Introduction to biological scanning electron microscopy:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Baltimore [u.a.]
Univ. Park Press
1978
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XVIII, 323 S. Ill., graph. Darst. |
| ISBN: | 0839111738 |
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MARC
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| 245 | 1 | 0 | |a Introduction to biological scanning electron microscopy |c M. A. Hayat |
| 264 | 1 | |a Baltimore [u.a.] |b Univ. Park Press |c 1978 | |
| 300 | |a XVIII, 323 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 4 | |a Microscopes électroniques à balayage | |
| 650 | 4 | |a Microscopy, Electron, Scanning |x instrumentation | |
| 650 | 4 | |a Microscopy, Electron, Scanning |x methods | |
| 650 | 4 | |a Scanning electron microscopes | |
| 650 | 4 | |a Specimen Handling |x methods | |
| 856 | 4 | 2 | |m SWB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008566567&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
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Record in the Search Index
| _version_ | 1804127265697562624 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
LIST OF FIGURES XI
LIST OF TABLES XV
PREFACE XVII
PART 1 INSTRUMENTATION
INTRODUCTION 3
HISTORY OF SCANNING ELECTRON MICROSCOPE DEVELOPMENT.. 9
PRINCIPLE OF SCANNING ELECTRON MICROSCOPY 13
ELECTRON BEAM AND SPECIMEN INTERACTION 15
SECONDARY ELECTRONS 15
BACKSCATTERED ELECTRONS 18
AUGER ELECTRONS 20
CHARACTERISTIC X-RAYS 21
CATHODOLUMINESCENCE 22
IMAGE FORMATION 25
SECONDARY ELECTRON IMAGING 25
BACKSCATTERED ELECTRON IMAGING 25 BACKSCATTERED ELECTRON DETECTORS 27
AUGER ELECTRON IMAGING 29
CHARACTERISTIC X-RAY IMAGING 31
CATHODOLUMINESCENT IMAGING 33
SCANNING ELECTRON MICROSCOPE 39
ELECTRON GUN 39
SIGNAL-TO-NOISE RATIO 40
BEAM CURRENT 40
LENSES 41
WORKING DISTANCE 41
FINAL APERTURE SIZE 42
ELECTRON PROBE 42
SPECIMEN STAGE 43
ANGLE OF SPECIMEN TILT 43
DETECTOR 44
OPERATION OF SEM 45
SCANNING ELECTRON MICROSCOPY 47
CONTRAST 47
RESOLUTION 51
MAGNIFICATION 53
EDGE EFFECTS 53
SPECIMEN CONTAMINATION IN SEM 54
REMOVAL OF CONTAMINATION 55
PREVENTION OF CONTAMINATION 56
IMAGE 2
VI CONTENTS
BEAM DAMAGE 57
VACUUM DAMAGE IN SEM 61
ELECTRICAL CHARGING OF SPECIMENS 62 CHARGING ARTIFACTS 66
LOW-VOLTAGE SCANNING ELECTRON MICROSCOPY 71
STEREOMICROSCOPY 73
SCANNING TRANSMISSION ELECTRON MICROSCOPE 75
POLYCHROMATIC IMAGES 77
PART 2 SPECIMEN PREPARATION
INTRODUCTION 81
SPECIMEN SIZE 85
SPECIMEN CLEANING 87
FIXATION 91
FIXATIVES 97
PREPARATION OF FIXATIVES 100
METHODS OF FIXATION 100
VASCULAR PERFUSION 101
APPARATUS FOR VASCULAR PERFUSION 104
METHODS OF VASCULAR PERFUSION 111
AORTA 111
CENTRAL NERVOUS SYSTEM 112
EMBRYO 114
HEART 114
KIDNEY 116
LIVER 116
LIVER (EMBRYOS AND VERY SMALL ANIMALS) 117 LUNG 118
LUNG (SMALL ANIMALS) 119
LUNG (HUMAN) BIOPSY SPECIMENS 121
MUSCLE (SKELETAL MUSCLE OF RAT HIND LIMB) 121 SPLEEN 123
TESTES 124
IMMERSION FIXATION 124
DRIPPING METHOD 127
INJECTION METHOD 128
ANESTHESIA 128
DRYING 131
AIR DRYING 131
CRITICAL POINT DRYING 131
PRINCIPLE OF CRITICAL POINT DRYING 132
SPECIMEN SHRINKAGE AND OTHER ARTIFACTS 134 DEHYDRATION FOR CRITICAL
POINT DRYING 136
IMAGE 3
CONTENTS VII
INTERMEDIATE FLUIDS 136
AMYL ACETATE 138
FREON TF 138
TRANSITION FLUIDS 139
SUBSTITUTION WITH TRANSITION FLUIDS 140
FREON TF TO FREON 13 140
AMYL ACETATE TO CO 2 141
FREON TF TO CO 2 142
ETHANOL TO CO 2 143
ACETONE TO CO 2 143
OPERATION OF A CRITICAL POINT DRYING APPARATUS 144 PROCESS OF CRITICAL
POINT DRYING 146
GENERAL PROCEDURE FOR CRITICAL POINT DRYING 146 STEP-BY-STEP PROCEDURE
FOR CRITICAL POINT DRYING FROM CO 2 147 CRITICAL POINT DRYING USING DRY
ICE 149
CONTAINERS FOR CRITICAL POINT DRYING 150
HEALTH AND SAFETY HAZARDS 162
FREEZE-DRYING 162
METHOD 163
ALTERNATE METHOD I 165
ALTERNATE METHOD II 166
FROZEN SPECIMENS 167
EXAMINATION OF INTERNAL SURFACES 171
CRYOFRACTURING 173
FROZEN RESIN CRACKING METHOD 175
BRITTLE FRACTURE BY LIQUID SHEAR 176
MICRODISSECTION IN SEM 177
SECTIONED SPECIMENS 178
PLANTS 178
ANIMALS 179
ION BEAM ETCHING 179
METHOD 182
RADIO-FREQUENCY SPUTTERING 183
METHOD 184
GLOW-DISCHARGE SPUTTERING 184
ISOLATED CELL ORGANELLES 185
CELL SURFACE LABELING METHODS 187
ANTIBODY ADSORBED TO LATEX SPHERES 188 ANTIBODY COVALENTLY LINKED TO
LATEX SPHERES 190 DEFINED SILICA SPHERES 192
HAPTEN-SANDWICH LABELING 192
GLUTARALDEHYDE COUPLING TECHNIQUES 194 TOBACCO MOSAIC VIRUS 195
BACTERIOPHAGE T 4 196
FERRITIN 197
HORSERADISH PEROXIDASE-LABELED ANTIBODY .. .198 CONCANAVALIN
A-HEMOCYANIN 199
IMAGE 4
CONTENTS
CONCANAVALIN A-HORSERADISH PEROXIDASE 202 FLUORESCEIN-LABELED ANTIBODY
203
COLLOIDAL GOLD 203
PREPARATION OF COLLOIDAL GOLD 204
PREPARATION OF CON A-GOLD 204
MARKING CELLS WITH CON A-GOLD 204
PREPARATION OF WGA GOLD 205
MARKING CELLS WITH WGA GOLD 205
SPECIMEN MOUNTING 207
SUBSTRATES FOR PARTICULATE SPECIMENS 213
SPECIMEN COATING 219
ELECTRICAL CONDUCTORS 219
COATING BY VACUUM EVAPORATION 222
EVAPORATION UNIT 222
COATING PROCEDURE 223
ALTERNATE COATING PROCEDURE 225
COATING OF FROZEN SPECIMENS 226
COATING BY SPUTTERING 227
IMPORTANT PARAMETERS 227
TEMPERATURE 228
THICKNESS 229
UNIFORMITY 229
PARTICLE SIZE 230
OPTIMIZATION OF CONDITIONS 230
PROCEDURE FOR TRIODE SPUTTER COATING 231
COATING BY POLYMER FILMS 232
METHOD 233
VAPOR INTENSITY AND COATING THICKNESS 233 ANTISTATIC AGENTS 237
UNCOATED SPECIMENS 239
OSMIUM TETROXIDE-THIOCARBOHYDRAZIDE- OSMIUM TETROXIDE METHOD 244
MODIFIED OSMIUM TETROXIDE-THIOCAR- BOHYDRAZIDE-OSMIUM TETROXIDE METHOD
244 POTASSIUM IODIDE-LEAD ACETATE METHOD 245 PREPARATION OF SOLUTIONS
245
TANNIC ACID-OSMIUM TETROXIDE METHOD 245 MODIFIED
TANNICACID-OSMIUMTETROXIDE METHOD 246 WET CHEMICAL METHOD 246
PREPARATION OF SOLUTION 246
OSMIUM TETROXIDE VAPOR METHOD 246
HEAVY METAL STAINING 249
METHODS 251
CORRELATIVE STUDIES 255
IMAGE 5
CONTENTS IX
ARTIFACTS ARISING DURING SPECIMEN PREPARATION 259
APPENDIX 265
REFERENCES 269
AUTHOR INDEX 305
SUBJECT INDEX 315
|
| any_adam_object | 1 |
| author | Hayat, M. A. 1936- |
| author_GND | (DE-588)129731447 |
| author_facet | Hayat, M. A. 1936- |
| author_role | aut |
| author_sort | Hayat, M. A. 1936- |
| author_variant | m a h ma mah |
| building | Verbundindex |
| bvnumber | BV012612975 |
| callnumber-first | Q - Science |
| callnumber-label | QH212 |
| callnumber-raw | QH212.S3 |
| callnumber-search | QH212.S3 |
| callnumber-sort | QH 3212 S3 |
| callnumber-subject | QH - Natural History and Biology |
| ctrlnum | (OCoLC)3447465 (DE-599)BVBBV012612975 |
| dewey-full | 578/.4/5 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 578 - Natural history of organisms |
| dewey-raw | 578/.4/5 |
| dewey-search | 578/.4/5 |
| dewey-sort | 3578 14 15 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Book |
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| id | DE-604.BV012612975 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:30:36Z |
| institution | BVB |
| isbn | 0839111738 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008566567 |
| oclc_num | 3447465 |
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| owner_facet | DE-83 DE-188 |
| physical | XVIII, 323 S. Ill., graph. Darst. |
| psigel | TUB-nveb |
| publishDate | 1978 |
| publishDateSearch | 1978 |
| publishDateSort | 1978 |
| publisher | Univ. Park Press |
| record_format | marc |
| spelling | Hayat, M. A. 1936- Verfasser (DE-588)129731447 aut Introduction to biological scanning electron microscopy M. A. Hayat Baltimore [u.a.] Univ. Park Press 1978 XVIII, 323 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Microscopes électroniques à balayage Microscopy, Electron, Scanning instrumentation Microscopy, Electron, Scanning methods Scanning electron microscopes Specimen Handling methods SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008566567&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hayat, M. A. 1936- Introduction to biological scanning electron microscopy Microscopes électroniques à balayage Microscopy, Electron, Scanning instrumentation Microscopy, Electron, Scanning methods Scanning electron microscopes Specimen Handling methods |
| title | Introduction to biological scanning electron microscopy |
| title_auth | Introduction to biological scanning electron microscopy |
| title_exact_search | Introduction to biological scanning electron microscopy |
| title_full | Introduction to biological scanning electron microscopy M. A. Hayat |
| title_fullStr | Introduction to biological scanning electron microscopy M. A. Hayat |
| title_full_unstemmed | Introduction to biological scanning electron microscopy M. A. Hayat |
| title_short | Introduction to biological scanning electron microscopy |
| title_sort | introduction to biological scanning electron microscopy |
| topic | Microscopes électroniques à balayage Microscopy, Electron, Scanning instrumentation Microscopy, Electron, Scanning methods Scanning electron microscopes Specimen Handling methods |
| topic_facet | Microscopes électroniques à balayage Microscopy, Electron, Scanning instrumentation Microscopy, Electron, Scanning methods Scanning electron microscopes Specimen Handling methods |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008566567&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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