Protein phosphatase protocols:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
1998
|
| Series: | Methods in molecular biology
93 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XIII, 316 S. Ill., graph. Darst. |
| ISBN: | 0896034682 0896035387 |
Staff View
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| 245 | 1 | 0 | |a Protein phosphatase protocols |c ed. by John W. Ludlow |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 1998 | |
| 300 | |a XIII, 316 S. |b Ill., graph. Darst. | ||
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| 650 | 4 | |a Phosphoprotein Phosphatases |x physiology | |
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Record in the Search Index
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|---|---|
| adam_text | Contents
Preface v
Contributors xi
1 Prokaryotic Protein Serine/Threonine Phosphatases
Peter J. Kennelly 1
2 Protein Phosphatase Type 1 and Type 2A Assays
S. Derek Killilea, Qi Cheng, andZhi Xin Wang 23
3 Analyzing Gene Expression with the Use of Serine/Threonine
Phosphatase Inhibitors
Axel H. Shonthal 35
4 lnhibitor 1, a Regulator of Protein Phosphatase 1 Function
John H. Connor, Hai Quan, Carey Oliver,
and Shirish Shenolikar 41
5 I,PP2A and I2PP2A: Two Potent Protein Phosphatase 2A Specific
Inhibitor Proteins
Mei Li and Zahi Damuni 59
6 Control of PP1 Activity Through Phosphorylation
by Cyclin Dependent Kinases
Norbert Berndt 67
7 Regulation of Neuronal PP1 and PP2A During Development
Elizabeth Collins and Alistair T. R. Sim 79
8 PTPA Regulating PP2A as a Dual Specificity Phosphatase
Veerle Janssens, Christine Van Hoof, Wilfried Merlevede,
and Jozef Goris 103
9 Microinjection and Immunological Methods in the Analysis
of Type 1 and 2A Protein Phosphatases from Mammalian Cells
Patric Turowski and Ned J. C. Lamb 117
10 Use of Immunocomplexed Substrate for Detecting PP1 Activity
John W. Ludlow, Deirdre A. Nelson, and Nancy A. Krucher 137
vii
viii Contents
11 The Biochemical Identification and Characterization of New
Species of Protein Phosphatase 1
Monique Beullens, Willy Stalmans, and Mathieu Bollen 145
12 The Relationship Between Insulin Signaling and Protein
Phosphatase 1 Activation
Louis Ragolia and Najma Begum 157
13 Analysis of the Isoforms of Protein Phosphatase 1 (PP1)
Isoforms with Polyclonal Peptide Antibodies
Margherita Tognarini and Emma Villa Moruzzi 169
14 Expression of Mouse Protein Phosphatase 2C in Eschericia coli
and COS 7 Cells
Takayasu Kobayashi, Kazuyuki Kusuda, Motoko Ohnishi,
Naoki Chida, and Shinri Tamura 185
15 Expression of Functional Protein Phosphatase 1 Catalytic
Subunit in E. coli
Mariam Dohadwala and Norbert Berndt 191
16 Protein Phosphatase 2A and Protein Phosphatase X Genes
in Arabidopsis thaliana
Gemma Pujol, Albert Ferrer, and Joaquin Arino 201
17 Separation of Protein Phosphatase Type 2C Isozymes
by Chromatography on Blue Sepharose
Susanne Klumpp and Dagmar Selke 213
18 Chromatographic Isolation of PP2A from Limulus Lateral Eyes:
Conventional and Small Scale Methods
Samuel C. Edwards, Travis B. Van Dyke, Timothy H. Van Dyke,
and David L. Brautigan 219
19 Purification and Assay of the Ptc/Tpd1 Protein Phosphatase 2C
from the Yeast Saccharomyces cerevisiae
Matthew K. Robinson and Eric M. Phizicky 235
20 Molecular Cloning of Protein Phosphatase Type 2C Isoforms
from Retinal cDNA
Dagmar Selke, Susanne Klumpp, Benjamin Kaupp,
and Arnd Baumann 243
21 Analysis of Protein Interactions Between Protein Phosphatase 1
and Noncatalytic Subunits Using the Yeast Two Hybrid Assay
Nadja T. Ramaswamy, Brian K. Dalley, and John F. Cannon 251
22 Identifying Protein Phosphatase 2A Interacting Proteins Using
the Yeast Two Hybrid Method
Brent McCright and David M. Virshup 263
Contents ix
23 Protein Phosphatase 2A Regulatory Subunits: cDNA Cloning
and Analysis of mRNA Expression
Julie A. Zaucha, Ryan S. Westphal, and Brian E. Wadzinski 279
24 Synthetic Lethal Screening in Protein Phosphatase Pathways
Jianhong Zheng and John F. Cannon 293
25 The Search for the Biological Function of Novel Yeast Ser/Thr
Phosphatases
Joaquin Ariho, Francesc Posas, and Josep Clotet 305
Index 315
|
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| illustrated | Illustrated |
| indexdate | 2024-07-09T18:22:01Z |
| institution | BVB |
| isbn | 0896034682 0896035387 |
| language | English |
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| physical | XIII, 316 S. Ill., graph. Darst. |
| publishDate | 1998 |
| publishDateSearch | 1998 |
| publishDateSort | 1998 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Protein phosphatase protocols ed. by John W. Ludlow Totowa, NJ Humana Press 1998 XIII, 316 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 93 Proteïne-fosfatasen gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Phosphoprotein Phosphatases physiology Phosphoprotein phosphatases Laboratory manuals Methode (DE-588)4038971-6 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf Phosphatasen (DE-588)4140462-2 gnd rswk-swf Phosphoproteinphosphatase (DE-588)4248815-1 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s Phosphatasen (DE-588)4140462-2 s Methode (DE-588)4038971-6 s DE-604 Phosphoproteinphosphatase (DE-588)4248815-1 s Ludlow, John W. Sonstige oth Methods in molecular biology 93 (DE-604)BV035362695 93 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008206975&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Protein phosphatase protocols Methods in molecular biology Proteïne-fosfatasen gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Phosphoprotein Phosphatases physiology Phosphoprotein phosphatases Laboratory manuals Methode (DE-588)4038971-6 gnd Proteine (DE-588)4076388-2 gnd Phosphatasen (DE-588)4140462-2 gnd Phosphoproteinphosphatase (DE-588)4248815-1 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4076388-2 (DE-588)4140462-2 (DE-588)4248815-1 (DE-588)4143413-4 |
| title | Protein phosphatase protocols |
| title_auth | Protein phosphatase protocols |
| title_exact_search | Protein phosphatase protocols |
| title_full | Protein phosphatase protocols ed. by John W. Ludlow |
| title_fullStr | Protein phosphatase protocols ed. by John W. Ludlow |
| title_full_unstemmed | Protein phosphatase protocols ed. by John W. Ludlow |
| title_short | Protein phosphatase protocols |
| title_sort | protein phosphatase protocols |
| topic | Proteïne-fosfatasen gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Phosphoprotein Phosphatases physiology Phosphoprotein phosphatases Laboratory manuals Methode (DE-588)4038971-6 gnd Proteine (DE-588)4076388-2 gnd Phosphatasen (DE-588)4140462-2 gnd Phosphoproteinphosphatase (DE-588)4248815-1 gnd |
| topic_facet | Proteïne-fosfatasen Wetenschappelijke technieken Wissenschaftliches Arbeiten Phosphoprotein Phosphatases physiology Phosphoprotein phosphatases Laboratory manuals Methode Proteine Phosphatasen Phosphoproteinphosphatase Aufsatzsammlung |
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