Transcription factor protocols:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2000
|
| Series: | Methods in molecular biology
130 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | X, 306 S. Ill., graph. Darst. |
| ISBN: | 0896035735 |
Staff View
MARC
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| 245 | 1 | 0 | |a Transcription factor protocols |c ed. by Martin J. Tymms |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2000 | |
| 300 | |a X, 306 S. |b Ill., graph. Darst. | ||
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| 490 | 1 | |a Methods in molecular biology |v 130 | |
| 650 | 4 | |a Sequence Analysis, DNA |x methods | |
| 650 | 4 | |a Transcription Factors |x analysis | |
| 650 | 4 | |a Transcription factors |x Laboratory manuals | |
| 650 | 4 | |a Transfection |x methods | |
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Record in the Search Index
| _version_ | 1804127542532112384 |
|---|---|
| adam_text | Contents
Preface v
Contributors ix
1 Isolation of Target Gene Promoter/Enhancer Sequences by
Whole Genome PCR Method
Dennis K. Watson, Richard Kitching, Calvin Vary,
Ismail Kola, and Arun Seth 1
2 In Vivo Footprinting Using UV Light and Ligation Mediated PCR
Gerd P. Pfeifer and Stella Tommasi 13
3 Identification of DNasel Hypersensitive Sites Within Nuclei
Peter N. Cockerill 29
4 Analysis of In Vivo Methylation
Hans Henrik M. Dahl and Wendy M. Hutchison 47
5 Detection of Transcription Factor Partners with a Yeast One
Hybrid Screen
Michael Sieweke 59
6 Inverse PCR (IPCR) for Obtaining Promoter Sequence
Tony Triglia 79
7 PCR Directed Linker Scanning Mutagenesis
Kurt Gustin and Robert D. Burk 85
8 Transfection Technologies
Elaine T. Schenborn 91
9 The Use of Particle Mediated Gene Transfer for the Study
of Promoter Activity in Somatic Tissues
Charles M. Nicolet and Ning Sun Yang 103
10 Optimizing Electroporation Conditions for the Transformation
of Mammalian Cells
William C. Heiser 117
11 Calcium Phosphate Transfection of Mammalian Cultured Cells
Elaine T. Schenborn and Virginia Goiffon 135
vii
viii Contents
12 DEAE Dextran Transfection of Mammalian Cultured Cells
Elaine T. Schenborn and Virginia Goiffon 147
13 Liposome Mediated Transfection of Mammalian Cells
Elaine T. Schenborn and Jennifer Oler 155
14 Assays for Transcriptional Activity Based on the Luciferase
Reporter Gene
S. Roy Himes and M. Frances Shannon 165
15 Transient Transfection of Schneider Cells in the Study of
Transcription Factors
Guntram Suske 175
16 Triplex Forming Oligonucleotides and Their Use in the Analysis
of Gene Transcription
Marina Kochetkova and M. Frances Shannon 189
17 Expression and Purification of Histidine Tagged
Transcription Factors
Donna R. Cohen 203
18 Generation of Transcription Factors in Rabbit Reticulocyte Lysate
Depleted of Endogenous DNA Binding Protein
Ulrich Kruse, Thorsten T. Ebel, and Albrecht E. Sippel 227
19 Electrophoretic Mobility Shift Assays
Peter L. Molloy 235
20 In Vitro Promoter Analysis Using Nuclear Extracts and
G Free Cassette Vectors
Martin J. Tymms 247
21 In Vitro Transcription Using Competitor Oligonucleotides to Deplete
Specific Transcription Factors
FujikoWatt 257
22 Computer Software of Eukaryotic Promoter Analysis
DanS. Prestridge 265
Index 297
|
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| discipline | Biologie Chemie |
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| id | DE-604.BV012861138 |
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| indexdate | 2024-07-09T18:35:00Z |
| institution | BVB |
| isbn | 0896035735 |
| language | English |
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| physical | X, 306 S. Ill., graph. Darst. |
| publishDate | 2000 |
| publishDateSearch | 2000 |
| publishDateSort | 2000 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Transcription factor protocols ed. by Martin J. Tymms Totowa, NJ Humana Press 2000 X, 306 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 130 Sequence Analysis, DNA methods Transcription Factors analysis Transcription factors Laboratory manuals Transfection methods Transkriptionsfaktor (DE-588)4303350-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Transkriptionsfaktor (DE-588)4303350-7 s Methode (DE-588)4038971-6 s DE-604 Tymms, Martin J. Sonstige oth Methods in molecular biology 130 (DE-604)BV035362695 130 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008752049&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Transcription factor protocols Methods in molecular biology Sequence Analysis, DNA methods Transcription Factors analysis Transcription factors Laboratory manuals Transfection methods Transkriptionsfaktor (DE-588)4303350-7 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4303350-7 (DE-588)4038971-6 (DE-588)4143413-4 |
| title | Transcription factor protocols |
| title_auth | Transcription factor protocols |
| title_exact_search | Transcription factor protocols |
| title_full | Transcription factor protocols ed. by Martin J. Tymms |
| title_fullStr | Transcription factor protocols ed. by Martin J. Tymms |
| title_full_unstemmed | Transcription factor protocols ed. by Martin J. Tymms |
| title_short | Transcription factor protocols |
| title_sort | transcription factor protocols |
| topic | Sequence Analysis, DNA methods Transcription Factors analysis Transcription factors Laboratory manuals Transfection methods Transkriptionsfaktor (DE-588)4303350-7 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Sequence Analysis, DNA methods Transcription Factors analysis Transcription factors Laboratory manuals Transfection methods Transkriptionsfaktor Methode Aufsatzsammlung |
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