The transcriptional control of virulence gene expression in Helicobacter pylori:
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| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
1999
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Zsfassung in dt. Sprache. - Würzburg, Univ., Diss., 2000 |
| Physical Description: | 106 Bl. Ill., graph. Darst. |
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| 100 | 1 | |a Spohn, Gunther |d 1969- |e Verfasser |0 (DE-588)122383214 |4 aut | |
| 245 | 1 | 0 | |a The transcriptional control of virulence gene expression in Helicobacter pylori |c von Gunther Spohn |
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Record in the Search Index
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|---|---|
| adam_text |
CONTENTS
A. SUMMARY
7
B. INTRODUCTION
II
C. MATERIALS
22
1. BACTERIAL STRAINS.
2. VI CTORS AND PLASMIDS .
3. OLIGONUCLEOTIDES.
4. MEDIA.
5. AGAR PLATES.
6. CHEMICALS AND ENZYMES
7. INSTRUMENTS.
22
22
25
26
27
28
29
D. METHODS.30
1. SMALL AND MIDI SCALE ISOLATION OF PLASMID DNA FROM .
COU.30
2. SMALL AND MIDI SCALE PREPARATION OF CHROMOSOMAL DNA FROM
HELICOBACTER PYLORI.30
3. CLEAVAGE OF DNA WITH RESTRICTION ENDONUCLEASES.31
4. FILLING IN OF DNA ENDS WITH KLENOW ENZYME.32
5. 5'-DEPHOSPHORYLATION OF DNA ENDS WITH ALKALINE PHOSPHATASE.32
6. POLYMERASE CHAIN REACTION.32
7. DEPROTECTION OF OLIGONUCLEOTIDES.33
8. ANALYSIS OF DNA FRAGMENTS HY AGAROSE GEI ELECTROPHORESIS.33
9. SOUTHERN BLOT.34
10. ISOLATION OF DNA FRAGMENTS FROM AGAROSE GELS.34
11. CLONING OF DNA FRAGMENTS. 34
12. PREPARATION OF COMPETENT CELLS OF . COLI STRAINS DH5A AND BL21.35
13. TRANSFORMATION OF
E.
COLI DH5A AND BL21.35
14. TRANSFORMATION OF HELICOBACYER PYLORI STRAINS.35
15. LONG TERM STORAGE OF BACTERIAL STRAINS.36
16. B-GALACTOSIDASE ASSAY.36
17. PURIFICATION OF RECOMBINANT PROTEINS.36
18. PURIFICATION OF THE WILD TYPE AND THE C-TERMINAL TRUNCATED FORM OF
THE: .
COLI RNA POLYMERASE A
SUBUNIT.37
19. ANALYSIS OF PROTEINS BY SDS POLYACRYLAMIDE GEL ELECTROPHORESIS.38
20. COOMASSIE BLUE STAINING OF SDS-POLYACRYLAMIDE GELS.39
21. IMMUNOBLOT (WESTERN BLOT) ANALYSIS.39
22. ANALYSIS OF RADIOACTIVELY LABELED DNA FRAGMENTS BY UREA
POLYACRYLAMIDE GEL ELECTROPHORESIS .40
23. RNA PREPARATION.41
24. 5'-LABELING OF OLIGONUCLEOTIDES.41
25. PRIMER EXTENSION ANALYSIS.41
26. IS VITRO TRANSCRIPTION.42
27. LABELING OF DNA FRAGMENTS AT SPECIFIC RESTRICTION SITES.42
28. SI NUCLEASE MAPPING.43
29. SEQUENCING OF CLONED DNA FRAGMENTS.43
30. END LABELING OF DNA FRAGMENTS WITH KLENOW ENZYME.43
31. ELECTROPHORETIC MOBILITY SHIFT ASSAY.44
32. DNASE I FOOTPRINTING.44
33. G+A SPECIFIC CLEAVAGE OF END LABELED DNA FRAGMENTS.45
5
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/959889337
CONTENTS
E. RESULTS.46
1. TRANSCRIPTIONAL ANALYSIS OF THH DIVERGENT
CAGAB GENES ENCODED BY THE H PYLORI
PATHOGENICITY ISLAND.46
I. I DETERMINATION OF TRANSCRIPTION START SITES
.46
1.2 SPECIFICITY OF PROMOTER RECOGNITION.4 FT
1.3 DELETION ANALYSIS OF THE P1 AND P; PROMOTER REGIONS.4 9
1.4 TRANSCRIPTIONAL ACTIVITY OF THE P? AND P) PROMOTERS IN THE DIFFERENT
MUTANTS.54
1.5 BINDING OF THE A SUBUNIT OF RNA POLYMERASE TO SEQUENCES UPSTREAM OF
THE P, PROMOTER.56
2. TRANSCRIPTIONAL REGULATION OF FLAGELLAR GENES.58
2.1 IDENTIFICATION OF FLAGELLAR GENES TRANSCRIBED BY F
4 PROMOTERS.5R
2.2 IDENTIFICATION OF THE TRANSCRIPTIONAL ACTIVATOR OF THE CF4-DEPENDENT
GENES AND OPERONS.60
2.3 TRANSCRIPTIONAL ANALYSIS OF THE FLGR GENE.62
2.4 FLGR AS THE MASTER REGULATOR OF BASAL BODY AND HOOK GENES.63
2.5 BINDING OF FLGR TO THE FIAB PROMOTER.64
2.6 EFFECT OF FLGR ON FLAA TRANSCRIPTION.66
2.7 EFFECT OF DNA TOPOLOGY ON FLAB TRANSCRIPTION.6'
3. TRANSCRIPTIONAL REGULATION OF CHAPERONE GENES.68
3.1 DETERMINATION OF TRANSCRIPTIONAL START SITES
.6H
3.2 IDENTIFICATION OF THE TRANSCRIPTIONAL REGULATOR OF THE CHAPERONE
GENES.7()
3.3 EFFECT OF HSPR ON CELLULAR LEVELS OF GROEL AND UREASE. ."'2
3.4 DEREPRESSION OF P^, AND P^P PROMOTERS BY OSMOTIC SHOCK.72
3.5 BINDING OF HSPR TO THE P^, PROMOTER.74
3.6 DIFFERENTIAL BINDING OF HSPR TO THE PGYY, PCHP AND P^ PROMOTERS.7 5
F. DISCUSSION.78
1. PATHOGENICITY ISLAND.78
2.
FLAGELLA
.80
3. CHAPERONES.84
G. CONCLUSIONS.87
H. REFERENCES.YY)()
I. ABBREVIATIONS.102
J. CURRICULUM VITAE.104
6 |
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| indexdate | 2024-10-09T18:12:09Z |
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| language | English |
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| physical | 106 Bl. Ill., graph. Darst. |
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| spelling | Spohn, Gunther 1969- Verfasser (DE-588)122383214 aut The transcriptional control of virulence gene expression in Helicobacter pylori von Gunther Spohn 1999 106 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. Sprache. - Würzburg, Univ., Diss., 2000 Helicobacter-pylori-Infektion (DE-588)4206296-2 gnd rswk-swf Virulenz (DE-588)4129834-2 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Helicobacter-pylori-Infektion (DE-588)4206296-2 s Virulenz (DE-588)4129834-2 s Genexpression (DE-588)4020136-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009008667&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Spohn, Gunther 1969- The transcriptional control of virulence gene expression in Helicobacter pylori Helicobacter-pylori-Infektion (DE-588)4206296-2 gnd Virulenz (DE-588)4129834-2 gnd Genexpression (DE-588)4020136-3 gnd |
| subject_GND | (DE-588)4206296-2 (DE-588)4129834-2 (DE-588)4020136-3 (DE-588)4113937-9 |
| title | The transcriptional control of virulence gene expression in Helicobacter pylori |
| title_auth | The transcriptional control of virulence gene expression in Helicobacter pylori |
| title_exact_search | The transcriptional control of virulence gene expression in Helicobacter pylori |
| title_full | The transcriptional control of virulence gene expression in Helicobacter pylori von Gunther Spohn |
| title_fullStr | The transcriptional control of virulence gene expression in Helicobacter pylori von Gunther Spohn |
| title_full_unstemmed | The transcriptional control of virulence gene expression in Helicobacter pylori von Gunther Spohn |
| title_short | The transcriptional control of virulence gene expression in Helicobacter pylori |
| title_sort | the transcriptional control of virulence gene expression in helicobacter pylori |
| topic | Helicobacter-pylori-Infektion (DE-588)4206296-2 gnd Virulenz (DE-588)4129834-2 gnd Genexpression (DE-588)4020136-3 gnd |
| topic_facet | Helicobacter-pylori-Infektion Virulenz Genexpression Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009008667&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT spohngunther thetranscriptionalcontrolofvirulencegeneexpressioninhelicobacterpylori |