Practical applications of plant molecular biology:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
London [u.a.]
Chapman & Hall
1997
|
| Edition: | 1. ed. |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XVI, 258 S. Ill., graph. Darst. |
| ISBN: | 0412732106 0412732203 |
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| adam_text | 2M- 0&66 PRACTICAL APPLICATIONS OF PLANT MOLECULAR BIOLOGY R. J. HENRY
PROFESSOR OF PLANT CONSERVATION MOLECULAR GENETICS AT SOUTHERN CROSS
UNIVERSITY, AUSTRALIA CHAPMAN & HALL LONDON * WEINHEIM * NEW YORK *
TOKYO * MELBOURNE * MADRAS CONTENTS PREFACE XIII ACKNOWLEDGEMENTS XV 1
IDENTIFICATION OF PLANTS USING MOLECULAR TECHNIQUES 1 CHAPTER OUTLINE 3
1.1 MOLECULAR TECHNIQUES FOR PLANT IDENTIFICATION 3 1.1.1 INTRODUCTION 3
1.1.2 RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) AND OTHER
HYBRIDIZATION-BASED METHODS 6 1.1.3 RANDOM AMPLIFIED POLYMORPHIC DNA
(RAPD) 8 1.1.4 SIMPLE SEQUENCE REPEATS (SSR) 11 1.1.5 AMPLIFICATION
FRAGMENT LENGTH POLYMORPHISM (AFLP) 13 1.1.6 RIBOSOMAL GENE ANALYSIS 16
1.1.7 STORAGE PROTEIN GENE ANALYSIS 18 1.1.8 ALPHA-AMYLASE GENE ANALYSIS
20 1.1.9 PCR OF OTHER GENES 20 1.1.10 INTRON SPLICE JUNCTION PRIMERS 21
1.1.11 SEQUENCE TAGGED SITE (STS) AND SEQUENCE- CHARACTERIZED AMPLIFIED
REGION (SCAR) 22 1.1.12 SPECIFIC DETECTION OF PCR PRODUCTS 23 1.1.13
ANALYSIS OF SEQUENCE VARIATION IN PCR PRODUCTS 27 1.2 MOLECULAR METHODS
TO PROTECT PLANT BREEDERS RIGHTS 29 1.2.1 PRINCIPLES OF PLANT VARIETY
PROTECTION 29 1.2.2 TECHNIQUES TO VERIFY VARIETAL IDENTITY, PURITY AND
STABILITY 29 1.2.3 EXAMPLES 31 1.3 IDENTIFICATION OF PLANT PATHOGENS 33
1.3.1 NEED FOR PATHOGEN IDENTIFICATION 33 1.3.2 TECHNIQUES FOR PATHOGEN
IDENTIFICATION 33 1.3.3 EXAMPLES OF MOLECULAR METHODS FOR PLANT PATHOGEN
DIAGNOSIS 35 1.4 COMMERCIAL, INDUSTRIAL, FORENSIC AND OTHER PLANT
IDENTIFICATION 37 1.4.1 INTRODUCTION 37 VIII CONTENTS 1.4.2
IDENTIFICATION OF HORTICULTURAL PLANTS 37 1.4.3 SPECIES COMPOSITION OF
FOOD 38 1.4.4 VARIETAL IDENTITY AND PURITY IN GRAIN PROCESSING 41 1.4.5
IDENTITY OF GRAPE VARIETIES 42 1.4.6 DETECTING OFF-TYPES IN PLANT
PROPAGATION BY TISSUE CULTURE 45 1.4.7 OTHER APPLICATIONS 45 REFERENCES
50 2 ESTIMATION OF GENETIC VARIATION IN PLANTS USING MOLECULAR
TECHNIQUES 57 CHAPTER OUTLINE 59 2.1 INTRODUCTION 59 2.2 MOLECULAR
METHODS FOR MEASURING GENETIC VARIATION 63 2.2.1 MOLECULAR METHODS
AVAILABLE 63 2.2.2 DATA ANALYSIS 64 2.3 PHYLOGENETIC ANALYSIS OF PLANTS
USING MOLECULAR TECHNIQUES 68 2.3.1 INTRODUCTION 68 2.3.2 ANALYSIS OF
MITOCHONDRIAL DNA 69 2.3.3 ANALYSIS OF CHLOROPLAST GENES 71 2.3.4
ANALYSIS OF NUCLEAR GENES 72 2.4 APPLYING MOLECULAR TECHNIQUES TO PLANT
POPULATION GENETICS 75 2.5 APPLYING MOLECULAR TECHNIQUES TO THE
MANAGEMENT OF WILD PLANTS 79 2.5.1 INTRODUCTION 79 2.5.2 EXAMPLES 79 2.6
USING MOLECULAR TECHNIQUES TO MANAGE PLANT GENETIC RESOURCES 83 2.6.1
INTRODUCTION 83 2.6.2 PLANT GENETIC RESOURCE COLLECTIONS . 85 2.6.3
APPLICATIONS IN PLANT IMPROVEMENT 89 2.7 GENETIC DIVERSITY IN PLANT
PATHOGENS 89 2.7.1 INTRODUCTION 89 2.7.2 ANALYSIS OF GENETIC DIVERSITY
IN PLANT PATHOGENS 90 REFERENCES 93 3 MOLECULAR MARKERS IN PLANT
IMPROVEMENT 99 CHAPTER OUTLINE 101 CONTENTS I 3.1 INTRODUCTION 101 3.2
MOLECULAR MARKER TECHNIQUES AND THEIR APPLICATION IN PLANT IMPROVEMENT
103 3.2.1 INTRODUCTION 103 3.2.2 EXAMPLES OF APPLYING MOLECULAR MARKERS
103 3.3 MARKER-ASSISTED SELECTION IN A PLANT BREEDING PROGRAMME 105
3.3.1 INTRODUCTION 105 3.3.2 MOLECULAR MARKER MAPS OF PLANT GENOMES 105
3.3.3 LINKAGE OF MOLECULAR MARKERS TO USEFUL TRAITS 107 3.3.4 EXAMPLES
OF APPLICATION IN A PLANT BREEDING PROGRAMME 111 3.4 MARKER-ASSISTED
BREEDING TO IMPROVE PRODUCT QUALITY 113 3.4.1 INTRODUCTION 113 3.4.2
EXAMPLES 114 3.5 MARKER-ASSISTED BREEDING FOR DISEASE-RESISTANT PLANTS
123 3.5.1 INTRODUCTION 123 3.5.2 EXAMPLES 124 3.6 MARKER-ASSISTED
BREEDING FOR IMPROVED PLANT PERFORMANCE 127 3.6.1 INTRODUCTION 127 3.6.2
EXAMPLES 127 REFERENCES 129 4 PLANT TRANSFORMATION 13 5 CHAPTER OUTLINE
137 4.1 INTRODUCTION 137 4.2 TECHNIQUES FOR PLANT TRANSFORMATION 137
4.2.1 AGROBACTERIUM-MEDIATED TRANSFORMATION 137 4.2.2 DIRECT DNA
TRANSFER 138 4.2.3 PREPARING GENES FOR INTRODUCTION INTO PLANTS 138
4.2.4 LIMITATIONS OF TRANSFORMATION TECHNIQUES 139 4.3 USING
TRANSFORMATION TO STUDY PLANT PHYSIOLOGY AND BIOCHEMISTRY 142 4.4
IMPROVING PRODUCT QUALITY BY TRANSFORMATION 144 4.4.1 INTRODUCTION 144
4.4.2 NUTRITIONAL QUALITY 145 4.4.3 FUNCTIONAL QUALITY 146 4.5 PRODUCING
PEST- AND DISEASE-RESISTANT TRANSGENIC PLANTS 155 4.5.1 INSECT
RESISTANCE 155 4.5.2 DISEASE RESISTANCE 156 X CONTENTS 4.6 PRODUCING
HERBICIDE-RESISTANT PLANTS 158 4.6.1 HERBICIDE RESISTANCE AS A
CONSEQUENCE OF TRANSFORMATION TECHNIQUE 158 4.6.2 HERBICIDE-RESISTANT
PLANTS IN AGRICULTURE 158 4.6.3 RESISTANCE TO PARASITIC PLANTS 160 4.7
PRODUCING PLANTS WITH RESISTANCE TO ABIOTIC STRESS 164 4.8 MANIPULATING
REPRODUCTIVE BEHAVIOUR IN PLANTS 165 4.9 PRODUCING RECOMBINANT PROTEINS
IN PLANTS 165 4.10 USING PLANTS TO DELIVER ANTIGENS 165 4.11 CONSUMER
ACCEPTANCE OF TRANSGENIC PLANTS 166 4.12 INTELLECTUAL PROPERTY ISSUES
166 4.13 REGULATION OF TRANSGENIC PLANTS 167 REFERENCES 170 5 USEFUL
ROUTINE PROTOCOLS IN PLANT MOLECULAR BIOLOGY 175 CHAPTER OUTLINE 177 5.1
INTRODUCTION 177 5.2 ISOLATION OF PLANT DNA 177 5.2.1 INTRODUCTION 177
5.2.2 SAMPLE COLLECTION AND STORAGE 178 5.2.3 STANDARD PROCEDURES FOR
GENERAL USE 178 5.2.4 SIMPLE PROCEDURES FOR USE IN PCR SCREENING 180
5.2.5 REMOVAL OF SPECIFIC CONTAMINANTS 181 5.2.6 ESTIMATION OF THE
QUANTITY OF DNA OBTAINED 182 5.2.7 ISOLATION OF DNA FROM MITOCHONDRIA
AND CHLOROPLASTS 183 5.3 BLOTTING 183 5.3.1 SOUTHERN 183 5.3.2 NORTHERN
188 5.3.3 WESTERN 188 5.4 PCR PROTOCOLS 188 5.4.1 INTRODUCTION 188 5.4.2
ARBITRARY PRIMER PROTOCOLS 189 5.4.3 SPECIFIC PCR 191 5.5 GEL
ELECTROPHORESIS 192 5.5.1 AGAROSE GEL ELECTROPHORESIS 192 5.5.2
POLYACRYLAMIDE GEL ELECTROPHORESIS 194 5.5.3 PULSED FIELD
ELECTROPHORESIS 195 CONTENT 5.6 DNA SEQUENCING AND SEQUENCE ANALYSIS 195
5.6.1 DNA SEQUENCING 195 5.6.2 SEQUENCE ANALYSIS 197 5.7 CLONING IN
PLASMID VECTORS 197 5.7.1 INTRODUCTION 197 5.7.2 CLONING OF PCR PRODUCTS
203 5.7.3 EXPRESSION OF PLANT GENES IN BACTERIA 204 5.8 PLANT
TRANSFORMATION 207 5.8.1 AGROBACTERIUM-MEDIATED 207 5.8.2 MICROPARTICLE
BOMBARDMENT 207 5.8.3 ASSAY OF MARKER GENES 209 5.9 LIBRARY PRODUCTION
213 5.9.1 GENOMIC LIBRARY PREPARATION 213 5.9.2 CDNA LIBRARY PRODUCTION
213 5.9.3 BAC AND YAC LIBRARY PRODUCTION 213 5.9.4 SCREENING OF
LIBRARIES 214 5.10 CLONING PLANT GENES 214 5.11 MISCELLANEOUS METHODS
215 REFERENCES 218 APPENDICES 221 A PLANT GENOME INFORMATION 221 B
CLASSIFICATION OF FLOWERING PLANTS 223 C PLANTS REPRESENTING IMPORTANT
SOURCES OF FOOD 237 D NUCLEAR DNA CONTENT OF IMPORTANT PLANT SPECIES 240
E PATENTABLE SUBJECT MATTER IN PLANT MOLECULAR BIOLOGY 242 F GUIDELINES
FOR LABORATORY NOTEBOOKS 243 G REFERENCE INFORMATION FOR PLANT MOLECULAR
BIOLOGY 243 CODONS 243 CODES FOR REDUNDANT NUCLEOTRODES IN SEQUENCES 243
AMINO ACIDS 244 PREFIXES FOR UNITS 245 COMMON ABBREVIATIONS FOR NUCLEIC
ACIDS 245 INTERNATIONAL SOCIETY FOR PLANT MOLECULAR BIOLOGY 245 PLANT
GENOME CONFERENCES 246 RESTRICTION ENZYMES 246 BUFFERS 250 NOMENCLATURE
OF PLANT GENES 250 PLANT CULTURE MEDIA 251 REFERENCES 252 INDEX 253
|
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| discipline | Biologie Agrarwissenschaft Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau Pflanzenbau |
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| indexdate | 2024-07-09T18:15:09Z |
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| isbn | 0412732106 0412732203 |
| language | English |
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| spelling | Henry, Robert J. 1955- Verfasser (DE-588)1030596263 aut Practical applications of plant molecular biology R. J. Henry 1. ed. London [u.a.] Chapman & Hall 1997 XVI, 258 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Botanica larpcal Molecular biology Plant molecular biology Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Anwendung (DE-588)4196864-5 gnd rswk-swf Pflanzen (DE-588)4045539-7 gnd rswk-swf Pflanzenzüchtung (DE-588)4045599-3 gnd rswk-swf Pflanzenzüchtung (DE-588)4045599-3 s DE-604 Pflanzen (DE-588)4045539-7 s Molekularbiologie (DE-588)4039983-7 s Anwendung (DE-588)4196864-5 s GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007928654&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Henry, Robert J. 1955- Practical applications of plant molecular biology Botanica larpcal Molecular biology Plant molecular biology Molekularbiologie (DE-588)4039983-7 gnd Anwendung (DE-588)4196864-5 gnd Pflanzen (DE-588)4045539-7 gnd Pflanzenzüchtung (DE-588)4045599-3 gnd |
| subject_GND | (DE-588)4039983-7 (DE-588)4196864-5 (DE-588)4045539-7 (DE-588)4045599-3 |
| title | Practical applications of plant molecular biology |
| title_auth | Practical applications of plant molecular biology |
| title_exact_search | Practical applications of plant molecular biology |
| title_full | Practical applications of plant molecular biology R. J. Henry |
| title_fullStr | Practical applications of plant molecular biology R. J. Henry |
| title_full_unstemmed | Practical applications of plant molecular biology R. J. Henry |
| title_short | Practical applications of plant molecular biology |
| title_sort | practical applications of plant molecular biology |
| topic | Botanica larpcal Molecular biology Plant molecular biology Molekularbiologie (DE-588)4039983-7 gnd Anwendung (DE-588)4196864-5 gnd Pflanzen (DE-588)4045539-7 gnd Pflanzenzüchtung (DE-588)4045599-3 gnd |
| topic_facet | Botanica Molecular biology Plant molecular biology Molekularbiologie Anwendung Pflanzen Pflanzenzüchtung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007928654&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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