Identification and characterization of mutants constitutively expressing systemic acquired resistance in Arabidopsis thaliana:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | German |
| Veröffentlicht: |
1999
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 179 S. Ill., graph. Darst. |
Internformat
MARC
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| 001 | BV012887523 | ||
| 003 | DE-604 | ||
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| 007 | t | ||
| 008 | 991123s1999 gw ad|| m||| 00||| ger d | ||
| 016 | 7 | |a 957640668 |2 DE-101 | |
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| 035 | |a (DE-599)BVBBV012887523 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a ger | |
| 044 | |a gw |c DE | ||
| 049 | |a DE-355 |a DE-83 |a DE-188 | ||
| 100 | 1 | |a Maleck, Klaus |d 1972- |e Verfasser |0 (DE-588)121471705 |4 aut | |
| 245 | 1 | 0 | |a Identification and characterization of mutants constitutively expressing systemic acquired resistance in Arabidopsis thaliana |c vorgelegt von Klaus Maleck |
| 264 | 1 | |c 1999 | |
| 300 | |a XII, 179 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Köln, Univ., Diss., 1999 | ||
| 650 | 0 | 7 | |a Mutante |0 (DE-588)4256709-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Abwehrreaktion |0 (DE-588)4416206-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Ackerschmalwand |0 (DE-588)4141299-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Genkartierung |0 (DE-588)4123274-4 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Ackerschmalwand |0 (DE-588)4141299-0 |D s |
| 689 | 0 | 1 | |a Abwehrreaktion |0 (DE-588)4416206-6 |D s |
| 689 | 0 | 2 | |a Mutante |0 (DE-588)4256709-9 |D s |
| 689 | 0 | 3 | |a Genkartierung |0 (DE-588)4123274-4 |D s |
| 689 | 0 | |5 DE-604 | |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-008772176 | |
Datensatz im Suchindex
| _version_ | 1807683450660978688 |
|---|---|
| adam_text |
IN
TABLE
OF
CONTENTS
1.
INTRODUCTION
P.
1
1.1
THE
MODEL
SYSTEM
P.
1
1.1.1
MOLECULAR
TOOLS
IN
ARABIDOPSIS
P.
2
1.1.2
PATHOGENS
ON
ARABIDOPSIS
P.
4
1.1.2.1
PSEUDOMONAS
SYRINGAE
AND
THE
GENE-FOR-GENE
RELATIONSHIP
P.
5
1.1.2.2.
PERONOSPORA
PARASITICA
AND
R
GENE
EVOLUTION
P.
7
1.1.2.3
ERYSIPHE
CICHORACEARUM,
THE
FUNGAL
INFECTION
CYCLE
AND
P.
7
SECONDARY
METABOLITES
1.2
THE
SYSTEMIC
ACQUIRED
RESISTANCE
SIGNALING
CASCADE
P.
9
1.2.1
INDUCTION
OF
SAR
P.
9
1.2.2
SAR
MARKER
PROTEINS
P.
11
1.2.3
THE
ROLE
OF
SALICYLIC
ACID
IN
SAR
P.
12
1.2.4
OTHER
INDUCIBLE
DEFENSE
RESPONSES
AND
THEIR
RELATIONSHIP
TO
P.
13
SAR
1.2.5
GENETIC
DISSECTION
OF
SAR
P.
16
1.3
GOAL
OF
THIS
STUDY
P.
17
2.
MATERIALS
AND
METHODS
P.
18
2.1
CHEMICALS,
ENZYMES
AND
RADIOISOTOPES
P.
18
2.2
COMMON
MEDIA
AND
BUFFERS
P.
19
2.3.
BIOLOGICAL
MATERIALS
P.
21
2.3.1
PLANTS
P.
21
2.3.2
MICROORGANISMS
P.
22
2.3.2.1
E.
COLI
STRAINS
P.
22
2.3.2.2
YEAST
STRAINS
P.
22
2.3.2.3
AGROBACTERIUM
TUMEFACIENS
P.
22
IV
2.3.2.4
PSEUDOMONAS
SYRINGAE STRAINS
P.
22
2.3.2.5
PERONOSPORA
PARASITICA
P.
23
2.3.2.6
ERYSIPHE
CICHORACEARUM
P.
23
2.3.3
VECTORS
P.
23
2.3.3.1
PLASMIDS
P.
23
2.3.3.2
PHAGEMIDS
P.
24
2.3.3.3
LIBRARIES
AND
STOCK
COLLECTIONS
P.
24
2.4
GENERAL
LABORATORY
TECHNIQUES
P.
24
2.4.1
RESTRICTION
ENDONUCLEASE
DIGEST
OF
DNA
P.
25
2.4.2
PURIFICATION
OF
DNA
FRAGMENTS
P.
25
2.4.3
LIGATION
P.
25
2.4.4 PREPARATION
OF
HEAT-SHOCK
COMPETENT
E.
COLI
CELLS
P.
25
2.4.5
TRANSFORMATION
OF
COMPETENT
E.
COLI CELLS
P.
26
2.4.6
TRANSFORMATION
OF
COMPETENT
AGROBACTERIUM
CELLS
P.
26
2.4.7
PLASMID
MINIPREPARATION
P.
27
2.4.8
DNA
SEQUENCING
P.
27
2.5
PLANT
CARE
P.
28
2.5.1
PLANT
GROWTH
CONDITIONS
P.
28
2.5.2
POLLINATION
P.
28
2.5.3
TRANSFORMATION
OF
ARABIDOPSIS
THALIANA
BY
AGROBACTERIUM
P.
28
2.5.4 TREATMENT
OF
PLANTS
WITH
CHEMICAL
INDUCERS
OF
SAR
P.
29
2.5.5
LUCIFERASE
ACTIVITY
DETERMINATION
IN
VIVO
P.
29
2.6
PATHOGEN
TREATMENT
P.
29
2.6.1
TREATMENT
WITH
AND
CULTURE
OF
PERONOSPORA
P.
29
2.6.2 ERYSIPHE
INOCULATIONS
P.
30
2.6.3
PSEUDOMONAS
INFECTIONS
P.
30
2.7
STAINING
METHODS
P.
31
2.7.1
TRYPAN
BLUE
STAINING
FOR
CELL
DEATH
AND
FUNGAL
INFECTION
P.
31
2.7.2
3,3
'
-DIHEXYLOCARBOCYANINE
IODIDE
[DIOC
6
(3)]
STAINING
FOR
P.
31
ERYSIPHE
2.7.3
CALLOSE
STAINING
P.
31
2.8
BIOCHEMICAL METHODS
P.
32
2.8.1
LUCIFERASE
IN
VITRO ASSAY
P.
32
2.8.2
PAL
ENZYME
ACTIVITY
ASSAY
P.
32
2.8.3
SALICYLIC
ACID
EXTRACTION
P.
33
2.8.4
CAMALEXIN
EXTRACTION
P.
33
2.9
EXTRACTION
OF
NUCLEIC
ACIDS
P.
34
2.9.1
EXTRACTION
OF
PLANT
RNA
P.
34
2.9.2
POLYA
RNA
PURIFICATION
P.
34
2.9.3
PLANT
DNA
MINIPREPARATION
P.
35
2.9.4
PLANT
DNA
EXTRACTION
FOR
SOUTHERN
BLOT
ANALYSIS
AND
POOLED
P.
35
PROGENY
ANALYSIS
(F3
POPULATIONS)
2.9.5
BAC
DNA
MINIPREPARATION
P.
36
2.9.6
BAC
DNA
MAXIPREPARATION
P.
36
2.9.7
YAC
DNA
PREPARATION
P.
37
2.10
ANALYSIS
OF
MEMBRANE-BOUND
MACROMOLECULES
P.
37
2.10.1
ANALYSIS
OF
RNA
P.
37
2.10.2
SOUTHERN
BLOT
ANALYSIS
P.
39
2.10.3
DOT
BLOT
ANALYSIS
P.
40
2.10.4
HYBRIDIZATION
OF
BAC
FILTERS
AND
COLONY
FILTERS
P.
40
2.10.5
WESTERN
BLOT
ANALYSIS
P.
40
2.11
PCR
PROTOCOLS
AND
GENETIC
MARKERS
P.
41
2.11.1
SSLP
PCR
CONDITIONS
P.
41
2.11.2
CAPS
MARKER
PCR
CONDITIONS
AND
OTHER
PCRS
P.
41
2.11.3
LINKAGE
ANALYSIS
P.
42
2.11.4
LONG
RANGE
PCR
P.
42
2.11.5
OTHER
TECHNIQUES
FOR
POLYMORPHISM
DETECTION
P.
43
2.11.6
PREPARATION
OF
BAC
ENDS
BY
INVERSE
PCR
P.
43
2.11.7
THERMAL
ASYMMETRIC
INTERLACED
PCR
OF
T-DNA
JUNCTIONS
P.
44
2.12
GENOMICS
TECHNOLOGIES
P.
44
2.12.1
ARRAYING LIBRARIES
P.
44
2.12.2 TWO-DIMENSIONAL
PROTEIN
GEL
ELECTROPHORESIS
P.
45
VI
2.12.3
DNA
MICROARRAY
P.
46
3.
RESULTS
P.
48
3.1
PR
1
/LUCIFERASE LINE
CONSTRUCTION
AND
CHARACTERIZATION
P.
48
3.1.1.
PR-1
PHYLOGENY
P.
48
3.1.2
CLONING
AND
CHARACTERIZATION
OF
THE
TRANSGENIC
PR-7/LUC
LINE
P.
50
3.1.2.1
CHEMICAL
INDUCTION
OF
THE
PR-7/LUCIFERASE
GENE
P.
51
3.1.2.2
BIOLOGICAL
INDUCTION
OF
THE
P7?-7/LUCIFERASE
LINE
P.
53
3.2
MUTAGENESIS
AND
MUTANT
SCREEN
P.
53
3.3
CHARACTERIZATION
OF
CIM
MUTANTS
P.
57
3.3.1
HISTOLOGICAL
CHARACTERIZATION
OF
THE
CIM
MUTANTS
P.
57
3.3.2
GENETIC
CHARACTERIZATION
OF
THE
CIM
MUTANTS
P.
59
3.3.3
BIOCHEMICAL
CHARACTERIZATION
OF
THE
CIM
MUTANTS
P.
61
3.3.3.1
CIM
MUTANTS
ACCUMULATE
SALICYLIC
ACID
P.
61
3.3.3.2
OTHER
PHENYLPROPANOIDS
IN
CIM
MUTANTS
P.
62
3.3.3.3
CIM
MUTANTS
CAN
ACCUMULATE
LOW
LEVELS
OF
CAMALEXIN
P.
62
3.3.4
CIM MUTANTS
EXHIBIT
RESISTANCE
TO PATHOGENIC MICROORGANISMS
P.
63
3.3.4.1
MOST
CIM
MUTANTS
ARE
RESISTANT
TO
PERONOSPORA
PARASITICA
P.
63
3.3.4.2
A
SUBSET
OF
CIM
MUTANTS
IS
RESISTANT
TO
ERYSIPHE
CICHORACEARUM
P.
66
3.3.4.3
SOME
CIM
MUTANTS
EXHIBIT
A
SIGNIFICANT
RESISTANCE
TO
VIRULENT
P.
66
PSEUDOMONAS
SYRINGAE
PATHOVARS
3.3.5
MOLECULAR
CHARACTERIZATION
OF
CIM
MUTANTS
P.
68
3.3.5.1
NORTHERN BLOT
ANALYSIS
P.
68
3.3.5.2
DNA
MICROARRAY
P.
75
3.3.5.3
TWO-DIMENSIONAL
PROTEIN
GEL
ELECTROPHORESIS
OF
CIM
MUTANTS
P.
91
3.4
MAPPING
AND
CLONING
OF
CIM
MUTANTS
P.
93
3.4.1
PHENOTYPING
OF
MUTANT
DM!
13
P.
94
3.4.2
GENETIC
MAPPING
OF
CIM713
P.
97
3.4.3
PHYSICAL
MAPPING
OF
CIZN713
P.
99
3.4.4
GENETIC
MARKER
DEVELOPMENT
AND
FINE
MAPPING
OF
CIM713
P.
100
VII
3.4.5
CLONING
OF
FLANKING
DNA
IN
CIM
MUTANTS
GENERATED
BY
T-DNA
P.
107
TAGGING
3.5
EPISTASIS
STUDIES
WITH
CIM
MUTANTS
P.
110
3.5.1
ANALYSIS
OF
CIM
MUTANTS
IN
NAHG
BACKGROUNDS
P.
110
3.5.2
GENE
PROFILING
FOR
CIM
MUTANTS
IN
NAHG
BACKGROUNDS
P.
113
4
DISCUSSION
P.
118
4.1
PRINCIPLE
OF
THE
SCREEN
FOR
CIM
MUTANTS
P.
119
4.2 GENETICS
OF
CIM
MUTANTS
P.
120
4.3
NATURE
OF
CIM
MUTANTS
P.
122
4.4 EPISTASIS
STUDIES
AND
THE
RELATIONSHIP
OF
CIM
MUTANTS
TO
SALICYLIC ACID
P.
132
4.5
CONCLUSION
AND
FUTURE
STUDIES
P.
134
5
SUMMARY/ZUSAMMENFASSUNG
P.
138
6
REFERENCES
P.
142
7
APPENDIX
P.
173 |
| any_adam_object | 1 |
| author | Maleck, Klaus 1972- |
| author_GND | (DE-588)121471705 |
| author_facet | Maleck, Klaus 1972- |
| author_role | aut |
| author_sort | Maleck, Klaus 1972- |
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| bvnumber | BV012887523 |
| ctrlnum | (OCoLC)64648193 (DE-599)BVBBV012887523 |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV012887523 |
| illustrated | Illustrated |
| indexdate | 2024-08-18T00:34:38Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008772176 |
| oclc_num | 64648193 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-83 DE-188 |
| owner_facet | DE-355 DE-BY-UBR DE-83 DE-188 |
| physical | XII, 179 S. Ill., graph. Darst. |
| publishDate | 1999 |
| publishDateSearch | 1999 |
| publishDateSort | 1999 |
| record_format | marc |
| spelling | Maleck, Klaus 1972- Verfasser (DE-588)121471705 aut Identification and characterization of mutants constitutively expressing systemic acquired resistance in Arabidopsis thaliana vorgelegt von Klaus Maleck 1999 XII, 179 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 1999 Mutante (DE-588)4256709-9 gnd rswk-swf Abwehrreaktion (DE-588)4416206-6 gnd rswk-swf Ackerschmalwand (DE-588)4141299-0 gnd rswk-swf Genkartierung (DE-588)4123274-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ackerschmalwand (DE-588)4141299-0 s Abwehrreaktion (DE-588)4416206-6 s Mutante (DE-588)4256709-9 s Genkartierung (DE-588)4123274-4 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008772176&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Maleck, Klaus 1972- Identification and characterization of mutants constitutively expressing systemic acquired resistance in Arabidopsis thaliana Mutante (DE-588)4256709-9 gnd Abwehrreaktion (DE-588)4416206-6 gnd Ackerschmalwand (DE-588)4141299-0 gnd Genkartierung (DE-588)4123274-4 gnd |
| subject_GND | (DE-588)4256709-9 (DE-588)4416206-6 (DE-588)4141299-0 (DE-588)4123274-4 (DE-588)4113937-9 |
| title | Identification and characterization of mutants constitutively expressing systemic acquired resistance in Arabidopsis thaliana |
| title_auth | Identification and characterization of mutants constitutively expressing systemic acquired resistance in Arabidopsis thaliana |
| title_exact_search | Identification and characterization of mutants constitutively expressing systemic acquired resistance in Arabidopsis thaliana |
| title_full | Identification and characterization of mutants constitutively expressing systemic acquired resistance in Arabidopsis thaliana vorgelegt von Klaus Maleck |
| title_fullStr | Identification and characterization of mutants constitutively expressing systemic acquired resistance in Arabidopsis thaliana vorgelegt von Klaus Maleck |
| title_full_unstemmed | Identification and characterization of mutants constitutively expressing systemic acquired resistance in Arabidopsis thaliana vorgelegt von Klaus Maleck |
| title_short | Identification and characterization of mutants constitutively expressing systemic acquired resistance in Arabidopsis thaliana |
| title_sort | identification and characterization of mutants constitutively expressing systemic acquired resistance in arabidopsis thaliana |
| topic | Mutante (DE-588)4256709-9 gnd Abwehrreaktion (DE-588)4416206-6 gnd Ackerschmalwand (DE-588)4141299-0 gnd Genkartierung (DE-588)4123274-4 gnd |
| topic_facet | Mutante Abwehrreaktion Ackerschmalwand Genkartierung Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008772176&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT maleckklaus identificationandcharacterizationofmutantsconstitutivelyexpressingsystemicacquiredresistanceinarabidopsisthaliana |