Cofilin: a novel link between T cell activation and the rearrangement of the actin cytoskeleton
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| Main Author: | |
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| Format: | Book |
| Language: | English |
| Published: |
1999
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Zsfassung in dt. Sprache. - Würzburg, Univ., Diss., 2000 |
| Physical Description: | IV, 105 Bl. Ill., graph. Darst. |
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| 100 | 1 | |a Lee, Kyeong-Hee |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Cofilin |b a novel link between T cell activation and the rearrangement of the actin cytoskeleton |c von Kyeong-Hee Lee |
| 264 | 1 | |c 1999 | |
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Record in the Search Index
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| adam_text |
CONTENTS
CONTENTS
1. INTRODUCTION. 1
1.1 T CELL ACTIVATION.1
1.2 ACTIN CYTOSKELETON.7
1.3 COFILIN. 11
1.4 AIM OF THE STUDY.1.16
2. MATERIALS & METHODS. 17
2.1. MATERIAL. 17
2.1.1 GENERAL CHEMICALS AND MATERIALS.17
2.1.2 CELL CULTURE MATERIALS.19
2.1.3 INHIBITORS. 19
2.1.4 RADIOCHEMICALS.19
2.1.5 PEPTIDES.20
2.1.6 TECHNICAL EQUIPMENT.20
2.1.7 ANTIBODIES.21
2.1.8 CELLS.23
I
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/959864792
.CONTENTS.
2.2 METHODS. 24
2.2.1 STIMULATION OF CELLS. 24
2 .2.2 PREPARATION OF CYTOPLASMIC AND ACTIN CYTOSKELETAL FRACTIONS.26
2.2.3 SDS-POLYACRYLAMIDE GEL-ELECTROPHORESIS (SDS-PAGE).26
2.2.4 NON-EQUILIBRIUM PH GRADIENT ELECTROPHORESIS (NEPHGE).28
2.2.5 WESTERN-BLOTTING. 29
2.2.6 COUPLING OF PEPTIDES TO PENETRATIN AND PURIFICATION OF THE
CONJUGATES.30
2.2.7 ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA).31
2.2.8 CD2 RECEPTOR CAP FORMATION AND IMMUNOFLUORESCENCE MICROSCOPY.33
2.2.9 SURFACE AND INTRACELLULAR STAINING FOR FLOW CYTOMETRY. 34
2.2.10 PROLIFERATION ASSAY.35
2.2.11 DETERMINATION OF APOPTOSIS.36
3. RESULTS. 39
3.1 ACTIVATION OF HUMAN PERIPHERAL BLOOD T LYMPHOCYTES INDUCES
REVERSIBLE
ASSOCIATION OF COFILIN WITH THE ACTIN CYTOSKELETON.39
3.2 IN ACTIVATED T LYMPHOCYTES THE DEPHOSPHORYLATED FORM OF COFILIN
ASSOCIATES WITH
THE ACTIN CYTOSKELETON.41
3.3 COFILIN CO-IMMUNOPRECIPITATES WITH CYTOSKELETAL ACTIN IN ACTIVATED
T LYMPHOCYTES.42
3.4 THE PI3-KINASE INHIBITOR WORTMANNIN PREVENTS DEPHOSPHORYLATION OF
COFILIN AND ITS
ASSOCIATION WITH THE ACTIN CYTOSKELETON IN PERIPHERAL BLOOD T
LYMPHOCYTES BUT NOT
IN JURKAT T LYMPHOMA CELLS.44
3.5 STIMULATION WITH PMA DOES NOT INFLUENCE THE ASSOCIATION OF COFILIN
WITH THE
ACTIN CYTOSKELETON.46
II
CONTENTS
3.6 WORTMANNIN DOES NOT INHIBIT THE CONSTITUTIVE ASSOCIATION OF COFILIN
WITH THE
ACTIN CYTOSKELETON IN JURKAT T LYMPHOMA CELLS.48
3.7 THE ACTIVATION INDUCED ASSOCIATION OF COFILIN WITH THE ACTIN
CYTOSKELETON IS
BLOCKED BY COFILIN DERIVED PEPTIDES.49
3.8 PENETRATIN COUPLED PEPTIDES EFFICIENTLY INTERNALIZE INTO LIVING
CELLS.51
3.9 THE ACTIVATION INDUCED ASSOCIATION OF COFILIN WITH THE ACTIN
CYTOSKELETON
IN VIVO
IS BLOCKED BY CELL PERMEABLE COFILIN DERIVED PEPTIDES.52
3.10 COFILIN IS INVOLVED IN THE PROCESS OF THE RECEPTOR CAP FORMATION.54
3.11 THE INTERACTION OF COFILIN WITH THE ACTIN CYTOSKELETON IS REQUIRED
FOR T CELL
ACTIVATION.58
3.12 THE COFILIN DERIVED PEPTIDES INHIBIT CD2-MEDIATED ACTIVATION
INDUCED CELL DEATH
(ACID).--.64
3.13 COFILIN DERIVED PEPTIDES DO NOT INFLUENCE CD95-INDUCED APOPTOSIS.66
3.14. APOPTOSIS INDUCED VIA CD2 IS INHIBITED BY SOLUBLE ANTI-CD95 MAB.68
3.15 COFILIN DERIVED PEPTIDES INHIBIT THE EXPRESSION OF CD95 LIGAND.70
4. DISCUSSION.72
4.1 REVERSIBLE ASSOCIATION OF COFILIN WITH THE ACTIN CYTOSKELETON
FOLLOWING ACTIVATION
OF HUMAN PERIPHERAL BLOOD T LYMPHOCYTES.72
4.2 INVOLVEMENT OF PI3-KINASE ACTIVITY IN THE COFILIN SIGNALING
PATHWAY.74
4.3 BLOCKADE OF THE ACTIVATION INDUCED INTERACTION OF COFILIN WITH THE
ACTIN CYTOSKELETON
BY COFILIN DERIVED PEPTIDES IN VIVO.75
4.4 INVOLVEMENT OF COFILIN IN THE PROCESS OF RECEPTOR CAP FORMATION.76
4.5 THE INTERACTION OF COFILIN WITH THE ACTIN CYTOSKELETON IS AN
IMPORTANT STEP FOR
T CELL ACTIVATION.78
IN
.CONTENTS
4.6 INVOLVEMENT OF COFILIN IN CD2-MEDIATED ACTIVATION INDUCED PROGRAMMED
CELL
DEATH.80
5. SUMMARY.
6. REFERENCES.
86
7. ABBREVIATIONS.
104
IV |
| any_adam_object | 1 |
| author | Lee, Kyeong-Hee |
| author_facet | Lee, Kyeong-Hee |
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| bvnumber | BV012999185 |
| ctrlnum | (OCoLC)76185680 (DE-599)BVBBV012999185 |
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| indexdate | 2024-10-09T18:12:10Z |
| institution | BVB |
| language | English |
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| physical | IV, 105 Bl. Ill., graph. Darst. |
| publishDate | 1999 |
| publishDateSearch | 1999 |
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| spelling | Lee, Kyeong-Hee Verfasser aut Cofilin a novel link between T cell activation and the rearrangement of the actin cytoskeleton von Kyeong-Hee Lee 1999 IV, 105 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. Sprache. - Würzburg, Univ., Diss., 2000 Lymphozytentransformation (DE-588)4168382-1 gnd rswk-swf Actin (DE-588)4201876-6 gnd rswk-swf Aktivierung Physiologie (DE-588)4243777-5 gnd rswk-swf T-Lymphozyt (DE-588)4127387-4 gnd rswk-swf Cofilin (DE-588)4605260-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content T-Lymphozyt (DE-588)4127387-4 s Aktivierung Physiologie (DE-588)4243777-5 s Actin (DE-588)4201876-6 s DE-604 Cofilin (DE-588)4605260-4 s Lymphozytentransformation (DE-588)4168382-1 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008858566&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Lee, Kyeong-Hee Cofilin a novel link between T cell activation and the rearrangement of the actin cytoskeleton Lymphozytentransformation (DE-588)4168382-1 gnd Actin (DE-588)4201876-6 gnd Aktivierung Physiologie (DE-588)4243777-5 gnd T-Lymphozyt (DE-588)4127387-4 gnd Cofilin (DE-588)4605260-4 gnd |
| subject_GND | (DE-588)4168382-1 (DE-588)4201876-6 (DE-588)4243777-5 (DE-588)4127387-4 (DE-588)4605260-4 (DE-588)4113937-9 |
| title | Cofilin a novel link between T cell activation and the rearrangement of the actin cytoskeleton |
| title_auth | Cofilin a novel link between T cell activation and the rearrangement of the actin cytoskeleton |
| title_exact_search | Cofilin a novel link between T cell activation and the rearrangement of the actin cytoskeleton |
| title_full | Cofilin a novel link between T cell activation and the rearrangement of the actin cytoskeleton von Kyeong-Hee Lee |
| title_fullStr | Cofilin a novel link between T cell activation and the rearrangement of the actin cytoskeleton von Kyeong-Hee Lee |
| title_full_unstemmed | Cofilin a novel link between T cell activation and the rearrangement of the actin cytoskeleton von Kyeong-Hee Lee |
| title_short | Cofilin |
| title_sort | cofilin a novel link between t cell activation and the rearrangement of the actin cytoskeleton |
| title_sub | a novel link between T cell activation and the rearrangement of the actin cytoskeleton |
| topic | Lymphozytentransformation (DE-588)4168382-1 gnd Actin (DE-588)4201876-6 gnd Aktivierung Physiologie (DE-588)4243777-5 gnd T-Lymphozyt (DE-588)4127387-4 gnd Cofilin (DE-588)4605260-4 gnd |
| topic_facet | Lymphozytentransformation Actin Aktivierung Physiologie T-Lymphozyt Cofilin Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008858566&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT leekyeonghee cofilinanovellinkbetweentcellactivationandtherearrangementoftheactincytoskeleton |