HIV protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1999
|
| Schriftenreihe: | Methods in molecular medicine
17 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIII, 421 S. graph. Darst. |
| ISBN: | 0896033694 |
Internformat
MARC
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| 245 | 1 | 0 | |a HIV protocols |c ed. by Nelson L. Michael ... |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 1999 | |
| 300 | |a XIII, 421 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular medicine |v 17 | |
| 650 | 2 | |a Biologie moléculaire - Méthode | |
| 650 | 7 | |a Biologie moléculaire |2 ram | |
| 650 | 7 | |a Diagnostic immunologique |2 ram | |
| 650 | 7 | |a HIV |2 gtt | |
| 650 | 7 | |a Infections à HIV - Prévention |2 ram | |
| 650 | 2 | |a Infections à VIH - prévention et contrôle | |
| 650 | 2 | |a Méthode immunologique | |
| 650 | 2 | |a Protocoles cliniques | |
| 650 | 7 | |a Sida - Manuels de laboratoire |2 ram | |
| 650 | 2 | |a Technique immunologique | |
| 650 | 2 | |a Virologie - Méthode | |
| 650 | 7 | |a Virus de l'immunodéficience humaine - Manuels de laboratoire |2 ram | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a AIDS (Disease) |x Laboratory manuals | |
| 650 | 4 | |a Clinical Protocols | |
| 650 | 4 | |a HIV (Viruses) |x Laboratory manuals | |
| 650 | 4 | |a HIV Infections |x prevention & control | |
| 650 | 4 | |a HIV infections |x Laboratory manuals | |
| 650 | 4 | |a Immunologic Techniques | |
| 650 | 4 | |a Molecular Biology |x methods | |
| 650 | 4 | |a Virology |x methods | |
| 650 | 0 | 7 | |a HIV |0 (DE-588)4200792-6 |2 gnd |9 rswk-swf |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-008587724 | ||
Datensatz im Suchindex
| _version_ | 1804127297183154176 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
Part I. Virology
1 Isolation and Expansion of HIV from Cells and Body Fluids
by Coculture
James R. Lane 3
2 Quantitative HIV Culture
James R. Lane /1
3 Quantitation of Cell Free HIV by Reverse Transcriptase Activity
James R. Lane 17
4 Determination of Syncytium lnducing Phenotype of Primary HIV 1
Isolates Using MT 2 Cells
Mark K. Louder and John R. Mascola 23
5 Determination of HIV 1 Chemokine Coreceptor Tropism Using
Transduced Human Osteosarcoma (HOS) Cells
Mark K. Louder and John R. Mascola 29
6 Use of Luciferase Reporter Viruses for Studying HIV Entry
Rong Liu, Benjamin Chen, and Nathaniel R. Landau 35
7 A Cell Cell Fusion Assay to Monitor HIV 1 Env Interactions
with Chemokine Receptors
Aimee L. Edinger and Robert W. Doms 41
8 Methods of Culturing HIV 1 from Semen
Bruce L. Gilliam 5/
Part II. Molecular Biology
9 Detection of HIV 1 Nucleic Acids by Southern Blotting
Richard A. McDonald 6/
10 Detection of HIV 1 Nucleic Acids by Northern Blotting
Richard A. McDonald and Christopher A. D. Smith 71
vii
viii Contents
11 Rapid Identification of Cloned HIV 1 Fragments
Wei Dong, Anindita Kar Roy, and Yen Li 83
12 PCR Amplification and Cloning of Virtually Full Length
HIV 1 Provirus
Mika Salminen 89
13 Quantitation of HIV 1 gag DNA and RNA From Single
Frozen Cell Pellets
Maryanne Vahey, Sandra C. Barrick, and Martin Nau 99
14 Quantitative PCR for HIV 1 Proviral DNA
Davide Zella and Paola Secchiero 119
15 Diagnosis and Direct Automated Sequencing of HIV 1
From Dried Blood Spots (DBS) Collected on Filter Paper
Sharon A. Cassol, Stanley Read, Bruce G. Weniger,
Richard Pilon, Barbara Leung, and Theresa Mo 125
16 Quantification of HIV 1 RNA in Dried Plasma Spots (DPS):
A Field Approach to Therapeutic Monitoring
Sharon A. Cassol, Francisco Diaz Mitoma, Richard Pilon,
Michelle Janes, and D. William Cameron 139
17 Quantitation of HIV 1 RNA in Genital Secretions
Bruce L. Gilliam 151
18 Detection of Nucleic Acids in Cells and Tissues by In Situ
Polymerase Chain Reaction
Omar Bagasra, Lisa E. Bobroski, Mohammad Amjad,
Matthew Memoli, and Maureen V. Abbey 165
19 Determination of the Promoter Activity of HIV 1 Using the
Chloramphenicol Acetyltransferase Reporter Gene Assay
Loretta Tse and Nelson L. Michael 185
20 In Vitro Techniques for Studies of HIV 1 Promoter Activity
Fabio Romerio and David M. Margolis 197
21 Detection of Polymorphisms in the HIV 1 Coreceptor CCR5
Using Single Strand Conformation Polymorphism
Maureen P. Martin and Mary Carrington 205
22 Sequence Specific Priming as a Rapid Screen for Known Mutations
Mary Carrington 213
23 Quantitation of HIV 1 Entry Cofactor Expression
James L. Riley and Richard G. Carroll 219
Contents ix
24 Retrovirus Mediated Gene Transduction of SupT1 Cells
Jen TsunJ. Lin and Jerome H. Kim 227
25 Cloning of HIV Single Strand DNA Binding Protein from
Human Lymphocyte Lambda gt11 Expression Library
with [32P] Oligomers
Sook Jin Hur and Derhsing Lai 231
26 Telomeric Terminal Restriction Fragment (TRF)
Sumesh Kaushal 237
Part III. Humoral Immunology
27 Serologic Analysis by Enzyme Linked
Immunosorbent Assay (ELISA)
Melissa Krider and Lawrence D. Loomis Price 245
28 Quantitative Immunoblotting with Fusion Proteins
Margaret M. Mitchell and Lawrence D. Loomis Price 255
29 Analysis of Antibody Interactions with HIV 1 Envelope
Expressed on the Surface of Acutely Infected H9 Cells
Thomas C. VanCott and Paul L. Hallberg 265
30 Analysis of Antibody Antigen Interactions Using
Surface Plasmon Resonance
Thomas C. VanCott 273
31 Measurement of HIV 1 Specific and Total Antibody
Secreting Cells by ELISPOT
Frederik W. van Ginkel, John L. VanCott, Robert Kaminski,
and Thomas C. VanCott 283
32 Linear Epitope Mapping by the PEPSCAN Method
Lawrence D. Loomis Price 293
33 Neutralization of HIV 1 Infection of Human Peripheral Blood
Mononuclear Cells (PBMC): Antibody Dilution Method
John R. Mascola 309
34 Neutralization of HIV 1 Infection of Human Peripheral Blood
Mononuclear Cells (PBMC): Infectivity Reduction Method
John R. Mascola 377
35 Collection and Processing of Mucosal Secretions from
HIV 1 Infected Women
Andrew W. Artenstein and Thomas C. VanCott 323
x Contents
36 Collection and Processing of Mucosal Secretions from Mice
Robert W. Kaminski and Thomas C. VanCott 329
Part IV. Cellular Immunology
37 Lymphocyte Proliferation Assay
Karl V. Sitz and Deborah L. Birx 343
38 HIV 1 Specific Cytotoxic T Cell Assays
Josephine H. Cox 355
39 HIV 1 Specific Antibody Dependent Cellular Cytotoxicity (ADCC)
Josephine H. Cox 373
40 Evaluation of Natural Killer Cell Activity
Josephine H. Cox 383
41 Generation and Expansion of HIV 1 CD4+
Antigen Specific T Cell Lines
Silvia Ratto Kim 391
42 Immunophenotyping and Assessment of Cell
Function by Three Color Flow Cytometry of
Peripheral Blood Lymphocytes
Stephen P. Perfetto and Gilbert McCrary 397
43 Detection of Apoptosis in HIV lnfected Cell Populations
using TUNEL
Patrick J. Blair and Stephen P. Perfetto 407
Index 413
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| isbn | 0896033694 |
| language | English |
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| physical | XIII, 421 S. graph. Darst. |
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| spelling | HIV protocols ed. by Nelson L. Michael ... Totowa, NJ Humana Press 1999 XIII, 421 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular medicine 17 Biologie moléculaire - Méthode Biologie moléculaire ram Diagnostic immunologique ram HIV gtt Infections à HIV - Prévention ram Infections à VIH - prévention et contrôle Méthode immunologique Protocoles cliniques Sida - Manuels de laboratoire ram Technique immunologique Virologie - Méthode Virus de l'immunodéficience humaine - Manuels de laboratoire ram Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten AIDS (Disease) Laboratory manuals Clinical Protocols HIV (Viruses) Laboratory manuals HIV Infections prevention & control HIV infections Laboratory manuals Immunologic Techniques Molecular Biology methods Virology methods HIV (DE-588)4200792-6 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf HIV (DE-588)4200792-6 s Methode (DE-588)4038971-6 s DE-604 Michael, Nelson L. Sonstige oth Methods in molecular medicine 17 (DE-604)BV011334960 17 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008587724&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | HIV protocols Methods in molecular medicine Biologie moléculaire - Méthode Biologie moléculaire ram Diagnostic immunologique ram HIV gtt Infections à HIV - Prévention ram Infections à VIH - prévention et contrôle Méthode immunologique Protocoles cliniques Sida - Manuels de laboratoire ram Technique immunologique Virologie - Méthode Virus de l'immunodéficience humaine - Manuels de laboratoire ram Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten AIDS (Disease) Laboratory manuals Clinical Protocols HIV (Viruses) Laboratory manuals HIV Infections prevention & control HIV infections Laboratory manuals Immunologic Techniques Molecular Biology methods Virology methods HIV (DE-588)4200792-6 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4200792-6 (DE-588)4038971-6 |
| title | HIV protocols |
| title_auth | HIV protocols |
| title_exact_search | HIV protocols |
| title_full | HIV protocols ed. by Nelson L. Michael ... |
| title_fullStr | HIV protocols ed. by Nelson L. Michael ... |
| title_full_unstemmed | HIV protocols ed. by Nelson L. Michael ... |
| title_short | HIV protocols |
| title_sort | hiv protocols |
| topic | Biologie moléculaire - Méthode Biologie moléculaire ram Diagnostic immunologique ram HIV gtt Infections à HIV - Prévention ram Infections à VIH - prévention et contrôle Méthode immunologique Protocoles cliniques Sida - Manuels de laboratoire ram Technique immunologique Virologie - Méthode Virus de l'immunodéficience humaine - Manuels de laboratoire ram Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten AIDS (Disease) Laboratory manuals Clinical Protocols HIV (Viruses) Laboratory manuals HIV Infections prevention & control HIV infections Laboratory manuals Immunologic Techniques Molecular Biology methods Virology methods HIV (DE-588)4200792-6 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Biologie moléculaire - Méthode Biologie moléculaire Diagnostic immunologique HIV Infections à HIV - Prévention Infections à VIH - prévention et contrôle Méthode immunologique Protocoles cliniques Sida - Manuels de laboratoire Technique immunologique Virologie - Méthode Virus de l'immunodéficience humaine - Manuels de laboratoire Wetenschappelijke technieken Wissenschaftliches Arbeiten AIDS (Disease) Laboratory manuals Clinical Protocols HIV (Viruses) Laboratory manuals HIV Infections prevention & control HIV infections Laboratory manuals Immunologic Techniques Molecular Biology methods Virology methods Methode |
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