A study of antigen presenting cell function in HIV-1 infected patients:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
1999
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Köln, Univ., Diss., 1999 |
| Beschreibung: | 106 S. graph. Darst. : 21 cm |
Internformat
MARC
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| 100 | 1 | |a Schneider, Lars-Alexander |d 1971- |e Verfasser |0 (DE-588)121847772 |4 aut | |
| 245 | 1 | 0 | |a A study of antigen presenting cell function in HIV-1 infected patients |c vorgelegt von Lars-Alexander Schneider |
| 264 | 1 | |c 1999 | |
| 300 | |a 106 S. |b graph. Darst. : 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Köln, Univ., Diss., 1999 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008945759&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-008945759 | |
Datensatz im Suchindex
| _version_ | 1807504867504160768 |
|---|---|
| adam_text |
TABLE
OF
CONTENTS:
1.
INTRODUCTION
.
1
1.1.
BACKGROUND
AND TOPIC
OF
THE
THESIS
.
1
1.2.
CELLULAR
IMMUNOLOGY
AND
HIV-INFECTION
.
3
1.2.1.
PERIPHERAL
BLOOD
MONONUCLEAR
CELLS
.
3
1.2.2.
ANTIGEN
RECOGNITION
AND
CD4
T-CELL
ACTIVATION
.
3
1.2.3.
T-CELL
CLONES
(TLC).
7
1.2.4.
PATHOGENESIS
OF
HIV
INFECTION
.
7
1.2.5.
THE
LOSS
OF
RESPONSE
TO
RECALL
ANTIGENS
IN
AIDS
.
9
2.
MATERIALS
AND
METHODS
.
1
1
2.1.
PATIENTS
AND
CONTROLS
.
11
2.1.1.
THE
HIV-1
INFECTED
PATIENTS
.
11
2.1.2.
THE
CONTROLS.
1
4
2.1.3.
TISSUE
TYPING
OF
PATIENTS
AND
CONTROLS
.
14
2.2.
LABORATORY
TECHNIQUES
.
1
7
2.2.1.
ISOLATION
OF
PBMC
.
17
2.2.2.
CELL
CULTURE
MEDIUM
.
1
7
2.2.3.
PPD/TETANUS
TOXOID.
1
7
2.2.4.
FREEZING
CELLS
.
1
8
2.2.5.
THAWING
CELLS
.
1
8
2.2.6.
PERCOLL
GRADIENTS.
1
8
2.2.7.
ISOLATION
OF
CD4+
T-CELLS
.
1
8
2.2.7.1.
T-CELL
SEPARATION
USING
PANNING
DEPLETION
STRATEGY
.
19
2.2.7.2.
MAGNETIC
CELL
SORTER
(MACS)
PROTOCOL
FOR
POSITIVE
CD4
SELECTION
.
1
9
2.2.7.3.
T-CELL
SEPARATION
WITH
MACS
USING
NEGATIVE
SELECTION
.
20
2.2.8.
MONOCYTE
SEPARATION
USING
MACS
.
21
2.2.9.
FACS
STAINING
PREPARATION
.
21
2.3.
PROLIFERATION
ASSAYS.
2
3
2.3.1.
BULK
RECALL
RESPONSE
CULTURES
.
2
3
2.3.2.
POLYCLONAL
T-CELL
ASSAY
WITH
RECALL
ANTIGENS
.
24
2.3.3.
FUNCTIONAL
ELIMINATION
OF
T-CELLS
IN
PBMC
.
25
2.3.4.
ASSAY
SET-UP
FOR
EVALUATION
OF
CD4
T-CELL
FUNCTION
.
25
3.
RESULTS.
.
2
7
3.1.
EVALUATION
OF
METHODOLOGIES
.
27
3.1.1.
CD4
T-CELL
SEPARATION
METHODOLOGIES
.
27
3.1.1.1.
YIELD
AND
PURITY
.
.
28
3.1.1.2.
PHA
RESPONSES
OF
PURIFIED
T-CELLS
.
29
3.1.1.3.
RESPONSES
TO
RECALL
ANTIGENS
IN
THE
PRESENCE
OF
ADDED
APC
.
30
3.1.1.4
DISCUSSION
OF
T-CELL
ISOLATION
PROTOCOLS
.
30
3.1.2.
APC
PURIFICATION
STRATEGIES
.
31
3.1.3.
COMPARISON
OF
YIELD
AND
PURITY
FROM
HIV+
AND
HIV
PBMC
.
32
3.2.
COMPARISON
OF
HIV+
AND
HIV
APC
FUNCTION
.
36
3.2.1.
BULK
CULTURES
AND
RECALL
RESPONSES
.
36
3.2.2.
RESPONSES
OF
+VE
SELECTED
T-CELLS
TO
ALLOANTIGENS
EXPRESSED
ON
HIV+
AND
HIV
APC
.
39
3.2.3.
RESPONSES
OF
+VE
SELECTED
T-CELLS
TO
PPD/TET.
PRESENTED
BY
HIV+/HIV-APC
.
39
3.2.4.
RESPONSES
OF
+VE
SELECTED
T-CELLS
TO
PPD/TET.
PRESENTED
BY
PURIFIED
ME/DC
FROM
HIV+
AND
HIV
DONORS
.
48
3.2.5.
RESPONSES
OF
-VE
SELECTED
T-CELLS
TO
RECALL
AND
ALLOANTIGENS
ON
HIV+/HIV-APC
.51
3.2.6.
COMPARISON
OF
BULK
RESPONSES
AND
APC
FUNCTION
OF
HIV+
DONORS
.
53
4.
DISCUSSION
.
5
5
5.
SUMMARY
.
6
3
6.
ZUSAMMENFASSUNG
DER
ARBEIT
.
6
5
7.
REFERENCES
.
6
9
8.
APPENDIX
.
8
0
9.
CURRICULUM
VITAE
.
105 |
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| author | Schneider, Lars-Alexander 1971- |
| author_GND | (DE-588)121847772 |
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| building | Verbundindex |
| bvnumber | BV013130900 |
| ctrlnum | (OCoLC)1071510090 (DE-599)BVBBV013130900 |
| discipline | Medizin |
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| indexdate | 2024-08-16T01:16:08Z |
| institution | BVB |
| language | English |
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| physical | 106 S. graph. Darst. : 21 cm |
| publishDate | 1999 |
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| spelling | Schneider, Lars-Alexander 1971- Verfasser (DE-588)121847772 aut A study of antigen presenting cell function in HIV-1 infected patients vorgelegt von Lars-Alexander Schneider 1999 106 S. graph. Darst. : 21 cm txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 1999 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008945759&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Schneider, Lars-Alexander 1971- A study of antigen presenting cell function in HIV-1 infected patients |
| subject_GND | (DE-588)4113937-9 |
| title | A study of antigen presenting cell function in HIV-1 infected patients |
| title_auth | A study of antigen presenting cell function in HIV-1 infected patients |
| title_exact_search | A study of antigen presenting cell function in HIV-1 infected patients |
| title_full | A study of antigen presenting cell function in HIV-1 infected patients vorgelegt von Lars-Alexander Schneider |
| title_fullStr | A study of antigen presenting cell function in HIV-1 infected patients vorgelegt von Lars-Alexander Schneider |
| title_full_unstemmed | A study of antigen presenting cell function in HIV-1 infected patients vorgelegt von Lars-Alexander Schneider |
| title_short | A study of antigen presenting cell function in HIV-1 infected patients |
| title_sort | a study of antigen presenting cell function in hiv 1 infected patients |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008945759&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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