Molecular studies in transcriptional and translational regulation of potato leafroll luteovirus (PLRV) gene expression via subgenomic RNA synthesis and non-canonical translation initiation:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
1999
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | IV, 67 S. graph. Darst. : 21 cm |
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MARC
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| 100 | 1 | |a Ashoub, Ahmed |d 1967- |e Verfasser |0 (DE-588)121849570 |4 aut | |
| 245 | 1 | 0 | |a Molecular studies in transcriptional and translational regulation of potato leafroll luteovirus (PLRV) gene expression via subgenomic RNA synthesis and non-canonical translation initiation |c vorgelegt von Ahmed Ashoub |
| 264 | 1 | |c 1999 | |
| 300 | |a IV, 67 S. |b graph. Darst. : 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Köln, Univ., Diss., 1999 | ||
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009018060 | |
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| _version_ | 1807319935180865536 |
|---|---|
| adam_text |
INDEX
I
CONTENTS
1.
INTRODUCTION
1
1.1
STRATEGIES
USED
FOR
PLANT
VIRUS
GENE
EXPRESSION
1
1.2
LUTEOVIRIDAE
2
1.2.1
VARIATIONS
BETWEEN
LUTEOVIRUSES
SUBGROUP
1
AND
2
4
1.2.2
STRUCTURE
OF
PLRV
GENOMIC
RNA
4
1.2.3
STUDIES
ON
THE
PROTEINS
CODED
BY
LUTEOVIRUSES
SUBGROUP
2
5
1.2.3.1
STUDIES
ON
ORFO
5
1.2.3.2
STUDIES
ON
ORF1
6
1.2.3.3
STUDIES
ON
ORF2
6
1.2.3.4
STUDIES
ON
ORF3
7
1.2.3.5
STUDIES
ON
ORF4
7
1.2.3.6
STUDIES
ON
ORF5
7
1.4
THE
AIM
OF
THIS
THESIS
8
2.
MATERIALS
AND
METHODS
9
2.1
MATERIALS
9
2.1.1
PLANT
MATERIAL
9
2.1.2
BACTERIAL
STRAINS
9
2.1.2.1
ESCHERICHIA
COLI
9
2.1.2.2
AGROBACTERIUM
TUMEFACIENS
9
2.1.2.3
BACTERIOPHAGE
9
2.1.3
VECTORS
9
2.1.4
CHEMICALS
10
2.1.5
RADIOACTIVITY
10
2.1.6
ENZYMES
10
2.1.7
FILMS
10
2.1.8
KITS
10
2.1.9
FILTERS
10
TOTE
_
2
2.1.10
OLIGONUCLEOTIDES
10
2.1.11
ANTIBODIES
11
2.2
METHODS
11
2.2
CONSTRUCTION
OF
PLASMIDS
11
2.2.1
PRODUCTION
OF
P35SSGRNA2-FL
CONSTRUCT
11
2.2.2
PRODUCTION
OF
CHIMERIC
ORF6-GUS
CONSTRUCT
11
2.2.3
PRODUCTION
OF
CHIMERIC
ORF7-GUS
CONSTRUCTS
12
2.2.4
CONSTRUCTION
OF
PLASMIDS
FOR
PROTEIN
EXPRESSION
12
2.2.5
CONSTRUCTION
OF
P65SGRNA2-FL
'
12
2.2.6
CONSTRUCTION
OF
P65O6
13
2.2.7
CONSTRUCTION
OF
P65O7
13
2.2.8
CONSTRUCTION
OF
PLASMIDS
FOR
IN
VIRRO-MUTAGENSIS
13
2.2.9
CONSTRUCTION
OF
PLASMIDS
FOR
PLRV
SGRNA2
PLANT
TRANSFORMATION
13
2.2.10
CONSTRUCTION
OF
PLASMIDS
FOR
PLRV
ORF6
PLANT
TRANSFORMATION
14
2.3
RNA
DETECTION
14
2.3.1
POLYSOMAL
RNA
EXTRACTION
14
2.3.2
AFFINITY
PURIFICATION
OF
PLRV
SGRNA2
15
2.3.3
NORTHERN
BLOT
ANALYSIS
16
2.3.4
PREPARATION
AND
LABELLING
OF
DNA
PROBES
17
2.3.5
PRIMER
EXTENSION
EXPERIMENT
17
2.4
TRANSIENT
EXPRESSION
19
2.4.1
PREPARATION
OF
POTATO
PROTOPLASTS
19
2.4.2
PROTOPLASTS
TRANSFECTION
20
2.4.3
GUS
ASSAY
20
2.5
WESTERN
BLOT
ANALYSIS
21
2.6
PROTEIN
OVER-EXPRESSION
AND
PURIFICATION
23
2.7
NUCLEIC
ACID-BINDING
ASSAY
22
2.8
IN
VITRO-TRANSCRIPTION
23
2.9
IN
VIFRO-TRANSLATION
24
2.10
IMMUNOPRECIPITATION
OF
ORF6
AND
ORF7
24
2.11
PLANT
TRANSFORMATION
AND
ANALYSIS
OF
TRANSGENIC
POTATO
PLANTS
25
LADES
_
S
2.12
ADDITIONAL
METHODS
25
3.
RESULTS
26
3.1
CHARACTERISATION
OF
PLRV
SUBGENOMIC
RNA2
(SGRNA2)
BY
NORTHERN
BLOT
ANALYSIS
26
3.2
IDENTIFICATION
OF
PLRV
SGRNA2
3
'
END
BY
AFFINITY
PURIFICATION
28
3.3
MAPPING
THE
PLRV
SGRNA2
TRANSCRIPTIONAL
START
POINT
28
3.4
MAPPING
THE
CABYV
SGRNA2
TRANSCRIPTIONAL
START
POINT
30
3.5
CHARACTERISATION
OF
PLRV
ORF6
AND
ORF7
BY
BACTERIAL
EXPRESSION
30
3.6
IN
VITRO-TRANSLATION
OF
PLRV
SGRNA2
31
3.7
MUTATIONAL
ANALYSIS
OF
ORF6
33
3.8
MUTATIONAL
ANALYSIS
OF
ORF7
35
3.9
TIME
COURSE
OF
ORF6
AND
NON-AUG
ORF7
EXPRESSION
36
3.10
CHARACTERISATION
OF
PLRV
ORF6
AND
ORF7
AS
AUG
AND
NON-AUG
PRODUCTS
BY
TRANSIENT
EXPRESSION
OF
CHIMERIC
PLRV
ORF/GUS
CONSTRUCTS
37
3.11
WESTERN
BLOT
ANALYSIS
OF
PLRV
INFECTED
PLANTS
AND
PLRV
SGRNA2
TRANSGENIC
PLANTS
40
4.
DISCUSSION
42
4.1
IDENTIFICATION
OF
PLRV
SGRNA2
42
4.1.1
IDENTIFICATION
OF
PLRV
AND
CABYV
SGRNA2
BY
NORTHERN
BLOTTING,
PRIMER
EXTENSION
AND
AFFINITY
PURIFICATION
OF
PLRV
SGRNA2
42
4.2
CHARACTERISATION
OF
ORF6
AND
ORF7
BY
TRANSIENT
EXPRESSION
IN
POTATO
PROTOPLASTS
AND
NUCLEIC
ACID-BINDING
ASSAY
45
4.3
MUTATIONAL
ANALYSIS
OF
ORF6
46
4.4
IDENTIFICATION
OF
ORF7
INITIATION
CODON
47
4.5
IN
VIVO-DETECTION
OF
ORF7UUO
49
4.6
SPECULATIONS
ABOUT
THE
FUNCTION
OF
ORF7
AUO
51
INDEX
IV
5.
SUMMARY
53
6.
REFERENCES
57 |
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| indexdate | 2024-08-14T00:16:42Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009018060 |
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| physical | IV, 67 S. graph. Darst. : 21 cm |
| publishDate | 1999 |
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| spelling | Ashoub, Ahmed 1967- Verfasser (DE-588)121849570 aut Molecular studies in transcriptional and translational regulation of potato leafroll luteovirus (PLRV) gene expression via subgenomic RNA synthesis and non-canonical translation initiation vorgelegt von Ahmed Ashoub 1999 IV, 67 S. graph. Darst. : 21 cm txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 1999 Kartoffel (DE-588)4029804-8 gnd rswk-swf RNS-Synthese (DE-588)4390988-7 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf Kartoffelblattrollvirus (DE-588)4303282-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Kartoffel (DE-588)4029804-8 s Kartoffelblattrollvirus (DE-588)4303282-5 s Genexpression (DE-588)4020136-3 s RNS-Synthese (DE-588)4390988-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009018060&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ashoub, Ahmed 1967- Molecular studies in transcriptional and translational regulation of potato leafroll luteovirus (PLRV) gene expression via subgenomic RNA synthesis and non-canonical translation initiation Kartoffel (DE-588)4029804-8 gnd RNS-Synthese (DE-588)4390988-7 gnd Genexpression (DE-588)4020136-3 gnd Kartoffelblattrollvirus (DE-588)4303282-5 gnd |
| subject_GND | (DE-588)4029804-8 (DE-588)4390988-7 (DE-588)4020136-3 (DE-588)4303282-5 (DE-588)4113937-9 |
| title | Molecular studies in transcriptional and translational regulation of potato leafroll luteovirus (PLRV) gene expression via subgenomic RNA synthesis and non-canonical translation initiation |
| title_auth | Molecular studies in transcriptional and translational regulation of potato leafroll luteovirus (PLRV) gene expression via subgenomic RNA synthesis and non-canonical translation initiation |
| title_exact_search | Molecular studies in transcriptional and translational regulation of potato leafroll luteovirus (PLRV) gene expression via subgenomic RNA synthesis and non-canonical translation initiation |
| title_full | Molecular studies in transcriptional and translational regulation of potato leafroll luteovirus (PLRV) gene expression via subgenomic RNA synthesis and non-canonical translation initiation vorgelegt von Ahmed Ashoub |
| title_fullStr | Molecular studies in transcriptional and translational regulation of potato leafroll luteovirus (PLRV) gene expression via subgenomic RNA synthesis and non-canonical translation initiation vorgelegt von Ahmed Ashoub |
| title_full_unstemmed | Molecular studies in transcriptional and translational regulation of potato leafroll luteovirus (PLRV) gene expression via subgenomic RNA synthesis and non-canonical translation initiation vorgelegt von Ahmed Ashoub |
| title_short | Molecular studies in transcriptional and translational regulation of potato leafroll luteovirus (PLRV) gene expression via subgenomic RNA synthesis and non-canonical translation initiation |
| title_sort | molecular studies in transcriptional and translational regulation of potato leafroll luteovirus plrv gene expression via subgenomic rna synthesis and non canonical translation initiation |
| topic | Kartoffel (DE-588)4029804-8 gnd RNS-Synthese (DE-588)4390988-7 gnd Genexpression (DE-588)4020136-3 gnd Kartoffelblattrollvirus (DE-588)4303282-5 gnd |
| topic_facet | Kartoffel RNS-Synthese Genexpression Kartoffelblattrollvirus Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009018060&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT ashoubahmed molecularstudiesintranscriptionalandtranslationalregulationofpotatoleafrollluteovirusplrvgeneexpressionviasubgenomicrnasynthesisandnoncanonicaltranslationinitiation |