Mycobacteria protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1998
|
| Schriftenreihe: | Methods in molecular biology
101 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIV, 472 S. Ill., graph. Darst. |
| ISBN: | 0896034712 |
Internformat
MARC
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| 245 | 1 | 0 | |a Mycobacteria protocols |c ed. by Tanya Parish ... |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 1998 | |
| 300 | |a XIV, 472 S. |b Ill., graph. Darst. | ||
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| 490 | 1 | |a Methods in molecular biology |v 101 | |
| 650 | 4 | |a Bacterial genetics |v Laboratory manuals | |
| 650 | 4 | |a DNA |x physiology | |
| 650 | 4 | |a Molecular Biology |x methods | |
| 650 | 4 | |a Mycobacteria |v Laboratory manuals | |
| 650 | 4 | |a Mycobacterium |x genetics | |
| 650 | 4 | |a Mycobacterium |x growth & development | |
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Datensatz im Suchindex
| _version_ | 1804126868017774592 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
1 Mycobacteria: Bugs and Bugbears
Tanya Parish and Neil G. Stoker 1
2 Mycobacteria: General Culture Methodology and Safety
Considerations
Bryan W.Allen 15
3 Isolation of Genomic DNA from Mycobacteria
John T. Belisle and Michael G. Sonnenberg 31
4 Nucleic Acid Extraction: Plasmid/Cosmid DNA
Bonnie B. Plikaytis and C. Harold King 45
5 Pulsed Field Gel Electrophoresis for Mycobacteria
Wolfgang J. Philipp,Stephen Gordon, Amalio Telenti, and
Stewart T. Cole 51
6 Extraction of RNA from Mycobacteria
Eshwar Mahenthiralingam 65
7 Preparation of Cell Free Extracts from Mycobacteria
Tanya Parish and Paul R. Wheeler 77
8 Preparation of Cell Wall Fractions from Mycobacteria
Gurdyal S. Besra 91
9 Using MycDB on the World Wide Web
Staffan Bergh and Stewart T. Cole 109
10 Conjugating DNA into Mycobacteria
Julie Ann Gavigan and Carlos Martin 119
11 Electroporation of Mycobacteria
Tanya Parish and Neil G. Stoker 129
12 Mycobacteriophages
GaryJ. Sarkis and Graham F. Hatfull 145
viii Contents
13 Chemical Mutagenesis of Mycobacteria
Lucy A. Brooks 175
14 Tn671 Transposon Mutagenesis in Mycobacterium smegmatis
Using a Temperature Sensitive Delivery System
Esther Perez, Julie Ann Gavigan, Isabel Otal,
Christophe Guilhot, Vladimir Pelicic, Brigitte Giguel,
and Carlos Martin 187
15 Homologous Recombination in Mycobacterium smegmatis:
Screening Methods for Detection of Gene Replacement
Robert N. Husson 199
16 Gene Replacement in Mycobacterium smegmatis Using a
Dominant Negative Selectable Marker
Peter Sander and Erik C. Bottger 207
17 Gene Replacement in Mycobacterium intracellulare
Britt lnger Marklund and Richard W. Stokes 217
18 Gene Replacement in Mycobacterium bovis BCG
Elizabeth Norman 225
19 The Application of Bacterial Luciferase as a Reporter Gene
in Mycobacteria
Stephen Gordon, Gavin Chung, and Peter Andrew 235
20 Green Fluorescent Protein: A Tool for Gene Expression and
Cell Biology in Mycobacteria
Laura E. Via, Subramanian Dhandayuthapani,
Dusanka Deretic, and V. Deretic 245
21 Expression of Genes in Mycobacteria
Peadar 6 Gaora 261
22 Secretion of Mammalian Proteins from Mycobacteria
Peter J. Murray and Richard A. Young 275
23 Intracellular Gene Expression: Analysis of RNA from Mycobacteria
in Macrophages Using RT PCR
Philip D. Butcher, Joseph A. Mangan, and Irene M. Monahan.... 285
24 Analysis of Mycobacterial Differential Gene Expression
by RAP PCR
Joseph A. Mangan and Philip D. Butcher 307
25 Mycobacterial Speciation
Francis A. Drobniewski and Anne H. C. Uttley 323
26 Species Identification of Mycobacteria Using rDNA Sequencing
Philip Kirschnerand Erik C. Bottger 349
Contents jx
27 Detection of Mycobacterium tuberculosis by a Colorimetric
Polymerase Chain Reaction
Stuart M. Wilson 363
28 Rapid Detection and Simultaneous Strain Differentiation of
Mycobacterium tuberculosis Complex Bacteria by Spoligotyping
Henri O. F. Molhuizen, Annelies E. Bunschoten, Leo M. Schouls,
and Jan D. A. van Embden 381
29 Computer Analysis of IS6710 RFLP Patterns of Mycobacterium
tuberculosis
Herre F. Heersma, Kristin Kremer, and Jan D. A. van Embden... 395
30 Detection of Mutations in Mycobacteria by PCR SSCP
(Single Strand Conformation Polymorphism)
Amalio Telenti, Nadine Honore, and Stewart T. Cole 423
31 The Use of Luciferase Reporter Phage for Antibody Susceptibility
Testing of Mycobacteria
Paul F. Riska and William R. Jacobs, Jr. 431
Index 457
|
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| id | DE-604.BV012251248 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:24:17Z |
| institution | BVB |
| isbn | 0896034712 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008301266 |
| oclc_num | 246123624 |
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| owner_facet | DE-355 DE-BY-UBR DE-12 |
| physical | XIV, 472 S. Ill., graph. Darst. |
| publishDate | 1998 |
| publishDateSearch | 1998 |
| publishDateSort | 1998 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Mycobacteria protocols ed. by Tanya Parish ... Totowa, NJ Humana Press 1998 XIV, 472 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 101 Bacterial genetics Laboratory manuals DNA physiology Molecular Biology methods Mycobacteria Laboratory manuals Mycobacterium genetics Mycobacterium growth & development Methode (DE-588)4038971-6 gnd rswk-swf Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Mykobakterien (DE-588)4170927-5 gnd rswk-swf Mykobakterien (DE-588)4170927-5 s Molekulargenetik (DE-588)4039987-4 s Methode (DE-588)4038971-6 s DE-604 Parish, Tanya Sonstige oth Methods in molecular biology 101 (DE-604)BV035362695 101 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008301266&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mycobacteria protocols Methods in molecular biology Bacterial genetics Laboratory manuals DNA physiology Molecular Biology methods Mycobacteria Laboratory manuals Mycobacterium genetics Mycobacterium growth & development Methode (DE-588)4038971-6 gnd Molekulargenetik (DE-588)4039987-4 gnd Mykobakterien (DE-588)4170927-5 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4039987-4 (DE-588)4170927-5 |
| title | Mycobacteria protocols |
| title_auth | Mycobacteria protocols |
| title_exact_search | Mycobacteria protocols |
| title_full | Mycobacteria protocols ed. by Tanya Parish ... |
| title_fullStr | Mycobacteria protocols ed. by Tanya Parish ... |
| title_full_unstemmed | Mycobacteria protocols ed. by Tanya Parish ... |
| title_short | Mycobacteria protocols |
| title_sort | mycobacteria protocols |
| topic | Bacterial genetics Laboratory manuals DNA physiology Molecular Biology methods Mycobacteria Laboratory manuals Mycobacterium genetics Mycobacterium growth & development Methode (DE-588)4038971-6 gnd Molekulargenetik (DE-588)4039987-4 gnd Mykobakterien (DE-588)4170927-5 gnd |
| topic_facet | Bacterial genetics Laboratory manuals DNA physiology Molecular Biology methods Mycobacteria Laboratory manuals Mycobacterium genetics Mycobacterium growth & development Methode Molekulargenetik Mykobakterien |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008301266&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT parishtanya mycobacteriaprotocols |