Immunocytochemical methods and protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1999
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
115 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 465 S. Ill., graph. Darst. |
| ISBN: | 0896035700 0896038130 |
Internformat
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| 245 | 1 | 0 | |a Immunocytochemical methods and protocols |c ed. by Lorette C. Javois |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 1999 | |
| 300 | |a XII, 465 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 115 | |
| 650 | 4 | |a Hybridation in situ - Manuels de laboratoire | |
| 650 | 7 | |a Hybridation in situ |2 ram | |
| 650 | 7 | |a Immunocytochemie |2 gtt | |
| 650 | 4 | |a Immunocytochemistry - Technique | |
| 650 | 4 | |a Immunocytochimie - Manuels de laboratoire | |
| 650 | 7 | |a Immunocytochimie - Manuels de laboratoire |2 ram | |
| 650 | 2 | |a Immunohistochimie | |
| 650 | 4 | |a Inmunología | |
| 650 | 4 | |a Inmunoquímica | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a Immunocytochemistry |x Laboratory manuals | |
| 650 | 4 | |a Immunohistochemistry |x methods | |
| 650 | 4 | |a Immunohistochemistry |x methods |v laboratory manuals | |
| 650 | 4 | |a Immunohistochemistry |x standards | |
| 650 | 4 | |a In situ hybridization |x Laboratory manuals | |
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Datensatz im Suchindex
| _version_ | 1804127110448545792 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
Part I. Antibody Preparation
1 Overview of Antibody Use in Immunocytochemistry
Su Yau Mao, Lorette C. Javois, and Ute M. Kent 3
2 Purification of Antibodies Using Ammonium Sulfate Fractionation
or Gel Filtration
Ute M. Kent 11
3 Purification of Antibodies Using Ion Exchange Chromatography
Ute M. Kent 19
4 Purification of Antibodies Using Affinity Chromatography
Ute M. Kent 23
5 Purification of Antibodies Using Protein A Sepharose and FPLC
Ute M. Kent 29
6 Conjugation of Fluorochromes to Antibodies
Su Yau Mao 35
7 Biotinylation of Antibodies
Su Yau Mao 39
Part II. Tissue Preparation for Light Microscopic Analyses
8 Overview of Cell Fixatives and Cell Membrane Permeants
Melissa A. Melan 45
9 Preparation of Frozen Sections for Analysis
Gary L. Bratthauer 57
10 Processing of Cytological Specimens
Gary L. Bratthauer 63
11 Processing of Tissue Culture Cells
Gary L Bratthauer 71
12 Processing of Tissue Specimens
Gary L. Bratthauer 77
vii
viii Contents
13 Antigen Retrieval for Immunohistochemical Reactions in Routinely
Processed Paraffin Sections
Laszlo Krenacs, Tibor Krenacs, and Mark Raff eld 85
Part III: Light Microscopic Detection Systems
14 Overview of Fluorochromes
J. Michael Mullins 97
15 Direct Immunofluorescent Labeling of Cells
LoretteC. Javois 707
16 Fluorescence Labeling of Surface Antigens of Attached
or Suspended Tissue Culture Cells
Mark C. Willingham 113
17 Fluorescence Labeling of Intracellular Antigens of Attached
or Suspended Tissue Culture Cells
MarkC. Willingham 121
18 Fluorescent Labeling of Surface or Intracellular Antigens
in Whole Mounts
LoretteC. Javois 131
19 TUNEL Assay for Apoptotic Cells
Virginia M. Heatwole 141
20 Overview of Fluorescence Analysis with the Confocal Microscope
Liana Harvath 149
21 Overview of Laser Microbeam Applications as Related
to Antibody Targeting
P. Scott Pine 159
22 Overview of Fluorescence Photomicography
Lorette C. Javois and J. Michael Mullins 175
23 Overview of Antigen Detection Through Enzymatic Activity
Gary L. Bratthauer 181
24 The Peroxidase Antiperoxidase (PAP) Method and Other
All lmmunologic Detection Methods
Gary L. Bratthauer 191
25 The Avidin Biotin Complex (ABC) Method and Other Avidin Biotin
Binding Methods
Gary L. Bratthauer 203
26 Avidin Biotin Labeling of Cellular Antigens
in Cryostat Sectioned Tissue
Mark Raffeld and Elaine S. Jaffe 215
27 Multiple Antigen Immunostaining Procedures
Tibor Krenacs, Laszlo Krenacs, and Mark Raffeld 223
Contents ix
28 ELISA on Attachment Dependent or Suspension Grown Cells
Julie Ann Brent 235
29 Use of Immunogold with Silver Enhancement
Constance Oliver 241
Part IV: Fluorescence Activated Cell Sorter (FACS) Analyses
30 Overview of Flow Cytometry and Fluorescent Probes for Cytometry
Robert E. Cunningham 249
31 Tissue Disaggregation
Robert E. Cunningham 257
32 Indirect Immunofluorescent Labeling of Viable Cells
Robert E. Cunningham 261
33 Indirect Immunofluorescent Labeling of Fixed Cells
Robert E. Cunningham 265
34 Fluorescent Labeling of DNA
Robert E. Cunningham 271
35 Deparaffinization and Processing of Pathologic Material
Robert E. Cunningham 275
36 Assay for Phagocytosis
Liana Harvath and Douglas A. Terle 281
37 Assay for Filamentous Actin
Liana Harvath 291
38 Assay for Chemoattractant Binding
Liana Harvath, Robert R. Aksamit, and Robert E. Cunningham 299
39 Assay for Oxidative Metabolism
Liana Harvath and Douglas A. Terle 309
Part V: Colloidal Gold Detection Systems for Electron Microscopic Analyses
40 Fixation and Embedding
Constance Oliver and Maria CeliaJamur 319
41 Preparation of Colloidal Gold
Constance Oliver 327
42 Conjugation of Colloidal Gold to Proteins
Constance Oliver 331
43 Colloidal Gold/Streptavidin Methods
Constance Oliver 335
44 Pre Embedding Labeling Methods
Constance Oliver 341
45 Postembedding Labeling Methods
Constance Oliver 34 7
x Contents
Part VI: Applications for Nucleic Acid Localization by In Situ Hybridization
46 Overview of In Situ Hybridization
Roland M. Nardone 357
47 In Situ Hybridization to Human Chromosomes of an Alkaline
Phosphatase Labeled Centromeric Probe
Roland M. Nardone 365
48 Fluorescence In Situ Hybridization Using Whole Chromosome
Library Probes
Roland M. Nardone 371
49 In Situ RT PCR and Hybridization Techniques
Vishakha Thaker 379
Part VII: Special Applications for the Clinical Laboratory
50 Overview of the Clinical Immunohistochemistry Laboratory:
Regulations and Troubleshooting Guidelines
Patricia A. Fetsch and Andrea Abati 405
51 Overview of Immunohistochemical Approaches to the Differential
Diagnosis of Tumors
Dennis M. Frisman 415
52 Overview of Automated Immunostainers
Gilbert E. Herman, Edna A. Elfont, and Alton D. Floyd 439
Index 459
|
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| discipline | Biologie Medizin |
| edition | 2. ed. |
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| indexdate | 2024-07-09T18:28:08Z |
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| isbn | 0896035700 0896038130 |
| language | English |
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| physical | XII, 465 S. Ill., graph. Darst. |
| publishDate | 1999 |
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| series | Methods in molecular biology |
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| spelling | Immunocytochemical methods and protocols ed. by Lorette C. Javois 2. ed. Totowa, NJ Humana Press 1999 XII, 465 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 115 Hybridation in situ - Manuels de laboratoire Hybridation in situ ram Immunocytochemie gtt Immunocytochemistry - Technique Immunocytochimie - Manuels de laboratoire Immunocytochimie - Manuels de laboratoire ram Immunohistochimie Inmunología Inmunoquímica Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Immunocytochemistry Laboratory manuals Immunohistochemistry methods Immunohistochemistry methods laboratory manuals Immunohistochemistry standards In situ hybridization Laboratory manuals Immuncytochemie (DE-588)4161368-5 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Peptidsynthese (DE-588)4173715-5 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Immuncytochemie (DE-588)4161368-5 s Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s DE-604 Peptidsynthese (DE-588)4173715-5 s 1\p DE-604 Javois, Lorette C. Sonstige oth Methods in molecular biology 115 (DE-604)BV035362695 115 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008462726&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Immunocytochemical methods and protocols Methods in molecular biology Hybridation in situ - Manuels de laboratoire Hybridation in situ ram Immunocytochemie gtt Immunocytochemistry - Technique Immunocytochimie - Manuels de laboratoire Immunocytochimie - Manuels de laboratoire ram Immunohistochimie Inmunología Inmunoquímica Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Immunocytochemistry Laboratory manuals Immunohistochemistry methods Immunohistochemistry methods laboratory manuals Immunohistochemistry standards In situ hybridization Laboratory manuals Immuncytochemie (DE-588)4161368-5 gnd Methode (DE-588)4038971-6 gnd Molekularbiologie (DE-588)4039983-7 gnd Peptidsynthese (DE-588)4173715-5 gnd |
| subject_GND | (DE-588)4161368-5 (DE-588)4038971-6 (DE-588)4039983-7 (DE-588)4173715-5 (DE-588)4143413-4 |
| title | Immunocytochemical methods and protocols |
| title_auth | Immunocytochemical methods and protocols |
| title_exact_search | Immunocytochemical methods and protocols |
| title_full | Immunocytochemical methods and protocols ed. by Lorette C. Javois |
| title_fullStr | Immunocytochemical methods and protocols ed. by Lorette C. Javois |
| title_full_unstemmed | Immunocytochemical methods and protocols ed. by Lorette C. Javois |
| title_short | Immunocytochemical methods and protocols |
| title_sort | immunocytochemical methods and protocols |
| topic | Hybridation in situ - Manuels de laboratoire Hybridation in situ ram Immunocytochemie gtt Immunocytochemistry - Technique Immunocytochimie - Manuels de laboratoire Immunocytochimie - Manuels de laboratoire ram Immunohistochimie Inmunología Inmunoquímica Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Immunocytochemistry Laboratory manuals Immunohistochemistry methods Immunohistochemistry methods laboratory manuals Immunohistochemistry standards In situ hybridization Laboratory manuals Immuncytochemie (DE-588)4161368-5 gnd Methode (DE-588)4038971-6 gnd Molekularbiologie (DE-588)4039983-7 gnd Peptidsynthese (DE-588)4173715-5 gnd |
| topic_facet | Hybridation in situ - Manuels de laboratoire Hybridation in situ Immunocytochemie Immunocytochemistry - Technique Immunocytochimie - Manuels de laboratoire Immunohistochimie Inmunología Inmunoquímica Wetenschappelijke technieken Wissenschaftliches Arbeiten Immunocytochemistry Laboratory manuals Immunohistochemistry methods Immunohistochemistry methods laboratory manuals Immunohistochemistry standards In situ hybridization Laboratory manuals Immuncytochemie Methode Molekularbiologie Peptidsynthese Aufsatzsammlung |
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