Gene expression systems: using nature for the art of expression
"Recombinant gene expression is the fastest growing area in the study of molecular biology. By the time the Human Genome Project is completed (about 2002), several thousand sequences will be known, but the purpose of the resultant expression products will remain a mystery. Gene discovery requir...
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
San Diego [u.a.]
Acad. Press
1999
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Summary: | "Recombinant gene expression is the fastest growing area in the study of molecular biology. By the time the Human Genome Project is completed (about 2002), several thousand sequences will be known, but the purpose of the resultant expression products will remain a mystery. Gene discovery requires efficient expression systems for determining the structure and function of gene products. Gene Expression Systems covers a variety of promoters and host organisms that researchers can tailor to their specific needs."--BOOK JACKET. |
| Physical Description: | XII, 480 S. Ill., graph. Darst. |
| ISBN: | 0122538404 |
Staff View
MARC
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| 245 | 1 | 0 | |a Gene expression systems |b using nature for the art of expression |c ed. by Joseph M. Fernandez ... |
| 264 | 1 | |a San Diego [u.a.] |b Acad. Press |c 1999 | |
| 300 | |a XII, 480 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 520 | 1 | |a "Recombinant gene expression is the fastest growing area in the study of molecular biology. By the time the Human Genome Project is completed (about 2002), several thousand sequences will be known, but the purpose of the resultant expression products will remain a mystery. Gene discovery requires efficient expression systems for determining the structure and function of gene products. Gene Expression Systems covers a variety of promoters and host organisms that researchers can tailor to their specific needs."--BOOK JACKET. | |
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Record in the Search Index
| _version_ | 1804126806137110528 |
|---|---|
| adam_text | CONTENTS
Contributors xi
Introduction
Joseph M. Fernandez and James P. Hoefflei
The Art of Expression 1
Purpose of This Book 2
Selecting a Suitable Expression System: Considerations 3
Genomics and the Future of Protein Expression Systems 5
Section I PROKARYOTIC EXPRESSION SYSTEMS
1. Gene Expression Systems Based on Bacteriophage
T7 RNA Polymerase
Russell Durbin
Introduction 10
T7 RNAP in Its Natural Habitat 11
Biochemistry of T7 RNAP 14
T7 Based Gene Expression Systems 24
Conclusions 36
References 37
2. Expression Vectors Employing the tic Promoter
Jiirgen Brosius
Introduction 46
Vectors for trc Driven Expression 51
Additional Hosts for trc Driven Expression 57
Outlook for Further Improvements of Expression Vectors
Employing the tic Promoter 58
References 59
vi Contents
3. Bacillus Expression: A Gram Positive Model
Eugenio Ferrari and Brian Miller
Introduction 66
Bacilli Used as Industrial Production Organisms 67
Genetic Manipulation in Bacilli 71
Plasmid Vectors 75
Transcriptional Regulation 77
Conclusions 81
Appendix 82
References 89
4. aiaB Expression System in Escherichia coli
Marc Better
Introduction 95
The araB Promoter and How It Works 97
Use of the ara System for Expression of Recombinant
Products 99
Conclusions 104
References 106
Section II EUKARYOTIC EXPRESSION SYSTEMS
5. Adenoviral Vectors for Protein Expression
Dan J. Von Seggern and Glen R. Nemerow
Adenovirus Biology 112
Adaptation of Adenovirus as a Gene Transfer
Vector 117
Construction of Adenovirus Expression Vectors 119
Applications of Adenoviral Vectors in Gene
Expression 128
Considerations in the Use of Adenovirus Expression
Vectors 138
Advantages and Disadvantages of Adenoviral
Vectors 141
Future Directions 143
Conclusions 144
References 145
Contents vii
6. Expression in the Methylotrophic Yeast Pichia pastohs
fames M. Ciegg
Introduction 158
Background Information 161
Construction of Expression Strains 166
Important Considerations in the Use of the Expression
System 175
Conclusion 183
References 184
7. Recombinant Protein Expression in Pichia tnethanolica
Christopher K. Raymond
Background 193
DNA Transformation 194
Expression Vector and Identification of an Expression
Strain 196
Gene Disruptions and Generation of a Protease Deficient
Strain 198
Fermentation 199
Evaluation of Protein Expression 199
Expression of the 65 kDa Isoform of Human Glutamate
Decarboxylase in P. pastoris and P. methanolica 202
Protein Secretion 202
Benefits and Liabilities of the P. methanolica Expression
System 205
Summary 206
Appendix 207
References 207
8. Cytomegalovirus Promoter for Expression in
Mammalian Cells
Mark F. Stinski
Introduction 212
The Cytomegalovirus Enhancer Containing Promoter 214
Positive Regulation 216
Experimental Procedures 219
Effects Upstream of the Cytomegalovirus Enhancer 221
Effects Downstream of the Cytomegalovirus Promoter 222
Negative Regulation 223
viii Contents
Conclusions 225
References 228
9. Inducible Mammalian Expression Systems
Marijane Russell
Background: Development of Inducible Expression
Systems 235
Literature Review 245
Author s Experience 248
Comparing Inducible Mammalian Expression Systems
Currently Available 250
Conclusions and Future Directions 250
References 253
10. Protein Expression in Mammalian Cells Using
Sindbis Virus
Robert P. Bennett
Introduction 260
Applications 267
Protein Expression 272
Comparisons with Other Systems 279
New Directions and Conclusions 282
References 283
Section III EXPRESSION IN INSECT SYSTEMS
11. Drosophila S2 System for Heterologous Gene Expression
Robert B. Kirkpatrick and Allan Shatzman
Introduction 290
Properties of S2 Line 290
Experimental Procedures 292
Literature Review 300
Choosing an Expression System 315
Future Directions 318
Conclusions 321
Appendix 321
References 325
Contents ix
12. Baculovirus Expression Vector System
Michael Galleno and August J. Sick
Introduction 332
Background 334
Basic Research of Baculoviruses as a Tool for Gene
Expression 347
Expression Examples 349
Guidelines to Optimize Heterologous Gene Expression in
Baculovirus 352
Summary and Future 354
Appendix 355
References 359
Section IV TRANSGENIC EXPRESSION
13. Recombinant Protein Expression in Transgenic Mice
Rula Abbud and John H. Nilson
Overview and Background 368
Transgenic Mice: General Overview 368
Examples of Recombinant Protein Expression in Transgenic
Mice 371
Modeling Human Disease through Targeted
Overexpression 378
Biomass: Transgenic Mice as Model Bioreactors 381
Future of Transgenic Mice Approaches: Need for Inducible
Expression 383
Conclusions 390
References 391
14. Expression of Recombinant Proteins in the Milk of
Transgenic Animals
H. M. Meade, Y. Echelazd, C. A. Ziomek, M. W. Young,
M. Harvey, E. S. Cole, S. Groet, T. E. Smith, and f. M. Curling
Introduction 400
Expression of Heterologous Proteins in Milk 401
Milk Specific Transgenes 403
Insertion of the Transgene into the Germ Line 405
x Contents
Transgenic Animal Production 408
Biosynthesis of Milk Proteins 409
Milk Secretion from the Mammary Gland 412
Lactation and Milk Output 412
Milk Composition and Purification of the Target
Protein 413
Quality Issues in Transgenic Production 417
Regulatory Considerations 418
Current Status and Future Directions 420
References 421
15. Recombinant Protein Expression in Plants
Andreas E. Voloudakis, Yanhai Yin, and Roger N. Beachy
General Introduction 430
Transformation Methods 431
Promoters Used for Recombinant Protein Accumulation
in Plants 435
Expression of Recombinant Proteins in Plants and
Agricultural Biotechnology 438
Recombinant Protein Expression in Plants to Obtain New
Products 442
Protein Targeting and Accumulation 447
Virus Mediated Expression Systems 448
Summary/Discussion 450
References 452
Index 463
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| spelling | Gene expression systems using nature for the art of expression ed. by Joseph M. Fernandez ... San Diego [u.a.] Acad. Press 1999 XII, 480 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier "Recombinant gene expression is the fastest growing area in the study of molecular biology. By the time the Human Genome Project is completed (about 2002), several thousand sequences will be known, but the purpose of the resultant expression products will remain a mystery. Gene discovery requires efficient expression systems for determining the structure and function of gene products. Gene Expression Systems covers a variety of promoters and host organisms that researchers can tailor to their specific needs."--BOOK JACKET. Animaux transgéniques ram Cellules eucaryotes ram Expression génique ram Genexpressie gtt Genregulatie gtt Insectes ram Procaryotes - Génétique ram Animals, Transgenic genetics Eukaryotic Cells Eukaryotic cells Gene Expression Gene Expression Regulation Gene expression Insects Genetics Prokaryotes Genetics Prokaryotic Cells Transgenic animals Genetics Genexpression (DE-588)4020136-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Genexpression (DE-588)4020136-3 s DE-604 Fernandez, Joseph M. Sonstige oth HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008260172&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Gene expression systems using nature for the art of expression Animaux transgéniques ram Cellules eucaryotes ram Expression génique ram Genexpressie gtt Genregulatie gtt Insectes ram Procaryotes - Génétique ram Animals, Transgenic genetics Eukaryotic Cells Eukaryotic cells Gene Expression Gene Expression Regulation Gene expression Insects Genetics Prokaryotes Genetics Prokaryotic Cells Transgenic animals Genetics Genexpression (DE-588)4020136-3 gnd |
| subject_GND | (DE-588)4020136-3 (DE-588)4143413-4 |
| title | Gene expression systems using nature for the art of expression |
| title_auth | Gene expression systems using nature for the art of expression |
| title_exact_search | Gene expression systems using nature for the art of expression |
| title_full | Gene expression systems using nature for the art of expression ed. by Joseph M. Fernandez ... |
| title_fullStr | Gene expression systems using nature for the art of expression ed. by Joseph M. Fernandez ... |
| title_full_unstemmed | Gene expression systems using nature for the art of expression ed. by Joseph M. Fernandez ... |
| title_short | Gene expression systems |
| title_sort | gene expression systems using nature for the art of expression |
| title_sub | using nature for the art of expression |
| topic | Animaux transgéniques ram Cellules eucaryotes ram Expression génique ram Genexpressie gtt Genregulatie gtt Insectes ram Procaryotes - Génétique ram Animals, Transgenic genetics Eukaryotic Cells Eukaryotic cells Gene Expression Gene Expression Regulation Gene expression Insects Genetics Prokaryotes Genetics Prokaryotic Cells Transgenic animals Genetics Genexpression (DE-588)4020136-3 gnd |
| topic_facet | Animaux transgéniques Cellules eucaryotes Expression génique Genexpressie Genregulatie Insectes Procaryotes - Génétique Animals, Transgenic genetics Eukaryotic Cells Eukaryotic cells Gene Expression Gene Expression Regulation Gene expression Insects Genetics Prokaryotes Genetics Prokaryotic Cells Transgenic animals Genetics Genexpression Aufsatzsammlung |
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