Marker enrichment and a high density map of the region carrying the R1 locus, which confers race specific resistance against phytophtora infestans in potato, using AFLP markers:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | German |
| Published: |
1995
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 83 S. Ill., graph. Darst. |
Staff View
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV010888670 | ||
| 003 | DE-604 | ||
| 007 | t | ||
| 008 | 960730s1995 gw ad|| m||| 00||| ger d | ||
| 016 | 7 | |a 947644768 |2 DE-101 | |
| 035 | |a (OCoLC)613734188 | ||
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| 041 | 0 | |a ger | |
| 044 | |a gw |c DE | ||
| 049 | |a DE-355 |a DE-83 |a DE-188 | ||
| 100 | 1 | |a Meksem, Khalid |d 1967- |e Verfasser |0 (DE-588)11446748X |4 aut | |
| 245 | 1 | 0 | |a Marker enrichment and a high density map of the region carrying the R1 locus, which confers race specific resistance against phytophtora infestans in potato, using AFLP markers |c vorgelegt von Khalid Meksem |
| 264 | 1 | |c 1995 | |
| 300 | |a 83 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Köln, Univ., Diss., 1995 | ||
| 650 | 0 | 7 | |a Resistenzgen |0 (DE-588)4280023-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Kartoffel |0 (DE-588)4029804-8 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Phytophthora infestans |0 (DE-588)4277191-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a RFLP |0 (DE-588)4238815-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Genkartierung |0 (DE-588)4123274-4 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Kartoffel |0 (DE-588)4029804-8 |D s |
| 689 | 0 | 1 | |a Phytophthora infestans |0 (DE-588)4277191-2 |D s |
| 689 | 0 | 2 | |a Resistenzgen |0 (DE-588)4280023-7 |D s |
| 689 | 0 | 3 | |a Genkartierung |0 (DE-588)4123274-4 |D s |
| 689 | 0 | 4 | |a RFLP |0 (DE-588)4238815-6 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007280771&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-007280771 | |
Record in the Search Index
| _version_ | 1807683962662813696 |
|---|---|
| adam_text |
CONTENT
PAGE
ABSTRACT
ABBREVATIONS
1
INTRODUCTION
.
5
2
MATERIALS
AND
METHODS
.
14
2.
1
MATERIAL
.
14
2.1.1
PLANT
MATERIAL
.
14
2.
1.2
BACTERIA
AND
CLONING
VECTOR
.
15
2.1.3
OLIGONUCLEOTIDES
.
15
2.
1.4
ENZYMES
AND
CHEMICALS
.
16
2.2
METHODS
.
17
2.
2.
1
POOL
CONSTRUCTION
.
17
2.
2.
1.
1
FIRST
POOL
CONSTRUCTION
.
17
2.
2.
1.2
SECOND
POOL
CONSTRUCTION
.
19
2.
2.
2
DNA-ISOLATION
.
21
2.
2.
2.
1
PLANT
GENOMIC
DNA
EXTRACTION
.
21
2.
2.
2.
2
PLASMID-DNA
ISOLATION
.
22
2.
2.
3
RFLP
ANALYSIS
.
22
2.
2.
3.
1
RESTRICTION
DIGEST
AND
PREPARATION
OF
DNA
PROBES
.
22
2.
2.
3.
2
GEL
ELECTROPHORETIC
DNA
SEPARATION
.
22
2.
2.
3.3
ELECTROTRANSFER
OF
DNA-FRAGMENT.
23
2.
2.
3.
4
PREPARATION
OF
PROBES
.
23
2.
2.
3.
5
HYBRIDIZATION
.
24
2.
2.
4
AFLP
ANALYSIS
.
25
2.
2.
4.
1
RESTRICTION
DIGEST
.
25
2.
2.
4.
2
LIGATION
OF
ADAPTORS
.
25
2.
2.
4.
3
SELECTION
OF
THE
BIOTINYLATED
DNA
FRAGMENTS
.
25
2.
2.
4.4
SELECTIVE
PREAMPLIFICATION
+1/
+1
.
26
2.
2.
4.
5
PRIMER-LABELLING
.
26
2.
2.
4.
6
SELECTIVE
RESTRICTION
FRAGMENT
AMPLIFICATION
.
27
2.
2.
4.
7
GEL
ANALYSIS
.
27
2.
2.
5
CLONING
OF
THE
AFLP
MARKERS
.
29
2.
2.
5.
1
VECTOR
PREPARATION
.
30
2.
2.
5.
2
AFLP
FRAGMENT
PREPARATION
.
31
2.
2.
5.
3
LIGATION
REACTION
.
31
2.
2.
5.
4
PREPARATION
OF
ELECTRO-COMPETENT
BACTERIA
CELLS
.
31
2.
2.
5.
5
TRANSFORMATION
AND
SELECTION
OF
THE
POSITIVE
CLONES
.
31
2.
2.
5.
6
SEQUENCING
.
31
3.
RESULTS
.
34
3.
1
GEL
ANALYSIS
.
34
3.
2
AFLP MARKERS
AND
BULKED
SEGREGANT
ANALYSIS
.
34
3.
2.
1
AFLP MARKERS
LINKED
TO
THE
RI
LOCUS
OBTAINED
AFTER
.
36
THE
FIRST
POOL
CONFIGURATION
SCREENING
3.
2.
2
AFLP
MARKERS
LINKED
TO
THE
RI
LOCUS
OBTAINED
AFTER
.
37
THE
SECOND
POOL
CONFIGURATION
SCREENING
3.
3
HIGH-DENSITY
MAP
OF
THE
SEGMENT
CARRYING
RI
LOCUS
.
41
3.
4
IDENTIFIED
AFLP
MARKERS
AND
MAP-BASED
CLONING
OF
.
43
THE
RI
LOCUS
3.
4.
1
CLONING
AND
ANALYSIS
OF
THE
AFLP1
MARKER
.
56
3.
4.
2
MOLECULAR
ANALYSIS
OF
THE
AFLP2
MARKER
.
56
3.
4.
3
MOLECULAR
CLONING
OF
THE
AFLP3
AND
AFLP4
MARKERS
.
59
4.
DISCUSSION
.
60
5.
ZUSAMMENFASSUNG
.
67
6.
REFERENCES
.
68 |
| any_adam_object | 1 |
| author | Meksem, Khalid 1967- |
| author_GND | (DE-588)11446748X |
| author_facet | Meksem, Khalid 1967- |
| author_role | aut |
| author_sort | Meksem, Khalid 1967- |
| author_variant | k m km |
| building | Verbundindex |
| bvnumber | BV010888670 |
| ctrlnum | (OCoLC)613734188 (DE-599)BVBBV010888670 |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV010888670 |
| illustrated | Illustrated |
| indexdate | 2024-08-18T00:42:46Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-007280771 |
| oclc_num | 613734188 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-83 DE-188 |
| owner_facet | DE-355 DE-BY-UBR DE-83 DE-188 |
| physical | 83 S. Ill., graph. Darst. |
| publishDate | 1995 |
| publishDateSearch | 1995 |
| publishDateSort | 1995 |
| record_format | marc |
| spelling | Meksem, Khalid 1967- Verfasser (DE-588)11446748X aut Marker enrichment and a high density map of the region carrying the R1 locus, which confers race specific resistance against phytophtora infestans in potato, using AFLP markers vorgelegt von Khalid Meksem 1995 83 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 1995 Resistenzgen (DE-588)4280023-7 gnd rswk-swf Kartoffel (DE-588)4029804-8 gnd rswk-swf Phytophthora infestans (DE-588)4277191-2 gnd rswk-swf RFLP (DE-588)4238815-6 gnd rswk-swf Genkartierung (DE-588)4123274-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Kartoffel (DE-588)4029804-8 s Phytophthora infestans (DE-588)4277191-2 s Resistenzgen (DE-588)4280023-7 s Genkartierung (DE-588)4123274-4 s RFLP (DE-588)4238815-6 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007280771&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Meksem, Khalid 1967- Marker enrichment and a high density map of the region carrying the R1 locus, which confers race specific resistance against phytophtora infestans in potato, using AFLP markers Resistenzgen (DE-588)4280023-7 gnd Kartoffel (DE-588)4029804-8 gnd Phytophthora infestans (DE-588)4277191-2 gnd RFLP (DE-588)4238815-6 gnd Genkartierung (DE-588)4123274-4 gnd |
| subject_GND | (DE-588)4280023-7 (DE-588)4029804-8 (DE-588)4277191-2 (DE-588)4238815-6 (DE-588)4123274-4 (DE-588)4113937-9 |
| title | Marker enrichment and a high density map of the region carrying the R1 locus, which confers race specific resistance against phytophtora infestans in potato, using AFLP markers |
| title_auth | Marker enrichment and a high density map of the region carrying the R1 locus, which confers race specific resistance against phytophtora infestans in potato, using AFLP markers |
| title_exact_search | Marker enrichment and a high density map of the region carrying the R1 locus, which confers race specific resistance against phytophtora infestans in potato, using AFLP markers |
| title_full | Marker enrichment and a high density map of the region carrying the R1 locus, which confers race specific resistance against phytophtora infestans in potato, using AFLP markers vorgelegt von Khalid Meksem |
| title_fullStr | Marker enrichment and a high density map of the region carrying the R1 locus, which confers race specific resistance against phytophtora infestans in potato, using AFLP markers vorgelegt von Khalid Meksem |
| title_full_unstemmed | Marker enrichment and a high density map of the region carrying the R1 locus, which confers race specific resistance against phytophtora infestans in potato, using AFLP markers vorgelegt von Khalid Meksem |
| title_short | Marker enrichment and a high density map of the region carrying the R1 locus, which confers race specific resistance against phytophtora infestans in potato, using AFLP markers |
| title_sort | marker enrichment and a high density map of the region carrying the r1 locus which confers race specific resistance against phytophtora infestans in potato using aflp markers |
| topic | Resistenzgen (DE-588)4280023-7 gnd Kartoffel (DE-588)4029804-8 gnd Phytophthora infestans (DE-588)4277191-2 gnd RFLP (DE-588)4238815-6 gnd Genkartierung (DE-588)4123274-4 gnd |
| topic_facet | Resistenzgen Kartoffel Phytophthora infestans RFLP Genkartierung Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007280771&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT meksemkhalid markerenrichmentandahighdensitymapoftheregioncarryingther1locuswhichconfersracespecificresistanceagainstphytophtorainfestansinpotatousingaflpmarkers |