Discovering neurons: the experimental basis of neuroscience
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York
Cold Spring Harbor Laboratory Press
1997
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIX, 420 S. Ill., graph. Darst. |
| ISBN: | 0879694556 |
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| 245 | 1 | 0 | |a Discovering neurons |b the experimental basis of neuroscience |c comp. and ed. by Carol Ann Paul |
| 264 | 1 | |a New York |b Cold Spring Harbor Laboratory Press |c 1997 | |
| 300 | |a XIX, 420 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
| _version_ | 1804126140973973504 |
|---|---|
| adam_text | Contents Preface, xvii
section I Neurons as Cells
Laboratory 1
Studying the Neuron Using Intracellular Recording: The Action Potential and Neuronal
Anatomy, 3
TO THE INSTRUCTOR, 4
WHAT YOU NEED TO KNOW, 7
Aplysia californica, 7
Intracellular Recording, 8
Resting Membrane Potential, 9
Action Potential, 10
Synaptic Potential, 10
Lucifer Yellow Dye, 10
IN THIS LAB, 12
PROTOCOL, 13
Materials, 13
Checking Equipment, 14
Making Electrodes, 15
Dissecting and Pinning the Ganglion, 16
Recording, 19
Comparing Electrophysiological Properties of Neurons, 21
Injecting Cells with Lucifer Yellow Dye, 22
WHERE DO YOU GO FROM HERE?, 24
IDEAS TO PONDER, 24
REFERENCES AND ADDITIONAL READING, 25
Laboratory 2
An Introduction to Neurochemistry: Acetylcholinesterase Assay, 27
TO THE INSTRUCTOR, 28
WHAT YOU NEED TO KNOW, 30
Acetylcholine, 30
Acetylcholinesterase, 31
vii
Contents
Biochemistry, 31
Enzyme Kinetics, 33
Enzyme Inhibition, 34
IN THIS LAB, 35
PROTOCOL, 36
Materials, 36
Assaying AChE in Different Brain Regions, 38
Comparing AChE Distribution in Tissue Homogenates with That in Sections Stained for AChE, 41
Determining the Effect of Tacrine on Enzyme Activity, 41
WHERE DO YOU GO FROM HERE?, 44
IDEAS TO PONDER, 44
REFERENCES AND ADDITIONAL READING, 45
Laboratory 3
Immunocytochemical Localization of Neuronal Transmitters: Two Methods for Identifying
Neurotransmitters In Situ, 47
TO THE INSTRUCTOR, 48
WHAT YOU NEED TO KNOW, 52
Definitions, 52
Labeling Methods, 53
Control Experiments, 58
Presoaking to Reduce Background, 58
Dilutions, 58
IN THIS LAB, 59
PROTOCOL, 60
Materials, 60
Tissue Dissection and Fixation, 61
Preparation of Flow Chart, 64
Immunocytochemical Double Labeling for Peptides (Two step Fluorescent Method), 64
Immunocytochemical Labeling for Serotonin (Three step ABC Method), 68
WHERE DO YOU GO FROM HERE?, 71
IDEAS TO PONDER, 71
DILUTION SUPPLEMENT, 72
REFERENCES AND ADDITIONAL READING, 75
Laboratory 4
Pharmacological Manipulation of Catecholamine Neurochemical Levels: An Analysis Using
HPLC,77
TO THE INSTRUCTOR, 78
WHAT YOU NEED TO KNOW, 82
Chromatography, 82
HPLC, 82
Catecholamines, 84
Amphetamines, 86
Protein Measurements, 87
IN THIS LAB, 87
PROTOCOL, 88
Materials, 88
Operating the HPLC and Selecting the Best Mobile Phase, 89
Contents
Analysis of Catecholamine and Metabolite Levels in Normal Mouse Striatum, 91
METH Injections and Analysis of Catecholamines and Metabolites in Striata of Amphetamine
treated Mice, 94
Protein Measurements, 94
WHERE DO YOU GO FROM HERE?, 96
IDEAS TO PONDER, 96
REFERENCES AND ADDITIONAL READING, 97
Laboratory 5
Calcium activated Chloride Conductance in Xenopus Oocytes: Introduction to Methods of
Molecular Neurobiology, 99
TO THE INSTRUCTOR, 100
WHAT YOU NEED TO KNOW, 104
Genetic Engineering in Neurobiology, 104
Endogenous Membrane Channels, 105
The Voltage Clamp Technique, 106
Membrane Capacitance, 107
Reversal Potentials, 108
Second Messenger activated and Inactivated Membrane Channels, 109
IN THIS LAB, 109
PROTOCOL, 110
Materials, 110
Setup, 111
Ca++ dependent Cr Channel Activity, 111
Voltage Clamp, 113
Measuring the Reversal Potential Using the Current Clamp, 114
Additional Experiments, 114
WHERE DO YOU GO FROM HERE?, 116
IDEAS TO PONDER, 116
REFERENCES AND ADDITIONAL READING, 117
section II Neurons Forming Connections
Laboratory 1
Synaptic Transmission and Synaptic Plasticity: The Neuromuscular Junction
of the Crayfish, 121
TO THE INSTRUCTOR, 122
WHAT YOU NEED TO KNOW, 126
The Crustacean Neuromuscular Junction, 126
Postsynaptic Response in the Crayfish, 126
The Superficial Flexor Muscle, 126
Synaptic Modulation, 127
IN THIS LAB, 128
PROTOCOL, 129
Materials, 129
Dissection Procedure, 130
Extracellular Motor Nerve Physiology, 132
Motor Nerve Anatomy, 133
Synaptic Physiology, 136
Intrinsic Synaptic Modulation, 138
Extrinsic Synaptic Modulation, 139
WHERE DO YOU GO FROM HERE?, 140
IDEAS TO PONDER, 140
REFERENCES AND ADDITIONAL READING, 141
Laboratory 2
Tracing Neural Connections: Thalamo cortical Interconnections in the Mammalian
Somatosensory System, 143
TO THE INSTRUCTOR, 144
WHAT YOU NEED TO KNOW, 147
Historical overview of Neuroanatomy, 147
Tracing Efferent Axons, 147
Tracing Afferent Axons, 149
Fluorescence Microscopy, 149
Somatosensory Cortex, 149
IN THIS LAB, 150
PROTOCOL, 151
Materials, 151
Injection of Tracers, 153
Perfusion, Fixation, and Removal of the Brain, 157
Tissue Preparation and Microscopic Analysis, 157
WHERE DO YOU GO FROM HERE?, 160
IDEAS TO PONDER, 160
REFERENCES AND ADDITIONAL READING, 161
Laboratory 3
Information Coding and Receptive Fields: Properties of Mammalian Neurons Recorded in Rat
Thalamus, 163
TO THE INSTRUCTOR, 164
WHAT YOU NEED TO KNOW, 166
Rat Whiskers as a Sensory Organ, 166
IN THIS LAB, 170
PROTOCOL, 171
Materials, 171
Equipment Setup, 172
Surgical Procedures, 172
Recording Procedures, 175
WHERE DO YOU GO FROM HERE?, 180
IDEAS TO PONDER, 180
REFERENCES AND ADDITIONAL READING, 181
Laboratory 4
Field Potential Recording in the Hippocampal Slice, 183
TO THE INSTRUCTOR, 184
WHAT YOU NEED TO KNOW, 190
Disadvantages of Recording from Whole Animals, 190
Contents
Slice Preparations, 190
The Hippocampus, 190
Evoked Field Potentials, 192
IN THIS LAB, 193
PROTOCOL, 194
Materials, 194
Determining Flow Rate of the ACSF, 195
Recording Field Potentials, 196
Possible Experiments, 198
Cleaning Up and Shutting Down the System, 200
WHERE DO YOU GO FROM HERE?, 201
IDEAS TO PONDER, 201
REFERENCES AND ADDITIONAL READING, 202
Laboratory 5
Long term Potentiation: Stimulating and Recording in the Hippocampus of an
Anesthetized Rat, 203
TO THE INSTRUCTOR, 204
WHAT YOU NEED TO KNOW, 207
Synaptic Plasticity, 207
Hippocampal Anatomy, 207
Field Potentials, 207
Evoked Field Potentials in LTP, 209
IN THIS LAB, 209
PROTOCOL, 211
Materials, 211
Equipment Setup, 212
Surgical Procedures, 212
WHERE DO YOU GO FROM HERE?, 218
IDEAS TO PONDER, 218
REFERENCES AND ADDITIONAL READING, 219
section III Neurons Coordinating Behavior
Laboratory 1
Observational Methods Used to Assess Behavior, 223
TO THE INSTRUCTOR, 224
WHAT YOU NEED TO KNOW, 225
The Behavioral Approach, 225
Inductive versus Deductive Methods, 225
Age and Sex of Subject, 225
The Rat as an Experimental Animal, 226
IN THIS LAB, 226
PROTOCOL, 227
Materials, 227
Daily Observations and Handling, 227
Neurological Testing, 230
Measures of Activity, 232
Other Behavioral Measures, 237
Suggested Experiments, 238
WHERE DO YOU GO FROM HERE?, 239
IDEAS TO PONDER, 239
REFERENCES AND ADDITIONAL READING, 240
Laboratory 2
Neuropharmacology: Comparison of Behavioral Response in Infant and Adult Rats after Drug
Administration, 241
TO THE INSTRUCTOR, 242
WHAT YOU NEED TO KNOW, 244
Life Cycle of a Neurotransmitter, 244
Catecholamines, 244
Function of NE and DA, 244
Receptors, Their Agonists, and Their Antagonists, 244
Drugs, 250
Drug Administration, 250
IN THIS LAB, 251
PROTOCOL, 252
Materials, 252
Overview of Behavioral Measures, 252
Procedure for Adult Rats, 253
Procedure for Rat Pups, 254
WHERE DO YOU GO FROM HERE?, 256
IDEAS TO PONDER, 256
REFERENCES AND ADDITIONAL READING, 257
Laboratory 3
Chronic Recording from Rat Brain: Hippocampal Theta Rhythm, 259
TO THE INSTRUCTOR, 260
WHAT YOU NEED TO KNOW, 265
Chronic EEG Recordings, 265
Theta Rhythm, 265
The Hippocampus, 265
IN THIS LAB, 266
PROTOCOL, 267
Materials, 267
Electrode, 268
Surgical Procedure, 268
Postoperative Care, 274
Training, 274
Physiological Data Collection, 276
WHERE DO YOU GO FROM HERE?, 279
IDEAS TO PONDER, 280
REFERENCES AND ADDITIONAL READING, 281
Contents
Laboratory 4
Effects of Lesion on Spatial and Nonspatial Learning, 283
TO THE INSTRUCTOR, 284
WHAT YOU NEED TO KNOW, 286
Lesion Methodologies, 286
Interpretation of Results, 286
The Double Dissociation, 286
Memory Systems, 287
The Behavioral Tasks, 287
IN THIS LAB, 288
PROTOCOL, 291
Materials, 291
Surgical Procedure, 292
Postoperative Care, 296
Behavioral Testing, 296
Lesion Verification and Histochemical Analysis, 299
WHERE DO YOU GO FROM HERE?, 300
IDEAS TO PONDER, 300
REFERENCES AND ADDITIONAL READING, 301
Laboratory 5
Control of the Ovulatory Cycle and Sexual Behavior in the Syrian Hamster, 303
TO THE INSTRUCTOR, 304
WHAT YOU NEED TO KNOW, 311
Hormonal Changes and the Estrous Cycle, 311
Neural Control of the Ovulatory Cycle, 312
Changes in Uterine Weight, 312
Verification of Estrous Cycle Stage, 313
IN THIS LAB, 315
PROTOCOL, 316
Materials, 316
Implantation of Subcutaneous Steroid Hormone Capsules, 318
Ovariectomy, 319
Vaginal Lavage, 320
Lordosis Testing, 320
Evaluation of Reproductive Tract and Ova Count, 322
WHERE DO YOU GO FROM HERE?, 328
IDEAS TO PONDER, 328
REFERENCES AND ADDITIONAL READING. 329
section IV Techniques
Protocol 1
Using Electrophysiological Equipment, 333
EQUIPMENT, 334
Oscilloscope, 334
Recording Systems, 336
Amplifier, 336
Audio Monitor, 335
Stimulator, 338
Cables and Connections, 339
The Faraday Cage, 341
EXTRACELLULAR AND INTRACELLULAR RECORDING, 343
Extracellular Recording, 343
Intracellular Recording, 343
EXERCISES, 346
Turning on the Oscilloscope and Finding the Beam, 346
Setting the Trigger, 347
Displaying a Known Voltage, 347
Storing a Signal, 347
Recording a Signal, 348
A Black Box Exercise: Determining the Properties of an Unknown Signal, 348
Preparing Microelectrodes Used in Intracellular Recording, 348
REFERENCES AND ADDITIONAL READING, 350
Protocol 2
Surgery Procedures, 351
IMPORTANT INFORMATION ABOUT ANIMAL USE, 362
SURGERY EQUIPMENT, 354
Equipment, 354
Surgical Instruments, 354
Surgical Supplies, 354
IMPORTANT INFORMATION ABOUT SURGERY, 355
Planning the Lab, 355
Animals and Allergies, 355
Sterility, 355
Anesthetics and Other Drugs, 355
The Injection, 358
Monitoring the Animal, 361
Surgery Procedures, 362
REFERENCES AND ADDITIONAL READING
Protocol 3
Perfusion and Sectioning, 369
PERFUSION, 370
Equipment, 370
Drugs, 371
Solutions, 371
Protocol, 371
DISSECTION PROCEDURE, 375
SECTIONING PROCEDURE, 376
Blocking and Mounting the Brain, 376
The Knife, 376
Sections, 377
Clean up, 377
Contents
Protocol 4
Staining: Golgi, Nissl, Acetylcholinesterase, and Choline Acetyltransferase, 379
SUBBING SLIDES, 380
Materials, 380
Method, 380
GOLGI SECTIONS OF ADULT RAT BRAINS, 382
Materials, 382
Method, 383
THE NISSL STAIN: A STAIN FOR CELL BODIES, 385
Materials, 385
Method, 385
ACETYLCHOLINESTERASE STAIN, 387
Materials, 387
Method, 388
CHOLINE ACETYLTRANSFERASE STAIN, 389
Materials, 389
Method, 390
Appendices
Appendix 1
Sources of Equipment, 395
Appendix 2
Addresses of Suppliers, 397
Appendix 3
Use of Animals in Experiments, 403
References and Additional Reading, 406
Index, 407
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| id | DE-604.BV011612382 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:12:44Z |
| institution | BVB |
| isbn | 0879694556 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-007822165 |
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| owner_facet | DE-355 DE-BY-UBR DE-11 DE-188 |
| physical | XIX, 420 S. Ill., graph. Darst. |
| publishDate | 1997 |
| publishDateSearch | 1997 |
| publishDateSort | 1997 |
| publisher | Cold Spring Harbor Laboratory Press |
| record_format | marc |
| spelling | Discovering neurons the experimental basis of neuroscience comp. and ed. by Carol Ann Paul New York Cold Spring Harbor Laboratory Press 1997 XIX, 420 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier neurons cabt neurophysiology cabt animal behaviour cabt rats cabt experimental animals cabt synaptic transmission Neurons Laboratory Manuals Neurons Laboratory manuals Neurophysiology Laboratory Manuals Neurophysiology Study and teaching (Higher) Neurophysiology education Neurosciences Laboratory Manuals Methode (DE-588)4038971-6 gnd rswk-swf Neurophysiologie (DE-588)4041897-2 gnd rswk-swf Neurophysiologie (DE-588)4041897-2 s Methode (DE-588)4038971-6 s DE-604 Paul, Carol Ann Sonstige oth HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007822165&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Discovering neurons the experimental basis of neuroscience neurons cabt neurophysiology cabt animal behaviour cabt rats cabt experimental animals cabt synaptic transmission Neurons Laboratory Manuals Neurons Laboratory manuals Neurophysiology Laboratory Manuals Neurophysiology Study and teaching (Higher) Neurophysiology education Neurosciences Laboratory Manuals Methode (DE-588)4038971-6 gnd Neurophysiologie (DE-588)4041897-2 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4041897-2 |
| title | Discovering neurons the experimental basis of neuroscience |
| title_auth | Discovering neurons the experimental basis of neuroscience |
| title_exact_search | Discovering neurons the experimental basis of neuroscience |
| title_full | Discovering neurons the experimental basis of neuroscience comp. and ed. by Carol Ann Paul |
| title_fullStr | Discovering neurons the experimental basis of neuroscience comp. and ed. by Carol Ann Paul |
| title_full_unstemmed | Discovering neurons the experimental basis of neuroscience comp. and ed. by Carol Ann Paul |
| title_short | Discovering neurons |
| title_sort | discovering neurons the experimental basis of neuroscience |
| title_sub | the experimental basis of neuroscience |
| topic | neurons cabt neurophysiology cabt animal behaviour cabt rats cabt experimental animals cabt synaptic transmission Neurons Laboratory Manuals Neurons Laboratory manuals Neurophysiology Laboratory Manuals Neurophysiology Study and teaching (Higher) Neurophysiology education Neurosciences Laboratory Manuals Methode (DE-588)4038971-6 gnd Neurophysiologie (DE-588)4041897-2 gnd |
| topic_facet | neurons neurophysiology animal behaviour rats experimental animals synaptic transmission Neurons Laboratory Manuals Neurons Laboratory manuals Neurophysiology Laboratory Manuals Neurophysiology Study and teaching (Higher) Neurophysiology education Neurosciences Laboratory Manuals Methode Neurophysiologie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007822165&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT paulcarolann discoveringneuronstheexperimentalbasisofneuroscience |