CRC handbook of viruses: mass-molecular weight values and related properties
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| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Boca Raton [u.a.]
CRC Press
1998
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XIII, 206 S. Ill. |
| ISBN: | 0849326257 |
Staff View
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| 245 | 1 | 0 | |a CRC handbook of viruses |b mass-molecular weight values and related properties |c Horace M. Mazzone |
| 264 | 1 | |a Boca Raton [u.a.] |b CRC Press |c 1998 | |
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| 650 | 4 | |a Virology |x instrumentation | |
| 650 | 4 | |a Viruses |v Handbooks, manuals, etc | |
| 650 | 4 | |a Viruses |x chemistry | |
| 650 | 4 | |a Viruses |x isolation & purification | |
| 650 | 4 | |a Viruses |x ultrastructure | |
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Record in the Search Index
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|---|---|
| adam_text | Contents
1 Introduction 1
1.1 The Attraction of Physicists and Chemists to Biology 9
2 Viruses as Macromolecules 15
2.1 The Macromolecule Concept 15
2.2 Proteins 22
2.3 Protein Structure 25
2.4 Nucleic Acids 32
2.5 Nucleic Acid Structure 36
2.5.1 Denaturation of Double Stranded Nucleic Acids 38
2.6 Viruses as Nucleoproteins 39
2.7 Physical Principles in the Construction of Regular Viruses 41
3 Purification Considerations 45
3.1 Differential Centrifugation 45
3.1.1 Zonal Centrifugation 45
3.1.1.1 Rate Zonal Centrifugation 45
3.1.1.2 Isopycnic Centrifugation 47
3.1.1.3 Centrifugation with Zonal Rotors 49
3.1.2 Sedimentation Field Flow Fractionation 54
4 Crystallography 57
5 Molecular Weight Determinations from Sedimentation Experiments 69
5.1 Analytical Ultracentrifugation 69
5.1.1 Instrumentation 69
5.1.1.1 Rotors 69
5.1.1.2 Cells and Avoidance of Convection 72
5.1.2 Optical Systems 73
5.1.2.1 Absorption Optics 73
5.1.2.2 The Schlieren Optical System 75
5.1.2.3 The Rayleigh Interference Optical System 77
5.1.3 Analyzing the Optical Record 79
5.1.3.1 Manual Techniques for Schlieren Patterns 79
5.1.3.2 Comparator Readings for Interference Patterns 81
5.1.3.3 Determination of Vertical Spacing Between Fringes 82
5.1.3.4 Measurements of Absorption Records 83
5.1.3.5 Measurements on Traces from the Photoelectric Scanner 83
5.1.3.6 Off Line Analysis 84
5.1.3.7 Online Analysis 84
xi
5.1.4 Determination of Concentration and Spectrophotometric Parameters 85
5.1.5 Measurement of Areas of Schlieren Peaks 86
5.1.6 Interconversion of Schlieren and Interference Fringe Concentrations 87
5.1.7 Sedimentation Velocity 88
5.1.7.1 Sedimentation Theory 88
5.1.7.2 Determination of Sedimentation Coefficients 90
5.1.8 Diffusion Coefficient 95
5.1.8.1 Diffusion 95
5.1.8.2 Measurement of D by Dynamic Light Scattering (DLS) 97
5.1.8.3 Alternative Procedures for Obtaining D and S: DLS Applied to
Density Gradients 100
5.1.9 Sedimentation Equilibrium 101
5.1.9.1 Theory 101
5.1.9.2 Conventional Sedimentation Equilibrium Method 104
5.1.9.3 Meniscus Depletion Sedimentation Equilibrium Method 108
5.1.9.4 Short Column Sedimentation Equilibrium Method 109
5.1.9.5 Approach to Equilibrium Method 112
5.2 Density Gradient Centrifugation 115
5.2.1 Stabilized Moving Boundary Centrifugation 116
5.2.2 Zone Centrifugation 117
5.2.3 Isopycnic Gradient Centrifugation 118
5.2.4 Use of Zonal Rotors 119
5.3 Supplementary Procedures for Obtaining Molecular Weight from Values of Density,
the Partial Specific Volume, Viscosity, and the Frictional Coefficient 121
5.3.1 Density 121
5.3.2 Partial Specific Volume, v 121
5.3.3 Viscosity 123
5.3.4 The Molar Frictional Coefficient 124
5.4 Empirical Equations Relating Molecular Weight and the Corrected
Sedimentation Coefficient 126
5.5 Sedimentation Field Flow Fractionation 127
5.5.1 Retention Theory 127
5.5.2 Effective Molecular Weight 128
5.5.3 Molecular Weight and Density 128
5.6 Obtaining the Molecular Weight by Combining Sedimentation Experiments
with Other Technologies 128
6 Mass Molecular Weight Determinations from Scattering Studies 131
6.1 Small Angle X Ray Scattering (SAXS) 131
6.1.1 Theory 131
6.1.1.1 Instrumental Considerations 132
6.1.1.2 Sample Preparations 133
6.1.2 Molecular Weight 134
6.2 Small Angle Neutron Scattering (SANS) 136
6.2.1 Experimental Apparatus: Small Angle Neutron Diffractometer 137
6.2.2 Molecular Weight 138
xii
6.2.2.1 The Factors A and N 139
6.2.2.2 Relation Between 2b and Molecular Weight 140
6.2.2.3 Relation Between V and Mr 140
6.2.2.4 Multicomponent Particles 140
6.2.2.5 Rodlike Particles 142
6.2.2.6 Concentration of the Sample 142
6.3 Classical Light Scattering 142
6.3.1 Theory 143
6.3.2 Radius of Gyration 144
6.3.3 Types of Measurement 146
6.3.3.1 Turbidimetry 146
6.3.3.2 Low Angle Light Scattering 146
6.3.3.3 Multiangle Light Scattering 146
6.3.4 Experimental Considerations 147
6.4 Electron Microscopy 148
6.4.1 Quantitative Transmission Electron Microscopy (QTEM) 150
6.4.1.1 The Integrating Photometer 151
6.4.1.2 Standardization 154
6.4.1.3 Procedure and Treatment of Data to Obtain the Average Mass
of Virus Particles 156
6.4.1.4 High Voltage Quantitative Transmission Electron Microscopy 159
6.4.2 Counting of Particles to Obtain the Mean Molecular Weight 160
6.4.3 Quantitative Scanning Transmission Electron Microscopy 161
7 Hydrodynamic Sizing and Solvation 163
7.1 Laser Light Scattering 164
7.2 High Flux X Ray and Neutron Solution Scattering 164
7.3 Resistive Pulse Technique 164
7.4 Hydrodynamic Radii and Solvation 165
8 Available Resources 169
8.1 Instrument Use 169
8.1.1 Sedimentation Experiments 169
8.1.2 Scattering Studies 170
8.1.3 National Resources in Electron Microscopy 170
8.1.3.1 Remote Operation of Electron Microscopes 170
8.1.3.2 World Wide Web Controlled Scanning Electron
Microscope Availability 172
8.2 Computer Programs 172
8.3 Databases 173
8.3.1 A Database for Viruses 173
8.3.2 Databases for Viral Components: Proteins and Nucleic Acids 174
References 177
Index 199
xiii
|
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| discipline | Biologie Chemie Medizin |
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| spelling | Mazzone, Horace M. Verfasser aut CRC handbook of viruses mass-molecular weight values and related properties Horace M. Mazzone Boca Raton [u.a.] CRC Press 1998 XIII, 206 S. Ill. txt rdacontent n rdamedia nc rdacarrier Virologie moléculaire - Guides, manuels, etc Virus - Guides, manuels, etc Molecular Weight Molecular virology Handbooks, manuals, etc Virology instrumentation Viruses Handbooks, manuals, etc Viruses chemistry Viruses isolation & purification Viruses ultrastructure Molekülmasse (DE-588)4170374-1 gnd rswk-swf Viren (DE-588)4063589-2 gnd rswk-swf Molekulargewichtsbestimmung (DE-588)4170395-9 gnd rswk-swf (DE-588)4151278-9 Einführung gnd-content Viren (DE-588)4063589-2 s DE-604 Molekülmasse (DE-588)4170374-1 s Molekulargewichtsbestimmung (DE-588)4170395-9 s HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008267170&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mazzone, Horace M. CRC handbook of viruses mass-molecular weight values and related properties Virologie moléculaire - Guides, manuels, etc Virus - Guides, manuels, etc Molecular Weight Molecular virology Handbooks, manuals, etc Virology instrumentation Viruses Handbooks, manuals, etc Viruses chemistry Viruses isolation & purification Viruses ultrastructure Molekülmasse (DE-588)4170374-1 gnd Viren (DE-588)4063589-2 gnd Molekulargewichtsbestimmung (DE-588)4170395-9 gnd |
| subject_GND | (DE-588)4170374-1 (DE-588)4063589-2 (DE-588)4170395-9 (DE-588)4151278-9 |
| title | CRC handbook of viruses mass-molecular weight values and related properties |
| title_auth | CRC handbook of viruses mass-molecular weight values and related properties |
| title_exact_search | CRC handbook of viruses mass-molecular weight values and related properties |
| title_full | CRC handbook of viruses mass-molecular weight values and related properties Horace M. Mazzone |
| title_fullStr | CRC handbook of viruses mass-molecular weight values and related properties Horace M. Mazzone |
| title_full_unstemmed | CRC handbook of viruses mass-molecular weight values and related properties Horace M. Mazzone |
| title_short | CRC handbook of viruses |
| title_sort | crc handbook of viruses mass molecular weight values and related properties |
| title_sub | mass-molecular weight values and related properties |
| topic | Virologie moléculaire - Guides, manuels, etc Virus - Guides, manuels, etc Molecular Weight Molecular virology Handbooks, manuals, etc Virology instrumentation Viruses Handbooks, manuals, etc Viruses chemistry Viruses isolation & purification Viruses ultrastructure Molekülmasse (DE-588)4170374-1 gnd Viren (DE-588)4063589-2 gnd Molekulargewichtsbestimmung (DE-588)4170395-9 gnd |
| topic_facet | Virologie moléculaire - Guides, manuels, etc Virus - Guides, manuels, etc Molecular Weight Molecular virology Handbooks, manuals, etc Virology instrumentation Viruses Handbooks, manuals, etc Viruses chemistry Viruses isolation & purification Viruses ultrastructure Molekülmasse Viren Molekulargewichtsbestimmung Einführung |
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