Mutational and transcriptional analysis of a hydrogenase gene cluster in the cyanobacterium Synechocystis sp. PCC 6803:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
1999
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Marburg, Univ., Diss., 1999 |
| Beschreibung: | 129 S. Ill., graph. Darst. : 21 cm |
Internformat
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| 100 | 0 | |a Saranya Phunpruch |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Mutational and transcriptional analysis of a hydrogenase gene cluster in the cyanobacterium Synechocystis sp. PCC 6803 |c vorgelegt von Saranya Phunpruch |
| 264 | 1 | |c 1999 | |
| 300 | |a 129 S. |b Ill., graph. Darst. : 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Marburg, Univ., Diss., 1999 | ||
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| 650 | 0 | 7 | |a Gencluster |0 (DE-588)4355303-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Hydrogenasen |0 (DE-588)4160910-4 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
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| 689 | 0 | 1 | |a Hydrogenasen |0 (DE-588)4160910-4 |D s |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-008915723 | |
Datensatz im Suchindex
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| adam_text |
CONTENTS
1.
INTRODUCTION
1
1.1.
CLASSIFICATION
OF
HYDROGENASES
1
1.2.
NIFE-HYDROGENASES
3
1.3.
MEMBRANE-BOUND
HYDROGENASES
(MBH)
4
1.4.
SOLUBLE
HYDROGENASES
(SH)
5
1.5.
IRON-ONLY
HYDROGENASES
6
1.6.
HYDROGENASES
OF
CYANOBACTERIA
7
1.7.
PHYSIOLOGY
OF
HZ
METABOLISM
9
1.8.
MUTATIONAL
ANALYSIS
OFHYDROGENASE
AND
INVOLVING
GENES
11
1.9.
HYDROGENASE
OF
SYNECHOCYSTIS
SP.
PCC
6803
12
2.
AIMS
OF
THE
PRESENT
STUDY
15
3.
MATERIALS
AND
METHODS
16
3.1.
MATERIALS
16
3.1.1.
BACTERIAL
STRAINS
16
3.1.2.
PLASMIDS
16
3.1.3.
RESTRICTION
ENZYMES
16
3.1.4.
OTHER
ENZYMES
16
3.1.5.
ANTIBIOTICS
17
3.1.6.
KITS
17
3.1.7.
MOLECULAR
WEIGHT
MARKERS
17
3.1.8.
PROTEIN
MARKERS
17
3.1.9.
RADIOACTIVES
18
3.1.10.
MEMBRANE
&
PAPERS
&
FILMS
18
3.1.11.
CHEMICALS
18
3.1.12.
EQUIPMENT
19
3.1.13.
MISCELLANEOUS
19
3.2.
METHODS
20
3.2.1.
BACTERIAL
STRAINS
AND
GROWTH
CONDITIONS
20
3.2.1.1.
SYNECHOCYSTIS
SP.
PCC
6803
20
3.2.1.2.
E.
COLI
21
3.2.2.
THE
QUANTITATIVE
DETERMINATION
OF
DIFFERENT
PARAMETERS
22
3.2.2.1.
CELL
TURBIDITY
MEASUREMENT
22
3.2.2.2.
CELL
NUMBER
MEASUREMENT
22
3
2.2.3.
IN
VITRO
ABSORBANCE
MEASUREMENT
OF
SYNECHOCYSTIS
23
3.2.2.4.
DETERMINATION
OF
NUCLEIC
ACIDS
CONCENTRATION
23
3.2.2.5.
CHLOROPHYLL
MEASUREMENT
23
3.2.2.6.
HYDROGENASE
ACTIVITY
MEASUREMENT
24
3.2.2.7.
PROTEIN
DETERMINATION
25
3.2.3.
MOLECULAR
BIOLOGY
METHODS
25
3.2.3.1.
DNA
AND
RNA
ISOLATION
FROM
SYNECHOCYSTIS
25
3.2.3.2.
PLASMID
DNA
PREPARATION
27
3.2.3.3.
RESTRICTION
ENZYME
DIGESTION
29
3.2.3.4.
ALKALINE
PHOSPHATASE
DIGESTION
29
3.2.3.5.
LIGATION
29
3.2.3.6.
TRANSFORMATION
30
3.2.3.7.
AGAROSE
GEL
ELECTROPHORESIS
OF
THE
NUCLEIC
ACIDS
31
3.2.3.8.
BINDING
AND
RECOVERY
OF
DNA
FROM
AGAROSE
GELS
USING
NA-45
DEAE
MEMBRANE
33
3.2.3.9.
BLOTTING
METHODS
34
3.2.3.10.
POLYMERASE
CHAIN
REACTION
(PCR)-MEDIATED
DNA
AMPLIFICATION
39
3.2.3.11.
REVERSE
TRANSCRIPTION-POLYMERASE
CHAIN
REACTION
(RT-PCR)
40
3.2.3.12.
CLONING
OF
PCR-FRAGMENT
42
3.2.3.13.
DNA
SEQUENCING
43
3.2.3.14.
SCREENING
A
X-ZAP
GENOMIC
LIBRARY
OF
SYNECHOCYSTIS
SP.
PCC
6803
47
3.2.3.15.
PRIMER
EXTENSION
49
3.2.4.
WESTERN
BLOTTING
50
4.
RESULTS
AND
DISCUSSION
54
4.1.
CHARACTERIZATION
OF
HYDROGENASE
ACTIVITY
IN
SYNECHOCYSTIS
SP.
PCC
6803
54
4.1.1.
HYDROGENASE
ACTIVITY
OF
SYNECHOCYSTIS
SP.
PCC
6803
54
4.1.2.
HYDROGENASE
ACTIVITY
OF
SYNECHOCYSTIS
SP.
PCC
6803
DURING
ANAEROBIC
ADAPTATION
55
4.2
SEARCH
FOR
OTHER
HYDROGENASES
IN
SYNECHOCYSTIS
BY
SCREENING
THE
X-ZAP
GENOMIC
LIBRARY
56
4.3.
MUTAGENESIS
OF
HOX
GENES
IN
SYNECHOCYSTIS
SP.
PCC
6803
59
4.3.1.
CONSTRUCTION
OF
VECTORS
FOR
GENERATION
OF
MUTANTS
59
4.3.2.
INVESTIGATION
OF
THE
MUTANTS
BY
PCR
AND
SOUTHERN
HYBRIDIZATION
64
4.4.
ANALYSIS
OF
THE
HOX
MUTANTS
74
4.4.1.
THE
HYDROGENASE
ACTIVITY
OF
MUTANTS
4.4.2
TRANSCRIPTIONAL
LEVEL
ANALYSIS
OF
HOXH
IN
SHOXE,
SHOXF,
74
SHOXU
AND
SHOXH
MUTANTS
76
4.4.3.
WESTERN
BLOT
ANALYSIS
77
4.4.4.
GROWTH
CHARACTERISTICS
4.4.5.
EFFECT
OF
DIFFERENT
LIGHT
INTENSITIES
ON
GROWTH
CHARACTERISTICS
78
OF
WILD-TYPE
AND
EHOXH
MUTANT
82
4.4.6.
TRANSCRIPTION
OF
PHOTOSYNTHETIC
GENES
4.4.7.
DISCUSSION
OF
THE
PARTICIPATION
OF
HYDROGEN
METABOLISM
IN
88
PHOTOSYNTHETIC
AND
RESPIRATORY
ELECTRON
FLOW
91
4.5.
ANALYSIS
OF
NDHD
MUTANTS
94
4.5
1
GROWTH
ANALYSIS
OF
SNDHD
MUTANT
94
4.5.2.
TRANSCRIPTION
OF
NDHDX
AND
NDHDL
GENES
96
4.6.
CHARACTERIZATION
OF
THE
TRANSCRIPTION
OF
HOX
GENES
101
4.6.1.
TRANSCRIPTIONAL
ANALYSIS
OF
HOX
GENES
4.6.2.
DETERMINATION
OF
TRANSCRIPTION
INITIATION
SITE
OF
HOX
OPERON
IN
101
SYNECHOCYSTIS
4.6.3.
DETERMINATION
OF
TRANSCRIPTION
TERMINATION
SITE
OF
HOX
OPERON
IN
101
SYNECHOCYSTIS
108
5.
SUMMARY
6.
ZUSAMMENFASSUNG
7.
FUTURE
PERSPECTIVES
8.
REFERENCES
9.
ACKNOWLEDGEMENTS
109
112
115
116
129 |
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| author | Saranya Phunpruch |
| author_facet | Saranya Phunpruch |
| author_role | aut |
| author_sort | Saranya Phunpruch |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV013088883 |
| illustrated | Illustrated |
| indexdate | 2024-08-19T00:17:52Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008915723 |
| oclc_num | 247049343 |
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| physical | 129 S. Ill., graph. Darst. : 21 cm |
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| spelling | Saranya Phunpruch Verfasser aut Mutational and transcriptional analysis of a hydrogenase gene cluster in the cyanobacterium Synechocystis sp. PCC 6803 vorgelegt von Saranya Phunpruch 1999 129 S. Ill., graph. Darst. : 21 cm txt rdacontent n rdamedia nc rdacarrier Marburg, Univ., Diss., 1999 Synechocystis (DE-588)4366130-0 gnd rswk-swf Gencluster (DE-588)4355303-5 gnd rswk-swf Hydrogenasen (DE-588)4160910-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Synechocystis (DE-588)4366130-0 s Hydrogenasen (DE-588)4160910-4 s Gencluster (DE-588)4355303-5 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008915723&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Saranya Phunpruch Mutational and transcriptional analysis of a hydrogenase gene cluster in the cyanobacterium Synechocystis sp. PCC 6803 Synechocystis (DE-588)4366130-0 gnd Gencluster (DE-588)4355303-5 gnd Hydrogenasen (DE-588)4160910-4 gnd |
| subject_GND | (DE-588)4366130-0 (DE-588)4355303-5 (DE-588)4160910-4 (DE-588)4113937-9 |
| title | Mutational and transcriptional analysis of a hydrogenase gene cluster in the cyanobacterium Synechocystis sp. PCC 6803 |
| title_auth | Mutational and transcriptional analysis of a hydrogenase gene cluster in the cyanobacterium Synechocystis sp. PCC 6803 |
| title_exact_search | Mutational and transcriptional analysis of a hydrogenase gene cluster in the cyanobacterium Synechocystis sp. PCC 6803 |
| title_full | Mutational and transcriptional analysis of a hydrogenase gene cluster in the cyanobacterium Synechocystis sp. PCC 6803 vorgelegt von Saranya Phunpruch |
| title_fullStr | Mutational and transcriptional analysis of a hydrogenase gene cluster in the cyanobacterium Synechocystis sp. PCC 6803 vorgelegt von Saranya Phunpruch |
| title_full_unstemmed | Mutational and transcriptional analysis of a hydrogenase gene cluster in the cyanobacterium Synechocystis sp. PCC 6803 vorgelegt von Saranya Phunpruch |
| title_short | Mutational and transcriptional analysis of a hydrogenase gene cluster in the cyanobacterium Synechocystis sp. PCC 6803 |
| title_sort | mutational and transcriptional analysis of a hydrogenase gene cluster in the cyanobacterium synechocystis sp pcc 6803 |
| topic | Synechocystis (DE-588)4366130-0 gnd Gencluster (DE-588)4355303-5 gnd Hydrogenasen (DE-588)4160910-4 gnd |
| topic_facet | Synechocystis Gencluster Hydrogenasen Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008915723&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT saranyaphunpruch mutationalandtranscriptionalanalysisofahydrogenasegeneclusterinthecyanobacteriumsynechocystissppcc6803 |