Signalübertragung von Memebranrezeptoren zu MAP-Kinasen über PI 3-kinase:
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| Format: | Book |
| Language: | German |
| Published: |
1997
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Jena, Univ., Diss., 1997 |
| Physical Description: | 83 Bl. graph. Darst. |
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MARC
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CONTENTS
I
CONTENTS /
1 INTRODUCTION 1
1.1 GENERAL OVERVIEW 1
1.2 TYROSINE KINASE RECEPTORS AND SIGNALLING PATHWAYS 2
1.2.1 ACTIVATION OF RECEPTOR TYROSINE KINASES AND INTERACTING PROTEINS 2
1.2.2 ADAPTOR PROTEINS 6
1.2.3 RAS-MAP KINASE PATHWAY ACTIVATION 7
1.3 G PROTEIN-COUPLED RECEPTORS AND SIGNALLING PATHWAYS REGULATING
GROWTH RESPONSES 9
1.4 ROLE OF PHOSPHOINOSITIDE 3-KINASE IN SIGNAL TRANSDUCTION 12
1.4.1 THE PHOSPHOINOSITIDE 3-KINASE FAMILY 14
1.4.2 ROLE OF PHOSPHOINOSITIDE 3-KINASESIN CELLULAR FUNTION 16
1.4.3 BIOCHEMICAL TARGETS OF PHOSPHOINOSITIDES 19
1.5 AIM OF THIS WORK 20
2 MATERIALS AND METHODS 21
2.1 MATERIALS 21
2.1.1 BACTERIAL STRAINS 21
2.1.2 ANTIBODIES 21
2.1.3 PLASMIDS 22
2.1.4 ENZYMES AND KITS 24
2.1.5 CHEMICALS 24
2.1.6 BACTERIAL SOLUTIONS AND MEDIA 25
2.1.7 CELL CULTURE MEDIA AND ADDITIVES 27
2.1.8 GENERAL SOLUTIONS 27
2.1.9 OTHER REAGENTS AND MATERIALS 29
2.2 METHODS 29
2.2.1 CONSTRUCTION OF EXPRESSION VECTORS 29
2.2.2 PREPARATION OF COMPETENT
E. COLI BACTERIAL CELLS AND
TRANSFORMATION WITH PLASMID DNA 30
2.2.3 ISOLATION OF PLASMID DNA 31
2.2.4 PRODUCTION AND PURIFICATION OF GST-ATF2 FUSION PROTEINS 32
2.2.4 DEAE-DEXTRAN TRANSFECTION OF COS-7 CELLS 33
2.2.5 KINASE ASSAYS 34
2.2.5.1 MAP KINASE ASSAY 34
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/951958887
CONTENTS
U
2.2.5.1 JNK KINASE ASSAY 34
2.2.5.1 MISCELLANEOUS KINASE ASSAY 35
2.2.6 WESTERN BLOTS 35
3 RESULTS 37
3.1 PHOSPHOINOSITIDE 3-KINASE Y LINKS G PROTEIN-COUPLED
RECEPTORS TO MAP KINASE PATHWAY 37
3.1.1 WORTMANNIN BLOCKS MAP KINASE ACTIVATION IN COS-7 CELLS 37
3.1.2 PI3KY INDUCES MAP KINASE ACTIVATION IN COS-7 CELLS 38
3.1.3 RAS AND SOS MEDIATE MAP KINASE ACTIVATION BY PI3KY 44
3.1.4 INVOLVEMENT OF SHE AND GRB2 IN MAP KINASE ACTIVATION BY PI3KY 46
3.2 REQUIREMENT OF PHOSPHOINOSITIDE 3-KINASE FOR ACTIVATION OF JNK 49
3.2.1 LIGAND-INDUCED TYROSINE PHOSPHORYLATION OF THE PPDGFR WT
LEADS TO ENHANCEMENT OF JNK ACTIVITY IN COS-7 CELLS 51
3.2.2 THE LIGAND-DEPENDENT ASSOCIATION OF PPDGFR WITH PI3KA IS
REQUIRED FOR JNK ACTIVATION 52
3.2.3 WORTMANNIN A POTENT INHIBITOR OF PI3K POTENTLY BLOCKS
THE LIGAND-DEPENDENT STIMULATION OF JNK ACTIVITY BY THE
PPDFGR WILD TYPE 54
3.2.4 DOMINANT NEGATIVE RAC AND RAS BUT NOT RHO OR CDC42 INHIBIT
ACTIVATION OF JNK BY P 110A 55
4 DISCUSSION 57
4.1 PHOSPHOINOSITIDE 3-KINASE Y LINKS G PROTEIN-COUPLED
RECEPTORS TO MAP KINASE PATHWAY 57
4.2 REQUIREMENT OF PI 3-KINASE FOR THE ACTIVATION OF JNK 65
5 REFERENCES 69
6 APPENDIX 77
6.1 ABBREVIATIONS 77
6.2 PUBLICATIONS 79
6.3 ACKNOWLEDGEMENTS 80
6.4 LEBENSLAUF 81
SELBSTANDIGKEITERKLARUNG 82
ERKLARUNG ZUR BEWERBUNG 83 |
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| id | DE-604.BV011665640 |
| illustrated | Illustrated |
| indexdate | 2024-10-09T16:07:05Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-007864655 |
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| physical | 83 Bl. graph. Darst. |
| publishDate | 1997 |
| publishDateSearch | 1997 |
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| spelling | Lopez-Ilasaca, Marco 1965- Verfasser (DE-588)115780556 aut Signalübertragung von Memebranrezeptoren zu MAP-Kinasen über PI 3-kinase von Marco Lopez-Ilasaca 1997 83 Bl. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Jena, Univ., Diss., 1997 Phosphoinositide (DE-588)4346034-3 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf Rezeptor-Tyrosinkinasen (DE-588)4342440-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Rezeptor-Tyrosinkinasen (DE-588)4342440-5 s Signaltransduktion (DE-588)4318717-1 s Phosphoinositide (DE-588)4346034-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007864655&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Lopez-Ilasaca, Marco 1965- Signalübertragung von Memebranrezeptoren zu MAP-Kinasen über PI 3-kinase Phosphoinositide (DE-588)4346034-3 gnd Signaltransduktion (DE-588)4318717-1 gnd Rezeptor-Tyrosinkinasen (DE-588)4342440-5 gnd |
| subject_GND | (DE-588)4346034-3 (DE-588)4318717-1 (DE-588)4342440-5 (DE-588)4113937-9 |
| title | Signalübertragung von Memebranrezeptoren zu MAP-Kinasen über PI 3-kinase |
| title_auth | Signalübertragung von Memebranrezeptoren zu MAP-Kinasen über PI 3-kinase |
| title_exact_search | Signalübertragung von Memebranrezeptoren zu MAP-Kinasen über PI 3-kinase |
| title_full | Signalübertragung von Memebranrezeptoren zu MAP-Kinasen über PI 3-kinase von Marco Lopez-Ilasaca |
| title_fullStr | Signalübertragung von Memebranrezeptoren zu MAP-Kinasen über PI 3-kinase von Marco Lopez-Ilasaca |
| title_full_unstemmed | Signalübertragung von Memebranrezeptoren zu MAP-Kinasen über PI 3-kinase von Marco Lopez-Ilasaca |
| title_short | Signalübertragung von Memebranrezeptoren zu MAP-Kinasen über PI 3-kinase |
| title_sort | signalubertragung von memebranrezeptoren zu map kinasen uber pi 3 kinase |
| topic | Phosphoinositide (DE-588)4346034-3 gnd Signaltransduktion (DE-588)4318717-1 gnd Rezeptor-Tyrosinkinasen (DE-588)4342440-5 gnd |
| topic_facet | Phosphoinositide Signaltransduktion Rezeptor-Tyrosinkinasen Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007864655&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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