Mitosis and meiosis:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
San Diego [u.a.]
Acad. Press
1999
|
| Series: | Methods in cell biology
61 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XV, 489 S. Ill., graph. Darst. |
| ISBN: | 0125441630 0125885407 |
Staff View
MARC
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Record in the Search Index
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CONTENTS
Contributors xi
Preface xv
1. Isolation of Centrosomes from Drosophila Embryos
Michelle Moritz and Bruce M. Alberts
I. Introduction 1
II. Isolation of Centrosomes from Drosophila Embryos 2
III. Assays for Microtubule Nucleation by Isolated Centrosomes 5
IV. Conclusions 11
References 11
2. Studying the Composition and Function of Centrosomes in Vertebrates
Michel Bomens and Mohammed Moudjou
I. Introduction 14
II. Isolation of Centrosomes from Animal Cells 15
III. Preparation of Immunological Probes from Isolated Centrosomes 18
IV. Ultrastructural Analysis of Isolated Centrosomes 20
V. Biochemical Composition of Centrosomes 24
VI. Functional Assays of Isolated Centrosomes 27
VII. Prospects 29
References 31
3. Isolation of Centrosomes from Spisula solidissima Oocytes
Robert E. Palazzo and Jacalyn M. Vogel
I. Introduction 36
II. Obtaining Organisms 37
III. Isolation and Activation of Spisula solidissima Oocytes 37
IV. Preparation of Oocyte Lysates 41
V. Preparation of Microtubule Protein 43
VI. Isolation of Centrosomes from Activated Oocyte Lysates 45
VII. Immunofluorescence of Centrosomes and Asters 49
VIII. Electron Microscopy of Asters and Centrosomes 52
IX. Summary 53
References 56
Contents
4. Methods for in Situ Localization of Proteins and DNA in the
Centromere—Kinetochore Complex
A. Van Hooser and B. R. Brinkley
I. Introduction 57
II. In Situ Localization of Proteins: Indirect Immunofluorescence 59
III. In Situ Localization of Proteins: Immunogold EM 63
IV. Fluorescent in Situ Hybridization Using DNA Satellite Probes 66
V. Combination Staining: DNA/Protein 69
VI. Specialized Techniques 72
References 78
5. Three Dimensional Transmission Electron Microscopy and Its Application
to Mitosis Research
Bruce F. McEwen and Michael Marko
I. Introduction 82
II. Resolution and Choosing between Tomography and Serial Sections 84
III. Electron Tomography 87
IV. Serial Section Reconstruction 98
V. Analysis and Display of 3D Reconstructions 99
VI. Software Packages 105
VII. Summary and Conclusions 106
References 108
6. Enlightening Mitosis: Construction and Expression of Green Fluorescent
Protein Fusion Proteins
Kevin F. Sullivan
I. Introduction: Visualizing the Molecular Anatomy of the Spindle 114
II. Fluorescence Properties of GFP 114
III. Strategies for Constructing Fusion Proteins 118
IV. Expression in Mammalian Cells 123
References 133
7. Recombinant p50/Dynamitin as a Tool to Examine the Role of Dynactin
in Intracellular Processes
Torsten Wittmann and Tony Hyman
I. Introduction 137
II. Production of Recombinant p50/Dynamitin 138
III. Disruption of the Dynactin Complex by p50/Dynamitin in Xenopus Egg
Extracts 140
IV. Disruption of Spindle Poles Using p50/Dynamitin 142
References 142
Contents vii
8. In Vitro Assays for Studying Saccharomyces cerevisiae Kinetochore Activity
Fedor Severin, Ken Kaplan, Peter Sorger, and Tony Hyman
I. Introduction 145
II. Microtubule Binding Assays for S. cerevisiae Kinetochores 146
III. Band Shift Assay for the Kinetochore Complex 151
References 153
9. Fluorescent Speckle Microscopy of Spindle Microtubule Assembly and
Motility in Living Cells
Clare Waterman Storer, Arshad Desai, and E. D. Salmon
I. Introduction 156
II. Principles of the Fluorescence Speckle Method for Microtubules 156
III. Specimen Methods 158
IV. Microscopy and Image Acquisition 161
V. Image Processing and Analysis 164
VI. Examples 167
VII. Future Considerations 170
References 172
10. Polarized Light Microscopy of Spindles
Rudolf Olienbourg
I. Introduction 175
II. Polarized Light Microscopy 178
III. Analysis of Spindle Birefringence 190
IV. Optimum Cell Types for Polarized Light Microscopy of Spindles 197
References 206
11. Micromanipulation of Chromosomes and Spindles in Insect Spermatocytes
Dahong Zhang and R. Bruce Nicklas
I. Introduction 209
II. Preparing for Micromanipulation 210
III. Manipulating Cell Components 216
References 217
12. Microinjection of Mitotic Cells
Patricia Wadsworth
I. Introduction 219
II. Choice of Cells 221
III. Timing of Injection 223
IV. Microinjection Procedure 223
V. Conclusions 228
References 229
[ Contents
13. Obtaining Antibodies to Spindle Components
Ryoko Kuriyama and Kathy Ensrud
I. Introduction 233
II. Methods 235
III. Discussion 243
References 244
14. Using Antisense Technology to Study Mitosis
Linda Wordeman and Mike Wagenbach
I. Introduction 245
II. Antisense Mechanism of Action 247
III. Choice of Antisense Reagents 248
IV. Assaying Target Protein Levels 254
V. Antisense Reagents Used to Study Cell Division 255
References 261
15. The Use and Action of Drugs in Analyzing Mitosis
Mary Ann Jordan and Leslie Wilson
I. Introduction: Why Use Drugs? 267
II. Brief Overview of Microtuhule Assembly Dynamics 268
III. Mechanisms of Action of Major Antimitotic Drugs: Binding to Tubulin and
Microtubules and Effects on Microtubule Polymerization and Dynamics and
on Mitosis 270
IV. Determination of Intracellular Drug Levels 287
V. How to Use Antimitotic Drugs: Practical Guidelines 288
References 289
16. Correlative Light and Electron Microscopy of Mitotic Cells in
Monolayer Cultures
Conly L. Rieder and Grisel Cassels
I. Introduction 297
II. Light Microscopy 299
III. Flat Embedding 302
IV. Preparing the Cell for Sectioning 305
V. Obtaining the Required Ultrastructural Information 307
References 313
17. Identification and Characterization of Mitotic Mutations in Drosophila
William E. Theurkauf and Margarete M. S. Heck
I. Introduction 318
II. Maternal Effect Mutations That Disrupt the Syncytial Mitotic Divisions 322
HI. Cytological Analysis of the Syncytial Mitoses 324
Contents ix
IV. Zygotic Mutations That Disrupt Mitosis in Larval Tissues 334
V. Cytological Analysis of Larval Brain and Imaginal Discs 337
References 344
18. Methods for Isolating and Analyzing Mitotic Mutants in Aspergillus nidulans
Berl R. Oakley
I. Introduction 348
II. Characteristics of Aspergillus nidulans 348
III. Strains and Media 353
IV. Harvesting Conidia and Preparing Conidial Suspensions 354
V. Mutagenesis 355
VI. Methods for Isolating Mitotic Mutants in A. nidulans 355
VII. Morphological Analysis of Mutants 361
VIII. Genetic Analysis of Mutants 363
IX. Molecular Genetic Methods for Working with A. nidulans 365
References 367
19. Using Green Fluorescent Protein Fusion Proteins to Quantitate Microtubule
and Spindle Dynamics in Budding Yeast
Kerry Bloom, Dale L. Beach, Paul Maddox, Sidney L. Shaw, Elaine Yeh,
and E. D. Salmon
I. Introduction 369
II. Construction of Protein GFP Fusion and Promoter Selection 370
III. Quantifying Fluorescence in Cell Populations 373
IV. The Imaging System 375
V. Quantitative Solution to the Imaging Problem 376
VI. Image Acquisition and Processing 378
VII. Applications and Examples: Expression of Dynein GFP in Vivo 379
References 381
20. The Use of Xenopus Egg Extracts to Study Mitotic Spindle Assembly and
Function in Vitro
Arshad Desai, Andrew Murray, Timothy J. Mitchison, and Claire E. Walczak
I. Introduction 386
II. Preparation of CSF Extracts for Spindle Assembly 387
III. Spindle Assembly Reactions 393
IV. Monitoring Spindle Assembly Reactions 397
V. Manipulation of Extracts 401
VI. Data Analysis and Interpretation 404
VII. Anaphase in Vitro 405
VIII. Conclusions 410
References 411
Contents
21. Methods for Studying Cell Division in Higher Plants
Jan W. Vos, Aline H. Valster, and Peter K. Hepler
I. Introduction 413
II. Cell Types of the Study of Cell Division 414
HI. Microinjection of Plant CeUs 428
IV. Conclusions 433
References 434
22. Using Sea Urchin Gametes for the Study of Mitosis
Greenfield Sluder, Frederick J. Miller, and Edward H. Hinchcliffe
I. Introduction 440
II. The Experimental System 440
III. Maintenance of the Organisms 441
IV. Obtaining Gametes 443
V. Zygotes 444
VI. Mounting Cells for Observation 448
VII. Other Methods 462
VIII. Annotated List of References 464
References 472
Index 473 |
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| spelling | Mitosis and meiosis ed. by Conly L. Rieder San Diego [u.a.] Acad. Press 1999 XV, 489 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 61 Celdeling gtt Citologia larpcal Meiose gtt Mitose Méiose Cell Division Cell Fractionation methods Cell division Meiosis Mitosis Mitose (DE-588)4170185-9 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Cytologie (DE-588)4070177-3 gnd rswk-swf Meiose (DE-588)4169347-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Meiose (DE-588)4169347-4 s Cytologie (DE-588)4070177-3 s Methode (DE-588)4038971-6 s DE-604 Mitose (DE-588)4170185-9 s Rieder, Conly L. Sonstige oth Methods in cell biology 61 (DE-604)BV002534878 61 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008383843&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mitosis and meiosis Methods in cell biology Celdeling gtt Citologia larpcal Meiose gtt Mitose Méiose Cell Division Cell Fractionation methods Cell division Meiosis Mitosis Mitose (DE-588)4170185-9 gnd Methode (DE-588)4038971-6 gnd Cytologie (DE-588)4070177-3 gnd Meiose (DE-588)4169347-4 gnd |
| subject_GND | (DE-588)4170185-9 (DE-588)4038971-6 (DE-588)4070177-3 (DE-588)4169347-4 (DE-588)4143413-4 |
| title | Mitosis and meiosis |
| title_auth | Mitosis and meiosis |
| title_exact_search | Mitosis and meiosis |
| title_full | Mitosis and meiosis ed. by Conly L. Rieder |
| title_fullStr | Mitosis and meiosis ed. by Conly L. Rieder |
| title_full_unstemmed | Mitosis and meiosis ed. by Conly L. Rieder |
| title_short | Mitosis and meiosis |
| title_sort | mitosis and meiosis |
| topic | Celdeling gtt Citologia larpcal Meiose gtt Mitose Méiose Cell Division Cell Fractionation methods Cell division Meiosis Mitosis Mitose (DE-588)4170185-9 gnd Methode (DE-588)4038971-6 gnd Cytologie (DE-588)4070177-3 gnd Meiose (DE-588)4169347-4 gnd |
| topic_facet | Celdeling Citologia Meiose Mitose Méiose Cell Division Cell Fractionation methods Cell division Meiosis Mitosis Methode Cytologie Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008383843&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV002534878 |
| work_keys_str_mv | AT riederconlyl mitosisandmeiosis |