Handbook of laboratory animal bacteriology:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Boca Raton [u.a.]
CRC Press
2000
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 255 S. Ill., graph. Darst. |
| ISBN: | 0849329132 9780849329135 |
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| 245 | 1 | 0 | |a Handbook of laboratory animal bacteriology |c Axel Kornerup Hansen |
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| 650 | 7 | |a Bacteriology |2 cabt | |
| 650 | 4 | |a Animaux de laboratoire - Maladies - Diagnostic | |
| 650 | 4 | |a Animaux de laboratoire - Santé | |
| 650 | 4 | |a Bactériologie vétérinaire - Technique | |
| 650 | 4 | |a Animal Diseases |x microbiology | |
| 650 | 4 | |a Bacterial Infections |x veterinary | |
| 650 | 4 | |a Laboratory Techniques and Procedures | |
| 650 | 4 | |a Laboratory animals |x Diseases |x Diagnosis | |
| 650 | 4 | |a Laboratory animals |x Health | |
| 650 | 4 | |a Veterinary bacteriology |x Technique | |
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Record in the Search Index
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|---|---|
| adam_text | HANDBOOK of
LABORATORY ANIMAL
BACTERIOLOGY
G
Axel Kornerup Hansen
CRC Press
Boca Raton London New York Washington, D C
Contents
1 Strategies for sampling animals
for bacteriological examination 1
1 1 Bacterial interference with research 1
1 2 Bacteriological examination of healthy animals 5
121 The aim of examining healthy animals 5
122 Sampling strategies 6
1221 Random sampling 6
1222 Calculation of the sample size 7
1223 Sampling frequency 9
1224 Defining the microbiological entity 10
123 Immunosuppression of animals prior to sampling
(stress testing) 10
124 Screening vs profiling 11
1 3 Bacteriological examination of diseased animals 11
References Q 12
2 Sampling procedures 15
2 1 Planning the work 15
2 2 Euthanasia , 15
2 3 Blood sampling - 18-
2 4 Instruments and sterilizing procedures during sampling 20
2 5 Opening and inspecting the carcass 21
2 6 Sampling from various organs 21
References 46
3i Cultivation methods 47
3 1 The choice of media 47
3 2 Incubation of media 49
3 3 Isolation of bacteria 51
References 54
4 Identification of bacteria 55
4 1 Initial characterization of the isolates 55
4 2 Conclusive identification 56
4 3 Specific techniques used for identification of bacteria 60
431 Gram-stainability tests 60
432 Other methods used for describing
the shape of bacteria 60
433 Motility tests 62
434 Test for aerobic and anaerobic growth 62
435 Catalasetest 63
436 Cytochrome oxidase test 64
437 Acid-fast or spore staining 64
438 Carbohydrate fermentation and utilization assays 64
439 Disc methods 65
4 3 10 Other assays 68
4 3 11 Commercial test kits 68
References 71
5 Immunological methods 75
5 1 Agglutination 76
5 2 Immunofluorescence techniques 77
521 Diagnosing the presence of bacteria in a sample 77
522 The immunofluorescence assay 81
5 3 Immunoenzymatic staining 82
5 4 Enzyme-linked immunosorbent assay 86
541 Principles 86
542 The microtiter plates 87
543 The antigen 93
544 Antibodies, enzymes/and substrates 93
545 Coating the wells 94
546 Blocking the wells 94
547 Performing the assay 95P
548 Control sera 95
549- Interpretation of the OD-value 97
5 5 Immunoblotting 98
References 99
6 Molecular biological methods 101
6 1 Detection by molecular probes 105
611 Solid-phase hybridization 105
612 Solution-phase hybridization 105
6 2 Amplification of nucleic acids 105
621 Principle 105
622 The polymerase enzyme 108
623 Reverse transcriptase RNA PCR 108
624 The template sequence and the primers 109
625 Preparation of the sample 109
626 Detection of the PCR product 109
627 Contamination of the amplification process 110
6 3 Restriction analysis of chromosomal DNA 110
References I l l
7 Gram-positive cocci 113
7 1 Micrococcaceae 114
711 Micrococcus 114
712 Staphylococcus 114
7121 Characteristics of infection 114
7122 Characteristics of the agent 116
71221 Morphology 116
71222 Cultivation 117
71223 Isolation sites 118
71224 Differentiation and identification 118
7 2 Streptococcaceae 118
721 Streptococcus 118
7211 Characteristics of the infection 118
72111 Epidemiology 118
72112 Clinical disease and pathology 120
72113 Interference with research 121
7212 Characteristics of the agent 121
72121 Morphology 121
72122 Media 121
72123 Sampling sites 121
72124 Immunological identification
of isolatesO 121
72125 Biochemical identification
of isolates 122
722 Enterococcus 123
723 Aerococcus - 123
724 Gemella - :: 123
7 3 Peptococcaceae 124
References 124
8 Gram-positive rods isolated by simple cultivation 127
8 1 Regular nonacid-fast, nonsporogenic rods 128
811 Obligate aerobic regular nonsporogenic rods 128
812 Facultatively anaerobic regular nonsporogenic rods 130
8121 Lactobacillus 130
8122 Erysipelothrix 131
81221 Characteristics of infection 131
81222 Characteristics of the agent 131
8123 Listeria 132
81231 Characteristics of infection 132
81232 Characteristics of the agent 133
8 2 Coryneform bacteria 134
821 Corynebacterium spp 134
8211 Characteristics of infection 134
8212 Characteristics of the agent 137
82121 Morphology 137
82122 Cultivation 138
82123 Isolation sites 138
82124 Differentiation and identification 138
822 Arcanobacterium 138
823 Rhodococcus 139
824 Oerskovia 139
825 Eubacterium 139
8 3 Gram-positive rods forming mycelium or hyphae 140
831 Actinomyces spp 140
8311 Characteristics of infection 140
8312 Characteristics of the agent 140
8 4 Gram-positive sporogenic rods 141
841 Bacillus 141
8411 Characteristics of infection 141
8412 Characteristics of the agent 142
842 Clostridium 143
8421 Characteristics of infection 143
8422 Characteristics of the agent 145
,4 221 Morphology 145
84222 Cultivation : 146
84223 Isolation sites 146
84224 Differentiation and identification 146
84225 Toxin detection 147
References 150
Gram-positive rods not cultivated by simple techniques 153
9 1 Mycobacterium spp 154
911 Characteristics of infection 154
912 Characteristics of the agent 154
9121 Morphology 154
9122 Cultivation 154
9123 Isolation sites 156
9124 Differentiation and identification 156
913 Safety 156
9 2 Clostridium piliforme 158
921 Characteristics of infection 158
922 Characteristics of the agent 159
9221 Morphology 159
9222 Cultivation 159
9223 Staining 160
9224 Differentiation and identification 160
9225 Serology 160
9226 Molecular biology 160
References 161
10 Facultatively anaerobic Gram-negative bacteria 165
10 1 Enterobacteriaceae 166
10 1 1 Characteristics of infection 166
10 111 Escherichia 167
10 112 Citrobacter 167
10 113 Salmonella 168
10 114 Klebsiella 168
10 115 Enterobacter 168
10 116 Proteus 168
10 117 Morganella 168
10 118 Yersinia 169
10 119 Other species 169
10 1 2 Characteristics of the agent 169
10 121 Sampling and cultivation 169
10 122 Identification 171
10 1 3 Safety 176
10 2 Pasteurellaceae 177
10 2 1 Pasteurella 177
10 211 Characteristics of inaction 177
10 212 Characteristics of the agent 177
10 2121 Morphology 177
10 2122 Cultivation 178
10 2123 Isolation sites 178
10:2 124 Differentiation and identification 178
10 2125 Serology 178
10 2 2 Actinobacillus 179
10 221 Characteristics of infection 179
10 222 Characteristics of the agent 180
10 2221 Morphology 180
, 10 2222 Cultivation 180
10 2223 Isolation sites 180
10 2224 Differentiation and identification 180
10 2225 Serology : 181
10 2226 Molecular biology 181
10 2 3 Haemophilus 181
10 231 Characteristics of infection 181
10 232 Characteristics of the agent 181
10 3 Streptobacillus moniliformis 182
10 3 1 Characteristics of infection 182
10 3 2 Characteristics of the agent 184
10 321 Morphology 184
10 322 Cultivation 184
10 323 Isolation sites : 184
10 324 Differentiation and identification 184
10 325 Serology 184
10 4 Vibrionaceae 185
References 186
11 Obligate aerobic Gram-negative rods 191
11 1 Motile, aerobic, Gram-negative rods 192
11 1 1 Pseudomonas 192
11 111 Characteristics of infection 192
11 112 Characteristics of the agent 194
11 1121 Morphology 194
11 1122 Cultivation 195
11 1123 Isolation sites 195
11 1124 Differentiation and identification 195
11 1125 Serology 195
11 1 2 Xanthomonas, Sphingomonas, Chryseotnonas,
Agrobacterium, and Burkholderia 195
11 1 3 Bordetella 197
11 131 Characteristics of infection 197
11 132 Characteristic of the agent 197
11 1321 Morphology 197
11 1322 Cultivation 197
11 1323 Isolation sites 197
, 11 1324 Differentiation and identification 197
11 1325 Serology 197
11 2 Non-motile, aerobic, Gram-negative rods that can
easily be cultivated 198
11 2 1 Acinetobacter 198
11 2 2 Flavobacterium 198
11 2 3 Weeksella 198
11 3 Non-motile, aerobic, Gram-negative rods that
are difficult to cultivate 198
11 3 1 Francisella 200
11 311 Characteristics of infection 200
11 312 Characteristics of the agent 200
11 313 Safety 200
References 201
12 Obligate anaerobic and microaerophilic
Gram-negative bacteria 203
12 1 Bacteroidaceae 204
12 1 1 Fusobacterium 204
12 111 Characteristics of infection 204
12 112 Characteristics of the agent 204
12 112 1_Morphology 204
12 1122 Cultivation 206
12 1123 Differentiation and identification 206
12 2 Microaerophilic curved bacteria 206
12 2 1 Campylobacter 206
12 211 Characteristics of infection 206
12 212 Characteristics of the agent 208
12 2121 Morphology 208
12 2122 Cultivation 208
12 2123 Differentiation and identification 208
12 213 Safety 209
12 2 2 Helicobacter 209
12 221 Characteristics of infection 209
12 222 Characteristics of the agent 210
12 2221 Morphology 210
12 2222 Isolation sites and cultivation 210
12 2223 Differentiation and identification 210
12 2224 Serology 211
12 2225 Molecular biology 211
12 3 Cilia-associated respiratory bacillusQ 211
12 3 1 Characteristics of infection 211
12 3 2 Characteristics of the agent 212
12 321 Isolation 212
12 322 Identification 212
References : 212
13 Spirochetes 215
13 1 Treponema 216
13 1 1 Characteristics of infection 216
13 1 2 Characteristics of the agent 216
13 121 Morphology 216
13 122 Microscopy 216
13 123 Cultivation 217
13 124 Serology 217
13 125 Molecular biology 217
13 2 Leptospira 218
13 2 1 Characteristics of infection 218
13 2 2 Characteristics of the agent 218
13 221 Microscopy 218
13 222 Cultivation 219
13 223 Differentiation and identification 219
13 224 Serology 220
13 2 3 Safety 220
13 3 Spirillum minus 220
References 220
14 MoUicutes 223
14 1 Mycoplasma 223
14 1 1 Characteristics of infection 223
14 1 2 Characteristics of the agent 225
14 121 Morphology 225
14 122 Cultivation 225
14 123 Identification 228
14 124 Serology 228
14 2 Acholeplasma 229
References 229
Appendix I Producers of Reagents for Laboratory
Animal Bacteriology 233
Appendix II Biosafety Levels for Microbiological Laboratories 239
Index Q 245
|
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| spelling | Hansen, Axel Kornerup 1958- Verfasser (DE-588)1070478601 aut Handbook of laboratory animal bacteriology Axel Kornerup Hansen Boca Raton [u.a.] CRC Press 2000 255 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Bacteriology cabt Animaux de laboratoire - Maladies - Diagnostic Animaux de laboratoire - Santé Bactériologie vétérinaire - Technique Animal Diseases microbiology Bacterial Infections veterinary Laboratory Techniques and Procedures Laboratory animals Diseases Diagnosis Laboratory animals Health Veterinary bacteriology Technique Bakteriologie (DE-588)4004304-6 gnd rswk-swf Versuchstiere (DE-588)4078861-1 gnd rswk-swf Versuchstiere (DE-588)4078861-1 s Bakteriologie (DE-588)4004304-6 s DE-604 HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008828149&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hansen, Axel Kornerup 1958- Handbook of laboratory animal bacteriology Bacteriology cabt Animaux de laboratoire - Maladies - Diagnostic Animaux de laboratoire - Santé Bactériologie vétérinaire - Technique Animal Diseases microbiology Bacterial Infections veterinary Laboratory Techniques and Procedures Laboratory animals Diseases Diagnosis Laboratory animals Health Veterinary bacteriology Technique Bakteriologie (DE-588)4004304-6 gnd Versuchstiere (DE-588)4078861-1 gnd |
| subject_GND | (DE-588)4004304-6 (DE-588)4078861-1 |
| title | Handbook of laboratory animal bacteriology |
| title_auth | Handbook of laboratory animal bacteriology |
| title_exact_search | Handbook of laboratory animal bacteriology |
| title_full | Handbook of laboratory animal bacteriology Axel Kornerup Hansen |
| title_fullStr | Handbook of laboratory animal bacteriology Axel Kornerup Hansen |
| title_full_unstemmed | Handbook of laboratory animal bacteriology Axel Kornerup Hansen |
| title_short | Handbook of laboratory animal bacteriology |
| title_sort | handbook of laboratory animal bacteriology |
| topic | Bacteriology cabt Animaux de laboratoire - Maladies - Diagnostic Animaux de laboratoire - Santé Bactériologie vétérinaire - Technique Animal Diseases microbiology Bacterial Infections veterinary Laboratory Techniques and Procedures Laboratory animals Diseases Diagnosis Laboratory animals Health Veterinary bacteriology Technique Bakteriologie (DE-588)4004304-6 gnd Versuchstiere (DE-588)4078861-1 gnd |
| topic_facet | Bacteriology Animaux de laboratoire - Maladies - Diagnostic Animaux de laboratoire - Santé Bactériologie vétérinaire - Technique Animal Diseases microbiology Bacterial Infections veterinary Laboratory Techniques and Procedures Laboratory animals Diseases Diagnosis Laboratory animals Health Veterinary bacteriology Technique Bakteriologie Versuchstiere |
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