Approach to the biological control of Macrophomina phaseolina (Tassi) Goid, causal agent of charcoal rot of cowpea (Vigna unguiculata (L.) Walp), and development of serological methods for its detection:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Göttingen
Cuvillier
1999
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| Ausgabe: | 1. Aufl. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zugl.: Göttingen, Univ., Diss., 1999 |
| Beschreibung: | V, 139 S. Ill., graph. Darst. : 21 cm |
| ISBN: | 389712730X |
Internformat
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Datensatz im Suchindex
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| adam_text | Titel: Approach to the biological control of Macrophomina phaseolina (Tassi) Goid, causal agent of charcoal
Autor: Afouda, Leonard A. C.
Jahr: 1999
Contents Pages
1 Introduction 1
2 Material and methods 13
2.1 Chemicals 13
2.2 Culture media 14
2.3 Origin of samples IS
2.4 Cultivation of cowpea plants 16
2.5 Culture of microorganisms and their storage 16
2.6 Isolation of M. phaseolina 17
2.6.1 Isolation from soil 17
2.6.2 Isolation from seeds 17
2.6.3 Isolation from plant material 18
2.7 Pathogenicity tests 18
2.7.1 Methods for soil inoculation 18
2.7.2 Methods for seed inoculation 19
2.8 Poly aery lamide gel elecrrophoresis (PAGE) 19
2.8.1 Determination of the protein concentration 19
2.8.2 Sample preparation 20
2.8.3 Preparation of linear gradient gels 21
2.8.4 Silver staining 23
2.9 Enzymatic methods for the detection of M. phaseolina infections in plant
tissues 24
2.10 Immunological methods for the detection and quantification of
M. phaseolina in plant tissue and seeds 25
2.10.1 Antiserum production 25
2.10.1.1 Preparation of the antigen 25
2.10.1.2 Immunisation of rabbits 26
2.10.2 Purification of the immunoglobulin 26
2.10.3 Biotinylation of the antibody 27
2.10.4 Double antibody enzyme-linked immunosorbent assay (DAS-EL1SA) 28
2.10.5 Tests of the antibodies for specificity and sensitivity 30
2.10.6 Test of seeds for the level of infection 30
2.11 Biological control of M. phaseolina with microbial antagonists 31
2.11.1 Isolation of antagonists 31
2.11.2 In vitro tests 32
2.11.3 Test of the antagonists on antibacterial effects 32
2.11.4 Greenhouse experiments 32
2.11.4.1 Seed treatment with bacterial antagonists 32
2.11.5 Taxonomy of the antagonists 33
2.11.5.1 Microscopic observation 33
2.11.5.2 Test for heat resistance 33
2.11.5.3 Aminopeptidase test 3 3
2.11.5.4 KOH test 34
2.11.5.5 API test 34
2.11.6 Rhizosphere competence of the antagonists 36
2.11.6.1 Production of rifampicin and streptomycin-resistant spontaneous
mutants of bacilli antagonists 36
2.11.6.2 Studies on the rhizosphere competence of the antagonist Al 1 37
2.12 Screening ofcowpea varieties for resistance to M. phaseolina 37
3 Results 38
3.1 Disease surveys in Niger and observation of macroscopic and
microscopic symptoms caused by M. phaseolina 38
3.2 Isolates ofM. phaseolina 42
33 Characterisation of isolates of M. phaseolina 43
3.3.1 Temperature requirements of M. phaseolina 43
3.3.2 Growth of M. phaseolina on common cultural media 44
3.3.3 Host specificity of isolates of M. phaseolina 45
3.3.4 Polyacrylamide gel electrophoresis (PAGE) for the characterisation of
M. phaseolina isolates 46
3.4 Optimisation of the test system 48
3.4.1 Influence of temperature and water regime on the pathogenicity of
M. phaseolina 48
3.4.2 Influence of soil composition on infection of plants with M. phaseolina 49
3.4.3 Influence of inoculum concentration 49
3.5 Enzymatic methods for the detection of M. phaseolina 51
3.6 Immuoological methods for the detection and quantification of
M. phaseolina in plant tissue and in seeds 52
3.6.1 Optimisation of the detection system 53
3.6.2 Sensitivity of antibody 848/3 55
3.6.3 Specificity of the antibodies 56
3.6.4 Distribution of M phaseolina in the infected plant 58
3.6.5 Latent occurrence of M. phaseolina 60
3.6.6 Detection of M. phaseolina in cowpea seeds 62
3.7 Biological control of M. phaseolina with microbial antagonists 63
3.7.1 Isolates of antagonists 63
3.7.2 In vitro tests 66
3.7.3 Test of the antagonist Al 1 on antibaterial effects 71
3.7.4 Test of the antagonists adplanta 71
3.7.5 Rhizosphere competence of All 76
rv______________________________________________________
3.7.6 Taxonomy of the antagonists 79
3.7.6.1 Physiological properties of the antagonists: heat resistance, amino peptidase
and KOH tests 79
3.7.6.2 Identification of the antagonists with the API system 80
3.8 Test of cowpea genotypes for resistance to M. phaseolina 82
4 Discussion 87
5 Summary 119
6 References 122
7 Annex
|
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| bvnumber | BV013005853 |
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| illustrated | Illustrated |
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| institution | BVB |
| isbn | 389712730X |
| language | English |
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| physical | V, 139 S. Ill., graph. Darst. : 21 cm |
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| publishDateSearch | 1999 |
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| spelling | Afouda, Leonard A. C. Verfasser aut Approach to the biological control of Macrophomina phaseolina (Tassi) Goid, causal agent of charcoal rot of cowpea (Vigna unguiculata (L.) Walp), and development of serological methods for its detection by Leonard A. C. Afouda 1. Aufl. Göttingen Cuvillier 1999 V, 139 S. Ill., graph. Darst. : 21 cm txt rdacontent n rdamedia nc rdacarrier Zugl.: Göttingen, Univ., Diss., 1999 Macrophomina phaseolina (DE-588)4584976-6 gnd rswk-swf Biologischer Pflanzenschutz (DE-588)4112773-0 gnd rswk-swf Augenbohne (DE-588)4209340-5 gnd rswk-swf Niger Süd (DE-588)4379374-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Niger Süd (DE-588)4379374-5 g Augenbohne (DE-588)4209340-5 s Biologischer Pflanzenschutz (DE-588)4112773-0 s Macrophomina phaseolina (DE-588)4584976-6 s DE-604 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008864202&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Afouda, Leonard A. C. Approach to the biological control of Macrophomina phaseolina (Tassi) Goid, causal agent of charcoal rot of cowpea (Vigna unguiculata (L.) Walp), and development of serological methods for its detection Macrophomina phaseolina (DE-588)4584976-6 gnd Biologischer Pflanzenschutz (DE-588)4112773-0 gnd Augenbohne (DE-588)4209340-5 gnd |
| subject_GND | (DE-588)4584976-6 (DE-588)4112773-0 (DE-588)4209340-5 (DE-588)4379374-5 (DE-588)4113937-9 |
| title | Approach to the biological control of Macrophomina phaseolina (Tassi) Goid, causal agent of charcoal rot of cowpea (Vigna unguiculata (L.) Walp), and development of serological methods for its detection |
| title_auth | Approach to the biological control of Macrophomina phaseolina (Tassi) Goid, causal agent of charcoal rot of cowpea (Vigna unguiculata (L.) Walp), and development of serological methods for its detection |
| title_exact_search | Approach to the biological control of Macrophomina phaseolina (Tassi) Goid, causal agent of charcoal rot of cowpea (Vigna unguiculata (L.) Walp), and development of serological methods for its detection |
| title_full | Approach to the biological control of Macrophomina phaseolina (Tassi) Goid, causal agent of charcoal rot of cowpea (Vigna unguiculata (L.) Walp), and development of serological methods for its detection by Leonard A. C. Afouda |
| title_fullStr | Approach to the biological control of Macrophomina phaseolina (Tassi) Goid, causal agent of charcoal rot of cowpea (Vigna unguiculata (L.) Walp), and development of serological methods for its detection by Leonard A. C. Afouda |
| title_full_unstemmed | Approach to the biological control of Macrophomina phaseolina (Tassi) Goid, causal agent of charcoal rot of cowpea (Vigna unguiculata (L.) Walp), and development of serological methods for its detection by Leonard A. C. Afouda |
| title_short | Approach to the biological control of Macrophomina phaseolina (Tassi) Goid, causal agent of charcoal rot of cowpea (Vigna unguiculata (L.) Walp), and development of serological methods for its detection |
| title_sort | approach to the biological control of macrophomina phaseolina tassi goid causal agent of charcoal rot of cowpea vigna unguiculata l walp and development of serological methods for its detection |
| topic | Macrophomina phaseolina (DE-588)4584976-6 gnd Biologischer Pflanzenschutz (DE-588)4112773-0 gnd Augenbohne (DE-588)4209340-5 gnd |
| topic_facet | Macrophomina phaseolina Biologischer Pflanzenschutz Augenbohne Niger Süd Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008864202&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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