Rapid detection of infectious agents:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
New York [u.a.]
Plenum Press
1998
|
| Series: | Infectious agents and pathogenesis
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XXIII, 216 S. Ill., graph. Darst. |
| ISBN: | 0306458489 |
Staff View
MARC
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| 245 | 1 | 0 | |a Rapid detection of infectious agents |c ed. by Steven Specter ... |
| 264 | 1 | |a New York [u.a.] |b Plenum Press |c 1998 | |
| 300 | |a XXIII, 216 S. |b Ill., graph. Darst. | ||
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| 490 | 0 | |a Infectious agents and pathogenesis | |
| 650 | 4 | |a Diagnostics moléculaires | |
| 650 | 4 | |a Microbiologie clinique | |
| 650 | 4 | |a Pathogenic microorganisms - Detection | |
| 650 | 4 | |a Diagnostic microbiology | |
| 650 | 4 | |a Genetic Techniques | |
| 650 | 4 | |a Immunologic Techniques | |
| 650 | 4 | |a Infection |x diagnosis | |
| 650 | 4 | |a Molecular diagnosis | |
| 650 | 4 | |a Polymerase Chain Reaction | |
| 650 | 4 | |a Virus Diseases |x diagnosis | |
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Record in the Search Index
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| adam_text | Contents
1. Genetically Engineered Cells in Diagnostic Virology
PAUL D. OLIVO
1. Introduction 1
1.1. History of Viral Culture 2
1.2. Viral Replication, Host Range, Susceptibility,
and Permissivity 2
2. Stable Transformation of Cells to Confer Viral
Susceptibility 3
3. Transgenic Cells that Facilitate Viral Detection 5
3.1. General Principles 5
3.2. Application to Specific Viral Families 9
4. Future Prospects 15
4.1. Engineering Cells Resistant to Viruses 15
4.2. Primary Cell Lines Derived from Transgenic Animals 15
5. Conclusion 15
References 16
2. Use of Synthetic Peptides in Microbial Diganostics
SUSANNE MODROW and HANS WOLF
1. Introduction 21
2. Methodology 22
2.1. Peptide Synthesis 22
xvii
xviii CONTENTS
2.2. Peptide Coupling 24
2.3. Identification of Antigenic Epitopes 25
3. Applications 27
3.1. Research 27
3.2. Routine Diganostics 28
3.3. Future Prospects 32
4. Conclusions 32
References 32
3. Flow Cytometric Analysis of Virally Infected Cells:
In Vitro and in Vivo Studies
JAMES J. McSHARRY
1. Introduction 39
2. A Brief Description of Flow Cytometry 40
3. Detection of Virally Infected Cells in Clinical Specimens 44
3.1. Human Immunodeficiency Virus (HIV) 44
3.2. Human Cytomegalovirus (HCMV) 44
3.3. Herpes Simplex Viruses 45
4. In Vitro Studies with Human Cytomegalovirus 46
4.1. Materials and Methods 46
4.2. Assay for the IC50 and IC90 of Antiviral Drugs
for Human Cytomegalovirus 48
4.3. Assay of Drug Sensitivity of Human Cytomegalovirus
to Ganciclovir 51
5. Future Uses of Flow Cytometry in Clinical Virology 54
References 55
4. Diagnostic Significance of Antibodies in Oral Secretions
SPENCER R. HEDGES, MICHAEL W. RUSSELL,
andJIRI MESTECKY
1. Introduction 57
2. IgA and the Mucosal Immune System 58
3. Origins of Salivary Immunoglobulins 60
CONTENTS xix
4. Diagnosis of Infection and Disease with
Salivary Antibodies 60
5. Practicalities and Limitations 63
6. Conclusions 64
References 64
5. Genetic Amplification Techniques for Diagnosing
Infectious Diseases
XIAOTIAN ZHENG and DAVID PERSING
1. Introduction 69
2. Target Amplification 70
2.1. Polymerase Chain Reaction 70
2.2. Transcription Based Amplification Systems 72
3. Probe Amplification Methods 74
4. Signal Amplification Techniques 76
5. Quantitative Nucleic Acid Amplification 76
6. Concluding Remarks 79
References 81
6. Ligase Chain Reaction for Detecting
Sexually Transmitted Diseases
CHARLOTTE A. GAYDOS and THOMAS C. QUINN
1. Introduction 83
2. Principle 84
3. Sample Preparation 85
4. Probe Set and Target Selection 86
5. Control of Contamination 86
6. LCR Applications 87
6.1. Chlamydia trachomatis 87
6.2. Neisseria gonorrhoeae 89
7. Specimen Transport and Alternative Specimen
Advantages 91
8. Summary 92
References 92
xx CONTENTS
7. Applications of the Polymerase Chain Reaction
DANNY L. WIEDBRAUK and RICHARD L. HODINKA
1. Introduction 97
2. The Polymerase Chain Reaction 97
3. Clinical Application of the Polymerase Chain Reaction. 99
3.1. Early Detection of Infection 99
3.2. Detecting Unculturable Infectious Agents 100
3.3. Detecting Slow Growing or Fastidious Agents 100
3.4. Detecting Infectious Agents Dangerous to Grow 101
3.5. Detecting Nonviable Agents 103
3.6. Resolving Indeterminate Serologies 104
3.7. Detecting Organisms Present in Low Numbers 104
3.8. Small Specimen Volumes 104
3.9. Predicting Antimicrobial/Antiviral Resistance 105
3.10. Genotyping of Antigenically Identical Organisms 105
3.11. Identifying New Infectious Agents 107
3.12. Simultaneously Detecting Multiple Agents 109
3.13. Quantitating Infectious Agents 110
4. Problems with Polymerase Chain Reaction Testing 110
5. Conclusions 112
References 112
8. Identifying Novel Bacteria Using a Broad Range
Polymerase Chain Reaction
BURT ANDERSON
1. Introduction 117
2. Theoretical Basis of Broad Range PCR 118
2.1. The 16S rRNA Gene Is Highly Conserved 118
2.2. The 16S rRNA as a Molecular Chronometer 119
2.3. Universal Primers One Primer Pair Fits All 119
2.4. Bacterial Identification without Isolation 120
3. Applications of Broad Range PCR 122
3.1. Ehrlichiosis 122
3.2. Granulotropic Ehrlichiosis 122
3.3. Whipple s Disease 124
3.4. Bacillary Angiomatosis/Cat Scratch Disease 124
CONTENTS xxi
4. Future Directions 125
4.1. Pathogens versus Normal Flora 125
4.2. Crohn s Disease 126
4.3. Sarcoidosis and Autoimmune Disease 126
4.4. Culture Negative Endocarditis 126
5. Conclusions 127
References 127
9. The Molecular Epidemiology of Nosocomial Infection:
An Overview of Principles, Application,
and Interpretation
RICHARD V. GOERING
1. Introduction 131
2. The Birth of Molecular Epidemiology 132
3. Chromosomal DNA: The Ultimate Molecule
of Epidemiological Comparison 132
4. Molecular Epidemiological Applications 135
4.1. Infection in the Individual Patient 137
4.2. Infection in Multiple Patients 138
5. The Interpretation of Molecular Typing Data 139
5.1. Principles 139
5.2. Chromosomal Changes as an Indicator
of Epidemiological Relatedness 140
6. Summary and Conclusions 145
References 147
10. Tyramide Signal Amplification: Applications
in Detecting Infectious Agents
G. J. LITT and M. N. BOBROYV
1. Introduction 159
2. Background and Terminology 160
3. Principles 160
3.1. Basis 160
3.2. Formats 163
xxii CONTENTS
4. Applications 165
5. Discussion 171
References 172
11. Ribotyping as a Tool for Molecular Epidemiology
SUSAN B. HUNTER and BALA SWAMINATHAN
1. Introduction 175
2. Ribotyping—the Method 176
3. Probes and Labels Used for Ribotyping 178
4. Ribotyping Applications in Molecular Epidemiology 178
5. Use of Multiple Restriction Enzymes 180
6. Comparison of Ribotyping with Other Molecular
Subtyping Methods 181
7. Polymerase Chain Reaction Ribotyping 184
8. Automation of Ribotyping 184
9. Other Applications of Ribotyping 185
10. Summary 185
References 186
12. Utility of PCR in Situ for Detecting Viral Infections
GERARD J.NUOVO
1. Introduction 191
2. The Key Preparatory Steps 192
2.1. Definition of Terms 192
2.2. The Fixative 192
2.3. The Glass Slide 193
2.4. The Protease Digestion Step 194
2.5. The Hot Start Maneuver 194
2.6. DNase Digestion 196
2.7. Determination of the Optimal Protease Digestion Time
for RT in Situ PCR 199
3. Protocols 200
3.1. PCR in Situ Hybridization 200
3.2. RT in Situ PCR 201
CONTENTS xxiii
3.3. Troubleshooting Guide 202
4. HIV 1 as a Model for PCR in Situ Hybridization and RT
in Situ PCR 203
References 206
Index 207
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| spelling | Rapid detection of infectious agents ed. by Steven Specter ... New York [u.a.] Plenum Press 1998 XXIII, 216 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Infectious agents and pathogenesis Diagnostics moléculaires Microbiologie clinique Pathogenic microorganisms - Detection Diagnostic microbiology Genetic Techniques Immunologic Techniques Infection diagnosis Molecular diagnosis Polymerase Chain Reaction Virus Diseases diagnosis Pathogener Mikroorganismus (DE-588)4044890-3 gnd rswk-swf Diagnostik (DE-588)4113303-1 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Pathogener Mikroorganismus (DE-588)4044890-3 s Diagnostik (DE-588)4113303-1 s DE-604 Specter, Steven Sonstige oth HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008460502&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Rapid detection of infectious agents Diagnostics moléculaires Microbiologie clinique Pathogenic microorganisms - Detection Diagnostic microbiology Genetic Techniques Immunologic Techniques Infection diagnosis Molecular diagnosis Polymerase Chain Reaction Virus Diseases diagnosis Pathogener Mikroorganismus (DE-588)4044890-3 gnd Diagnostik (DE-588)4113303-1 gnd |
| subject_GND | (DE-588)4044890-3 (DE-588)4113303-1 (DE-588)4143413-4 |
| title | Rapid detection of infectious agents |
| title_auth | Rapid detection of infectious agents |
| title_exact_search | Rapid detection of infectious agents |
| title_full | Rapid detection of infectious agents ed. by Steven Specter ... |
| title_fullStr | Rapid detection of infectious agents ed. by Steven Specter ... |
| title_full_unstemmed | Rapid detection of infectious agents ed. by Steven Specter ... |
| title_short | Rapid detection of infectious agents |
| title_sort | rapid detection of infectious agents |
| topic | Diagnostics moléculaires Microbiologie clinique Pathogenic microorganisms - Detection Diagnostic microbiology Genetic Techniques Immunologic Techniques Infection diagnosis Molecular diagnosis Polymerase Chain Reaction Virus Diseases diagnosis Pathogener Mikroorganismus (DE-588)4044890-3 gnd Diagnostik (DE-588)4113303-1 gnd |
| topic_facet | Diagnostics moléculaires Microbiologie clinique Pathogenic microorganisms - Detection Diagnostic microbiology Genetic Techniques Immunologic Techniques Infection diagnosis Molecular diagnosis Polymerase Chain Reaction Virus Diseases diagnosis Pathogener Mikroorganismus Diagnostik Aufsatzsammlung |
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