-3 s Aktivierung Chemie (DE-588)4141761-6 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009018205&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Caramel, Angela 1967- On the activation and regulation of the silent Escherichia coli bgl promoter Promotor Genetik (DE-588)4263977-3 gnd Escherichia coli (DE-588)4070959-0 gnd Aktivierung Chemie (DE-588)4141761-6 gnd Glucoside (DE-588)4392927-8 gnd |
| subject_GND | (DE-588)4263977-3 (DE-588)4070959-0 (DE-588)4141761-6 (DE-588)4392927-8 (DE-588)4113937-9 |
| title | On the activation and regulation of the silent Escherichia coli bgl promoter |
| title_auth | On the activation and regulation of the silent Escherichia coli bgl promoter |
| title_exact_search | On the activation and regulation of the silent Escherichia coli bgl promoter |
| title_full | On the activation and regulation of the silent Escherichia coli bgl promoter vorgelegt von Angela Caramel |
| title_fullStr | On the activation and regulation of the silent Escherichia coli bgl promoter vorgelegt von Angela Caramel |
| title_full_unstemmed | On the activation and regulation of the silent Escherichia coli bgl promoter vorgelegt von Angela Caramel |
| title_short | On the activation and regulation of the silent Escherichia coli bgl promoter |
| title_sort | on the activation and regulation of the silent escherichia coli bgl promoter |
| topic | Promotor Genetik (DE-588)4263977-3 gnd Escherichia coli (DE-588)4070959-0 gnd Aktivierung Chemie (DE-588)4141761-6 gnd Glucoside (DE-588)4392927-8 gnd |
| topic_facet | Promotor Genetik Escherichia coli Aktivierung Chemie Glucoside Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009018205&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT caramelangela ontheactivationandregulationofthesilentescherichiacolibglpromoter |