Mycoplasma protocols:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
1998
|
| Series: | Methods in molecular biology
104 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XIII, 330 S. Ill., graph. Darst. |
| ISBN: | 0896035255 |
Staff View
MARC
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Record in the Search Index
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| adam_text | Contents
Preface v
Contributors xi
1 Introduction
Roger Miles and Robin Nicholas 1
2 The Medical Significance of Mycoplasmas
Paul Taylor 7
3 The Veterinary Significance of Mycoplasmas
Robin Nicholas 17
4 Recovery of Human Mycoplasmas
Paul Taylor 25
5 Recovery of Mycoplasmas from Animals
Robin Nicholas and Samantha Baker 37
6 Recovery of Mycoplasmas from Birds
Janet Bradbury 45
7 Cultivation of Ureaplasmas
Patricia Furr 53
8 Quality Control Testing of Mycoplasma Media
David Windsor and Helena Windsor 61
9 Biochemical Characteristics in Mycoplasma Identification
Jose B. Poveda 69
10 Enzyme Analysis: The Rationale and Use of Enzyme Assays
in Assigning Function to Gene Nucleotide Sequences
and the Procedures for the Assay of Three Enzymatic Functions
Conserved in Mollicutes
J. Dennis Pollack 79
11 Determination of Substrate Utilization Rates by Mycoplasmas
Roger Miles and Calista Agbanyim 95
viii Contents
12 Serological Identification of Mycoplasmas by Growth and Metabolism
Inhibition Tests
Jose B. Poveda and Robin Nicholas 105
13 Identification of Mycoplasmas by Dot Immunobinding on Membrane
Filtration (MF Dot)
Frangois Poumarat 113
14 Identification of Mycoplasmas by Immunofluorescence
Janet Bradbury 119
15 Diagnostic Application of Monoclonal Antibody (MAb) Based Sandwich
ELISAs
HywelJ. Ball and David Finlay 127
16 Immunohistochemical Staining of Fixed Tissues
Eugenio Scanziani 133
17 Extraction of DNA from Mycoplasmas
John B. Bashiruddin 141
18 Characterization of Mycoplasmas by PCR and Sequence Analysis
with Universal 16S rDNA Primers
Karl Erik Johansson, Malin Heldtander, and Bertil Pettersson 145
19 PCR and RFLP Methods for Specific Detection and Identification
of Mycoplasma mycoides subsp. mycoides SC
John B. Bashiruddin 167
20 Characterization of Mycoplasmas by RAPD Fingerprinting
Georges A. Rawadi 179
21 Classification of Isolates by DNA—DNA Hybridization
Konrad Sachse and Helmut Hotzel 189
22 Insertion Sequence Analysis
Joachim Frey 197
23 Detection of Mycoplasmas in Cell Cultures by Cultural Methods
Gerald K. Masover and Frances A. Becker 207
24 Detection of Mycoplasmas in Cell Culture by Fluorescence Methods
Gerald K. Masover and Frances A. Becker 217
25 Transformation of Mycoplasmas
F. Chris Minion and Paul A. Kapke 227
26 Transposon Mutagenesis
LeRoy L Voelker and Kevin Dybvig 235
27 Demonstration of Extrachromosomal Elements
LeRoy L. Voelker and Kevin Dybvig 239
Contents ix
28 Expression of Foreign Genes in Acholeplasma laidlawii
TanjaK. Jarhede and Ake Wieslander 247
29 Mycoplasma Gene Expression in Escherichia coli
F. Chris Minion 259
30 Polyacrylamide Gel Electrophoresis Separation of Whole Cell Proteins
Michael F. Duffy, Amir H. Noormohammadi, Nina Baseggio,
Glen F. Browning, and Philip F. Markham 267
31 Immunological and Biochemical Characterization of Membrane Proteins
Michael F. Duffy, Amir H. Noormohammadi, Nina Baseggio,
Glen F. Browning, and Philip F. Markham 279
32 Detection and Analysis of Mycoplasma Adhesins
Konrad Sachse 299
33 Transmission Electron Microscopy and Immunogold Staining
of Mollicute Surface Antigens
Gunna Christiansen and Svend Birkelund 309
Index 319
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| language | English |
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| physical | XIII, 330 S. Ill., graph. Darst. |
| publishDate | 1998 |
| publishDateSearch | 1998 |
| publishDateSort | 1998 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Mycoplasma protocols ed. by Roger Miles ... Totowa, NJ Humana Press 1998 XIII, 330 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 104 Mycoplasma cabt Mycoplasma diseases Laboratory manuals Mycoplasma Laboratory manuals Mycoplasma (DE-588)4170916-0 gnd rswk-swf Mycoplasmainfektion (DE-588)4196318-0 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Klinische Chemie (DE-588)4135255-5 gnd rswk-swf Mycoplasmainfektion (DE-588)4196318-0 s Methode (DE-588)4038971-6 s DE-604 Mycoplasma (DE-588)4170916-0 s Klinische Chemie (DE-588)4135255-5 s Miles, Roger Sonstige oth Methods in molecular biology 104 (DE-604)BV035362695 104 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008207015&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mycoplasma protocols Methods in molecular biology Mycoplasma cabt Mycoplasma diseases Laboratory manuals Mycoplasma Laboratory manuals Mycoplasma (DE-588)4170916-0 gnd Mycoplasmainfektion (DE-588)4196318-0 gnd Methode (DE-588)4038971-6 gnd Klinische Chemie (DE-588)4135255-5 gnd |
| subject_GND | (DE-588)4170916-0 (DE-588)4196318-0 (DE-588)4038971-6 (DE-588)4135255-5 |
| title | Mycoplasma protocols |
| title_auth | Mycoplasma protocols |
| title_exact_search | Mycoplasma protocols |
| title_full | Mycoplasma protocols ed. by Roger Miles ... |
| title_fullStr | Mycoplasma protocols ed. by Roger Miles ... |
| title_full_unstemmed | Mycoplasma protocols ed. by Roger Miles ... |
| title_short | Mycoplasma protocols |
| title_sort | mycoplasma protocols |
| topic | Mycoplasma cabt Mycoplasma diseases Laboratory manuals Mycoplasma Laboratory manuals Mycoplasma (DE-588)4170916-0 gnd Mycoplasmainfektion (DE-588)4196318-0 gnd Methode (DE-588)4038971-6 gnd Klinische Chemie (DE-588)4135255-5 gnd |
| topic_facet | Mycoplasma Mycoplasma diseases Laboratory manuals Mycoplasma Laboratory manuals Mycoplasmainfektion Methode Klinische Chemie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008207015&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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