Genkarten-orientierte Isolierung des Mlo-Gens aus Gerste (Hordeum vulgare):
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
1997
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Aachen, Techn. Hochschule, Diss., 1997 |
| Beschreibung: | 115 S. graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV012309134 | ||
| 003 | DE-604 | ||
| 005 | 19990720 | ||
| 007 | t | ||
| 008 | 981209s1997 d||| m||| 00||| engod | ||
| 016 | 7 | |a 954611136 |2 DE-101 | |
| 035 | |a (OCoLC)57480195 | ||
| 035 | |a (DE-599)BVBBV012309134 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-M49 |a DE-355 |a DE-11 | ||
| 084 | |a LAN 220d |2 stub | ||
| 084 | |a LAN 311d |2 stub | ||
| 100 | 1 | |a Büschges, Rainer |d 1963- |e Verfasser |0 (DE-588)12047381X |4 aut | |
| 245 | 1 | 0 | |a Genkarten-orientierte Isolierung des Mlo-Gens aus Gerste (Hordeum vulgare) |c vorgelegt von Rainer Büschges |
| 264 | 1 | |c 1997 | |
| 300 | |a 115 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Aachen, Techn. Hochschule, Diss., 1997 | ||
| 650 | 0 | 7 | |a Resistenzgen |0 (DE-588)4280023-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Gerstenmehltau |0 (DE-588)4273546-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Genklonierung |0 (DE-588)4123275-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Gerste |0 (DE-588)4156898-9 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Gerste |0 (DE-588)4156898-9 |D s |
| 689 | 0 | 1 | |a Gerstenmehltau |0 (DE-588)4273546-4 |D s |
| 689 | 0 | 2 | |a Resistenzgen |0 (DE-588)4280023-7 |D s |
| 689 | 0 | 3 | |a Genklonierung |0 (DE-588)4123275-6 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008344397&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-008344397 | |
Datensatz im Suchindex
| _version_ | 1807501325159628800 |
|---|---|
| adam_text |
CONTENTS
1
INTRODUCTION
1
1.1
DISEASE
RESISTANCE
IN
PLANTS
1
11.1
PREFORMED
BARRIERS
2
1
1.2
INDUCIBLE
MECHANISMS
3
1
1.3
ENGINEERING
OF
DISEASE
RESISTANCE
4
1.14
GENETIC
BASIS
OF
PLANT/PATHOGEN
INTERACTIONS
5
1.2
ROUTES
TOWARDS
THE
ISOLATION
OF
PLANT
RESISTANCE
GENES
6
12.1
GENE
ISOLATION
BASED
ON
INSERTIONAL
MUTAGENESIS
7
1.2.2
ISOLATION
OF
PLANT
RESISTANCE
GENES
VIA
MAP-BASED
CLONING
7
1.2.2.
1
MOLECULAR
MARKERS,
LINKAGE
DETECTION
AND
FINE
MAPPING
8
1
2.2.2
GENE
ISOLATION
BY
CHROMOSOME
WALKING
11
1
2.2.3
IDENTIFICATION
AND
VERIFICATION
OF
A
CANDIDATE
GENE
12
1.3
STRUCTURE
AND
FUNCTION
OF
DISEASE
RESISTANCE
GENES
13
1.4
THE
BARLEY
MIO
GENE,
'
JUST
ANOTHER
'
R
GENE
?
15
1.5
OBJECTIVES
OF
THIS
THESIS
16
2
EXPERIMENTAL
PROCEDURES
18
2.1
BARLEY
CULTIVARS
AND
CROSSINGS
1*
2.2
POWDERY
MILDEW
INFECTION
TESTS
2.3
GENERAL
METHODS
AND
MATERIALS
2.4
AFLP ANALYSIS
21
2.4.1
PRINCIPLE
21
2.4
2
PREPARATION
OF
BARLEY
GENOMIC
DNA
22
2.4
3
AFLP
TEMPLATE
PREPARATION
22
2
4
4
AFLP
REACTIONS
23
2.4
5
GEL
ANALYSIS
23
2.5
ISOLATION
OF
YAC
ENDFRAGMENTS
23
2
5
1
ISOLATION
OF
GENOMIC
YAC
DNA
23
2
5
2
PREPARATION
OF
TEMPLATE
DNA
FOR
INVERSE
PCR
(IPCR)
25
2
5
3
INVERSE
PCR
(IPCR)
26
2
5.4
RESTRICTION
ANALYSIS,
PURIFICATION
AND
SEQUENCING
OF
CANDIDATE
26
FRAGMENTS
CONTENTS
2.6
BAC
F15
RANDOM
SEQUENCING
PROJECT
27
2.6.1
VERIFICATION
OF
AFLP
MARKERS
AND
YEF8
ON
THE
BAC
F
15
INSERT
27
2.6.2
ISOLATION
OF
E.COLI-DEMED
BAC
F15
PLASMID
DNA
28
2.6
3
SELECTION
FOR
SEQUENCING
CANDIDATE
CLONES
29
2.6.4
SEQUENCING
OF
BAC
F15-DERIVED
DNA
FRAGMENTS
30
2.7
DNA
SEQUENCE
ANALYSIS
OF
PLANT-DERIVED
PCR
PRODUCTS
30
2.8
STATISTICAL
FORMULAS
32
3
RESULTS
33
3.1
BACKGROUND
34
3.2
SATURATION
OF
AN
AFLP
MARKER
INTERVAL
(BPML/BPM9)
SURROUNDING
37
THE
MIO
LOCUS
WITH
76
RECOMBINATION
EVENTS
3.3
AFLP
FINE
MAPPING:
MARKER
BPML6
CO-SEGREGATES
WITH
MIO
BASED
43
ON
4,044
MEIOTIC
EVENTS
3.4
POWDERY
MILDEW
INOCULATION
EXPERIMENTS
CONFIRM
BPML6
AS
47
CO-SEGREGATING
MARKER
WITH
MIO
3.4.1
SEQUENCING
OF
BPM2,
BPM9
AND
BPML6
48
3
4.2
SCREENING
OF
A
BARLEY
YAC
LIBRARY
USING
BPML
6
AS
A
PROBE
50
REVEALED
FOUR
MO-CONTAINING
YACS
3.4.3
THE
60
KB
BAC
CLONE
F15,
DERIVED
FROM
YAC
YHV303-A6,
50
HARBOURS
MIO
3
.4
4
SCREENING
OF
A
BARLEY
CDNA
LIBRARY
REVEALED
THE
CLONE
C7
51
USING
BPML
6
AS
A
PROBE
3.5
COMPARATIVE
SEQUENCE
ANALYSIS
USING
C7
INSERT
DNA,
DERIVED
FROM
52
VARIOUS
CULTIVARS,
REVEALS
NO
POLYMORPHISMS
APART
FROM
THE
BPML6-CAUSING
EVENT
3.6
LOCALISATION
OF
MIO
ON
THREE
INDEPENDENT
CLONES
OF
A
BARLEY
54
YAC
LIBRARY
3.6.1
ISOLATION
OF
YAC
YHV303-A6
ENDFRAGMENTS
56
3.7
PHYSICAL
DELIMITATION
OF
MIO
ON
THE
YHV303-A6-DERIVED
59
BAC
FL
5
INSERT
3.8
PCR-BASED
SEQUENCING
PROJECT
USING
BAC
F15
DNA
59
3.9
SEQUENCE
CONTIG
25,
CARRYING
THE
C7
INSERT,
DISPLAYS
SIGNIFICANTLY
66
HIGH
CODING
PROBABILITY
CONTENTS
3.10
IDENTIFICATION
OF
A
CANDIDATE
MIO
GENE
68
3.11
PCR-BASED
SEQUENCING
OF
THE
CANDIDATE
MIO
GENE,
USING
DIVERSE
69
M/O-MUTANTS
AS
TEMPLATES,
REVEALED
NOVEL
INDEPENDENT,
SINGLE
MUTATION
EVENTS
IN
THE
5
'
REGION
OF
THE
GENE
3.12
AUTHENTICITY
OF
THE
MIO
GENE
WAS
CONFIRMED
BY
COMPARATIVE
73
SEQUENCE
ANALYSIS
OF
INTRAGENIC
RECOMBINANTS
4
DISCUSSION
77
4.1
CHROMOSOME
LANDING
IN
A
COMPLEX
GENOME
:
HIGH
VOLUME
AFLP
77
MARKER
TECHNOLOGY
ENABLES
POSITIONAL
CLONING
OF
THE
BARLEY
MIO
GENE
4.2
MUTANTS
AND
INTRAGENIC
RECOMBINANTS:
SUITABLE
TOOLS
FOR
GENE
82
IDENTIFICATION
4.3
MIO
-
A
MEMBER
OF
A
NOVEL
CLASS
OF
PLANT
GENES
INVOLVED
IN
86
DISEASE
RESISTANCE
CONTROL
4.4
MIO
-
A
PROTEIN
WITH
A
DUAL
CONTROL
FUNCTION
90
5
SUMMARY
96
6
REFERENCES
97
7
APPENDIX
113
7.1
INDEX
OF
ABBREVIATIONS
113
7.2
INDEX
OF
FIGURES
114
7.3 INDEX
OF
TABLES
115 |
| any_adam_object | 1 |
| author | Büschges, Rainer 1963- |
| author_GND | (DE-588)12047381X |
| author_facet | Büschges, Rainer 1963- |
| author_role | aut |
| author_sort | Büschges, Rainer 1963- |
| author_variant | r b rb |
| building | Verbundindex |
| bvnumber | BV012309134 |
| classification_tum | LAN 220d LAN 311d |
| ctrlnum | (OCoLC)57480195 (DE-599)BVBBV012309134 |
| discipline | Agrarwissenschaft Pflanzenbau |
| format | Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV012309134 |
| illustrated | Illustrated |
| indexdate | 2024-08-16T00:19:48Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008344397 |
| oclc_num | 57480195 |
| open_access_boolean | |
| owner | DE-M49 DE-BY-TUM DE-355 DE-BY-UBR DE-11 |
| owner_facet | DE-M49 DE-BY-TUM DE-355 DE-BY-UBR DE-11 |
| physical | 115 S. graph. Darst. |
| publishDate | 1997 |
| publishDateSearch | 1997 |
| publishDateSort | 1997 |
| record_format | marc |
| spelling | Büschges, Rainer 1963- Verfasser (DE-588)12047381X aut Genkarten-orientierte Isolierung des Mlo-Gens aus Gerste (Hordeum vulgare) vorgelegt von Rainer Büschges 1997 115 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Aachen, Techn. Hochschule, Diss., 1997 Resistenzgen (DE-588)4280023-7 gnd rswk-swf Gerstenmehltau (DE-588)4273546-4 gnd rswk-swf Genklonierung (DE-588)4123275-6 gnd rswk-swf Gerste (DE-588)4156898-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Gerste (DE-588)4156898-9 s Gerstenmehltau (DE-588)4273546-4 s Resistenzgen (DE-588)4280023-7 s Genklonierung (DE-588)4123275-6 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008344397&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Büschges, Rainer 1963- Genkarten-orientierte Isolierung des Mlo-Gens aus Gerste (Hordeum vulgare) Resistenzgen (DE-588)4280023-7 gnd Gerstenmehltau (DE-588)4273546-4 gnd Genklonierung (DE-588)4123275-6 gnd Gerste (DE-588)4156898-9 gnd |
| subject_GND | (DE-588)4280023-7 (DE-588)4273546-4 (DE-588)4123275-6 (DE-588)4156898-9 (DE-588)4113937-9 |
| title | Genkarten-orientierte Isolierung des Mlo-Gens aus Gerste (Hordeum vulgare) |
| title_auth | Genkarten-orientierte Isolierung des Mlo-Gens aus Gerste (Hordeum vulgare) |
| title_exact_search | Genkarten-orientierte Isolierung des Mlo-Gens aus Gerste (Hordeum vulgare) |
| title_full | Genkarten-orientierte Isolierung des Mlo-Gens aus Gerste (Hordeum vulgare) vorgelegt von Rainer Büschges |
| title_fullStr | Genkarten-orientierte Isolierung des Mlo-Gens aus Gerste (Hordeum vulgare) vorgelegt von Rainer Büschges |
| title_full_unstemmed | Genkarten-orientierte Isolierung des Mlo-Gens aus Gerste (Hordeum vulgare) vorgelegt von Rainer Büschges |
| title_short | Genkarten-orientierte Isolierung des Mlo-Gens aus Gerste (Hordeum vulgare) |
| title_sort | genkarten orientierte isolierung des mlo gens aus gerste hordeum vulgare |
| topic | Resistenzgen (DE-588)4280023-7 gnd Gerstenmehltau (DE-588)4273546-4 gnd Genklonierung (DE-588)4123275-6 gnd Gerste (DE-588)4156898-9 gnd |
| topic_facet | Resistenzgen Gerstenmehltau Genklonierung Gerste Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008344397&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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